Evidence-based Complementary and Alternative Medicine最新文献

{"title":"Retracted: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway.","authors":"Evidence-Based Complementary And Alternative Medicine","doi":"10.1155/2024/9831531","DOIUrl":"10.1155/2024/9831531","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2020/6538156.].</p>","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11293942/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141874562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of In Vivo Antidiarrheal Activities of 80% Methanol Extract and Solvent Fractions of Peels of <i>Colocasia esculenta</i> (Araceae).","authors":"Temesgen Obsa Terfa, Fekadu Abera Kebede, Monas Kitessa Beyene, Tilahun Tesfaye Abebe, Abebe Basazn Mekuria, Jibril Seid Yesuf","doi":"10.1155/2024/2728282","DOIUrl":"10.1155/2024/2728282","url":null,"abstract":"<p><strong>Background: </strong>Diarrhea is the common gastrointestinal disorder accounting for 2.5 billion episodes and 1.5 million deaths annually. Limitations and inaccessibility of currently available medications are the main problem associated with treatment of diarrhea. Hence, medicinal plants are usually preferred to manage diarrhea because they may contain constituents with high activity and fewer side effects. Even though the dose, safety, and efficacy of <i>Colocasia esculenta</i> (L.) Schott are not substantiated scientifically, several societies use it for the treatment of diarrhea.</p><p><strong>Objective: </strong>This study was targeted at exploring the in vivo antidiarrheal activities of 80% methanol extract and solvent fractions of peels of <i>Colocasia esculenta</i> (L.) Schott in Swiss albino mice.</p><p><strong>Methods: </strong>The plant was collected and extracted with 80% methanol, followed by fractionation with distilled water, chloroform, and diethyl ether. Castor oil-induced diarrhea, enteropooling, and motility tests were used to evaluate antidiarrheal activity. The test groups received graded doses of 100 mg/kg, 200 mg/kg, and 400 mg/kg. Negative controls received 10 ml/kg of 2% Tween 80 while positive controls received loperamide (3 mg/kg) or atropine (5 mg/kg).</p><p><strong>Results: </strong>The crude and solvent fractions of the plant extract have induced significant effects in reduction of the number and weight of wet stools at all tested doses. However, delay in onset of diarrhea was observed only at 400 mg/kg (<i>P</i> < 0.001) for both crude extract and solvent fractions. In antienteropooling test, 80% methanol extract and solvent fractions have significantly reduced the weight and volume of intestinal contents, especially at 200 mg/kg and 400 mg/kg. Regarding the antimotility test, the crude extract reduced motility at all tested doses, whereas the solvent fractions reduced intestinal motility mainly at 400 mg/kg (<i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>The study has revealed that the 80% methanol extract and solvent fractions of the plant possess antidiarrheal activities supporting the traditional antidiarrheal claims of the plant.</p>","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11259501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141733906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-<i>Helicobacter pylori</i> Effects of <i>Thymus caramanicus</i> Jalas Essential Oils: A New Antimicrobial Approach.","authors":"Sepehr Asadi, Ebrahim Rahimi, Amir Shakerian","doi":"10.1155/2024/3627074","DOIUrl":"10.1155/2024/3627074","url":null,"abstract":"<p><strong>Background: </strong><i>Helicobacter pylori</i> are the principal causative factor in the etiological factors of chronic, active, or type B gastritis; peptic and duodenal ulcers; stomach carcinoma; and epithelial tissue lymphoid malignancies. It infects more than half of the population worldwide. To reduce <i>H. pylori</i> production, pharmacological therapy of <i>H. pylori</i> diseases typically involves using threefold treatment methods. However, as a result of such therapy, antimicrobial resistance is commonly developed. Alternative therapeutics for <i>H. pylori</i> diseases are thus of particular interest.</p><p><strong>Methods: </strong>Thyme essential oils (EOs) obtained from <i>T. caramanicus</i> Jalas plants in Iran were tested for antibacterial activity against <i>H. pylori</i> obtained from 320 poultry specimens in this investigation. Antibacterial activity was measured using inhibition zones, minimum inhibitory concentrations (MICs), and minimum bactericidal concentrations (MBCs). The impact of <i>T. caramanicus</i> Jalas essential oils on <i>H. pylori</i> isolate <i>cagA</i>, <i>vacA</i>, and <i>babA2</i> gene expression was evaluated using a quantitative real-time PCR method (<i>p</i> < 0.05).