SAR Journal of Medical Biochemistry最新文献

{"title":"Caffeine's Effect on Blood Pressure in Sudanese Women in East Gezira State-Sudan, 2018","authors":"Abderrhman Ahmed Mohamed Ismaeil, Nahla Ahmed Mohamed Abderahman, Mohammed Ahmed Ibrahim Ahmed, Nihal Abdalla, M. Hamad","doi":"10.36346/sarjmb.2022.v03i03.004","DOIUrl":"https://doi.org/10.36346/sarjmb.2022.v03i03.004","url":null,"abstract":"Background: Caffeine is a significant component of coffee, which is one of the most extensively, consumed non-alcoholic beverages. Caffeine is a methylxanthine-class central nervous system stimulant that is one of the most extensively utilized medications on the planet. Caffeine stimulates the central nervous system and is perhaps the most extensively used psychoactive stimulant. It causes gastrointestinal disturbances, tremor, headache, and sleeplessness, palpitations, cardiac arrhythmias, and it has been proposed that caffeine is possibly hypertensive. Aim: The goal of the study was to see how caffeine affected the blood pressure of Sudanese adult females in Gezira state's east during July and August 2018. Methods: A descriptive cross-sectional prospective study of 400 females aged 18 to 70 years old from various areas of Gezira State was conducted. Caffeine was not consumed by the participants for 12 hours before to the test. Blood pressure was monitored at baseline after 20 minutes of rest, then 45 minutes later after consuming a 150 mL cup of boiling coffee (120 mg caffeine) (each cup contain teaspoon 10 g of coffee powder). A questionnaire was created to collect personal and demographic information. Body mass index (BMI) was computed after measuring weight and height. Results: The researchers discovered that 120 mg of caffeine increased systolic blood pressure by 5 to 18 mmHg, diastolic blood pressure by 6 to 16 mmHg, and mean blood pressure by 3 to 13 mmHg. Caffeine had a higher effect in older and hypertensive people, according to the study, with a P value of <0.0001. With regular coffee consumption, there was no tolerance to the pressure impact of caffeine, according to the study. There was also racial variance in the caffeine presser response, with the Tama tribe having a stronger response and the Rufaah tribe having a higher baseline BP (P value is <0.0001). In addition, the prevalence of hypertension was 5.8% lower in rural areas. Conclusion: Caffeine raises systolic, diastolic, and mean blood pressure in Sudanese adult females, with a particularly noticeable effect on the elderly and hypertensive.","PeriodicalId":122039,"journal":{"name":"SAR Journal of Medical Biochemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131159007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human Associated Microbiota Identification by Using Sequence-Based Methods","authors":"M. Al-Bayati, F. N. Jaafar, H. Musafer, M. Azeez, Zahraa Kareem Raheem","doi":"10.36346/sarjmb.2022.v03i03.003","DOIUrl":"https://doi.org/10.36346/sarjmb.2022.v03i03.003","url":null,"abstract":"The microbiota can be defined as the community of microorganisms that live in a particular environment and as we all know, different types of microbes colonized the human body, derived from bacterial communities, microbial eukaryotes and viruses that are specific for each anatomical sites or environments. The next- generation DNA sequencing has allowed more and more advanced sampling and analysis of these complex systems by methods called culture- independent, these methods are indicating the differences in community structure between individuals, between diseased states and healthy and between anatomical sites. The advent of next generation sequencing (NGS) offered a cost-effective method that eliminated the cloning step by amplifying 16S rRNA genes using primers containing sequencing connecters and barcodes. However, this sequencing may not resolve closely related species at all times and probably miss the intra-species diversity. A shotgun sequencing was developed for direct sequencing of DNA. Metagenomics is the practice of sequencing DNA from the genomes of all organisms present in a particular sample, and has become a common method for the study of microbiota population structure and function. As sequencing-based microbiota analysis continues to be the greatest general technique across the arena, this review aim is to provide a general introduction to the technical opportunities and trials of sequence-based identification of human associated Microbiota and for