Folia Biologica最新文献_第8页

Limonitum Ameliorates Castor Oil-Induced Diarrhoea in Mice by Modulating Gut Microbiota. 枸橼酸通过调节肠道菌群改善蓖麻油诱导的小鼠腹泻。

IF 1.1 4区医学

Folia Biologica Pub Date : 2022-01-01 DOI: 10.14712/fb2022068040133

Y L Ma, B F Yan, J Liu, S L Dai, J Liu, X X Wang, F Fang, S C Wu, Y Wang, C Y Xu, Q Zhao, H B Wang, D K Wu

{"title":"Limonitum Ameliorates Castor Oil-Induced Diarrhoea in Mice by Modulating Gut Microbiota.","authors":"Y L Ma, B F Yan, J Liu, S L Dai, J Liu, X X Wang, F Fang, S C Wu, Y Wang, C Y Xu, Q Zhao, H B Wang, D K Wu","doi":"10.14712/fb2022068040133","DOIUrl":"10.14712/fb2022068040133","url":null,"abstract":"Diarrhoea is a common clinical condition; its pathogenesis is strongly associated with gut microbiota dysbiosis. Limonitum is a well-known traditional Chinese medicine that exerts appreciable benefits regarding the amelioration of diarrhoea. However, the mechanism through which Limonitum ameliorates diarrhoea remains unclear. Here, the efficacy and underlying mechanism of Limonitum decoction (LD) regarding diarrhoea were explored from the aspect of gut microbiota. Castor oil (CO) was used to induce diarrhoea in mice, which were then used to evaluate the effects of LD regarding the timing of the first defecation, diarrhoea stool rate, degree of diarrhoea, diarrhoea score, intestinal propulsive rate, and weight of intestinal contents. The concentrations of short-chain fatty acids (SCFAs), including acetic, propionic, isobutyric, butyric and valeric acids, were analysed by gas chromatography-mass spectrometry (GC-MS). The 16S rRNA high-throughput sequencing technology was applied to evaluate changes in the gut microbiota under exposure to LD. LD was found to effectively ameliorate the symptoms of diarrhoea, and the diversity and relative abundance of gut microbiota were restored to normal levels following LD treatment. Additionally, LD significantly restored the observed reductions in SCFAs. These results provide strong evidence that LD can sufficiently ameliorate diarrhoea in mice by regulating their gut microbiota. The findings presented here highlight that Limonitum may constitute a prospective remedy for diarrhoea.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"68 4","pages":"133-141"},"PeriodicalIF":1.1,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10847151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circulating Cell-Free DNA Extraction from Liquid Biopsy for Cancer Research. 液体活检循环无细胞DNA提取用于癌症研究。

IF 1.1 4区医学

Folia Biologica Pub Date : 2022-01-01 DOI: 10.14712/fb2022068040153

L Pfeiferova, M Safarikova, J Ulrych, Z Krska, V Frankova, T Zima, M Kalousova

引用次数: 0

PLAG1 Promotes High Glucose-Induced Angiogenesis and Migration of Retinal Endothelial Cells by Regulating the Wnt/β-Catenin Signalling Pathway. PLAG1通过调节Wnt/β-Catenin信号通路促进高糖诱导的视网膜内皮细胞血管生成和迁移。

