Folia Biologica最新文献_第4页

Erratum - Tri-Lineage Differentiation of NTERA2 Clone D1 Cells towards Neural, Hepatic and Osteogenic Lineages in Vitro. NTERA2克隆D1细胞在体外向神经、肝和成骨谱系的三谱系分化。

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069010040

Balázs Szeky, B Mayer, M Gyongy, A Hajdara, S Barsi, S Karpati, K Nemeth

引用次数: 0

Technical Note Cell Dysplasia - Cell Dysplastic Features (A Morphological Note). 技术注释细胞发育不良-细胞发育不良特征(形态学注释)。

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069010034

Karel Smetana, Dana Mikulenková, Hana Klamová, Josef Karban, Marek Trněný

引用次数: 0

Redox Status of Erythrocytes as an Important Factor in Eryptosis and Erythronecroptosis. 红细胞的氧化还原状态是红细胞色素沉着和红细胞色素沉着的重要因素

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069040116

Anton Tkachenko, Ondřej Havránek

{"title":"Redox Status of Erythrocytes as an Important Factor in Eryptosis and Erythronecroptosis.","authors":"Anton Tkachenko, Ondřej Havránek","doi":"10.14712/fb2023069040116","DOIUrl":"10.14712/fb2023069040116","url":null,"abstract":"Overall, reactive oxygen species (ROS) signalling significantly contributes to initiation and mo-dulation of multiple regulated cell death (RCD) pathways. Lately, more information has become available about RCD modalities of erythrocytes, including the role of ROS. ROS accumulation has therefore been increasingly recognized as a critical factor involved in eryptosis (apoptosis of erythrocytes) and erythro-necroptosis (necroptosis of erythrocytes). Eryptosis is a Ca2+-dependent apoptosis-like RCD of erythrocytes that occurs in response to oxidative stress, hyperosmolarity, ATP depletion, and a wide range of xenobiotics. Moreover, eryptosis seems to be involved in the pathogenesis of multiple human diseases and pathological processes. Several studies have reported that erythrocytes can also undergo necroptosis, a lytic RIPK1/RIPK3/MLKL-mediated RCD. As an example, erythronecroptosis can occur in response to CD59-specific pore-forming toxins. We have systematically summarized available studies regarding the involvement of ROS and oxidative stress in these two distinct RCDs of erythrocytes. We have focused specifically on cellular signalling pathways involved in ROS-mediated cell death decisions in erythrocytes. Furthermore, we have summarized dysregulation of related erythrocytic antioxidant defence systems. The general concept of the ROS role in eryptotic and necroptotic cell death pathways in erythrocytes seems to be established. However, further studies are required to uncover the complex role of ROS in the crosstalk and interplay between the survival and RCDs of erythrocytes.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 4","pages":"116-126"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139971525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathogenesis of Collagen-Induced Arthritis: Role of Immune Cells with Associated Cytokines and Antibodies, Comparison with Rheumatoid Arthritis. 胶原诱发关节炎的发病机制：免疫细胞与相关细胞因子和抗体的作用，与类风湿关节炎的比较。

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069020041

Monika Šteigerová, Martin Šíma, Ondřej Slanař

{"title":"Pathogenesis of Collagen-Induced Arthritis: Role of Immune Cells with Associated Cytokines and Antibodies, Comparison with Rheumatoid Arthritis.","authors":"Monika Šteigerová, Martin Šíma, Ondřej Slanař","doi":"10.14712/fb2023069020041","DOIUrl":"https://doi.org/10.14712/fb2023069020041","url":null,"abstract":"Collagen-induced arthritis is the most com-mon in vivo model of rheumatoid arthritis used for investigation of new potential therapies in preclinical research. Rheumatoid arthritis is a systemic inflammatory and autoimmune disease affecting joints, accompanied by significant extra-articular symptoms. The pathogenesis of rheumatoid arthritis and collagen-induced arthritis involves a so far properly unexplored network of immune cells, cytokines, antibodies and other factors. These agents trigger the autoimmune response leading to polyarthritis with cell infiltration, bone and cartilage degeneration and synovial cell proliferation. Our review covers the knowledge about cytokines present in the rat collagen-induced arthritis model and the factors affecting them. In addition, we provide a comparison with rheumatoid arthritis and a description of their important effects on the development of both diseases. We discuss the crucial roles of various immune cells (subtypes of T and B lymphocytes, dendritic cells, monocytes, macrophages), fibroblast-like synoviocy-tes, and their related cytokines (TNF-α, IL-1β, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12, IL-17, IL-23, GM-CSF, TGF-β). Finally, we also focus on key antibodies (rheu-matoid factor, anti-citrullinated protein antibodies, anti-collagen II antibodies) and tissue-degrading enzymes (matrix metalloproteinases).","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 2","pages":"41-49"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138795230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Dihydropyrimidinase-like 3 Gene Expression by MicroRNAs in PC12 Cells with Induced Ischaemia and Hypothermia. 微RNA对缺血和低温诱导下PC12细胞中二氢嘧啶酶样3基因表达的调控

