Free Radicals and Antioxidants最新文献

{"title":"Resveratrol Nanoemulsion; A Promising Inhibitor against Mitogen-Activated Protein Kinase - Dependent Inflammation and Ameliorates Nicotine induced-lung Toxicity in Rats","authors":"Fedaa Talat Mohamed, Ammar Yasser Abdelghfour, Bassant Argawy Morsy, Doaa Diaa Rizk, Rania Mahmoud Elwany, Samiruzzaman Samir, A. A. Ali, Prof. Mohammed Abdalla Hussein","doi":"10.5530/FRA.2020.1.7","DOIUrl":"https://doi.org/10.5530/FRA.2020.1.7","url":null,"abstract":"Background: Nicotine, a major component of cigarette smoke, plays an important role in the development of cardiovascular disease and lung cancer in smokers. The aim of the present article was to investigate protective activity of resveratrol nanoemulsion (RENE) against lung toxicity induced by nicotine in adult rats as compared to basic resveratrol. Materials and Methods: RENE was prepared using bovine serum albumin method, then characterized for their particle size and zeta potential. Furthermore, Adult albino rats weighing around 150 ±10 g were used for the evaluation of lung protective activity of RENE (50 mg/k.b.w.) against nicotine-induced lung toxicity in rats. Results: The mean particle size of RENE was 49.5 ± 0.05 nm and zeta potential of +15.75 with the observed shapes of nanoparticle was spherical. The daily oral administration of the RENE at a concentration of 39.75 mg/kg body weight for 30 days to rats treated with nicotine (2.5 mg/kg.b.w.) resulted in a significant improve plasma cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol as well as serum tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6) and growth factor (TGF)-β1 in nicotine treated groups rats. On the other hand, oral administration of RENE elevated the activity of superoxide dismutase (SOD), glutathione peroxidase (GPx) and total protein kinase-1 (Akt-1) as well as reduced the level malondialdehyde (MDA) in lung rats treated with nicotine. In addition, RENE reduced the expression of lung inducible nitric oxide synthase (iNOS) and mitogen-activated protein kinase (p38-MAPK) levels as compared to nicotine treated control group. Also, RENE and resveratrol almost normalized these effects in the histoarchitecture of the lung. Conclusion: The obtained biochemical, molecular biology and histological results of our study proved the lung protective activity of RENE against nicotine induced lung toxicity in rats.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"40 1","pages":"35-41"},"PeriodicalIF":0.0,"publicationDate":"2020-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80381824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Hepatoprotective Effect of Vanillin in Isoniazid-Rifampicin Induced Hepatocellular Damage","authors":"R. Tiwari, A. Chourasia, A. Chaturvedi, A. Ganeshpurkar, Nazneen Dubey","doi":"10.5530/fra.2020.1.8","DOIUrl":"https://doi.org/10.5530/fra.2020.1.8","url":null,"abstract":"Cite this article: Tiwari R, Chourasia A, Chaturvedi A, Ganeshpurkar A, Dubey N. Evaluation of Hepatoprotective Effect of Vanillin in Isoniazid-Rifampicin Induced Hepatocellular Damage. Free Radicals and Antioxidants. 2020;10(1):42-6. ABSTRACT Objectives: Natural products are greatly acknowledged for antioxidant and hepatoprotective effects. Vanillin has been studied for radical scavenging effect. The aim of this study was to examine hepatoprotective effect of vanillin against isoniazid and rifampicin induced liver damage in rats. Methods: Wistar rats were used in present study. All the animals study protocols were duly approved by Institutional Animal Ethics Committee of the Institute. Hepatotoxicity was induced by administration of against isoniazid (50 mg/kg) and rifampicin (100 mg/kg) for 14 days. Vanillin was used in the dose of 50, 100 and 200 mg/kg body weight. At the end of study blood was collected and biochemical studies were performed to assess antioxidant status. Results: Oral administration of vanillin at test doses (50, 100 and 200 mg/ kg body weight) resulted in restoration of AST, ALT and ALP. There was a notable decrease in production of SOD and catalase. Conclusion: In the present study, vanillin demonstrated a notable hepatoprotective effect. The protective efficacy of vanillin is possibly because of radical scavenging and antioxidant property.