Folia Biologica最新文献_第4页

Astaxanthin Induces Apoptosis in Human Osteosarcoma MG-63 Cells. 虾青素诱导人骨肉瘤 MG-63 细胞凋亡

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069050186

Guangyu Wang, Xu Tian, Lintao Liu, Jingming Dong

{"title":"Astaxanthin Induces Apoptosis in Human Osteosarcoma MG-63 Cells.","authors":"Guangyu Wang, Xu Tian, Lintao Liu, Jingming Dong","doi":"10.14712/fb2023069050186","DOIUrl":"https://doi.org/10.14712/fb2023069050186","url":null,"abstract":"We explored the mechanism of human osteosarcoma MG-63 cell apoptosis induced by asta-xanthin. The MTT assay was used to detect the effect of astaxanthin on cell viability. Morphological changes associated with apoptosis were observed after DAPI staining. Early and late stages of apoptosis were detected by flow cytometry with annexin V-FITC/PI staining. Activation of caspases-8, -9 and -3 was detected by enzyme activity in vitro. Changes in the mitochondrial membrane potential were detected by MitoCapture staining. Western blot was used to detect the cleavage of PARP, which is a caspase-3 substrate, the release of cytochrome c and Smac into the cytosol, the translocation of pro-apoptotic proteins Bax and Bak, and the expression of mitochondrial pathway-related proteins. The translocation of Bax was also detected by immunofluorescence assay. Astaxanthin significantly inhibited the viability of human osteosarcoma MG-63 cells with an IC50 value of 12.36 μg/ml. The DAPI-stained cells showed characteristic apoptotic morphological changes - cell shrinkage, cell membrane blebbing, nuclear condensation, and apoptotic body formation. Cytochrome c and Smac were released from mitochondria to the cytosol. Pro-apoptotic proteins Bax and Bak were rapidly translocated to mitochondria after six hours of astaxanthin action. Caspases-9 and -3 were activated and PARP was cleaved. The expression of anti-apoptotic proteins Bcl-2, Bcl-xL and XIAP was significantly decreased. Astaxanthin induced human osteosarcoma MG-63 cell apoptosis through the mitochondria-mediated endogenous apoptosis pathway.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 5-6","pages":"186-193"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autotaxin and Lysophosphatidic Acid Signalling: the Pleiotropic Regulatory Network in Cancer. Autotaxin 和溶血磷脂酸信号：癌症中的多向调控网络

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069050149

Ondřej Vít, Jiří Petrák

引用次数: 0

Many Ways to the Cell Cycle Exit after Inhibition of CDK4/6. CDK4/6 抑制后细胞周期退出的多种途径

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069050194

Libor Macůrek

引用次数: 0

Study of the Methylation of Bovine GSTP1 Gene under the Influence of Pesticide Mospilan 20SP Alone and in Combination with Pesticide Orius 25EW. 农药Mospilan 20SP单独及与农药Orius 25EW联合作用下牛GSTP1基因甲基化的研究

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069010006

Jana Halušková, Beáta Holečková, Lenka Luptáková, Ján Košuth, Viera Schwarzbacherová, Martina Galdíková, Simona Koleničová

{"title":"Study of the Methylation of Bovine GSTP1 Gene under the Influence of Pesticide Mospilan 20SP Alone and in Combination with Pesticide Orius 25EW.","authors":"Jana Halušková, Beáta Holečková, Lenka Luptáková, Ján Košuth, Viera Schwarzbacherová, Martina Galdíková, Simona Koleničová","doi":"10.14712/fb2023069010006","DOIUrl":"10.14712/fb2023069010006","url":null,"abstract":"DNA methylation, one of the most studied epigenetic mechanisms, when present in the promoter region of genes, causes inhibition of gene expression, and conversely, hypomethylation of these regions enables gene expression. DNA methylation is susceptible to nutritional and environmental influences, and undesirable alterations in methylation patterns manifested in changes in the expression of relevant genes can lead to pathological consequences. In the present work, we studied the methylation status of the bovine GSTP1 gene under the influence of pesticide Mospilan 20SP alone and in combination with pesticide Orius 25EW in in vitro proliferating bovine lymphocytes. We employed methylation-specific PCR, and when studying the effect of pesticide combinations, we also used its real-time version followed by a melting procedure. Our results showed that Mospilan 20SP alone at 5, 25, 50, and 100 µg.ml-1 and 5, 10, 25, and 50 µg.ml-1 for the last 4 and 24 hours of culture with in vitro proliferating bovine lymphocytes, respectively, did not induce methylation of the bovine GSTP1 gene. The same results were revealed when studying the effect of the combination of the pesticides added to the lymphocyte cultures for the last 24 hours of cultivation in the following amounts: 1.25, 2.5, 5, 10, and 25 µg.ml-1 of Mospilan 20SP and 1.5, 3, 6, 15, and 30 µg.ml-1 of Orius 25EW. We have also revealed that the less laborious real-time MSP followed by a melting procedure may replace MSP for studying the methylation status of the GSTP1 gene.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 1","pages":"6-12"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92153420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homeobox Protein PROX1 Expression is Negatively Regulated by Histone Deacetylase 1 and c-JUN Complex in MDA-MB-231 Human Breast Cancer Cells. 组蛋白去乙酰化酶 1 和 c-JUN 复合物对 MDA-MB-231 人乳腺癌细胞中同源框蛋白 PROX1 的表达具有负调控作用。