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069020069

Kisang Kwon, Ji-Hye Song, Hyewon Park, O-Yu Kwon, Seung-Whan Kim

引用次数: 0

Astaxanthin Induces Apoptosis in Human Osteosarcoma MG-63 Cells. 虾青素诱导人骨肉瘤 MG-63 细胞凋亡

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069050186

Guangyu Wang, Xu Tian, Lintao Liu, Jingming Dong

{"title":"Astaxanthin Induces Apoptosis in Human Osteosarcoma MG-63 Cells.","authors":"Guangyu Wang, Xu Tian, Lintao Liu, Jingming Dong","doi":"10.14712/fb2023069050186","DOIUrl":"https://doi.org/10.14712/fb2023069050186","url":null,"abstract":"We explored the mechanism of human osteosarcoma MG-63 cell apoptosis induced by asta-xanthin. The MTT assay was used to detect the effect of astaxanthin on cell viability. Morphological changes associated with apoptosis were observed after DAPI staining. Early and late stages of apoptosis were detected by flow cytometry with annexin V-FITC/PI staining. Activation of caspases-8, -9 and -3 was detected by enzyme activity in vitro. Changes in the mitochondrial membrane potential were detected by MitoCapture staining. Western blot was used to detect the cleavage of PARP, which is a caspase-3 substrate, the release of cytochrome c and Smac into the cytosol, the translocation of pro-apoptotic proteins Bax and Bak, and the expression of mitochondrial pathway-related proteins. The translocation of Bax was also detected by immunofluorescence assay. Astaxanthin significantly inhibited the viability of human osteosarcoma MG-63 cells with an IC50 value of 12.36 μg/ml. The DAPI-stained cells showed characteristic apoptotic morphological changes - cell shrinkage, cell membrane blebbing, nuclear condensation, and apoptotic body formation. Cytochrome c and Smac were released from mitochondria to the cytosol. Pro-apoptotic proteins Bax and Bak were rapidly translocated to mitochondria after six hours of astaxanthin action. Caspases-9 and -3 were activated and PARP was cleaved. The expression of anti-apoptotic proteins Bcl-2, Bcl-xL and XIAP was significantly decreased. Astaxanthin induced human osteosarcoma MG-63 cell apoptosis through the mitochondria-mediated endogenous apoptosis pathway.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 5-6","pages":"186-193"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140853049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autotaxin and Lysophosphatidic Acid Signalling: the Pleiotropic Regulatory Network in Cancer. Autotaxin 和溶血磷脂酸信号：癌症中的多向调控网络

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069050149

Ondřej Vít, Jiří Petrák

引用次数: 0

Many Ways to the Cell Cycle Exit after Inhibition of CDK4/6. CDK4/6 抑制后细胞周期退出的多种途径

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069050194

Libor Macůrek

引用次数: 0

Study of the Methylation of Bovine GSTP1 Gene under the Influence of Pesticide Mospilan 20SP Alone and in Combination with Pesticide Orius 25EW. 农药Mospilan 20SP单独及与农药Orius 25EW联合作用下牛GSTP1基因甲基化的研究

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069010006

Jana Halušková, Beáta Holečková, Lenka Luptáková, Ján Košuth, Viera Schwarzbacherová, Martina Galdíková, Simona Koleničová

