Forensic Toxicology最新文献_第10页

Fast and reliable profiling of cannabinoids in seized samples using the method of HPLC-DAD followed by chemometrics. 采用HPLC-DAD -化学计量学方法快速、可靠地分析检获样品中的大麻素。

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-07-01 DOI: 10.1007/s11419-022-00625-x

Sabrina Büttenbender, Graciela Carlos, Martin Steppe, Rafael Scorsatto Ortiz, Renata Pereira Limberger, Andreas Sebastian Loureiro Mendez

引用次数: 0

Quantitation of sibutramine in human hair using gas chromatography-isotope dilution tandem mass spectrometry. 气相色谱-同位素稀释串联质谱法定量人发中的西布曲明。

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-07-01 DOI: 10.1007/s11419-021-00609-3

Hongkun Yang, Amin Wurita, Jinlei Liu, Yue Wang, Koutaro Hasegawa

{"title":"Quantitation of sibutramine in human hair using gas chromatography-isotope dilution tandem mass spectrometry.","authors":"Hongkun Yang, Amin Wurita, Jinlei Liu, Yue Wang, Koutaro Hasegawa","doi":"10.1007/s11419-021-00609-3","DOIUrl":"https://doi.org/10.1007/s11419-021-00609-3","url":null,"abstract":"Purpose: An analytical method for quantitation of sibutramine in human hair using gas chromatography (GC)-isotope dilution tandem mass spectrometry (MS/MS) was newly established. In this article, a case is presented, in which a 3.5-year-old male child accidentally ingested chocolate-like product containing sibutramine, showing various symptoms; he could survived the crisis. About 1 month after the incident, his scalp hair sample was subjected to analysis for the causative sibutramine.Method: After cryo-grinding for the hair sample, target compound was extracted with methanol, and the solvent layer was evaporated to dryness. The residue was reconstituted in methanol and analyzed by GC-MS/MS, using the selected reaction monitoring (SRM) mode with a deuterated isotope internal standard.Results: The substance was identified as sibutramine; its concentration in the hair sample of the child was 58.6 pg/mg. The calibration curve of sibutramine in hair samples had a good linear relationship in the concentration range of 20-200 pg/mg (r > 0.99); the extraction recovery rate 85.2-91.8%; the interday and intraday precision and accuracy (bias) examined not greater than 9.6%. Sibutramine in human hair had good stability under 3 different storage conditions at room (20 °C), refrigerated (4 °C) and frozen ( - 20 °C) temperatures for at least 7 days.Conclusions: It should be expected that the method established in this study would contribute to rapid determinations of sibutramine. To our knowledge, this is the first report describing quantitation of sibutramine in an authentic human hair sample by GC-MS/MS.","PeriodicalId":12329,"journal":{"name":"Forensic Toxicology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10612049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phase I-metabolism studies of the synthetic cannabinoids PX-1 and PX-2 using three different in vitro models. 使用三种不同的体外模型对合成大麻素PX-1和PX-2进行i期代谢研究。

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-07-01 DOI: 10.1007/s11419-021-00606-6

Patrick Dahm, Andreas Thomas, Markus A Rothschild, Mario Thevis, Katja Mercer-Chalmers-Bender