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84622671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant and Antidiabetic Activities of Methanolic extract of Bark of Cinnamomum zeylanicum in Diabetic Rats","authors":"Rasvin Singh, S. Parasuraman, S. Kathiresan","doi":"10.5530/fra.2020.1.4","DOIUrl":"https://doi.org/10.5530/fra.2020.1.4","url":null,"abstract":"Background: Cinnamomum zeylanicum or Ceylon cinnamon is used for the management of dyspepsia, dysmenorrhea, memory loss and tremor. It is also traditionally recommended for the management of diabetes. Further studies are required to explore the antioxidant and antidiabetic activity of various extracts of bark of C. zeylanicum. Hence, the present study is planned to investigate the antioxidant and antidiabetic activities of methanolic extract of barks of C. zeylanicum. Methods: Bark of C. zeylanicum was extracted with methanol, ethanol and acetone and its antioxidant activity was studied using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging assays. Acute toxic effect of methanolic extract of C. zeylanicum (MECZ) studied as per the method described in OECD Guideline for testing of chemicals (Test Guideline 423). MECZ was studied for its antidiabetic effect using streptozotocin-induced diabetic rats. Results: In both DPPH and ABTS free radical scavenging assay, methanolic and acetone extracts exhibited free radical scavenging activity, respectively. In acute toxicity testing, MECZ did not show any significant toxic signs up to 2000 mg/kg, hence the antidiabetic activity of MECZ was carried out at the dose levels of 125, 250 and 500 mg/kg. MECZ showed antidiabetic activity from 2nd week of the experiment onward. In the biochemical analysis, MECZ treated animals showed significant decreases in the levels of ALP and urea when compared with control. In lipid profile analysis, diabetic animals and diabetic animals treated glibenclamide showed significant increases in the levels of total cholesterol when compared with normal control and MECZ prevented the STZ-induced hyperlipidemia. At the end of the study, diabetic animals and diabetic animals treated with glibenclamide and MECZ showed significant decreases in the level of insulin when compared with the control group. In the histopathological analysis, sections from the liver, pancreas and kidney of the diabetic animals showed mid-to-moderate toxic effects and glibenclamide and MECZ 500 mg/kg prevented the STZ-induced cellular changes. Conclusion: The MECZ exhibited significant antioxidant and antidiabetic activities.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"745 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74177570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Epigallocatechin Gallate on Cadmium Chloride-induced Oxidative Stress in Female Sprague Dawley Rats","authors":"S. Parasuraman, James Yu Kar Beng, L. Hui, Brenda Ngu Yen Qin","doi":"10.5530/fra.2020.1.6","DOIUrl":"https://doi.org/10.5530/fra.2020.1.6","url":null,"abstract":"Background: Epigallocatechin gallate (EGCG) is the ester of epigallocatechin and gallic acid. EGCG is abundant in dry tea leaves and its effect on heavy metal-induced oxidative stress is not clear. Hence, the present study is planned to study the effect of EGCG on cadmium chloride (CdCl2) induced oxidative stress in female Sprague Dawley rats. Methods: The rats were divided into six groups with each of six animals viz., control, CdCl2, vitamin C, EGCG, CdCl2 + vitamin C and CdCl2 + EGCG. CdCl2 (5 mg/kg) was suspended in carboxymethyl cellulose and administered orally to induce oxidative stress. Vitamin C and EGCG were dissolved in sterile water for injection and administered intraperitoneally within 15 min after CdCl2 administration. All the animals were administered with respective assigned treatment once daily for 28 consecutive days. At the end of the study, blood samples were collected from all the animals and serum was separated. The serum sample was used for biochemical analysis. Later, the rats were sacrificed and liver samples were collected and used for antioxidant assay. Results: EGCG and vitamin C prevented the CdCl2-induced oxidative stress. CdCl2 administered group showed significant increases in the levels of glucose, AST, ALT and urea when compared with control group, whereas vitamin C and EGCG prevented the CdCl2-induced biochemical changes. Vitamin C and EGCG also prevented the CdCl2-induced reduction in levels of reduced glutathione and catalase. Conclusion: EGCG had significant ameliorative effect on CdCl2 -induced oxidative stress in experimental animals.