FEMS immunology and medical microbiology最新文献

{"title":"Subject Index Volume 44","authors":"","doi":"10.1016/S0928-8244(05)00102-1","DOIUrl":"https://doi.org/10.1016/S0928-8244(05)00102-1","url":null,"abstract":"","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 3","pages":"Pages 327-333"},"PeriodicalIF":0.0,"publicationDate":"2005-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0928-8244(05)00102-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137131949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The humoral immuneresponse to Helicobacter pylori infection in children with gastrointestinal symptoms","authors":"Daiva Janulaityte-Gunther ,&nbsp;Rūta Kucinskiene ,&nbsp;Limas Kupcinskas ,&nbsp;Alvydas Pavilonis ,&nbsp;Liutauras Labanauskas ,&nbsp;Arvydas Cizauskas ,&nbsp;Uwe Schmidt ,&nbsp;Torkel Wadström ,&nbsp;Leif Percival Andersen","doi":"10.1016/j.femsim.2005.02.005","DOIUrl":"10.1016/j.femsim.2005.02.005","url":null,"abstract":"<div><p>The prevalence of <span><em>Helicobacter </em><em>pylori</em></span> is high in Eastern Europe. The purpose of this study was to estimate the prevalence of <em>H. pylori</em> in symptomatic Lithuanian children and to identify the infection by clinicopathological and serological analyses.</p><p><span><span>One hundred sixteen symptomatic children (age 8–16) with gastritis<span> and duodenal ulcer were included. Biopsies were histologically assessed according to the Sydney-System. Serum </span></span>IgG antibodies against </span><em>H. pylori</em><span><span> were detected by an enzyme-linked immunosorbent assay (ELISA), using low molecular mass antigen. The western blot<span> technique was used to detect serum antibodies against the cytotoxin-associated protein (CagA) using whole </span></span>cell antigen.</span></p><p>Histologically the prevalence of <em>H. pylori</em> infection was 79% and not influenced by demographic factors. Mucosal inflammation and atrophy were associated with a <em>H. pylori</em><span> infection. Intestinal metaplasia was found in eight children, suggesting early </span><em>H. pylori</em> acquisition in life.</p><p>Increased levels of IgG antibodies were detected in 57% of children. The prevalence of IgG antibodies was significantly higher in patients with duodenal ulcer compared to children with gastritis. Forty-four (67%) <em>H. pylori</em><span>-seropositive children had antibodies against CagA. Low molecular weight-ELISA and whole cell-western blot results were significantly associated with histopathology, the presence of duodenal ulcer and the CagA status. A high number of false seronegative cases were due to poor immunological responses in children and poor locally validated tests.</span></p><p>The prevalence of <em>H. pylori</em> infection in Lithuanian children is higher compared to Western Europe. The infection is acquired in early life. Diagnosing <em>H. pylori</em><span> infection, serology is helpful, but endoscopy/histology remains as gold standard.</span></p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 2","pages":"Pages 205-212"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.02.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25087291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a new sialic acid-binding protein in Helicobacter pylori","authors":"Hayley J. Bennett,&nbsp;Ian S. Roberts","doi":"10.1016/j.femsim.2004.11.008","DOIUrl":"10.1016/j.femsim.2004.11.008","url":null,"abstract":"<div><p><span>A novel sialic acid-specific lectin has been isolated from </span><span><span>Helicobacter pylori</span></span><span><span> lysate using fetuin–agarose affinity chromatography followed by cleavage of the α(2,3) and α(2,6) linkages of sialic acids using </span>neuraminidase<span>. The protein had a molecular weight of 17.5 kDa on sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (PAGE) and was identified by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry to be protein of unknown function with gene number HP0721. Recombinant HP0721 was shown to bind to fetuin–agarose and sialic acid-containing glycosphingolipids<span> on thin-layer plates suggesting this protein may represent another sialic acid-specific adhesin of </span></span></span><em>H. pylori</em>. A <em>H. pylori</em> mutant defective for HP0721 was generated and its ability to bind to human AGS cells assayed.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 2","pages":"Pages 163-169"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2004.11.