Free radical research communications最新文献_第9页

The superoxide scavenging activity of dihydroquinoline type derivatives (CH402 and MTDQ-DA). 二氢喹啉类衍生物(CH402和MTDQ-DA)的超氧化物清除活性。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309056517

L Prònai, A Blåzovics, M Horvåth, I Lång, J Fehér

引用次数: 4

Cellularly generated active oxygen species and HeLa cell proliferation. 细胞生成活性氧与HeLa细胞增殖。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309111603

R H Burdon, V Gill

引用次数: 70

Nitric oxide interaction with lactoferrin and its production by macrophage cells studied by EPR and spin trapping. 利用EPR和自旋捕获技术研究一氧化氮与乳铁蛋白的相互作用及其在巨噬细胞中的产生。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309056s201

A J Carmichael, L Steel-Goodwin, B Gray, C M Arroyo

{"title":"Nitric oxide interaction with lactoferrin and its production by macrophage cells studied by EPR and spin trapping.","authors":"A J Carmichael, L Steel-Goodwin, B Gray, C M Arroyo","doi":"10.3109/10715769309056s201","DOIUrl":"https://doi.org/10.3109/10715769309056s201","url":null,"abstract":"The production of nitrate (NO3-) and nitrite (NO2-) from macrophage-derived NO was studied using EPR and spin trapping. The formation of NO3- was determined via EPR in reactions involving the iron-binding protein, lactoferrin. The formation of NO2- was determined via EPR/spin trapping in the reaction between NO2- and H2O2. Dissolved nitric oxide (NO.) was reacted with lactoferrin yielding an EPR spectrum (77 degrees K) different from the normal EPR spectrum obtained for lactoferrin, suggesting that NO. interacts with the ferric ions bound to lactoferrin forming a ferric-nitrosyl type complex. The EPR spectrum (77 degrees K) of this ferric-nitrosyl type complex was also observed in the supernatant fluid of macrophage cell suspensions following their stimulation with lipopolysaccharide (LPS). During LPS stimulation of macrophages, these cells generate NO. which in turn produces NO3- and NO2-. The ferric-nitrosyl type complex is formed in a reaction mixture containing apolactoferrin and bicarbonate following the reaction of Fe+2 with NO3-, generated from macrophage-derived NO(.), to produce Fe+3 and NO(.). Furthermore, in an acidic medium, NO2- reacts with H2O2 forming peroxynitrous acid (HOONO) which rapidly decomposes into hydroxyl radicals (.OH) and the nitrogen dioxide (NO2.) radical. In the supernatant fluid of LPS-stimulated macrophage suspensions, the production of .OH was verified by spin trapping using 5,5-dimethyl-1-pyrroline-1-oxide (DMPO) as the spin trap and ethanol as the .OH scavenger. The EPR spectra corresponding to the DMPO-OH and the DMPO-hydroxyethyl adducts were identified.(ABSTRACT TRUNCATED AT 250 WORDS)","PeriodicalId":12438,"journal":{"name":"Free radical research communications","volume":"19 Suppl 1 ","pages":"S201-9"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10715769309056s201","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19269698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Oligonucleotides site-specifically spin-labeled at 5'-terminal or internucleotide linkage and their use in gene analyses. 寡核苷酸在5'末端或核苷酸间链上特异性自旋标记及其在基因分析中的应用。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309056s117

A Murakami, M Mukae, S Nagahara, Y Konishi, H Ide, K Makino

{"title":"Oligonucleotides site-specifically spin-labeled at 5'-terminal or internucleotide linkage and their use in gene analyses.","authors":"A Murakami, M Mukae, S Nagahara, Y Konishi, H Ide, K Makino","doi":"10.3109/10715769309056s117","DOIUrl":"https://doi.org/10.3109/10715769309056s117","url":null,"abstract":"Spin-labeled oligonucleotides (S-probes) were synthesized and examined as DNA probes to monitor hybrid formation. TEMPO was introduced either at the internucleotide linkage of 5'-terminus (Type 1) or at the 5'-terminal hydroxyl group (Type 2) and both types of S-probes were used in this study. The presence of target DNA was detected in solution by EPR spectroscopy for both types of S-probes. Hybridization of the S-probes resulted in notable broadening of EPR line width, accompanied by a decrease in the EPR signal height ratio for I(-1)/I(0).I(-1)/I(0) of S-probes having no spacer between oligonucleotide and TEMPO decreased more markedly than that of S-probes with a spacer, indicating that TEMPO should be introduced to an oligonucleotide directly to monitor hybrid formation. When M13mp8 single-stranded DNA with or without an EcoRI recognition site was selected as a target DNA, hybrid formation was detected only for DNA containing EcoRI site in solution using spin-labeled oligonucleotides.","PeriodicalId":12438,"journal":{"name":"Free radical research communications","volume":"19 Suppl 1 ","pages":"S117-28"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10715769309056s117","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19271052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Interaction between allopurinol and copper: possible role in myocardial protection. 别嘌呤醇与铜的相互作用:在心肌保护中的可能作用。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309149909

