Evidence-based Complementary and Alternative Medicine最新文献_第5页

In Vitro Cytotoxicity of Reproductive Stage Withania somnifera Leaf and Stem on HepG2 Cell Line 生殖期的薇甘菊叶和茎对 HepG2 细胞株的体外细胞毒性

4区医学

Evidence-based Complementary and Alternative Medicine Pub Date : 2023-12-26 DOI: 10.1155/2023/8832166

Lali Lingfa, Aravinda Tirumala, Srinivas Ankanagari

{"title":"In Vitro Cytotoxicity of Reproductive Stage Withania somnifera Leaf and Stem on HepG2 Cell Line","authors":"Lali Lingfa, Aravinda Tirumala, Srinivas Ankanagari","doi":"10.1155/2023/8832166","DOIUrl":"https://doi.org/10.1155/2023/8832166","url":null,"abstract":"Background. The ayurvedic plant Withania somnifera, a member of the Solanaceae family, has been used as a remedy for diverse health problems, including cancer. Objectives. The objective of this investigation was to conduct a comparative analysis of the in vitro cytotoxic properties of methanolic extracts derived from the leaf, stem, and root of W. somnifera on HepG2 and L929 cell lines. Methods. Methanolic extracts were obtained using the Soxhlet extraction method. To assess the in vitro anticancer action on the HepG2 and L929 cell lines, an MTT assay was performed. Changes in cell morphology were observed using an inverted microscope. Results. The MTT assay results indicated that the leaf, stem, and root methanolic extracts of W. somnifera showed significantly higher in vitro cytotoxicity in HepG2 cells, with IC50 values of 43.06 ± 0.615, 45.60 ± 0.3, and 314.4 ± 0.795 μg/mL than in L929 cell lines with 78.77 ± 0.795, 90.55 ± 0.800, and 361.70 ± 0.795 μg/mL, respectively. The leaf methanolic extract was the most effective, followed by the stem methanolic extract in the HepG2 cell line. Conclusion. The results of our study have confirmed that the methanolic extracts of both the leaf and stem of W. somnifera exhibit significant in vitro cytotoxicity in HepG2 cell lines, while displaying no significant cytotoxicity in the L929 cell line. Furthermore, the data obtained from the MTT assay indicate that the leaf methanolic extract possesses a more potent cytotoxic activity than the stem methanolic extract with respect to the HepG2 cell line. Further studies on the identification and isolation of bioactive metabolites are required to explore the mechanisms underlying their in vitro cytotoxicity.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139055968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimalarial Properties of Sida cordifolia L. Leaf Extract in Mice: Survivability Depends Less on Parasitaemia Suppression Sida cordifolia L. 叶提取物在小鼠体内的抗疟特性：存活率较少依赖于寄生虫血症抑制作用

4区医学

Evidence-based Complementary and Alternative Medicine Pub Date : 2023-12-23 DOI: 10.1155/2023/5560711

Samuel Ansong, Desmond Nkrumah, Reinhard Isaac Nketia, Samuel Oppong Bekoe, Abena Amponsaa Brobbey, Evelyn Asante-Kwatia, Gustav Komlaga

{"title":"Antimalarial Properties of Sida cordifolia L. Leaf Extract in Mice: Survivability Depends Less on Parasitaemia Suppression","authors":"Samuel Ansong, Desmond Nkrumah, Reinhard Isaac Nketia, Samuel Oppong Bekoe, Abena Amponsaa Brobbey, Evelyn Asante-Kwatia, Gustav Komlaga","doi":"10.1155/2023/5560711","DOIUrl":"https://doi.org/10.1155/2023/5560711","url":null,"abstract":"Sida cordifolia has been used to treat malaria in Ghana albeit without scientific evidence of antimalarial activity and safety. This work aimed to assess the antimalarial properties and acute toxicity of the aqueous leaf extract of S. cordifolia in murine models. Aqueous extract of the plant was analysed for both suppressive and curative antimalarial properties in chloroquine-sensitive ANKA strains of rodent Plasmodium berghei-infected mice. Acute toxicity evaluation was performed in rats according to the OECD 425 guidelines. The extract displayed antiplasmodial activity in vivo with ED50 of 117.49 ± 15.22 mg/kg and 144.84 ± 18.17 mg/kg in suppressive and curative studies, respectively. The highest % parasitaemia suppression exerted was 76.90 ± 0.64% and 61.50 ± 0.97%, respectively, in the suppressive and curative studies. Survival of infected mice treated with the extract was significantly prolonged. This was dependent on the dose of the extract but imperfectly related to the % parasitaemia suppression. Related antimalarial parameters including percentage hematocrit, changes in body weight, and temperature of experimental mice indicated alleviation of malarial symptoms of treated animals. The extract did not show toxicity in rats. Sida cordifolia L. has antimalarial properties, and was safe. It suppressed parasitaemia in both suppressive and curative studies, was not toxic to animals and prolonged the life of infected animals under treatment. This, therefore, justifies the traditional use of S. cordifolia for the treatment of malaria in Ghana.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"80 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139022460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cardioprotective Effects of Solidago microglossa DC. in Nicotine-Treated Hypertensive Rats 尼古丁治疗的高血压大鼠体内实心草（Solidago microglossa DC.）的心脏保护作用