</p><p><strong>Results: </strong>The chemical content of these EOs varied significantly according to chromatographic examination. Thymol, carvacrol, and terpinene-4-ol are the most abundant components in these EOs. <i>H. pylori</i> was recognized as a <i>Helicobacter</i> species with a 175-bp PCR product of 16S rRNA in 20/20 (100%). According to PCR results, all 20 (100%) isolates belonged to <i>H. pylori</i>. The EOs inhibited <i>H. pylori</i> in a dose-dependent manner, with <i>T. caramanicus</i> Jalas being the most effective, followed by pterygium EOs in decreasing order. At 8 mg/mL of <i>T. caramanicus</i> Jalas EOs, IZs against <i>H. pylori</i> were 27.4 ± 0.42 mm, and at 8 mg/mL of pterygium, IZs against <i>H. pylori</i> were 1 ± 0.02. <i>T. caramanicus</i> Jalas essential oils were used to treat all bacteria, and the findings showed that <i>T. caramanicus</i> Jalas had a substantial inhibitory impact on the expression of <i>cagA</i>, <i>vacA</i>, and <i>babA2</i> virulence-related genes (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>In a dose-dependent manner, the EOs of <i>T. caramanicus</i> Jalas EO demonstrated a high degree of antimicrobial property against <i>H. pylori</i> bacteria. The most efficient EOs were those from <i>T. caramanicus</i> Jalas with relative concentrations of thymol and carvacrol, followed by the coumarin-dominated pterygium EO with reduced antibacterial activity.</p>","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11182686/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141418501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and Antioxidant Compounds from the Root Extract of <i>Aloe debrana</i>.","authors":"Tokuma Getahun, Joydeep Das, Parames C Sil, Neeraj Gupta","doi":"10.1155/2024/6651648","DOIUrl":"10.1155/2024/6651648","url":null,"abstract":"<p><p>This study was conducted to isolate and identify the chemical compounds from the roots of <i>Aloe debrana</i> (L.) and evaluate their antioxidant and antibacterial activities. From the acetone (99.5%) extract of the roots of this plant, four anthraquinones, such as chrysophanol (<b>1</b>), asphodeline (<b>2</b>), aloesaponarin I (<b>5</b>), and laccaic acid D-methyl ester (<b>6</b>), and a new catechol derivative, 5-allyl-3-methoxybenzene-1,2-diol (<b>3</b>), were isolated and elucidated by different chromatographic and spectroscopic methods together with linoleic acid (<b>4</b>), respectively. Compounds <b>2</b>, <b>3,</b> and <b>4</b> were reported here for the first time from this plant and compound <b>3</b> from the genus <i>Aloe</i>. The compounds were evaluated for their antioxidant activity using H₂O₂ and DPPH assays and bactericidal activity against <i>S. aureus</i> and <i>E. coli</i>. Compounds <b>3</b> and <b>6</b> showed highest antioxidant activities with IC₅₀ values of 19.38 ± 0.64 and 32.81 ± 0.78 <i>μ</i>g/mL in DPPH, and 28.52 ± 1.08 and 27.31 ± 1.46 <i>μ</i>g/mL in H₂O₂, respectively. The isolated compounds also demonstrated considerable activity towards <i>S. aureus</i>. Among these compounds, compound <b>3</b> exhibited the highest activity (91.20 ± 0.12% and 9.14 ± 0.93 mm at 1.0 mg/mL) against this bacterium. The overall results suggest that the isolated compounds may be considered as potential sources of the bioactive agents to be used in the pharmacological, food, and other industries. Moreover, their high sensitivity against <i>S. aureus</i> may also support the use of <i>A. debrana</i> plant in the traditional medicine to treat wounds. Therefore, the isolated compounds are responsible for medicinal properties of this plant.</p>","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11093683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140921861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of In Vitro Antimicrobial, Cytotoxic, Thrombolytic, and Antiarthritic Property of Different Parts of Bari Orchid","authors":"Md. Samsur Rahaman, Md. Saifur Rahaman, Shah Md. Marzuk Hasnine, Salma Sultana, Md. Abdul Quaiyum Bhuiyan, Mohammad Shahriar Kabir, Md. Abdul Bari, Jahid M. M. Islam, Md. Ismail Hossain, Mubarak A. Khan","doi":"10.1155/2024/8148610","DOIUrl":"https://doi.org/10.1155/2024/8148610","url":null,"abstract":"Many different herbal extracts have historically been utilized to treat microbe-induced infections, injuries, cancer, thrombosis, and arthritis. The purpose of this study was to determine the antibacterial, cytotoxic, in vitro thrombolytic, and in vitro antiarthritic