S Malkiel, R Har-el, H Schwalb, G Uretzky, J B Borman, M Chevion

{"title":"Interaction between allopurinol and copper: possible role in myocardial protection.","authors":"S Malkiel, R Har-el, H Schwalb, G Uretzky, J B Borman, M Chevion","doi":"10.3109/10715769309149909","DOIUrl":"https://doi.org/10.3109/10715769309149909","url":null,"abstract":"Allopurinol, a potent inhibitor of xanthine oxidase, is known to effectively protect the heart against damage in patients undergoing cardiac bypass surgery. There is still an ambiguity concerning the presence of xanthine oxidase in the human heart. Thus, the mechanism underlying the protective effect of allopurinol is unclear. Transition metal ions, such as iron and copper, can participate in single-electron reactions and mediate the formation of oxygen-derived free radicals. In this study the interaction between allopurinol and Cu(II) was investigated. Spectrophotometric investigation shows that allopurinol (0-0.8 mM) form a 1:1 complex with Cu(II) ions (0-0.8 mM) with a specific absorbance peak at 364 nm. Also, the rate constant (k) for the copper-catalyzed aerobic oxidation of ascorbate was markedly decreased in the presence of allopurinol (from 0.068 min-1 to 0.014 min-1). Allopurinol substantially reduced the copper-mediated and ascorbate-driven DNA breakage. Spectrophotometric measurements did not indicate a specific interaction between iron ions and allopurinol. It is suggested that the beneficial effects of allopurinol during reperfusion of the heart could stem from its chelation of copper, yielding a complex with low redox activity.","PeriodicalId":12438,"journal":{"name":"Free radical research communications","volume":"18 1","pages":"7-15"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10715769309149909","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19336079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Antioxidant activities of dihydrolipoic acid and its structural homologues. 二氢硫辛酸及其结构同源物的抗氧化活性。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309147348

Y J Suzuki, M Tsuchiya, L Packer

{"title":"Antioxidant activities of dihydrolipoic acid and its structural homologues.","authors":"Y J Suzuki, M Tsuchiya, L Packer","doi":"10.3109/10715769309147348","DOIUrl":"https://doi.org/10.3109/10715769309147348","url":null,"abstract":"The relationships between structure and antioxidant activity of dihydrolipoic acid (DHLA) were studied using homologues of DHLA: bisonor-DHLA (a derivative which lacks two carbons in the hydrophobic tail), tetranor-DHLA (which lacks four carbons) and a methyl ester derivative. It was observed that: i) DHLA homologues with shorter hydrocarbon tails (i.e., bisnor- and tetranor-DHLA) had greater ability to quench superoxide radicals (O2-); ii) no differences among homologues with different chain lengths were found for peroxyl radical (ROO.) scavenging in aqueous solution, and iii) DHLA was the best membrane antioxidant in terms of ROO. scavenging and lipid peroxidation inhibition. Differences among the DHLA homologues in their antioxidant properties in polar and apolar environments generally agreed with differences in their partition coefficients. The methyl ester was the least effective antioxidant both in aqueous phase and in membranes. Tetranor-DHLA was found not only to be less effective in preventing ROO.-induced lipid peroxidation, but also to induce lipid peroxidation in the presence of residual iron. Thus, the complexity of biological systems seems to complicate generalizations on the correlation of molecular structure with antioxidant activity of DHLA.","PeriodicalId":12438,"journal":{"name":"Free radical research communications","volume":"18 2","pages":"115-22"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10715769309147348","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19372588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 70

Influence of hyperthyroidism on superoxide radical and hydrogen peroxide production by rat liver submitochondrial particles. 甲状腺机能亢进对大鼠肝脏亚线粒体颗粒产生超氧自由基和过氧化氢的影响。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309147500