IF 0.6 4区医学

Folia Biologica Pub Date : 2022-01-01

Q Gu, H-F Wei

{"title":"PLAG1 Promotes High Glucose-Induced Angiogenesis and Migration of Retinal Endothelial Cells by Regulating the Wnt/β-Catenin Signalling Pathway.","authors":"Q Gu, H-F Wei","doi":"","DOIUrl":"","url":null,"abstract":"Proliferation and migration of retinal endothelial cells (RECs) contribute to the development of diabetic retinopathy. PLAG1 (pleomorphic adenoma gene 1) functions as a zinc-finger transcription factor to participate in the development of lipoblastomas or pleomorphic adenomas of the salivary glands through regulation of cell proliferation and migration. The role of PLAG1 in diabetic retinopathy was investigated in this study. Firstly, RECs were induced under high glucose conditions, which caused reduction in viability and induction of apoptosis in the RECs. Indeed, PLAG1 was elevated in high glucosetreated RECs. Functional assays showed that silence of PLAG1 increased viability and suppressed apoptosis in high glucose-induced RECs, accompanied with up-regulation of Bcl-2 and down-regulation of Bax and cleaved caspase-3. Moreover, migration of RECs was promoted by high glucose conditions, while repressed by knockdown of PLAG1. High glucose also triggered angiogenesis of RECs through up-regulation of vascular endothelial growth factor (VEGF). However, interference of PLAG1 reduced VEGF expression to retard the angiogenesis. Silence of PLAG1 also attenuated high glucose-induced up-regulation of Wnt3a, β-catenin and c-Myc in RECs. Moreover, silence of PLAG1 ameliorated histopathological changes in the retina of STZ-induced diabetic rats through down-regulation of β-catenin. In conclusion, knockdown of PLAG1 suppressed high glucose-induced angiogenesis and migration of RECs, and attenuated diabetic retinopathy by inactivation of Wnt/ β-catenin signalling.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"68 1","pages":"25-32"},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33489823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra-Small Gold Nanoparticles with Mild Immunomodulatory Activity as a Potential Tool for Bio-Applications. 具有温和免疫调节活性的超小金纳米颗粒作为生物应用的潜在工具。

IF 1.1 4区医学

Folia Biologica Pub Date : 2022-01-01 DOI: 10.14712/fb2022068040142

T Bělinová, P Javorová, H Y Nguyenová, A Řezníčková, Z Humlová, M Hubálek Kalbáčová

引用次数: 0

Time and Temperature Stability of TGF-β1, EGF and IGF-1 in 20% and 100% Human Serum. TGF-β1、EGF和IGF-1在20%和100%人血清中的时间和温度稳定性。

IF 0.6 4区医学

Folia Biologica Pub Date : 2022-01-01

K Jirsova, K Levova, M Kalousova, I Fales, V Frankova, V Vesela, T Zima, T P Utheim, J Bednar

{"title":"Time and Temperature Stability of TGF-β1, EGF and IGF-1 in 20% and 100% Human Serum.","authors":"K Jirsova, K Levova, M Kalousova, I Fales, V Frankova, V Vesela, T Zima, T P Utheim, J Bednar","doi":"","DOIUrl":"","url":null,"abstract":"Autologous serum eye drops (ASEDs) are used as a treatment for severe dry eye disease. The concentration and stability of various growth factors in ASEDs is determinative for their efficiency. We therefore assessed the concentrations of transforming growth factor beta 1 (TGF-β1), epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) in ASEDs following storage at 4-8, -20, -80 and -156 °C. Twenty % and 100% sera from eight healthy volunteers were analysed by the sandwich enzyme immunoassay at different time intervals up to seven months. The mean levels of TGF-β1 and EGF in undiluted and 20% serum did not differ significantly from the baseline levels in fresh serum for any storage conditions after 7 days at 4-8 °C, as well as after 4- and 7-month preservation at sub-zero temperatures. In 20% serum, no IGF-1 concentration decrease was found following 7 days of preservation at 4-8 °C. However, a decrease to 78 % and 81 % (P < 0.01) of baseline values was found in 20% serum after 4-month storage at -20 °C and 7-month storage at -156 °C, respectively. A more pronounced decrease in IGF-1 was observed in undiluted serum. All assessed growth factors present in 20% frozen serum remained stable for up to 7 months. The highest stability was achieved at -80 °C. At -20 and -156 °C, some decrease in IGF-1 occurred. Our results indicate that 20% ASEDs can be stored frozen up to 7 months under proper conditions.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"68 2","pages":"45-49"},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40687849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Morphology of Cell Differentiation, Terminal Differentiation and Ageing Seems To Reflect the Same Process: a Short Note. 细胞分化、终末分化和衰老的形态学似乎反映了同一个过程。

