Experimental oncology最新文献

{"title":"DIFFERENTIAL EXPRESSION PATTERN OF AIP, UCKL1, AND PKN1 GENES IN PROSTATE CANCER PATIENTS.","authors":"L. Kovalevska, T. Zadvornyj, N. Lukianova, E. Kashuba","doi":"10.32471/exp-oncology.2312-8852.vol-44-no-1.17380","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-44-no-1.17380","url":null,"abstract":"BACKGROUND\u0000The evolution of research on the therapy of prostate cancer (PC) depends on a study of molecules that are involved in the progression of this disease. Nevertheless, there is a need for additional biomarkers that would help to refine the molecular profile of PC and propose the personalized therapeutic approach.\u0000\u0000\u0000AIM\u0000To study differential expression patterns of the AIP, UCKL1, and PKN1 genes in blood sera and tumor tissue of patients with PC with different Gleason scores.\u0000\u0000\u0000MATERIALS AND METHODS\u0000The total extracellular RNA was isolated from blood sera of 44 PC patients and 4 healthy donors. cDNAs were synthesized and quantitative polymerase chain reaction (qPCR) was performed. Immunohistochemical study of the UCKL, AIP and PKN1 proteins was performed on deparaffinized sections of tumors. The study was supplemented by a bioinformatic analysis of the publicly available databases.\u0000\u0000\u0000RESULTS\u0000The UCKL1, AIP, PKN1 genes were overexpressed at the mRNA level in blood sera of PC patients, compared to healthy donors. Extracellular mRNA levels of AIP and UCKL-1 were 100-1000-fold increased in all PC samples compared to the healthy donors but without significant inequality between the groups of PC cases differing by the Gleason score. The highest levels were detected in the samples from PC patients with the Gleason score > 9. The PKN1 expression was higher in PC patients compared with healthy donors but without significant difference between the groups.\u0000\u0000\u0000CONCLUSIONS\u0000From the three chosen genes, AIP and UCKL1 showed similar pattern of expression assessed either by extracellular mRNA levels in patient sera or the protein in PC tissues. AIP was up to 1000-fold increased in all PC samples, compared to the healthy donors, with the highest levels in PC cases with Gleason score > 9. Expression levels of the AIP and UCKL1 genes in the PC patient sera may be used as an additional criterion for prognosis of tumor progression.","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"44 1 1","pages":"47-51"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44914290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ON THE ORIGIN OF LUNG CANCER DEVELOPMENT.","authors":"L. Bolgova, T. Tuganova, O. I. Alekseenko, A. Ponomarenko","doi":"10.32471/exp-oncology.2312-8852.vol-44-no-1.17227","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-44-no-1.17227","url":null,"abstract":"In the review on the issues of histogenesis of lung cancer (LC), the significant results of experimental, pathological, immunohistochemical and molecular-biological studies are presented. However, until now, no data has been obtained that would allow scientists to come to an unambiguous decision about the origin and development of this disease. The question remains not yet fully understood, since researchers discuss the origin of LC development in a hypothetical form. This situation poses the task of further in-depth scientific research, which would make it possible to clarify the unambiguous origin of LC development, and, therefore, would allow solving the problem of its early diagnosis and create grounds for screening programs, which is of high scientific and clinical significance.","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"44 1 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41408267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An unusual presentation of primary cutaneous follicle center lymphoma.","authors":"A Janowska,&nbsp;C Fidanzi,&nbsp;G Granieri,&nbsp;M Iannone,&nbsp;A G Bonadio","doi":"10.32471/exp-oncology.2312-8852.vol-43-no-4.17026","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-4.17026","url":null,"abstract":"<p><p>Primary cutaneous follicle center lymphoma (PCFCL) is a rare low-grade cutaneous B-cell lymphoma. Clinically, PCFCL is usually an erythematous subcutaneous nodule or an infiltrated plaque. The dermoscopy is non-specific and it is characterized by polymorphous vascular pattern, arborizing vessels over a salmon-colored background and white areas. We reported a case of a 36-year-old woman presented with a rapidly growing, flashed-color, exophytic, soft consistency nodule on her scalp. Dermoscopy showed a diffuse structureless, skin-color area associated with a rare arborizing vascular pattern and brown circles. We reported a peculiar clinical and dermoscopic variant. This clinical presentation of PCFCL is unusual and represents a pitfall in the early clinical diagnosis. Histopathology is mandatory for a correct diagnosis.