Evidence-based dentistry最新文献

{"title":"Deep convolutional neural networks for early detection of interproximal caries using bitewing radiographs: A systematic review.","authors":"Soundar Ida Mahizha, Joseph Annrose, Jeyebalaji Mano Christaine Angelo, Israel Domilin Shyni, G Valanthan Veda Giri","doi":"10.1038/s41432-025-01134-7","DOIUrl":"10.1038/s41432-025-01134-7","url":null,"abstract":"<p><strong>Objectives: </strong>To thoroughly review Deep Convolutional Neural Networks for detecting interproximal caries with bitewing radiographs.</p><p><strong>Data: </strong>Data was collected from studies that utilized Deep Convolutional Neural Networks (DCNN) focused on the analysis of bitewing radiographs taken with intraoral X-ray units.</p><p><strong>Sources: </strong>A comprehensive literature search was conducted across various scholarly databases including Google Scholar, MDPI, PubMed, ResearchGate, ScienceDirect, and IEEE Xplore, encompassing 2014 to 2024. The risk of bias assessment utilized the current version of the Quality Assessment Tool for Diagnostic Accuracy Studies (QUADAS-2).</p><p><strong>Study selection: </strong>After reviewing 291 articles, 10 studies met the criteria and were analyzed. All 10 studies used bitewing radiographs, focusing on deep learning tasks such as segmentation, classification, and detection. The sample sizes varied widely from 112 to 3,989 participants. Convolutional neural networks (CNNs) were the most commonly used model. According to the QUADAS-2 assessment, only 40% of the studies included in this review were found to have a low risk of bias in the reference standard domain.</p><p><strong>Clinical significance: </strong>A Deep Convolutional Neural Networks based caries detection system helps in the early identification of caries by analyzing bitewing radiographs and reduces diagnostic errors. By identifying early-stage lesions, patients can undergo minimally invasive treatments instead of more complex procedures, thereby improving patient outcomes in dental care.</p><p><strong>Conclusion: </strong>This systematic review provides an overview of various studies that utilize deep learning models to identify interproximal caries lesions in bitewing radiographs. It highlights the efficacy of YOLOv8 in detecting interproximal caries from bitewing radiographs compared to other Deep CNN models.</p>","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Grading the strength and certainty of the scientific evidence of the bidirectional association between periodontitis and noncommunicable diseases: an umbrella review.","authors":"Carolina Rodríguez-Medina, Sandra Amaya Sánchez, Adolfo Contreras, Javier Enrique Botero","doi":"10.1038/s41432-025-01132-9","DOIUrl":"https://doi.org/10.1038/s41432-025-01132-9","url":null,"abstract":"<p><strong>Objective: </strong>Periodontitis and various noncommunicable diseases (NCDs) have been proposed to have a bidirectional relationship. The purpose of this umbrella review is (1) to synthesize the evidence and (2) to grade the strength and certainty of the scientific evidence regarding the bidirectional association between periodontitis and NCDs.</p><p><strong>Data sources: </strong>Electronic databases were systematically searched from January 2021 and July 2024; MEDLINE (via PubMed), Embase and SciELO.</p><p><strong>Data selection and extraction: </strong>Potential epidemiologic systematic reviews with meta-analysis that studied the bidirectional association between periodontitis and NCDs were identified by two independent reviewers and filtered by title and abstract according to the selection criteria. The strength and the quality and certainty of the evidence was assessed according to the Grading of Recommendations, Assessment, Development and Evaluations (GRADE) guide. 561,554 potential results were identified. After removing duplicates and excluding records deemed ineligible by automated filters, 450 results were screened by title and abstract. This process led to 41 records being appraised in full-text. Of these, 17 were further excluded leaving a total of 24 systematic reviews that met the inclusion criteria.</p><p><strong>Data synthesis: </strong>24 systematic reviews with a total of 32 NCDs were appraised and consolidated. Risk of bias assessment indicated that 21 systematic reviews (87.5%) demonstrated low bias (high quality), 2 had medium bias, and 1 exhibited high bias (low quality). Key issues identified included the formulation of explicit research questions, critical appraisal, data extraction, and publication bias. The association between periodontitis and NCDs was strong in 1 systematic review, moderate in 8, weak in 10 and absent in 7 systematic reviews. The strength of the association between NCDs and periodontitis was moderate in 6 systematic reviews and weak in 3 systematic reviews. The size of the reported effect (odds ratio/risk ratio/hazard ratio) was broader with increasing strength. Although data supports the association between periodontitis and some NCDs, and to a lesser extent between some NCDs and periodontitis, the certainty of the evidence was classified as low to very low.