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069030081

Munki Jeong, Euitaek Jung, Sukjin Oh, Soon Young Shin

{"title":"Homeobox Protein PROX1 Expression is Negatively Regulated by Histone Deacetylase 1 and c-JUN Complex in MDA-MB-231 Human Breast Cancer Cells.","authors":"Munki Jeong, Euitaek Jung, Sukjin Oh, Soon Young Shin","doi":"10.14712/fb2023069030081","DOIUrl":"10.14712/fb2023069030081","url":null,"abstract":"Prospero homeobox 1 (PROX1) is a member of the homeobox transcription factor family that plays a critical role in the development of multiple tissues and specification of cell fate. PROX1 expression is differentially regulated based on the cellular context and plays an antagonistic role as a tumour promoter or suppressor in different tumour types. In human breast cancer, PROX1 expression is suppress-ed; however, the molecular mechanism by which it is down-regulated remains poorly understood. Here, we show that ectopic expression of PROX1 reduces the motility and invasiveness of MDA-MB-231 human breast cancer cells, suggesting that PROX1 functions as a negative regulator of tumour invasion in MDA-MB-231 cells. Treatment with histone deacetylase (HDAC) inhibitors up-regulates PROX1 mRNA and protein expression levels. Knockdown of HDAC1 using short hairpin RNA also up-regulates PROX1 mRNA and protein expression levels. We found that HDAC1 interacted with c-JUN at the activator protein (AP)-1-binding site located at -734 to -710 in the PROX1 promoter region to suppress PROX1 expression. In addition, c-JUN N-terminal kinase-mediated c-JUN phosphorylation was found to be crucial for silencing PROX1 expression. In conclusion, PROX1 expression can be silenced by the epigenetic mechanism involved in the complex formation of HDAC1 and c-JUN at the AP-1 site in the PROX1 promoter region in MDA-MB-231 human breast cancer cells. Therefore, this study revealed the epigenetic regulatory mechanism involved in the suppression of PROX1 expression in breast cancer cells.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 3","pages":"81-90"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139424547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Radiation-Induced Lymphopoenia and Treatment Outcome in Hereditary Breast Cancer Patients. 遗传性乳腺癌患者的放射诱导淋巴细胞减少症与治疗结果

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069030091

Soňa Argalácsová, Ľudmila Křížová, Martin Matějů, Dominika Svobodová, Michal Vočka

{"title":"Radiation-Induced Lymphopoenia and Treatment Outcome in Hereditary Breast Cancer Patients.","authors":"Soňa Argalácsová, Ľudmila Křížová, Martin Matějů, Dominika Svobodová, Michal Vočka","doi":"10.14712/fb2023069030091","DOIUrl":"10.14712/fb2023069030091","url":null,"abstract":"Many breast cancer (BC) predisposition genes encode proteins involved in DNA damage repair (DDR). Identification of germline pathogenic va-riants (PV) in DDR genes raises the question whether their presence can influence the treatment outcomes and potential radiation-induced toxicity in their carriers treated by adjuvant radiotherapy, which has not yet been answered conclusively. We retrospectively examined records of 213 BC patients treated by adjuvant radiotherapy, including 39 (18.3 %) BRCA1/2 PV carriers, 25 carriers (11.7 %) of PV in other breast cancer-predisposing genes, and 149 (70 %) non-carriers. Our goal was to examine 5-year disease-free survival (5y DFS) rates among the study groups and determine the impact of radiotherapy-induced lymphopoenia (RIL) on this outcome. While we found no significant difference in 5y DFS between non-carriers and carriers of BRCA mutations (86.4 % vs 78.4 % P = 0.24) or between non-carriers and other studied mutations (86.4 % vs 93.3 %; P = 0.27), respectively, we observed that the entire group of PV carriers had a significantly lower proportion of patients without RIL (P = 0.04) than the non-carriers. In contrast, subsequent analyses indicated a non-significant trend toward an increased 5y DFS in PV carriers with RIL. Our single-centre study indicated that the presence of PV in BC patients has an insignificant impact on DFS but can reduce the risk of RIL associated with adjuvant radiotherapy. It remains unclear whether this may result from the paradoxical activation of anti-tumour immunity in PV carriers with higher lymphocyte consumption resulting from higher immune effectiveness.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 3","pages":"91-98"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139424548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Germline Pathogenic Variants in Squamous Cell Carcinoma of the Head and Neck. 头颈部鳞状细胞癌的基因致病变异