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25089017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastric inflammatory markers and interleukins in patients with functional dyspepsia, with and without Helicobacter pylori infection","authors":"Leif P. Andersen ,&nbsp;Susanne Holck ,&nbsp;Daiva Janulaityte-Günther ,&nbsp;Limas Kupcinskas ,&nbsp;Gediminas Kiudelis ,&nbsp;Laimas Jonaitis ,&nbsp;Dainius Janciauskas ,&nbsp;Peter Holck ,&nbsp;Mads Bennedsen ,&nbsp;Henrik Permin ,&nbsp;Svend Norn ,&nbsp;Torkel Wadström","doi":"10.1016/j.femsim.2004.10.022","DOIUrl":"10.1016/j.femsim.2004.10.022","url":null,"abstract":"<div><p><span><em>Helicobacter </em><em>pylori</em></span><span><span> is the most important cause of gastritis<span>, peptic ulcers and the development of gastric cancer. The chronic active inflammation is dominated by neutrophils, macrophages, lymphocytes and plasma cells. Several </span></span>interleukins<span><span><span> (IL-8, IL-10 and IFN-γ) are involved in the inflammatory process in the gastric mucosa. The aim of this study was to investigate the gastric inflammation in patients with functional dyspepsia. Fifty-three consecutive patients were included and antral biopsies were obtained for histology, culture and immunohistochemistry. The sections were examined for the interleukins IL-4, IL-6, IL-8, IL-10 and IFN-γ as well as for the cell markers CD4, CD8, </span>CD14, </span>Cd19<span>, CD25 and CD30.</span></span></span></p><p>Only CD4 and CD19 were significantly increased in patients with increased gastric inflammation and increased density of <em>H. pylori</em>. However, several of the examined markers (IFN-γ, IL-8, IL-10 and CD14) showed a non-significant trend to be increased in patients with extensive gastric inflammation and high density of <em>H. pylori</em>. Therefore, an arbitrary index (IM₁₁) for all the 11 immunological markers was made as an average value for each of the four morphological groups. For the four morphologically different groups of patients the values were 0.49, 0.77, 0.86 and 1.25, respectively. Significant increases in the index from none to moderate antral inflammation as well as the density of <em>H. pylori</em> were found (<em>p</em> <!-->&lt;<!--> <!-->0.001). By using an index of inflammatory markers trends can be summarized and thereby significant which may be of importance when gastric inflammation is investigated in children and patients with functional dyspepsia.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 2","pages":"Pages 233-238"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2004.10.022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25087295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The presence of the cag pathogenicity island is associated with increased superoxide anion radical scavenging activity by Helicobacter pylori","authors":"Jacob J. Briedé ,&nbsp;Raymond G.J. Pot ,&nbsp;Ernst J. Kuipers ,&nbsp;Arnoud H.M. van Vliet ,&nbsp;Jos C.S. Kleinjans ,&nbsp;Johannes G. Kusters","doi":"10.1016/j.femsim.2004.10.018","DOIUrl":"10.1016/j.femsim.2004.10.018","url":null,"abstract":"<div><p>Reactive oxygen species (ROS) generated by <span><em>Helicobacter </em><em>pylori</em></span><span> infection have been suggested to be important factors in induction of gastric malignancies. Utilizing electron spin resonance spectrometry, </span><em>H. pylori</em>-dependent radical formation and hydroxyl- and superoxide-anion radical scavenging activity was investigated. In contrast to previous reports, we found that <em>H. pylori</em> does not produce ROS, but displays superoxide scavenging activity. This scavenging activity was increased in <em>cag</em>-positive <em>H. pylori</em> strains when compared to strains lacking an intact <em>cag</em><span> pathogenicity island<span>, and was dependent on enzyme activity. We hypothesize that the increased scavenging activity of </span></span><em>cag</em>-positive <em>H. pylori</em> strains is an adaptation to the increased inflammatory response associated with the <em>cag</em>-positive genotype of <em>H. pylori</em>.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 2","pages":"Pages 227-232"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2004.10.018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25087294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of inflammation in gastrointestinal tract in aetiology and pathogenesis of idiopathic parkinsonism","authors":"Clive Weller ,&nbsp;Norman Oxlade ,&nbsp;Sylvia M. Dobbs ,&nbsp;R. John Dobbs ,&nbsp;André Charlett ,&nbsp;Ingvar T. Bjarnason","doi":"10.1016/j.femsim.2005.01.011","DOIUrl":"10.1016/j.femsim.2005.01.011","url":null,"abstract":"<div><p><span><span>Idiopathic parkinsonism (IP) is a common disorder, conventionally regarded as neurodegenerative. Its cardinal features, poverty and slowness of movement, muscle rigidity, </span>postural