</p><p><strong>Conclusions: </strong>There is some data that, with varying degrees of association and low to very low certainty, provide evidence that periodontitis may be a potential risk factor for some NCDs and vice versa.</p>","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can caries in anterior teeth of toddlers predict severe caries in posterior teeth?","authors":"Meenakshi Lall","doi":"10.1038/s41432-025-01128-5","DOIUrl":"10.1038/s41432-025-01128-5","url":null,"abstract":"Tavares B S, Bendo C B, Fernandes I B, Coelho V S, Ramos-Jorge M L, Ramos-Jorge J Dental caries in anterior teeth of babies can predict the occurrence of severe dental caries in posterior teeth: a 3-year cohort study. Eur Arch Paediatr Dent 2024; https://doi.org/10.1007/s40368-024-00968-6 . A three-year study of 1–2 years old toddlers to check if the dental caries of primary anterior teeth is a precursor to them developing severe caries on their primary molars. A cohort of 99 children, between the ages of 1–2 years old, reviewed for dental caries. This study looked at the toddlers at the baseline and then reviewed them after three years. Primary independent variable in this study was carious anterior tooth. Consideration given to independent variables like economic status, primary carers’ education, oral hygiene (including nighttime routine), history of tooth ache, and sucrose intake. Differences between the presence and absence of severe dental caries in posterior teeth according to independent variables were evaluated using the chi-square test. Unadjusted and adjusted relative risks for the association between independent variables and the incidence of severe caries in posterior teeth were estimated using Poisson regression models with robust variance. There is threefold increased risk of developing severe dental caries in posterior teeth for the children who had caries in anterior teeth at the baseline (RR 3.33; 95% CI 2.06–5.37). This high-risk group belonged to low-income families (RR 2.75; 95% CI 1.40–5.39), who did not perform nighttime oral hygiene (RR 1.76; 95% CI 1.10–2.80), and had a higher frequency of sucrose intake (RR 4.07; 95% CI 2.03–8.19), at the baseline and at follow-up. The presence of dental caries in the primary anterior teeth of 1 to 2-year-old toddlers can have a higher incidence of severe caries in posterior teeth. Other factors e.g. low economic status, absence of nighttime oral hygiene, high sucrose intake both at baseline and follow-up were considerable risk factors for the presence of severe dental caries in the primary posterior teeth.","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":"26 1","pages":"40-41"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143624014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iranian early childhood dental caries: a comprehensive systematic review and meta-analysis of prevalence and associated risk factors","authors":"Razieh Jabbarian,&nbsp;Mehdi Ranjbaran,&nbsp;Aida Mokhlesi,&nbsp;Samin Hosseini","doi":"10.1038/s41432-024-01078-4","DOIUrl":"10.1038/s41432-024-01078-4","url":null,"abstract":"Early childhood dental caries (ECC) is a prevalent dental condition affecting infants and young children, characterized by one or more carious lesions in primary teeth. ECC risk factors include malnutrition, genetic predisposition, specific dietary habits, lack of fluoride, and prolonged bottle feeding. A comprehensive prevalence estimate is crucial for understanding the extent of dental caries in the population. So, this study aimed to determine the prevalence of ECC and its associated risk factors in Iranian children. A comprehensive literature search was conducted in Scopus, Web of Science, PubMed, Google Scholar, ACECR Scientific Information Database ( www.ACECRScientific Information Database.ir ), and Magiran research databases. Eligible English and Persian studies published between 2006 and 2024 were reviewed and analyzed. The studies on ECC prevalence were identified and included in the meta-analysis. Random effect models were utilized to calculate the pooled prevalence, and meta-regression and sensitivity analyses were conducted to assess sources of heterogeneity in the systematic review of risk factors. The meta-analysis encompassed thirty eligible studies, providing the prevalence of ECC in Iran, with an estimated prevalence of 61.7%. Meta-regression analysis indicated a non-statistically significant increase in ECC prevalence with age and a substantial slight decrease over time. Additionally, 68 Studies evaluated categories such as age, gender, sociodemographic factors, microbial flora, oral hygiene, breast/bottle feeding, and dietary. Significant ECC-related factors identified included parents’ education and occupation, breastfeeding, and oral hygiene. The overall prevalence of ECC in Iran was estimated at 61.7%, with notable heterogeneity. Most of the studies extensively evaluated sociodemographic factors as risk factors.","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":"26 1","pages":"66-66"},"PeriodicalIF":0.0,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Osteopontin as a prognostic biomarker in head and neck cancer- a systematic review and meta-analysis.","authors":"Nadisha Piyarathne, Kalani Hettiarachchi, Monal Yuwanati, Gowri Sivaramakrishnan, Anand Ramanathan, Ruwan Jayasinghe, Sanaa Chala, Akhilanand Chaurasia","doi":"10.1038/s41432-025-01133-8","DOIUrl":"https://doi.org/10.1038/s41432-025-01133-8","url":null,"abstract":"<p><strong>Background: </strong>Osteopontin (OPN) is linked to cancer progression, metastasis, and treatment resistance in head and neck cancer (HNC). This meta-analysis evaluated OPN as a prognostic biomarker in HNC.