Effects of oleogels (alpha-linolenic acid plus beeswax) extracted supplementation for approaching the therapeutic food ingredient: in vitro model

Q3 Agricultural and Biological Sciences

Food Research Pub Date : 2024-04-10 DOI:10.26656/fr.2017.8(2).241

U. Issara, N. Teerapattarakan

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引用次数: 0

Abstract

The dietary fatty acid intake for vascular disease has been highlighted for a decade. This study aimed to determine the effect of palmitic acid and fatty acid-incorporated beeswax (oleogels: OG) supplementation on anti-atherosclerotic effects through vascular smooth muscle cell (VSMC) activity. The A7r5 cells were cultured, cells were treated with three treatments, including 1) control, 2) palmitic acid (PA), and 3) alpha-linolenic acid (ALA) + beeswax, ratio 1:1 (OG) at different concentrations of 0, 25, 50 and 100 μM for 48 hrs. Cell proliferation, cell apoptosis, wound repair, and relative quantity of mRNA level of inflammatory cytokines and angiogenetic transcription factors were determined. The results showed there was a significantly reduced VSMCs proliferation with concentrationdependent (p < 0.05). In the presence of OG at 100 μM, the wound area had healed after 24 hrs (59.2%) compared to PA (28.0%) group treatments. OG and PA supplementation significantly up-regulated eNOS and VEGF but down-regulated the TNF-α, CRP, CD36, iNOS, and NF-κB mRNA expression levels, while PA was a contrasting pattern (p < 0.05). Therefore, supplementation of OG reduced the pro-inflammatory effects of inflammatory cytokines in macrophages and induced VEGF expression in VSMCs, contributing to anti-atherosclerotic effect.

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油凝胶（α-亚麻酸加蜂蜡）提取物作为治疗食品成分的效果：体外模型

十年来，膳食脂肪酸摄入对血管疾病的影响一直备受关注。本研究旨在通过血管平滑肌细胞（VSMC）的活性，确定补充棕榈酸和脂肪酸融入蜂蜡（油凝胶：OG）对抗动脉粥样硬化作用的影响。以 A7r5 细胞为研究对象，分别用 1）对照组；2）棕榈酸（PA）；3）α-亚麻酸（ALA）+蜂蜡（OG），比例为 1:1，浓度分别为 0、25、50 和 100 μM，处理 48 小时，测定细胞增殖、细胞凋亡、创伤修复、炎症细胞因子和血管生成转录因子 mRNA 水平的相对数量。结果显示，VSMCs 的增殖明显减少，且与浓度有关（p < 0.05）。与 PA 组（28.0%）相比，在 100 μM 的 OG 存在下，伤口面积在 24 小时后愈合（59.2%）。补充 OG 和 PA 能显著上调 eNOS 和 VEGF，但下调 TNF-α、CRP、CD36、iNOS 和 NF-κB mRNA 表达水平，而 PA 则相反（p <0.05）。因此，补充 OG 可降低巨噬细胞中炎症细胞因子的促炎作用，并诱导血管内皮生长因子在血管内皮细胞中的表达，从而起到抗动脉粥样硬化的作用。

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来源期刊

Food Research Agricultural and Biological Sciences-Food Science

CiteScore

1.80

自引率

0.00%

发文量

285

审稿时长

12 weeks