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"202 1","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2020-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91115825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of Relative Antioxidant Potential of Induced Variants of Catharanthus roseus (L.) G. Don in Salt Stress Condition for the Effective Selection of Salt Tolerant Variant","authors":"A. Verma","doi":"10.5530/fra.2019.2.13","DOIUrl":"https://doi.org/10.5530/fra.2019.2.13","url":null,"abstract":"Cite this article: Verma AK. Study of Relative Antioxidant Potential of Induced Variants of Catharanthus roseus (L.) G. Don in Salt Stress Condition for the Effective Selection of Salt Tolerant Variant. Free Radicals and Antioxidants. 2019;9(2):73-7. ABSTRACT Objectives: Catharanthus roseus (L.) G. Don is an important plant documented for variety of medicinal uses and salt tolerant potential. During genetic improvement programme an attempt of induced mutagenesis was made for isolation of improved genotype of C. roseus var. Nirmal (CIMAP 0865) and nine EMS induced variants were isolated. Present study was made for the assessment of relative antioxidative potential of these variants and identification/selection of salt tolerant genotype. Methods: For the assessment of relative antioxidative potential under glass house condition one month old seedlings of all variants exposed to salt stress condition and the activities of antioxidant enzymes viz., superoxide dismutase, peroxidase and catalase were estimated in leaf tissue at 4th day and 8th day of NaCl supply while proline and total alkaloid contents were estimated at 8th day of NaCl supply. Results: In general, NaCl imposition causes increase in activity of earlier two enzymes, decrease in catalase activity and stimulatory effect on proline and total alkaloid content. Values for these parameters varied with type of variants. Variants V2, V3 and V7 exhibited higher activity of antioxidant enzymes and had high accumulation of proline and total alkaloids. Conclusion: The variants V2, V3 and V7, having higher estimates for these parameters than that of parental variety which indicates their better survival/adaptive potential against salt stress condition and in context of salt tolerant may be utilized in genetic improvement programme of C. roseus.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"9 1","pages":"73-77"},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86118244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lifestyle Impact on Cognitive Decline and Neurodegeneration Overview groupview","authors":"A. David, S. Parasuraman","doi":"10.5530/fra.2019.2.10","DOIUrl":"https://doi.org/10.5530/fra.2019.2.10","url":null,"abstract":"Cite this article: David AVA, Parasuraman S. Lifestyle Impact on Cognitive Decline and Neurodegeneration Overview. Free Radicals and Antioxidants. 2019;9(2):52-7. ABSTRACT Cognitive decline is a part of a natural phenomenon that eventually happens to almost everyone as they age. It results from a combination of lifestyle habits, genetic factors, trauma or just gradual wearing-out of one’s nervous system. The effects of lifestyle and behavior which include patterns of dietary intake, physical activities as well as practicing unhealthy habits such as smoking and alcohol consumption, have been globally emphasized and embraced time and again as a crucial component to avoid serious health complications. It is also been included as an evidence-based guideline for children, adolescents and adults from diverse populations in all countries. Healthy lifestyle behaviors and therapeutic lifestyle changes were emphasized and reaffirmed as central to cardiovascular health promotion and risk reduction, respectively. Certain healthy behaviors known to combat cancer, cardiovascular disease, diabetes and may also reduce the risk of cognitive decline. Simple lifestyle changes might delay the start of cognitive decline and dementia or slow its progression. Activities that boost your body and brain are such as staying mentally active, engaging in regular physical activity and eating a heart-healthy diet. There are also evidences where people may benefit from staying socially engaged with friends, family and the community. People can reduce the risk of cognitive decline by making key lifestyle changes. There are measures which can be taken to modify their lifestyle while they still can before it’s too late to stop if not to minimize the onset of neuron degeneration.