</p><p><strong>Method: </strong>A comprehensive search was conducted for studies published up to December 2023, including English papers on HNC analyzing OPN expression. Data extraction, quality assessment, and quantitative analysis were performed using fixed and random effect models with 95% CI. Heterogeneity and publication bias were assessed with I2 and Egger's regression test.</p><p><strong>Results: </strong>Fifty-one studies were included. OPN expression was significantly elevated in tissue and plasma in HNC compared to control (SMD 0.98; 95% CI 0.47-1.49; I2 = 13%; p < 0.00). High plasma OPN predicted poor survival (HR: 2.00; I2 = 64%; P = 0.03), as did high tissue OPN (hazard ratio: 2.71, 95% confidence interval = 1.51-4.87; I2 = 49.4%, p > 0.05). Elevated plasma OPN correlated with smoking, poorly differentiated neoplasms, larger tumors, advanced stage, and lymph node metastasis. Positive tissue OPN was associated with nodal involvement, advanced stage, male gender, and smoking.</p><p><strong>Conclusion: </strong>OPN is a robust prognostic biomarker in HNC, indicating tumor aggressiveness and poor prognostic outcomes. Standardized measurement protocols and further validation in prospective studies are necessary.</p>","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143614100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What about anterior endocrowns?","authors":"Madeline Archer","doi":"10.1038/s41432-025-01126-7","DOIUrl":"10.1038/s41432-025-01126-7","url":null,"abstract":"Fehrenbach J, de Soares J L S, do Nascimento Foly J C S, Miotti L L, Münchow E A Mechanical performance of endocrown restorations in anterior teeth: A systematic review and network meta-analysis. Dent Mater 2025; https://doi.org/10.1016/j.dental.2024.10.012 . A systematic review and network meta-analysis (NMA) of anterior endocrown mechanical performance compared to conventional crowns on endodontically treated teeth (ETT). An online search of major databases was conducted and relevant studies were selected. Data was extracted and meta-analyses performed comparing mechanical outcomes of anterior endocrowns to other crown systems. Endocrowns on the anterior dentition can be considered if mechanical fracture is a concern, however more research needs to be done before definitive case selection. Two independent reviewers searched six electronic databases (PubMed, Scopus, Web of Science, Embase, SciELO and LILACS) using compounded terms like ETT and anterior and endocrown along with their variates. Manual searches were also done from the reference list of chosen studies. Following the search titles were put through Rayyan, an AI-powered systematic reviewer to remove duplicates. The reviewers then screened 25 random titles and abstracts independently and repeated this with a third reviewer. Inclusion criteria were studies examining anterior ETT and endocrown compared with a different restoration. Exclusion criteria were non-experimental studies, theses, languages other than English Spanish or Portuguese and studies not evaluating mechanical performance. From this 24 studies were read in full and 11 were selected for the review. Bias risk assessment was done by two reviewers using the RoBDEMAT tool for in vitro studies and ROBFEAD tool for Finite Element Analysis (FEA) studies. The studies are scored as low, med, or high risk according to each tool’s domains. Two reviewers extracted the data onto Microsoft Excel including authors name and year of publication, tooth type, endocrown depth, distance between the CEJ and finishing line,surface treatment, luting agent, thermocycling method, etc. Data synthesis and analysis was performed by MetaInsight V4 4.0.0 and two independent NMA’s were made. The first NMA used load-to-fracture data and a mean difference outcome while the second used irreparable fracture data and a risk ratio outcome. Both used a 95% credible interval(Crl). Comparisons were evaluated using Surface Under the Cumulative ranking Curve (SUCRA), where a value closer to 100% indicates greater resistance to fracture. Eight studies used in vitro experiments, most of them investigating load-to-fracture and failure mode of fractured restorations and one study the pull-out bond strength. The remaining three studies used FEA models and examined von mises criterion by quantifying a material’s fracture under stress. The first NMA considered load-to-fracture data and examined five studies. The probabilistic analysis showed teeth