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069040107

Tereza Drbohlavová, Soňa Argalácsová, Jana Soukupová, Michal Vočka

{"title":"Germline Pathogenic Variants in Squamous Cell Carcinoma of the Head and Neck.","authors":"Tereza Drbohlavová, Soňa Argalácsová, Jana Soukupová, Michal Vočka","doi":"10.14712/fb2023069040107","DOIUrl":"10.14712/fb2023069040107","url":null,"abstract":"Head and neck squamous cell carcinoma (HNSCC) presents a significant global health problem with variable geographic distribution and risk factors, including tobacco and alcohol abuse, human papillomavirus infections, and genetic predisposition. While the majority of cases are sporadic, several well-defined hereditary syndromes have been associated with a higher risk of developing HNSCC including Li-Fraumeni syndrome, Fanconi anaemia, Bloom syndrome, familial atypical multiple mole melanoma, and dyskeratosis congenita. There is also evidence of familial clusters of HNSCC, suggesting a genetic component in the development of the disease. Germ-line genetic testing in HNSCC using next-generation sequencing has revealed a wide range of germline variants, some of which were not anticipated based on standard guidelines. These variants may influence treatment decisions and have the potential to be targeted with precision medicine in the future. Despite these advances, routine germline genetic testing for HNSCC is not currently recommended and remains reserved for HNSCC cases with early onset or strong family cancer history. However, the increasing availability of germline genetic testing warrants development of more comprehensive and standardized testing protocols. Germline genetic testing also has the potential to influence precision-guided treatment in HNSCC patients carrying germline pathogenic variants.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 4","pages":"107-115"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139971524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Pilot Study on the Uptake of Propidium Iodide and YO-PRO-1 Iodide through the Pannexin Channels in Wallachian Frozen-Thawed Ram Spermatozoa. 关于瓦拉几亚冷冻解冻公羊精子通过 Pannexin 通道摄取碘化丙啶和 YO-PRO-1 碘化物的试验性研究

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069040127

Filipp Georgijevič Savvulidi, Martin Ptáček, Anežka Málková, Elena Golovina, Jan Pytlík, Martina Janošíková, Szabolcs Nagy, Joăo Pedro Barbas, Luděk Stádník, Karina Savvulidi Vargová

{"title":"A Pilot Study on the Uptake of Propidium Iodide and YO-PRO-1 Iodide through the Pannexin Channels in Wallachian Frozen-Thawed Ram Spermatozoa.","authors":"Filipp Georgijevič Savvulidi, Martin Ptáček, Anežka Málková, Elena Golovina, Jan Pytlík, Martina Janošíková, Szabolcs Nagy, Joăo Pedro Barbas, Luděk Stádník, Karina Savvulidi Vargová","doi":"10.14712/fb2023069040127","DOIUrl":"10.14712/fb2023069040127","url":null,"abstract":"Propidium iodide (PI) and YO-PRO-1 (YPI) dyes are routinely used to determine sperm viability in many livestock species. It is commonly accepted that these dyes penetrate only sperm cells with damaged plasma membranes. Recently, however, the mechanism of dye uptake unrelated to damaged plasma membranes, but instead related to pannexin channels in dog and stallion sperm cells was demonstrated. This pilot study aimed to evaluate the role of pannexins in the uptake of PI and YPI dyes on Wallachian frozen-thawed ram spermatozoa by flow cytometry using probenecid, a specific inhibitor of pannexin channels. Additionally, the expression of pannexins in Wallachian sperm was evaluated directly (by qRT-PCR). The results demonstrate the active role of pannexin channels in the uptake of PI and YPI dyes on frozen-thawed Wallachian ram sperm. In conclusion, when using the PI or YPI exclusion assay to determine Wallachian frozen-thawed ram sperm viability, the danger of overestimating the number of spermatozoa with the damaged plasma membrane must be considered. The observed breed-specific, and more importantly, individual differences in gene expression as well as in dye uptake indicate the need for further studies.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 4","pages":"127-132"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139971522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Delivery-Associated Changes in the Levels of Inflammatory Molecules in Newborns. 新生儿炎症分子水平与分娩相关的变化