</p>","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"43 4","pages":"376-378"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39772944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High grade breast ductal carcinomas have high density of tumor-associated macrophages.","authors":"Ata Abbasi,&nbsp;Sepideh Rahimi,&nbsp;Leila Mahmoudzadeh,&nbsp;Hengameh Mojdeganlou","doi":"10.32471/exp-oncology.2312-8852.vol-43-no-4.16898","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-4.16898","url":null,"abstract":"<p><strong>Background: </strong>The role of tumor-associated macrophages (TAMs) is double-natured and still controversial. Depending on different settings, macrophages may suppress or promote tumor growth. TAM density may be one of the predictive factors of treatment outcome in cancer patients.</p><p><strong>Aim: </strong>To evaluate the density of tumor-associated macrophages in breast cancer and its relationship with various histopathologic findings.</p><p><strong>Materials and methods: </strong>55 patients with invasive ductal carcinoma of breast who underwent mastectomy were enrolled. Sections of tumor samples were stained and the density of CD68⁺ cells was evaluated.</p><p><strong>Results: </strong>There was an association between estrogen receptor (ER) expression and CD68 density (p = 0.010) as the higher densities of CD68 were seen in ER negative tumors. Moreover, there was a significant relationship between histological grade and CD68 density (p = 0.006).</p><p><strong>Conclusion: </strong>The higher TAM density is associated with higher tumor grade and negative ER expression in breast cancer tissues. These findings revealed that inflammation could have an important role in malignancies.</p>","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"43 4","pages":"328-330"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39861004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of obesity in rats on prostate histology and expression of leptin receptor, prolactin receptor, IL-6, and NF-κB.","authors":"D Herrera-Covarrubias,&nbsp;C A Pérez-León,&nbsp;C Fernández-Pomares,&nbsp;G A Coria-Avila,&nbsp;V Sánchez-Zavaleta,&nbsp;G E Aranda-Abreu,&nbsp;J Suárez-Medellín,&nbsp;F Rojas-Durán,&nbsp;M E Hernández","doi":"10.32471/exp-oncology.2312-8852.vol-43-no-4.16826","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-4.16826","url":null,"abstract":"<p><strong>Background: </strong>Hypercaloric intake can lead to obesity, which is a major risk factor associated with chronic subclinical inflammation and many types of cancer. It can increase the serum levels of leptin, prolactin, nuclear factor kappa B (NF-кB) and interleukin (IL)-6, implicated in cell proliferation, differentiation and survival.</p><p><strong>Aim: </strong>To explore the effects of obesity induced by chronic hypercaloric diet in rats on the long-term expression of leptin receptor (OB-R), prolactin receptor, NF-кB, and IL-6, and the changes of histology in rat prostate.</p><p><strong>Materials and methods: </strong>From postnatal day 21, experimental males were fed with normal chow or chow plus enriched hypercaloric liquid diet. On the postnatal day 90 (13 week old), the animals were euthanized for prostate histology (hematoxylin and eosin staining) and hormone receptors analysis by Western blot.</p><p><strong>Results: </strong>Hypercaloric diet resulted in obesity (32% higher body weight). The prostates of the obese males showed epithelium anisocytosis and compressed interstice. There was also greater volume of lipidic content, anisokaryosis, alterations of the nucleus-cytoplasm ratio, and apparent proplasia. Measures in the ventral prostate (VP) showed that alveoli area increased, but epithelium height and nucleus area were reduced. In the dorsolateral prostate, there was only reduction of nucleus area and presence of mononuclear cells in the lumen. Hypercaloric males also expressed a trend for more OB-R 130 kD in the VP, but no changes were observed with regard to prolactin receptor, NF-кB and IL-6.