4区医学

Evidence-based Complementary and Alternative Medicine Pub Date : 2023-12-22 DOI: 10.1155/2023/6611569

Katiana Simões Lopes, A. A. M. Marques, Karyne Garcia Tafarelo Moreno, A. C. dos Santos, R. I. Souza, E. Lourenço, Rodrigo Juliano Oliveira, R. Gomes, F. Lívero, A. Gasparotto Júnior

{"title":"Cardioprotective Effects of Solidago microglossa DC. in Nicotine-Treated Hypertensive Rats","authors":"Katiana Simões Lopes, A. A. M. Marques, Karyne Garcia Tafarelo Moreno, A. C. dos Santos, R. I. Souza, E. Lourenço, Rodrigo Juliano Oliveira, R. Gomes, F. Lívero, A. Gasparotto Júnior","doi":"10.1155/2023/6611569","DOIUrl":"https://doi.org/10.1155/2023/6611569","url":null,"abstract":"Solidago microglossa DC. (Asteraceae), “arnica brasileira,” is a Brazilian species popularly used to treat hypertension or renal ailments. This study investigated the cardioprotective effects of standardized S. microglossa extract (EESM) in nicotine-treated spontaneously hypertensive rats (SHRs). Moreover, the molecular mechanisms involved in the cardiovascular effects were also investigated. The acute toxicity was evaluated in female Wistar rats. Afterwards, six-month-old male spontaneously hypertensive rats received the EESM (14, 28, and 56 mg/kg), hydrochlorothiazide (25 mg/kg), and vehicle (filtered water; 0.1 mL/100 g) once daily for 28 days. All treatments were associated with 1.8 mg/kg of nicotine. At the end of the experimental period, the renal function, electrocardiographic profile, blood pressure, ventricular function, biochemical parameter, and mesenteric vascular bed reactivity were evaluated. Relative organ weights and cardiac morphometry were also investigated. Nicotine treatment in 6-month-old SHRs induced a significant reduction in renal function, with reduced urinary volume and lower renal elimination of sodium and creatinine. In addition, serum markers of the redox state and blood pressure levels remained significantly elevated, contributing to changes in vascular reactivity and left ventricular hypertrophy associated with reduced ventricular function. After 28 days of treatment, we found that the highest dose of EESM could mitigate all renal and cardiovascular changes developed by the nicotine-treated hypertensive rats. This study presented EESM as a possible cardioprotective drug that prevents cardiovascular dysfunctions in nicotine-treated hypertensive rats. Our data suggest EESM as a potential adjuvant therapy when cardioprotective effects are required.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":"59 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138945767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retracted: Clinical Study of Stellate Ganglion Block Combined with General Anesthesia on Hemodynamics, Cognitive Function, and Gastrointestinal Function in Elderly Patients Undergoing Partial Hepatectomy. 撤回：星状神经节阻滞联合全身麻醉对接受部分肝切除术的老年患者血液动力学、认知功能和胃肠功能影响的临床研究

4区医学

Evidence-based Complementary and Alternative Medicine Pub Date : 2023-12-13 eCollection Date: 2023-01-01 DOI: 10.1155/2023/9826513