IF 0.6 4区医学

Folia Biologica Pub Date : 2021-01-01

K Smetana, D Mikulenkova, H Klamova

{"title":"The Morphology of Cell Differentiation, Terminal Differentiation and Ageing Seems To Reflect the Same Process: a Short Note.","authors":"K Smetana, D Mikulenkova, H Klamova","doi":"","DOIUrl":"","url":null,"abstract":"Based on simple microscopic cell morphology in blood and bone marrow smear preparations, it seems to be likely that the cell differentiation and terminal differentiation in human blood cells, and particularly in erythroid or granulocytic lineages, simultaneously reflect ageing of the lineage progenitors and terminal differentiation steps. The terminal differentiation stages of both these lineages actually appear as senescent cells. Abnormal ageing of progenitor cells may represent one of the \"dysplastic\" phenomena of the premature terminal differentiation state. Such state is characterized by heterochromatin condensation and nucleolar morphology similar to that in fully differentiated terminal cells of granulocytic or erythroid lineages. It should also be mentioned that in some known erythropoietic disorders, less differentiated erythroblasts may lose nuclei similarly as \"normal\" fully terminally differentiated cells of the erythroid cell lineage. It seems to be clear that cells in both abnormal less differentiated and terminally differentiated stages of erythroid or granulocytic lineages lose the ability to multiply similarly as senescent cells. On the other hand, the background of cell ageing and differentiation is very complicated and requires a different approach than the simple microscopic morphology at the single cell level. However, the morphology and clinical cytology at the single cell level might still contribute with complementary data to more sophisticated complex studies of that topic. In addition, the morphological approach facilitates the study of the main components of single cells in various states, including the differentiation steps or ageing.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"67 2","pages":"70-75"},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39499678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Latent Defect in Haematopoiesis of UBC-GFP Mice Sheds Light on the Lymphoid Developmental Potential of Haematopoietic Stem Cells. UBC-GFP小鼠造血潜在缺陷揭示造血干细胞的淋巴发育潜能

IF 1.1 4区医学

Folia Biologica Pub Date : 2021-01-01 DOI: 10.14712/fb2021067040135

E Nečas, K Faltusová, C-L Chen

引用次数: 0

Human Adrenocortical Carcinoma (NCI-H295R) Cell Line as an In Vitro Cell Culture Model for Assessing the Impact of Iron on Steroidogenesis. 人肾上腺皮质癌(NCI-H295R)细胞系作为体外细胞培养模型评估铁对甾体生成的影响。

IF 0.6 4区医学

Folia Biologica Pub Date : 2021-01-01

Z Knazicka, V Fialkova, H Duranova, J Bilcikova, E Kovacikova, M Miskeje, V Valkova, Z Forgacs, S Roychoudhury, P Massanyi, N Lukac

{"title":"Human Adrenocortical Carcinoma (NCI-H295R) Cell Line as an In Vitro Cell Culture Model for Assessing the Impact of Iron on Steroidogenesis.","authors":"Z Knazicka, V Fialkova, H Duranova, J Bilcikova, E Kovacikova, M Miskeje, V Valkova, Z Forgacs, S Roychoudhury, P Massanyi, N Lukac","doi":"","DOIUrl":"","url":null,"abstract":"The aim of this in vitro study was to examine the dose-dependent effects of iron as a potential endocrine disruptor in relation to the release of sexual steroid hormones by a human adrenocortical carcinoma (NCI-H295R) cell line. The cells were exposed to different concentrations (3.90, 62.50, 250, 500, 1000 μM) of FeSO4.7H2O and compared with the control group (culture medium without FeSO4.7H2O). Cell viability was measured by the metabolic activity assay. Quantification of sexual steroid production was performed by enzyme-linked immunosorbent assay. Following 48 h culture of the cells in the presence of FeSO4.7H2O, significantly (P < 0.001) increased production of progesterone was observed at the lowest concentration (3.90 μM) of FeSO4.7H2O, whereas the lowest release of progesterone by NCIH295R cells was noted after addition of 1000 μM of FeSO4.7H2O, which did not elicit cytotoxic action (P > 0.05). Testosterone production was substantially increased at the concentrations ≤ 62.50 μM of FeSO4.7H2O. Lower levels of testosterone were recorded in the groups with higher concentrations (≥ 250 μM) of FeSO4.7H2O (P > 0.05). The presented data suggest that iron has no endocrine disruptive effect on the release of sexual steroid hormones, but its toxicity may be reflected at other points of the steroidogenesis pathway.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"67 2","pages":"76-81"},"PeriodicalIF":0.6,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39498135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