properties of ethanolic extracts of stem and seed of Bari orchid 1 (BO) plant. This orchid plant was developed by the Bangladesh Agriculture Research Institute (BARI) in Gazipur. Fourteen microbes were employed in the antimicrobial investigation, and samples of orchids were compared to ciprofloxacin as a reference. The BO/seed extract was found to possess more antibacterial activity. The lethality test of brine shrimps was used to assess the LC₅₀ values. The BO/stem extract exhibited a higher cytotoxicity potential, in comparison to the BO/seed extract. Two concentrations (1000 and 100 ppm) and two incubation times (24 hours and 1.5 hours) were used to assess the thrombolytic activity of the extracts. Regarding the thrombolytic effect, the BO/stem extract has demonstrated greater promise. Furthermore, the herbal extract’s antiarthritic activity was investigated at four different concentrations, and the results were evaluated in comparison with those of diclofenac sodium. When comparing BO/stem extract to other extracts, the greatest values for protein denaturation were obtained.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140837972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Homotherapy for Heteropathy: A Molecular Mechanism of Poria Sini Decoction for Treatment of Liver Cancer and Chronic Heart Failure","authors":"Zhe Zhao, Huiying Yue, Xiaohua Cui","doi":"10.1155/2024/9958258","DOIUrl":"https://doi.org/10.1155/2024/9958258","url":null,"abstract":"Poria sini decoction (PSD), a significant traditional Chinese herbal formula, is effective in liver cancer (LC) and chronic heart failure (CHF); however, little is known about its concurrent targeting mechanism. <i>Methods</i>. This study analyzed the potential molecular mechanism of PSD against the two distinct diseases using network pharmacology approaches, including multidatabase search, pharmacokinetic screening, network construction analysis, Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and molecular docking to elaborate the active components, signaling pathways, and potential mechanisms of PSD in the treatment of both LC and CHF. <i>Results</i>. A total of 155 active components and 193 potential targets in PSD were identified. Bioinformatics analysis revealed that quercetin, isorhamnetin, and naringenin, etc. may be potential candidate agents. TNF, AKT1, and IL6, etc. could become potential therapeutic targets. TNF-<i>α</i>, NF-<i>κ</i>B, PI3K-AKT, and TRP signaling pathways might play an important role in PSD against LC and CHF. Molecular docking results showed that most screened active compounds could embed itself into target proteins with a high binding affinity, and the hydrogen bonds number ≥3 indicated a more stable conformation of the compounds and target proteins. Overall, quercetin and isorhamnetin were the main active components, and TNF and AKT1 were the primary targets for PSD treatment of LC and CHF. <i>Conclusions</i>. This study illustrated that quercetin contained in PSD played an important role in the treatment of LC and CHF by acting on the key gene of TP53 and downregulating the PI3K-AKT signaling pathway.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140810683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential JAK2 Inhibitors from Selected Natural Compounds: A Promising Approach for Complementary Therapy in Cancer Patients","authors":"Samaneh Vaziri-Amjad, Reza Rahgosha, Amir Taherkhani","doi":"10.1155/2024/1114928","DOIUrl":"https://doi.org/10.1155/2024/1114928","url":null,"abstract":"<i>Background</i>. Janus-activated kinase 2 (JAK2) plays a pivotal role in numerous essential biological processes, including proliferation, apoptosis, and metastasis in human cells. Prior studies have indicated that inhibiting JAK2 could be a promising strategy to mitigate cell proliferation and induce apoptosis in tumor cells. <i>Objectives</i>. This study aimed to estimate the binding affinity of 79 herbal compounds, comprising 46 flavonoids, 21 anthraquinones, and 12 cinnamic acids, to the ATP-binding cleft of JAK2 to identify potential herbal inhibitors of JAK2. <i>Methods</i>. The binding affinities between ligands and JAK2 were calculated utilizing AutoDock 4.0 software in conjunction with the Cygwin environment. Cross-validation was conducted using the Schrödinger tool. Molecular dynamics simulations were employed to evaluate the stability of docked poses for the most significant JAK2 inhibitors. Furthermore, the Discovery Studio Visualizer tool was utilized to elucidate interactions between the top-ranked JAK2 inhibitors and residues within the JAK2 ATP-binding