abnormality<span><span> and a characteristic tremor, are associated with loss of dopaminergic<span><span> neurones in the substantia nigra of the brain. </span>Genetic factors explain only a minority of cases, and a common toxic environmental insult remains elusive. We propose that IP is a </span></span>systemic disorder resulting from a ubiquitous peripheral infection, and that only the tip of the iceberg comes to diagnosis. There is evidence for inflammatory/immune activation peripherally and in the brain. We have used statistical modelling to explore links with non-specific and specific systemic markers of inflammation/infection in IP probands, and explore whether their partners and siblings have a frank or pre-presentation parkinsonian state. Critical to this approach is continuous objective measures of the facets of IP. Hypotheses on causality and mechanism are based on the statistical models. There is pathological and clinical evidence for direct involvement of the gastrointestinal tract in IP. The candidacy of </span></span><em>Helicobacter pylori</em><span> infection as a trigger event or driving infection is relatively high. We have found that eliminating infection in late parkinsonism with cachexia<span>, a stage usually considered intractable, can result in a U-turn. However, eradication therapy<span> may not provide a complete solution. Persistence of antibody against cytotoxin-associated antigen (CagA), increases the predicted probability of being labelled as having parkinsonism. Evidence for autoimmunity and immunocompromise is used to build schemes for the natural history. We conclude that current classifications of neuropsychiatric disease may not prove the best with respect to defining sub-clinical disease, prophylaxis or halting progression.</span></span></span></p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 2","pages":"Pages 129-135"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.01.011","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25089012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of the Helicobacter pylori adherence factor BabA with duodenal ulcer disease in four European countries","authors":"Farzad O. Olfat ,&nbsp;Quing Zheng ,&nbsp;Monica Oleastro ,&nbsp;Petra Voland ,&nbsp;Thomas Borén ,&nbsp;Riita Karttunen ,&nbsp;Lars Engstrand ,&nbsp;Roland Rad ,&nbsp;Christian Prinz ,&nbsp;Markus Gerhard","doi":"10.1016/j.femsim.2004.10.010","DOIUrl":"10.1016/j.femsim.2004.10.010","url":null,"abstract":"<div><p><span><span>Helicobacter pylori</span></span> strains harboring the <em>vacAs1</em>, <em>cagA</em> and <em>babA2</em><span> have been associated with ulcer disease (UD). We compared the prevalence of these different genotypes and adhesive properties in </span><em>H. pylori</em><span> infected patients with UD in four European countries. Genomic DNA was isolated from 314 </span><em>H. pylori</em> strains: Germany (GER; <em>n</em> <!-->=<!--> <!-->92), Sweden (SWE, <em>n</em> <!-->=<!--> <!-->74), Portugal (POR, <em>n</em> <!-->=<!--> <!-->91) and Finland (FIN, <em>n</em> <!-->=<!--> <!-->57). The frequencies of <em>babA2</em> genotype varied from 35% to 60%. Triple-positive strains (<em>vacAs1</em>+, <em>cagA</em>+ and <em>babA2</em>+) were significantly associated with UD in GER and POR and were closely correlated with UD in FIN, but not in SWE. Classification as triple-positive strains had a higher specificity for detection of UD in GER, POR and FIN than <em>type1</em> or <em>cagA</em>+ strains. In vitro adhesion assays revealed that Swedish strains showed high adhesion properties and were thus correlated with the diagnosis of UD, although PCR detected the <em>babA2</em> gene at lower frequencies and failed to show a correlation with UD. This finding appears to reflect allelic variations of the <em>babA2</em> gene in SWE, although adhesive properties of the strains are retained.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 2","pages":"Pages 151-156"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2004.10.010","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25089015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A genetic locus of Helicobacter pylori inversely associated with gastric intestinal metaplasia","authors":"Quanjiang Dong ,&nbsp;Maria O’Sullivan ,&nbsp;Abdurrazag Nami ,&nbsp;Paul Dowling ,&nbsp;Gwen Murphy ,&nbsp;Martin Buckley ,&nbsp;Colm O’Morain","doi":"10.1016/j.femsim.2005.02.003","DOIUrl":"10.1016/j.femsim.2005.02.003","url":null,"abstract":"<div><p>The genomic contents of <span><span>Helicobacter pylori</span></span><span><span><span> strain C1 from a patient with gastric cancer and strain 98587 from a patient with duodenal ulcer disease were compared using a rapid </span>subtractive hybridisation approach. A total of 11 tester-specific sequences representing gene specificity, DNA rearrangement and sequence variation were identified. This included two novel sequences, clone </span>P32 and clone F5, which have no significant homologue in the database. </span><em>H. pylori</em><span> strains positive for clone P32 were less prevalent in patients with gastric intestinal metaplasia (12.5%) than in duodenal ulcer (39.1%) (</span><em>p</em>\u0000<!