restored with a Glass Fi","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":"26 1","pages":"10-11"},"PeriodicalIF":0.0,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial resistance genes in the oral microbiome","authors":"Manas Dave,&nbsp;Rajpal Tattar","doi":"10.1038/s41432-025-01120-z","DOIUrl":"10.1038/s41432-025-01120-z","url":null,"abstract":"Sukumar S, Rahmanyar Z, El Jurf H Q et al. Mapping the oral resistome: a systematic review. J Med Microbiol 2024; https://doi.org/10.1099/jmm.0.001866 . This systematic review, without meta-analysis, aimed to map the oral resistome by analysing clinical studies that detected bacterial antimicrobial resistance genes (ARGs) in the oral cavity using molecular techniques. The researchers used Medline, Embase, Web of Science, CINAHL and Scopus databases from January 2015 to August 2023. This systematic review included cross-sectional or longitudinal clinical studies that detected ARGs using molecular techniques; specifically polymerase chain reaction (PCR) or next-generation sequencing (NGS) metagenomics&nbsp;for samples from the oral cavity (saliva, gingival biofilm, pulp, or oral mucosa). Studies were excluded if they were in vitro or animal studies, literature reviews and not focused on ARG detection. Five reviewers independently screened titles and abstracts based on inclusion criteria. Full-text reports were then independently assessed for eligibility by three reviewers. Extracted data encompassed publication details, sample size, country, molecular methods used, number of ARGs detected, participants’ health status, antibiotic exposure, and sample location within the oral cavity. Out of 580 initially identified studies, 15 met the inclusion criteria. These studies, published between 2015 and 2023 from 12 different countries, employed either PCR (n = 10) or NGS metagenomics (n = 5) to detect ARGs from a pool of 1486 participants (1 study did not report on the number of participants). PCR-based studies identified an average of 7 ARGs (range 1–20), while NGS studies identified an average of 34 ARGs (range 7–70). In total, 159 unique ARGs conferring resistance to 22 antibiotic classes were identified across six regions of the oral cavity. The supragingival biofilm and saliva exhibited the highest richness of ARGs, defined by the number of unique ARGs detected. Genes conferring resistance to 19 antibiotic classes were present in the supragingival biofilm. Notably, 49 ARGs, including tetracycline and macrolide resistance genes, were found across all sampled locations, indicating a widespread distribution within the oral cavity. Thirteen studies reported on bacterial species associated with ARGs. NGS studies identified a mean of 65 ARG-carrying bacterial species, compared to a mean of 4 species in PCR studies. Specifically, 25 ARG-carrying species were identified in PCR studies, while NGS studies identified 177 species. Four studies reported ARGs associated with streptococcal species implicated in distant-site infections such as infective endocarditis. ESKAPE pathogens (group of highly virulent multidrug-resistant bacteria) were detected with ARGs in various oral sites using both PCR and NGS methods. Comparisons between healthy and diseased states revealed that a healthy oral microbiome harbours a more diverse resistome at the antibiotic class level. Th","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":"26 1","pages":"42-43"},"PeriodicalIF":0.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41432-025-01120-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Should we prescribe systemic antibiotics alongside periodontal therapy for diabetic patients?","authors":"Sophie Rimmer,&nbsp;Marianne Dobson","doi":"10.1038/s41432-024-01096-2","DOIUrl":"10.1038/s41432-024-01096-2","url":null,"abstract":"This single-centre, short-term randomized control trial (RCT) assesses the effect of systemic amoxicillin and metronidazole as an adjunct to SRP in patients with severe periodontitis and type 2 diabetes mellitus (T2DM) on glycaemic control, over a 3-month period. The authors hypothesize that adjunctive systemic antibiotics will decrease systemic inflammation and subsequently improve glucose control. Baseline periodontal examinations and haematological tests were performed for all subjects. Subsequently, subgingival scaling and root planning (SRP) procedures were performed by a single periodontist for both the test and control groups. The test group received 500 mg amoxicillin and 200 mg metronidazole TDS for 7 days in the same week of the SRP procedure. Three months later, periodontal examinations were repeated by blinded examiners and haematological tests were repeated. Maintenance periodontal therapy was provided at this time. A convenience sample of patients visiting the periodontal department between 2016-2022 was used. Participants included were aged 40–75; diagnosed with T2DM for &gt;2 years; HbA1c of 6.5–10%, had stable medication regimens and &gt;15 remaining teeth. Participants had a periodontal diagnosis of generalized severe chronic periodontitis or stage III-IV generalized