site. <i>Results</i>. Twelve flavonoids, two anthraquinones, and three cinnamic acids demonstrated substantial binding affinities to the protein kinase domain of the receptor, with a criterion of <svg height=\"14.1649pt\" style=\"vertical-align:-5.529pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 46.2943 14.1649\" width=\"46.2943pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,8.327,0)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,17.29,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,21.803,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,24.242,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,29.183,3.132)\"></path></g><g transform=\"matrix(.0091,0,0,-0.0091,33.988,3.132)\"><use xlink:href=\"#g190-106\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,36.426,3.132)\"><use xlink:href=\"#g190-111\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,41.331,3.132)\"></path></g></svg> &lt; −10 kcal/mol. Among the studied flavonoids, anthraquinones, and cinnamic acid derivatives, orientin, chlorogenic acid, and pulmatin emerged as the most potent JAK2 inhibitors, exhibiting <svg height=\"14.1649pt\" style=\"vertical-align:-5.529pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 46.2943 14.1649\" width=\"46.2943pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"><use xlink:href=\"#g113-133\"></use></g><g transform=\"matrix(.013,0,0,-0.013,8.327,0)\"><use xlink:href=\"#g113-72\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,17.29,3.132)\"><use xlink:href=\"#g190-99\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,21.803,3.132)\"><use xlink:href=\"#g190-106\"></use></g><g transform=\"matrix(.0091,0,0,-0.0091,24.242,3.132)\"><use xlink:href=\"#g190-111\"></use></g><g transform=\"matrix(.0091,0,0,-0.009","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140803815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trends in the Development of Acupuncture-Related Technologies Based on Patents in South Korea","authors":"Sung Min Lim, E. Go","doi":"10.1155/2024/2204071","DOIUrl":"https://doi.org/10.1155/2024/2204071","url":null,"abstract":"Background. Several studies have assessed the safety and efficacy of acupuncture, but none have reviewed patent trends related to acupuncture as an intervention. This study analyzed trends in patents for the development of acupuncture-related technologies in Korea. Methods. The Korea Intellectual Property Rights Information Service (KIPRIS) was searched for information on acupuncture-related patents registered until August 11, 2021, without any limit on the search period. Only acupuncture and electro-acupuncture were included in this review. The current status, contents, and technological characteristics of the relevant patents were analyzed systematically to identify the overall status of acupuncture-related patents. Results. Overall, 126 patents were identified from the KIPRIS database, of which 93 were unrelated to the research topic and were excluded. Finally, 33 patents were assessed. Interest in the development of acupuncture technology has increased steadily over the past decade. Patent applications reached their peak in 2018 with six instances, while patent registration peaked in 2019 with seven instances. The interval between the application and registration of an individual patent was 14.3 months (449 days). Twenty-two acupuncture-related patents were for acupuncture (66.6%) and 11 for electro-acupuncture (33.3%). The technical specifications for the patents included acupuncture devices (30.3%), acupuncture manufacturing methods and equipment (36.4%), and electro-acupuncture devices (33.3%). Conclusion. Acupuncture-related technology currently focuses on technological advancements for the safe and effective delivery of stimulation. The findings demonstrate trends toward new diverse and complex technological advancements for acupuncture devices, manufacturing methods and equipment, and electro-acupuncture devices.