V Fernández, L A Videla

引用次数: 102

Modulation of glutathione and glutathione dependent antioxidant enzymes in mouse heart following doxorubicin therapy. 阿霉素治疗后小鼠心脏谷胱甘肽和谷胱甘肽依赖性抗氧化酶的调节。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309056505

D L Gustafson, J D Swanson, C A Pritsos

{"title":"Modulation of glutathione and glutathione dependent antioxidant enzymes in mouse heart following doxorubicin therapy.","authors":"D L Gustafson, J D Swanson, C A Pritsos","doi":"10.3109/10715769309056505","DOIUrl":"https://doi.org/10.3109/10715769309056505","url":null,"abstract":"The toxicity of the antineoplastic agent doxorubicin (DOX) has been shown to be moderated by the antioxidant enzyme glutathione peroxidase. It has been reported that acute doses of DOX can cause an inhibition of glutathione peroxidase in cardiac tissue, that may render this tissue especially susceptible to further prooxidant damage. In this study, multiple DOX treatments at a therapeutic dose were assessed for their effect on the antioxidant enzyme status of cardiac and kidney tissue. DOX was administered i.p. (5 mg/kg) once a week for two weeks to male balb/c mice. The activities of the antioxidant enzymes superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPOX) and glutathione reductase (GR) were measured 1, 2 and 7 days following the second DOX treatment in both heart and kidney. Levels of reduced glutathione (GSH) were also measured in cardiac tissue at these same times. Cardiac levels of GPOX and GR showed a time-dependent decrease in activity, with 10% and 12% inhibition for GPOX and GR, respectively, at 7 days post second treatment. Cardiac levels of GSH also showed a significant decrease, approximately 15%, at 7 days post second treatment. Cardiac levels of SOD and CAT as well as kidney levels of all four antioxidant enzymes were not affected by DOX treatment. These data suggest that DOX given in a therapeutic regimen, at a therapeutic dose, can cause decreases in cardiac levels of GPOX, GR and GSH that could render the heart especially susceptible to further oxidative challenge.","PeriodicalId":12438,"journal":{"name":"Free radical research communications","volume":"19 2","pages":"111-20"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10715769309056505","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19214316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 40

Reaction kinetics of alpha-tocopheroxyl radical with biologically and pharmacologically active substances. α -生育酚自由基与生物和药理学活性物质的反应动力学。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309056495

K Ondrias, V Misík, V Brezová, A Stasko

引用次数: 6

Hydroxyl radical generation and membrane fluidity of erythrocytes treated with lipopolysaccharide. 脂多糖处理红细胞的羟基自由基生成和膜流动性。

Free radical research communications Pub Date : 1993-01-01 DOI: 10.3109/10715769309056s177

Y Hino, R Kumashiro, M Sata, J Nishi, R Ogura, K Tanikawa

{"title":"Hydroxyl radical generation and membrane fluidity of erythrocytes treated with lipopolysaccharide.","authors":"Y Hino, R Kumashiro, M Sata, J Nishi, R Ogura, K Tanikawa","doi":"10.3109/10715769309056s177","DOIUrl":"https://doi.org/10.3109/10715769309056s177","url":null,"abstract":"The effect of lipopolysaccharide (LPS) and/or bile acids on rat erythrocyte membranes was studied in vitro. Addition of LPS isolated from E. coli (J5 mutant) into the erythrocyte resulted in the decrease of membrane fluidity as determined by spin labelling using electron paramagnetic resonance (EPR). This was accompanied by membrane fragility. It was found that hydroxyl radicals were generated from erythrocytes treated with LPS by using DMPO spin trapping. However, pretreatment of erythrocytes with taurine-conjugated bile acids was found to modify the membrane response induced by LPS. Taurocholic acid (TCA) and tauroursodeoxycholic acid (TUDCA) prevented the decrease of membrane fluidity induced by LPS, and, as a result, the membrane integrity was maintained although no significant changes were observed in the amount of hydroxyl radicals produced by LPS addition. However, taurochenodeoxycholic acid (TCDCA) exhibited little beneficial effect on the dynamic properties and the function of the erythrocyte membranes, although the hydroxyl radical declined markedly in the erythrocytes. Therefore, it is suggested that TCA and TUDCA have a protective effect against LPS-induced membrane fragility by modulating membrane fluidity.","PeriodicalId":12438,"journal":{"name":"Free radical research communications","volume":"19 Suppl 1 ","pages":"S177-84"},"PeriodicalIF":0.0,"publicationDate":"1993-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/10715769309056s177","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19269695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6