--> <!-->=<!--> <span>0.036), or chronic gastritis (38.1%) (</span><em>p</em>\u0000<!--> <!-->=<!--> <!-->0.036). The results suggest that <em>H. pylori</em> clone P32 is potentially a useful marker for distinguishing intestinal metaplasia associated strains from others.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 2","pages":"Pages 243-249"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2005.02.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25088332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helicobacter pylori disulphide reductases: role in metronidazole reduction","authors":"Nadeem O. Kaakoush,&nbsp;George L. Mendz","doi":"10.1016/j.femsim.2004.11.007","DOIUrl":"10.1016/j.femsim.2004.11.007","url":null,"abstract":"<div><p><span><span><span>Disulphide </span>reductases play an important role in maintaining intracellular </span>redox potential. Three disulphide reductase activities were identified in </span><span><em>Helicobacter pylori</em></span><span>, which used dithiobis-2-nitrobenzoic acid, glutathione or </span><span>l</span><span><span>-cystine and ferredoxin as substrates. The kinetic parameters of these activities were determined and it was demonstrated that the reductase activities were inhibited by the presence of </span>metronidazole. Substrate competition experiments served to show inhibition of metronidazole reduction by dithiobis-2-nitrobenzoic acid, glutathione and ferredoxin in lysates from metronidazole susceptible and resistant matched pairs of strains. The study demonstrated that the activities of three disulphide reductases were modulated by the presence of metronidazole, and that metronidazole reduction was inhibited by the presence of disulphide reductase substrates.</span></p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 2","pages":"Pages 137-142"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2004.11.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25089013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restriction of DNA encoding selectable markers decreases the transformation efficiency of Helicobacter pylori","authors":"Rebecca J. Gorrell ,&nbsp;Ji Yang ,&nbsp;Johannes G. Kusters ,&nbsp;Arnoud H.M. van Vliet ,&nbsp;Roy M. Robins-Browne","doi":"10.1016/j.femsim.2004.10.019","DOIUrl":"10.1016/j.femsim.2004.10.019","url":null,"abstract":"<div><p><span><span>Helicobacter pylori</span></span><span> populations recovered from the human stomach display extensive recombination and quasispecies development, and this suggests frequent exchange of DNA between different strains in vivo. In vitro, however, most </span><em>H. pylori</em> strains display restriction to the uptake of non-self DNA, as measured using selectable markers, regardless of their competency for transformation with self DNA. We have examined the effect of different selectable markers on double-crossover recombination efficiencies in three reference strains (1061, 26695 &amp; SS1) and one clinical isolate (CHP1) of <em>H. pylori</em>. All strains were efficiently transformable to kanamycin or chloramphenicol resistance by using self-genomic DNA from isogenic mutants bearing the <em>aphA3</em> or <em>cat</em> cassettes, respectively. However, strains 26695 and CHP1 showed a 3–5-log reduction in transformation efficiency by non-self recombinant DNA containing <em>aphA3</em>, when compared to <em>cat</em><span>. Strain 1061 readily accepted either cassette, and strain SS1 was poorly tolerant of any non-self DNA. Genome-wide random mutagenesis of these strains was only achievable with a selectable marker that allowed high transformation efficiency. Digestion of </span>³²P-labelled cassettes by <em>H. pylori</em> lysates mirrored the transformation results and indicated that in some strains these cassettes are the targets of enzymatic restriction.</p></div>","PeriodicalId":12220,"journal":{"name":"FEMS immunology and medical microbiology","volume":"44 2","pages":"Pages 213-219"},"PeriodicalIF":0.0,"publicationDate":"2005-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.femsim.2004.10.019","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25087292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}