T-Cell Activation: Post-Infection Diagnostic Tool for COVID-19. t细胞活化：COVID-19感染后诊断工具

IF 1.1 4区医学

Folia Biologica Pub Date : 2021-01-01 DOI: 10.14712/fb2021067010016

P Simara, L Tesarova, I Tapuchova, J Celerova, I Koutna

引用次数: 0

Acylated Ghrelin Administration Inhibits Sleeve Gastrectomy-Induced Hippocampal Oxidative Stress, Apoptosis and Tau-Hyperphosphorylation by Activating the PI3K/Akt Pathway. 乙酰化Ghrelin通过激活PI3K/Akt通路抑制袖胃切除术诱导的海马氧化应激、凋亡和tau过度磷酸化

IF 1.1 4区医学

Folia Biologica Pub Date : 2021-01-01 DOI: 10.14712/fb2021067020049

M D Morsy, M A Alsaleem, M S Aboonq, S O Bashir, H A Al-Daher

{"title":"Acylated Ghrelin Administration Inhibits Sleeve Gastrectomy-Induced Hippocampal Oxidative Stress, Apoptosis and Tau-Hyperphosphorylation by Activating the PI3K/Akt Pathway.","authors":"M D Morsy, M A Alsaleem, M S Aboonq, S O Bashir, H A Al-Daher","doi":"10.14712/fb2021067020049","DOIUrl":"10.14712/fb2021067020049","url":null,"abstract":"This study investigated the impact of exogenous replacement therapy with acylated ghrelin (AG) post sleeve gastrectomy (SG) on the memory function in rats. In addition, we investigated the possible underlying mechanisms, including the effects on markers of oxidative stress, tau phosphorylation, and apoptosis. Adult male Wistar rats were divided into four groups (N = 18/group) as follows: sham (control), SG, SG+AG (100 μM), and SG+AG+LY294002 (0.25 μg/100 g). We continued all treatments daily for four weeks post-surgery. SG impaired the spatial, retention, and recognition memories as tested by the Morris water maze test, passive avoidance test, and novel object recognition test, respectively. Also, it enhanced the levels of reactive oxygen species and lipid peroxides, reduced glutathione and protein levels of Bcl-2, and increased the levels of Bax and cleaved caspase-3 in the hippocampus. In addition, SG reduced the hippocampal levels of acetylcholine and brain-derived neurotrophic factor. Concomitantly, it inhibited the hippocampal activity of Akt and increased the activity of glycogen synthase kinase 3β and tau protein phosphorylation. Exogenous administration of acylated ghrelin to rats that had undergone SG prevented memory deficits. Also, it prevented the alteration in the above-mentioned biochemical parameters, an effect that was abolished by co-administration of LY294002 (phosphoinositide 3-kinase inhibitor). In conclusion, AG replacement therapy after SG in rats protects them against memory deficits and hippocampal damage by suppressing tau protein phosphorylation, mediated by activating PI3K/Aktinduced inhibition of glycogen synthase kinase 3β.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"67 2","pages":"49-61"},"PeriodicalIF":1.1,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39499676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0