Food ResearchPub Date : 2024-04-10DOI: 10.26656/fr.2017.8(2).241
U. Issara, N. Teerapattarakan
{"title":"Effects of oleogels (alpha-linolenic acid plus beeswax) extracted\u0000supplementation for approaching the therapeutic food ingredient: in vitro model","authors":"U. Issara, N. Teerapattarakan","doi":"10.26656/fr.2017.8(2).241","DOIUrl":"https://doi.org/10.26656/fr.2017.8(2).241","url":null,"abstract":"The dietary fatty acid intake for vascular disease has been highlighted for a decade. This\u0000study aimed to determine the effect of palmitic acid and fatty acid-incorporated beeswax\u0000(oleogels: OG) supplementation on anti-atherosclerotic effects through vascular smooth\u0000muscle cell (VSMC) activity. The A7r5 cells were cultured, cells were treated with three\u0000treatments, including 1) control, 2) palmitic acid (PA), and 3) alpha-linolenic acid (ALA)\u0000+ beeswax, ratio 1:1 (OG) at different concentrations of 0, 25, 50 and 100 μM for 48 hrs.\u0000Cell proliferation, cell apoptosis, wound repair, and relative quantity of mRNA level of\u0000inflammatory cytokines and angiogenetic transcription factors were determined. The\u0000results showed there was a significantly reduced VSMCs proliferation with concentrationdependent (p < 0.05). In the presence of OG at 100 μM, the wound area had healed after\u000024 hrs (59.2%) compared to PA (28.0%) group treatments. OG and PA supplementation\u0000significantly up-regulated eNOS and VEGF but down-regulated the TNF-α, CRP, CD36,\u0000iNOS, and NF-κB mRNA expression levels, while PA was a contrasting pattern (p <\u00000.05). Therefore, supplementation of OG reduced the pro-inflammatory effects of\u0000inflammatory cytokines in macrophages and induced VEGF expression in VSMCs,\u0000contributing to anti-atherosclerotic effect.","PeriodicalId":12410,"journal":{"name":"Food Research","volume":"598 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140719061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}