{"title":"Study of the Methylation of Bovine GSTP1 Gene under the Influence of Pesticide Mospilan 20SP Alone and in Combination with Pesticide Orius 25EW.","authors":"Jana Halušková, Beáta Holečková, Lenka Luptáková, Ján Košuth, Viera Schwarzbacherová, Martina Galdíková, Simona Koleničová","doi":"10.14712/fb2023069010006","DOIUrl":"10.14712/fb2023069010006","url":null,"abstract":"DNA methylation, one of the most studied epigenetic mechanisms, when present in the promoter region of genes, causes inhibition of gene expression, and conversely, hypomethylation of these regions enables gene expression. DNA methylation is susceptible to nutritional and environmental influences, and undesirable alterations in methylation patterns manifested in changes in the expression of relevant genes can lead to pathological consequences. In the present work, we studied the methylation status of the bovine GSTP1 gene under the influence of pesticide Mospilan 20SP alone and in combination with pesticide Orius 25EW in in vitro proliferating bovine lymphocytes. We employed methylation-specific PCR, and when studying the effect of pesticide combinations, we also used its real-time version followed by a melting procedure. Our results showed that Mospilan 20SP alone at 5, 25, 50, and 100 µg.ml-1 and 5, 10, 25, and 50 µg.ml-1 for the last 4 and 24 hours of culture with in vitro proliferating bovine lymphocytes, respectively, did not induce methylation of the bovine GSTP1 gene. The same results were revealed when studying the effect of the combination of the pesticides added to the lymphocyte cultures for the last 24 hours of cultivation in the following amounts: 1.25, 2.5, 5, 10, and 25 µg.ml-1 of Mospilan 20SP and 1.5, 3, 6, 15, and 30 µg.ml-1 of Orius 25EW. We have also revealed that the less laborious real-time MSP followed by a melting procedure may replace MSP for studying the methylation status of the GSTP1 gene.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 1","pages":"6-12"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92153420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Homeobox Protein PROX1 Expression is Negatively Regulated by Histone Deacetylase 1 and c-JUN Complex in MDA-MB-231 Human Breast Cancer Cells. 组蛋白去乙酰化酶 1 和 c-JUN 复合物对 MDA-MB-231 人乳腺癌细胞中同源框蛋白 PROX1 的表达具有负调控作用。

IF 0.6 4区医学

Folia Biologica Pub Date : 2023-01-01 DOI: 10.14712/fb2023069030081

Munki Jeong, Euitaek Jung, Sukjin Oh, Soon Young Shin

{"title":"Homeobox Protein PROX1 Expression is Negatively Regulated by Histone Deacetylase 1 and c-JUN Complex in MDA-MB-231 Human Breast Cancer Cells.","authors":"Munki Jeong, Euitaek Jung, Sukjin Oh, Soon Young Shin","doi":"10.14712/fb2023069030081","DOIUrl":"10.14712/fb2023069030081","url":null,"abstract":"Prospero homeobox 1 (PROX1) is a member of the homeobox transcription factor family that plays a critical role in the development of multiple tissues and specification of cell fate. PROX1 expression is differentially regulated based on the cellular context and plays an antagonistic role as a tumour promoter or suppressor in different tumour types. In human breast cancer, PROX1 expression is suppress-ed; however, the molecular mechanism by which it is down-regulated remains poorly understood. Here, we show that ectopic expression of PROX1 reduces the motility and invasiveness of MDA-MB-231 human breast cancer cells, suggesting that PROX1 functions as a negative regulator of tumour invasion in MDA-MB-231 cells. Treatment with histone deacetylase (HDAC) inhibitors up-regulates PROX1 mRNA and protein expression levels. Knockdown of HDAC1 using short hairpin RNA also up-regulates PROX1 mRNA and protein expression levels. We found that HDAC1 interacted with c-JUN at the activator protein (AP)-1-binding site located at -734 to -710 in the PROX1 promoter region to suppress PROX1 expression. In addition, c-JUN N-terminal kinase-mediated c-JUN phosphorylation was found to be crucial for silencing PROX1 expression. In conclusion, PROX1 expression can be silenced by the epigenetic mechanism involved in the complex formation of HDAC1 and c-JUN at the AP-1 site in the PROX1 promoter region in MDA-MB-231 human breast cancer cells. Therefore, this study revealed the epigenetic regulatory mechanism involved in the suppression of PROX1 expression in breast cancer cells.","PeriodicalId":12281,"journal":{"name":"Folia Biologica","volume":"69 3","pages":"81-90"},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139424547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0