periodontitis based on the 1999 and 2018 classifications respectively. Exclusion criteria related to coexisting inflammatory or infectious diseases (e.g. malignancy, coronary heart disease or hepatitis); adjustment in diet or glycaemic control strategy; severe diabetic complications; pregnancy or lactation; allergies to amoxicillin or metronidazole; periodontal treatment or antibiotics within 3 months prior; smoking and alcohol abuse. The primary clinical outcome was a change in HbA1c. Secondary outcomes included the effect on periodontal parameters and haematological markers of inflammation. Periodontal parameters measured were probing depth (PD), bleeding index (BI), Plaque index (PI), periodontally inflamed surface area (PISA), and clinical attachment level (CAL). Haematological parameters were glycated haemoglobin (HbA1c), fasting blood glucose (FBG), neutrophil-to-lymphocyte ratio (NLR), and white blood cell (WBC) count. For data analysis, poorly controlled T2DM was defined as baseline HbA1c &gt; 7.5%. Statistical significance was defined as p &lt; 0.05. Analysis within and between the two groups was analysed with Student’s t-tests when data followed a normal distribution and the Mann-Whitney U test for non-normally distributed data. Chi-square testing was used to compare categorical variables. Uni- and multivariable binary logistic regression was employed to analyze the effectors related to HbA1c decrease. Intention-to-treat analysis was carried out for all enroled participants. 49 participants were enroled in the study; 23 were assigned to the SRP-only group and 26 to the SRP + antibiotics group. Nine participants were lost to follow-up. HbA1c levels decrease","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":"26 1","pages":"48-49"},"PeriodicalIF":0.0,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143566609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Change and opportunity","authors":"Elizabeth Kay","doi":"10.1038/s41432-025-01124-9","DOIUrl":"10.1038/s41432-025-01124-9","url":null,"abstract":"","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":"26 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.nature.com/articles/s41432-025-01124-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of pulp tissue genetic profile in orthodontic tooth movement: a needle in a haystack?","authors":"Soumya Narayani Thirumoorthy,&nbsp;Darshit Shah,&nbsp;Saumiya Gopal","doi":"10.1038/s41432-025-01131-w","DOIUrl":"10.1038/s41432-025-01131-w","url":null,"abstract":"Zhao Z, Attanasio C, Zong C, Pedano M S, Cadenas de Llano-Pérula M. How does orthodontic tooth movement influence the dental pulp? RNA-sequencing on human premolars. Int Endod J 2024; 57: 1783–1801. Sample selection&nbsp;- The study sample consisted of healthy individuals requiring extraction of maxillary or mandibular premolars for fixed appliance orthodontic treatment. Exclusion criteria included systemic diseases, a history of smoking, antibiotic or analgesic use within the past three months, periodontal probing depths greater than 3 mm, alveolar bone resorption, and premolars with prior endodontic treatment. Participants were randomly divided into 3 groups:&nbsp;control, 7-day, and 28-day groups. Extractions were performed prior to orthodontic treatment, and 7 days and 28 days after application of orthodontic forces (OF) in the control, 7-day and 28-day group respectively. The applied orthodontic forces were in the range of 50–150 g. RNA extraction from pulp tissues was performed using the RNeasy mini kit. After total RNA extraction, a library was prepared, and RNA sequencing was conducted to examine and compare the genetic data among the groups within each jaw. Differentially expressed genes (DEGs) were identified in the 7-day and 28-day groups, followed by functional enrichment analysis to understand their biological significance. Furthermore, protein-protein interaction network analysis was conducted to investigate the complex network of protein interactions and locate key genes involved in orthodontic tooth movement. Immune response, hypoxia, DNA damage, and epigenetic regulation were the pulpal reactions reflected after 7 days of OF. Whereas, at 28 days, processes such as cell adhesion, migration, organization, and tissue repair became prominent. Pulp tissues in the maxilla and mandible responded differently to orthodontic force. The maxilla showed minimal changes, primarily involving immune response at 7 days and tissue repair at 28 days. In contrast, the mandible exhibited significant DNA damage and epigenetic regulation at 7 days, with a return to its baseline condition by 28 days. Dental pulp demonstrated different reactions at 7 and 28 days, with maxillary and mandibular pulp tissues presenting distinct responses. The study provided knowledge about gene regulatory mechanisms modulating pulp tissue response to OF.","PeriodicalId":12234,"journal":{"name":"Evidence-based dentistry","volume":"26 1","pages":"61-62"},"PeriodicalIF":0.0,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143540759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}