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140667060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hangeshashinto Inhibits Porphyromonas gingivalis Pathogen-Associated Molecular Patterns-Mediated IL-6 and IL-8 Production through Toll-Like Receptors in CAL27 Cells","authors":"Hourei Oh, Yoshimasa Makita, Kazuya Masuno, Yasuhiro Imamura","doi":"10.1155/2024/9866670","DOIUrl":"https://doi.org/10.1155/2024/9866670","url":null,"abstract":"While previous reports have established the anti-inflammatory effects of hangeshashinto, the intracellular signal transduction pathways involved have yet to be elucidated. We aim to employ an experimental system using oral cancer cells to assess the impact of hangeshashinto on intracellular signal transduction pathways in response to stimulation by <i>Porphyromonas gingivalis</i> pathogen-associated molecular patterns (PAMP). Hangeshashinto demonstrated the ability to inhibit the production of interleukin (IL)-6 and IL-8 induced by <i>P. gingivalis</i> PAMP. Furthermore, hangeshashinto suppressed the activation of the IL-6 promoter stimulated by PAMP. Hangeshashinto, like Toll-like receptor (TLR) signaling inhibitors (resatorvid and C29) and an immunosuppressant (dexamethasone), exhibited the ability to suppress TLR-mediated activation of the transcription factor nuclear factor-<i>κ</i>B (NF-<i>κ</i>B) in response to PAMP stimulation. This study suggests that the anti-inflammatory effects of hangeshashinto may be attributed to the inhibition of TLR signal transduction pathways including NF-<i>κ</i>B activation, thereby suppressing NF-<i>κ</i>B-dependent gene expression.","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140612305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the Protoscolicidal Activity of Humulus lupulus Methanolic Extracts on Echinococcus granulosus Sensu Stricto","authors":"Clara María Albani, Azucena Iglesias, Adriana Albanese, Giselle Fuentes, Dalila Orallo, Matías Maggi, María Celina Elissondo","doi":"10.1155/2024/6251666","DOIUrl":"https://doi.org/10.1155/2024/6251666","url":null,"abstract":"The larval stage of the parasite <i>Echinococcus granulosus sensu lato</i> (s.l) is responsible for cystic echinococcosis (CE), a long-term infection affecting humans and animals worldwide, and constitutes a serious public health concern. If left untreated, CE can cause serious damage to multiple organs, especially the liver and lungs. Regarding the treatment, in the last few years, the use of pharmacological treatment has increased, suggesting that in the future, drug therapy may replace surgery for uncomplicated cysts. However, the only available anthelmintic drug to treat this infection is the albendazole, which has an efficacy that does not exceed 50%. On the basis of the above-mentioned evidence, new and improved alternative treatments are urgently needed. The use of natural products and their active fractions and components holds great promise as a valuable resource for the development of novel and effective therapies. Hop (<i>Humulus lupulus</i> L.) is a bittering agent in the brewing industry for which the sedative, digestive, anti-inflammatory, and antimicrobial effects have been reported. The purpose of this study was to assess the <i>in vitro</i> efficacy of methanolic extracts from the leaves of hop varieties against <i>E. granulosus sensu stricto</i> (s.s) protoscoleces. Varieties Mapuche and Victoria caused a stronger protoscolicidal effect compared to the Bullion, Cascade, and Traful varieties (<span><svg height=\"9.2729pt\" style=\"vertical-align:-0.6370001pt\" version=\"1.1\" viewbox=\"-0.0498162 -8.6359 19.289 9.2729\" width=\"19.289pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,0,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,11.658,0)\"></path></g></svg><span></span><span><svg height=\"9.2729pt\" style=\"vertical-align:-0.6370001pt\" version=\"1.1\" viewbox=\"22.8711838 -8.6359 21.918 9.2729\" width=\"21.918pt\" xmlns=\"http://www.w3.org/2000/svg\" xmlns:xlink=\"http://www.w3.org/1999/xlink\"><g transform=\"matrix(.013,0,0,-0.013,22.921,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,29.161,0)\"></path></g><g transform=\"matrix(.013,0,0,-0.013,32.125,0)\"><use xlink:href=\"#g113-49\"></use></g><g transform=\"matrix(.013,0,0,-0.013,38.365,0)\"></path></g></svg>),</span></span> coinciding with their highest content of flavonoids, total polyphenols, and saponins. The viability of protoscoleces treated with the varieties Mapuche and Victoria decreased to approximately 50% at days 5 y 8, respectively, showing alterations such as soma contraction and impaired microtriches. After 18 days of treatment with both varieties, protoscoleces were completely altered both structurally and ultrastructurally. In conclusion, the methanolic extracts of the <i>H. lupulus</i> varieties Mapuche and Victoria demonstrated a marked <i>in vitro</i> effect against <i>E. granulosus</i> s.s. protoscoleces. The beer-making industry exclusively uses hop cones, leaving behind large amounts of hop leaves as an a","PeriodicalId":12236,"journal":{"name":"Evidence-based Complementary and Alternative Medicine","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140580786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}