</p><p><strong>Conclusion: </strong>The obesity due to chronic consumption of hypercaloric diet affects both prostatic regions, but VP is possibly more sensitive via OB-R. We suggest that longer periods of obesity are needed to alter other receptors or the molecular markers of inflammation.</p>","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"43 4","pages":"317-321"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39861006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of ultrasound and MRI informativeness for detection and treatment monitoring of cervical cancer metastases in the vagina.","authors":"T S Golovko,&nbsp;O A Bakai,&nbsp;A V Ashykhmin,&nbsp;L M Baranovskaya","doi":"10.32471/exp-oncology.2312-8852.vol-43-no-4.16971","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-4.16971","url":null,"abstract":"<p><strong>Aim: </strong>To assess ultrasound (US) method informativeness in the recurrence detection and treatment monitoring of patients with cervical cancer metastases in the vagina via comparing the US and magnetic resonance imaging (MRI) data.</p><p><strong>Materials and methods: </strong>42 patients with recurrence of cervical cancer were examined by transvaginal US and MRI. Data on radiation diagnostics of cervical cancer metastasis in the vagina were compared with pathomorphological data. US and MRI data on the metastatic tumors size were compared.</p><p><strong>Results: </strong>The diagnostic efficiency of US and MRI for recurrence detection was determined. The sensitivity, specificity and accuracy of US were 92.8; 93.3 and 93.3%, respectively, and of MRI - 95.2; 96.6 and 95.8%, respectively. The informativeness of MRI was higher than US in cervical cancer recurrences detection, but the difference was not significant (p > 0.05). US can provide data as informative as MRI for estimation of metastases sizes in the vagina. The mean difference between MRI measurements and US measurements of the metastases volume was 0.79 mm (95% CI 0.62-0.95 cm³).</p><p><strong>Conclusions: </strong>US as a cheaper and simpler method could be an alternative for metastases detecting and treatment monitoring, especially if there are contraindications to MRI.</p>","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"43 4","pages":"351-358"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39861001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The giant phyllodes tumor of the breast - a case report.","authors":"V V Zaichuk,&nbsp;A A Samusieva,&nbsp;M V Diatel,&nbsp;M M Plodiienko","doi":"10.32471/exp-oncology.2312-8852.vol-43-no-4.16902","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-4.16902","url":null,"abstract":"<p><p>Phyllodes tumors are rare tumors of the breast, which are often misdiagnosed as fibroadenomas and difficult to treat with adjuvant therapy. Here, we present a case of a female patient with giant phyllodes tumor of the breast.</p>","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"43 4","pages":"373-375"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39861003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SLAMF1/CD150 expression and topology in prostate and breast cancer cell lines.","authors":"I M Gordiienko,&nbsp;O O Lykhova,&nbsp;V M Shcherbina,&nbsp;L M Shlapatska","doi":"10.32471/exp-oncology.2312-8852.vol-43-no-4.17010","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-4.17010","url":null,"abstract":"<p><strong>Background: </strong>SLAMF1/CD150 receptor is aberrantly expressed in malignant hematopoietic cells compared to ubiquitous expression in their normal analogues. However, the data about CD150 expression and function outside the hematopoietic system are limited. The aim of this pilot study was to examine the profile of mRNA expression of CD150 isoforms and the protein topology in highly and low malignant breast (BC) and prostate cancer (PC) cell lines.</p><p><strong>Materials and methods: </strong>The study was performed on BC T47D, MDA-MB-231, ВСС/Р and BC/ML cell lines and PC LNCap, Du-145 and PC-3 cell lines. The quantitative polymerase chain reaction was applied for study of CD150 isoforms mRNA expression and flow cytometry was used for determination of protein localization.