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82687484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Do Creatinine Blood Levels Act upon Fatty Acid Metabolites in Human Red Blood Cell Membranes?","authors":"Gallone Giuseppe, M. Guglielmo","doi":"10.5530/FRA.2019.2.14","DOIUrl":"https://doi.org/10.5530/FRA.2019.2.14","url":null,"abstract":"Cite this article: Giuseppe G, Guglielmo M. Do Creatinine Blood Levels Act upon Fatty Acid Metabolites in Human Red Blood Cell Membranes?. Free Radicals and Antioxidants. 2019;9(2):78-82. ABSTRACT Objectives: The aim of the present research is to correlate creatininemia and uricemia to HNE and MDA levels of Red Blood Cell purified membranes in comparison to those of normal subjects to characterize the effects of oxidative stress. Methods: The studied population consisted of 10 patients with endogenous both hypercreatininemic hyperuricemic and ten normal subjects. The measurement of total proteins in RBC ghosts and HNE and MDA are conducted on blood samples of patients. Results: The obtained data evidence that both creatinine and uric acid levels have influence on the ratio of both MDA/protein and HNE/protein content on RBC ghosts, demonstrating their possible protective role against oxidative stress at low concentrations in blood and oxidizing power at higher concentrations. Conclusion: The displayed results in this study suggest that at low concentrations of creatinine and uric acid there is oxidative stress in human erythrocyte membrane, but if creatinine and uric acid have slowed down to sufficient limits there is also oxidative stress, as demonstrated by MDA and HNE levels; only if the concentration of these two substances overwhelms a threshold.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"385 1","pages":"78-82"},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76444769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gallic Acid Radical Generation in Aromatic Plants: A Combined EPR and UV-Vis Spectroscopic Approach","authors":"E. Giannakopoulos, Georgios Salachas, A. Papasavvas, D. Hela","doi":"10.5530/fra.2019.2.15","DOIUrl":"https://doi.org/10.5530/fra.2019.2.15","url":null,"abstract":"1School of Science and Technology, Hellenic Open University, Patras, GREECE. 2Department of Agriculture, Laboratory of Plant Physiology and Biochemistry, University of Patra, Mesologhi, GREECE. 3Department Greenhouses Crops and Floriculture, Labortaroy of Plant Physiology, Technological Education Institute of Mesolonghi, Mesolonghi, GREECE. 4Department of Chemistry, University of Ioannina, Ioannina, GREECE.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"24 1","pages":"83-87"},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89833828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant, Anti-inflammatory, Antiproliferative and Antimicrobial Activities of Combretum glutinosum and Gardenia aqualla Extracts in vitro","authors":"B. Muhammad, N. Shaban, F. H. Elrashidy, D. Ghareeb","doi":"10.5530/fra.2019.2.12","DOIUrl":"https://doi.org/10.5530/fra.2019.2.12","url":null,"abstract":"66 Free Radicals and Antioxidants, Vol 9, Issue 2, Jul-Dec, 2019 Bawa Yusuf Muhammad1,2,*, Nadia Zaky Shaban1, Fatima Hassan Elrashidy1, Doaa Ahmed Ghareeb1,3 1Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria, EGYPT. 2Department of Biochemistry and Molecular Biology, Faculty of Science, Nasarawa State University Keffi, NIGERIA. 3Bioanalytical centres of Excellence: Pharmaceutical and fermentation Department, GEBRI, SRAT-city, Alexandria, EGYPT.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"1 1","pages":"66-72"},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83292224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Profile and Comparative Anti-radical Scavenging Activities of n-Hexane Extracts of Indigenous Zingiber officinale and Curcuma longa","authors":"C. Nweze, E. Dingwoke, F. Adamude, N. Nwobodo","doi":"10.5530/FRA.2019.2.11","DOIUrl":"https://doi.org/10.5530/FRA.2019.2.11","url":null,"abstract":"1Department of Biochemistry, Nasarawa State University, Keffi, Nasarawa, NIGERIA. 2Department of Biochemistry, Ahmadu Bello University, Zaria, Kaduna, NIGERIA. 3Department of Medical Biochemistry, Federal University, Lafia, Nasarawa, NIGERIA. 4Department of Pharmacology and Therapeutics, College of Medicine, Enugu State University of Science and Technology, Enugu, NIGERIA. 5Department of Pharmacology and Therapeutics, College of Health Sciences, Nile University of Nigeria, FCT, Abuja, NIGERIA.","PeriodicalId":12388,"journal":{"name":"Free Radicals and Antioxidants","volume":"18 1","pages":"58-65"},"PeriodicalIF":0.0,"publicationDate":"2020-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73236566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}