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069010001

Marek Mojžíšek, Michaela Šibíková, Martin Pánek, Petr Janec, Martin Haluzík, Jan Živný, Jan Janota

{"title":"Delivery-Associated Changes in the Levels of Inflammatory Molecules in Newborns.","authors":"Marek Mojžíšek, Michaela Šibíková, Martin Pánek, Petr Janec, Martin Haluzík, Jan Živný, Jan Janota","doi":"10.14712/fb2023069010001","DOIUrl":"10.14712/fb2023069010001","url":null,"abstract":"Inflammation is considered a fundamental process accompanying physiological human birth, also playing a role in perinatal pathologies. The goal of the study was to assess the concentrations of inflammatory molecules with respect to the mode of delivery and dynamics of inflammatory molecules in neonatal samples in the first 48-72 hours of life. The concentrations of inflammatory cytokines were measured using the Luminex®xMAP multi-analyte profiling platform in cord blood and peripheral neonatal blood. Study groups included newborns delivered spontaneously (spontaneous group) and via elective caesarean section (elective group). Cord blood concentrations of interleukin 6 (IL-6) and procalcitonin were significantly higher (P < 0.0001) in the spontaneous group compared to the elective group. Neonatal blood concentrations of tumour necrosis factor (TNF) from the elective group were significantly higher com-pared to the spontaneous group (P = 0.0077). The concentrations of procalcitonin and TNF significantly increased within the first 48 to 72 hours following either mode of delivery. IL-6 and IL-18 were significantly higher in neonatal compared to umbilical cord blood in the elective group only, while the increase in the spontaneous group did not reach statistical significance. The concentrations of IL-1α, IL-1β, IL-17A and IL-22 did not show significant differen-ces between the spontaneous and elective groups as well as between umbilical cord and neonatal blood. Our findings show physiological differences in the levels of inflammatory molecules following spontaneous vaginal delivery and elective caesarean section. The results can be used as baseline values for the research of various pathologies in newborns.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 1","pages":"1-5"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92153417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

De novo Transcriptome Analysis and Gene Expression Profiling of Corylus Species. 新的 Corylus 树种转录组分析和基因表达谱分析。

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069030099

Salih Ulu, Zehra Omeroglu Ulu, Aysun Akar, Nehir Ozdemir Ozgenturk

{"title":"De novo Transcriptome Analysis and Gene Expression Profiling of Corylus Species.","authors":"Salih Ulu, Zehra Omeroglu Ulu, Aysun Akar, Nehir Ozdemir Ozgenturk","doi":"10.14712/fb2023069030099","DOIUrl":"10.14712/fb2023069030099","url":null,"abstract":"Hazelnut (Corylus), which has high commercial and nutritional benefits, is an important tree for producing nuts and nut oil consumed as ingredient especially in chocolate. While Corylus avellana L. (Euro-pean hazelnut, Betulaceae) and Corylus colurna L. (Turkish hazelnut, Betulaceae) are the two common hazelnut species in Europe, C. avellana L. (Tombul hazelnut) is grown as the most widespread hazelnut species in Turkey, and C. colurna L., which is the most important genetic resource for hazelnut breeding, exists naturally in Anatolia. We generated the transcriptome data of these two Corylus species and used these data for gene discovery and gene expression profiling. Total RNA from young leaves, flowers (male and female), buds, and husk shoots of C. avellana and C. colurna were used for two different libraries and were sequenced using Illumina HiSeq4000 with 100 bp paired-end reads. The transcriptome data 10.48 and 10.30 Gb of C. avellana and C. colurna, respectively, were assembled into 70,265 and 88,343 unigenes, respectively. These unigenes were functionally annotated using the TRAPID platform. We identified 25,312 and 27,051 simple sequen-ce repeats (SSRs) for C. avellana and C. colurna, respectively. TL1, GMPM1, N, 2MMP, At1g29670, CHIB1 unigenes were selected for validation with qPCR. The first de novo transcriptome data of C. co-lurna were used to compare data of C. avellana of commercial importance. These data constitute a valuable extension of the publicly available transcriptomic resource aimed at breeding, medicinal, and industrial research studies.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 3","pages":"99-106"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139424545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0