</p><p><strong>Results: </strong>Analyzed BC cell lines did not express CD150 on the cell surface membrane (csCD150^-), but more than 45% of cells were positive for CD150 cytoplasmic reaction (cyCD150⁺). The cyCD150 expression level in T47D cells of luminal BC subtype was higher than in basal BC cell lines MDA-MB-231, ВСС/Р and BC/ML. The PC cell lines expressed CD150 both on the cell surface and in cytoplasm. The highest number of csCD150⁺ and cyCD150⁺ cells was revealed in less aggressive androgen responsive, non-metastatic LNCap cell line. All studied BC and PC cell lines expressed mRNA of canonical transmembrane mCD150 and novel nCD150 isoforms but with different pattern of prevalence. Soluble CD150 isoform was revealed at the low level only in BCC/P BC cell line and LNCap, PC-3 PC cell lines.</p><p><strong>Conclusions: </strong>We have shown that BC and PC cell lines differentially expressed multifunctional receptor CD150 at the mRNA and protein levels that may indicate its association with the degree of their malignancy.</p>","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"43 4","pages":"312-316"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39772945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative characteristics of DNA loop domains rearrangement in glioblastoma multiforme T98G and glioblastoma astrocytoma U373 cell lines under different culture conditions.","authors":"K Svyrydova,&nbsp;V Vasylieva,&nbsp;S Kropyvko,&nbsp;K Afanasieva,&nbsp;A Sivolob","doi":"10.32471/exp-oncology.2312-8852.vol-43-no-4.17080","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-4.17080","url":null,"abstract":"<p><strong>Background: </strong>The loop domain organization of chromatin, which plays an important role in transcription regulation, may depend on the cell functional state. The aim of this work was to investigate DNA loop reorganization upon functional transitions in two cell lines ‒ glioblastoma multiforme T98G and glioblastoma astrocytoma U373.</p><p><strong>Materials and methods: </strong>Single cell gel electrophoresis (the comet assay) was used to analyze the kinetics of the DNA loop migration from the nucleoids obtained from the lysed cells.</p><p><strong>Results: </strong>The DNA fraction in the surface loops and the size of these loops were found to be similar in two glioblastoma cell lines. When synthetic processes were inhibited, the migration of a small portion of inner loops was observed in T98G but not U373 cells. In T98G cells, stimulation of cell proliferation and transcription was accompanied by an increase in DNA fraction in the inner loops and an essential increase in the size of these loops. The effect of stimulation was practically absent in U373 cells. However, the linear density of the loops resolved by the comet assay was found to decrease upon stimulation of proliferation in both cell lines.</p><p><strong>Conclusion: </strong>A decrease in the loop density appears to be associated with an intensification of the synthetic processes in cells upon their stimulation.</p>","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"43 4","pages":"306-311"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39772539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distal sacrectomy for recurrent rectal cancer.","authors":"V V Kernychnyi,&nbsp;B E Li","doi":"10.32471/exp-oncology.2312-8852.vol-43-no-4.16794","DOIUrl":"https://doi.org/10.32471/exp-oncology.2312-8852.vol-43-no-4.16794","url":null,"abstract":"<p><p>Surgical treatment of recurrent rectal tumors is a challenge for cancer surgeons. Due to the high risk of intraoperative and postoperative complications, such tumors are often considered inoperable. We present a clinical case of 56-years-old male patient with local recurrence and pathological fracture of the sacrum at S5 level to whom en-bloc distal sacrectomy was performed. Such surgical technique for recurrence of rectal cancer provides significant improvement in local and general condition and creates a favorable prognosis for the patient's survival, which is demonstrated by the described clinical case.</p>","PeriodicalId":12287,"journal":{"name":"Experimental oncology","volume":"43 4","pages":"370-372"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39861008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}