{"title":"Phase I-metabolism studies of the synthetic cannabinoids PX-1 and PX-2 using three different in vitro models.","authors":"Patrick Dahm, Andreas Thomas, Markus A Rothschild, Mario Thevis, Katja Mercer-Chalmers-Bender","doi":"10.1007/s11419-021-00606-6","DOIUrl":"https://doi.org/10.1007/s11419-021-00606-6","url":null,"abstract":"Purpose: Synthetic cannabinoids (SCs), highly metabolized substances, are rarely found unmodified in urine samples. Urine screening relies on SC metabolite detection, requiring metabolism knowledge. Metabolism data can be acquired via in vitro assays, e.g., human hepatocytes, pooled human liver microsomes (pHLM), cytochrome P450 isoforms and a fungal model; or in vivo by screening, e.g., authentic human samples or rat urine. This work describes the comprehensive study of PX-1 and PX-2 in vitro metabolism using three in vitro models. 5F-APP-PICA (PX-1) and 5F-APP-PINACA (PX-2) were studied as they share structural similarity with AM-2201, THJ-2201 and 5F-AB-PINACA, the metabolism of which was described in the literature.Methods: For SC incubation, pHLM, cytochrome P450 isoenzymes and the fungal model Cunninghamella elegans LENDNER (C. elegans) were used. PX-1 and PX-2 in vitro metabolites were revealed comprehensively by liquid chromatography-high-resolution mass spectrometry measurements.Results: In total, 30 metabolites for PX 1 and 15 for PX-2 were detected. The main metabolites for PX-1 and PX-2 were the amide hydrolyzed metabolites, along with an indole monohydroxylated (for PX-1) and a defluorinated pentyl-monohydroxylated metabolite (for PX-2).Conclusions: CYP isoforms along with fungal incubation results were in good agreement to those obtained with pHLM incubation. CYP2E1 was responsible for many of the metabolic pathways; particularly for PX-1. This study shows that all three in vitro assays are suitable for predicting metabolic pathways of synthetic cannabinoids. To establish completeness of the PX-1 and PX-2 metabolic pathways, it is not only recommended but also necessary to use different assays.","PeriodicalId":12329,"journal":{"name":"Forensic Toxicology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9715525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9180141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Determination of abamectin in heart blood and urine following lethal intoxication: a case report. 致死性中毒后心脏血和尿中阿维菌素的测定:1例报告。

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-07-01 DOI: 10.1007/s11419-022-00617-x

Hao Dai, Daoxia Li, Li Xiao, Lin Yang, Songfan Li, Hong Yang, Yanshu Jiang, Yi Ye

引用次数: 0

Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago. 组织学石蜡包埋块:一个很好的替代标本检测使用阿片类药物至少20年前。

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-07-01 DOI: 10.1007/s11419-022-00614-0

Domenico Di Candia, Michele Boracchi, Guendalina Gentile, Gaia Giordano, Riccardo Zoja

{"title":"Histological paraffin-embedded block: a good alternative specimen to detect the use of opiates at least 20 years ago.","authors":"Domenico Di Candia, Michele Boracchi, Guendalina Gentile, Gaia Giordano, Riccardo Zoja","doi":"10.1007/s11419-022-00614-0","DOIUrl":"https://doi.org/10.1007/s11419-022-00614-0","url":null,"abstract":"Purpose: Since the solely certain remnants of a performed autopsy are formalin-fixed paraffin-embedded (FFPE) samples, stored in the archives of every institute of legal medicine, we managed to extract molecules of toxicological interest from these specimens.Methods: We assessed the analysis of ten fresh liver samples collected from heroin-related deaths and then histologically processed the same samples. The embedded blocks were then extracted by means of a new extracting method and the eluates were measured. We also selected five toxicological cases of heroin-related fatalities that were examined 20 years ago, collected the toxicological result documents of the analysis that were carried out at the time and then processed the corresponding FFPE liver samples that were stored in the archives.Results: We managed to isolate heroine-related metabolites from 20-year-old paraffin-embedded blocks and calculated ratios to evaluate the performance of our new extraction.Conclusions: According to our study, it is feasible to carry out a toxicological examination on old histological samples and, therefore, this matrix can be considered as a new alternative specimen for chemical-analytical evaluations of past cases or when fresh samples are not available anymore. The new extractive method was evaluated as efficient in treating these complex, paraffin-embedded samples. It was surprising that the target compounds could be quantitated from FFPE bocks created as long as 20 years ago.","PeriodicalId":12329,"journal":{"name":"Forensic Toxicology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9715498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9180145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Analytical Strategy 分析策略

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-01-01 DOI: 10.1016/b978-0-12-819286-3.00009-9

N. Lappas, Courtney M. Lappas

引用次数: 0

The Case of Claus von Bulow 克劳斯·冯·比洛案

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-01-01 DOI: 10.1016/b978-0-12-819286-3.00029-4

N. Lappas, Courtney M. Lappas

引用次数: 0

Glossary 术语表

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-01-01 DOI: 10.1016/b978-0-12-819286-3.17001-0

引用次数: 0

Sample Handling 样品处理

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-01-01 DOI: 10.1007/springerreference_67606

N. Lappas, Courtney M. Lappas

引用次数: 0

Quality Assurance and Quality Control 质量保证和质量控制

IF 2.2 4区医学

Forensic Toxicology Pub Date : 2022-01-01 DOI: 10.1016/B978-0-12-799967-8.00011-6

N. Lappas, Courtney M. Lappas

引用次数: 0