understanding of the human Microbiota and their effect on human health and diseases.","PeriodicalId":122039,"journal":{"name":"SAR Journal of Medical Biochemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131734934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunological Detection of Interleukin-1alpha (IL-1α) in Iraqi Women with Polycystic Ovarian Syndrome","authors":"Z. A. Hlail, K. Mohammed","doi":"10.36346/sarjmb.2022.v03i03.002","DOIUrl":"https://doi.org/10.36346/sarjmb.2022.v03i03.002","url":null,"abstract":"Background: Interleukin-1 considered as a cytokine type multifunction and it is a feature that causes inflammation. Aim and Objective: to detect and study the immunological and molecular affect of Interleukin-1 α (IL-1 α) in Iraqi Women with Polycystic Ovarian Syndrome. Materials and Methods: 250 Iraqi women, their ages were from 15 to 50 years, they were divided into two groups: study group (n=125, Polycystic Ovary Syndrome PCOS) while the other group (n=125 control group mainly normal women), blood samples were taken in the second menstruate cycle day, the concentration of IL-1 α was calculated for each group, and after this determine the IL-1 α SNP (IL-1 α Rs1800787 A/G) by using real time PCR. Results: PCSO patients show a noticeable difference compared with other group. The mean level of IL-1α was 69.69± 93.38 (Pg /ml), 3.52E2± 139.15 (Pg /ml) in PCOS and healthy control respectively. The levels were ranging between 0 and 591 Pg/ml. The GG genotype (mutant gene) was detected in 94/125 (75.2%) of PCOS, and the AG genotype was detected in 31/125 (24.8%) of PCOS. The AA genotype (wild type) was not detected in both healthy and PCOS groups. Conclusions: The results showed high significant association of G allele in IL-1 α Rs1800787 A/G gene SNP (A/G) with PCOS, (P<0.0001), this increasing may related to ovulation lack, while the polymorphism in IL-1 α, can be a predispose aspect for the PCOS weakness.","PeriodicalId":122039,"journal":{"name":"SAR Journal of Medical Biochemistry","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130968139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Bacteria Species Associated with the Skin and Oral Surface of Different Occupational Groups","authors":"I. O, Airefetalor Ai, Omolumen Le, Osagiede Ek, Ikede Re, Ken-Iyevhobu, Ba, E. A","doi":"10.36346/sarjmb.2022.v03i03.001","DOIUrl":"https://doi.org/10.36346/sarjmb.2022.v03i03.001","url":null,"abstract":"The normal human skin is colonized by huge numbers of bacteria that live as commensals on its surface. At times bacteria not normally found there may colonize the epidermis and lead rapidly to disease. The oral cavity is comprised of many surfaces, each coated with a plethora of bacteria, the proverbial bacterial biofilm. Some of these bacteria have been implicated in oral diseases such as caries and periodontitis, which are among the most common bacterial infections in humans. In addition, specific oral bacterial species have been implicated in several systemic diseases, such as bacterial endocarditis, aspiration pneumonia, osteomyelitis in children, preterm low birth weight, and cardiovascular disease. The aim of this study is to evaluate the bacteria species associated with the skin and orals of different occupational groups in Ekpoma. This study was carried out in the Ekpoma, Esan West Local Government Area of Edo State. Participants were selected from the list of five (5) different occupational groups (Students, Office workers, Bike riders, Food handlers and Brick layers) in Ekpoma. Informed consent was sought from the various individuals. A total of one hundred (100) samples were collected from different occupational groups in the study area. The sample analysis was carried out using standard methods. Results obtained showed the rate of bacterial isolates from the skin and orals of the occupational groups studied is 63% and 43% respectively. Out of the 20 samples collected from the skin of each occupations, 9(45%) were positive for students, 16(80%) were for bike riders, 8(40%) for office workers, 17(85%) were for food handlers and 13(33%) for bricklayers. Food handlers appeared to be the occupation with the highest bacterial isolates on the skin followed by bike riders, bricklayers, students and office workers being the least. While out of the 20 samples collected from the mouth of each occupations, 7(35%) were positive for students, 11(55%) were for bike riders, 4(20%) for office workers, 12(60%) were for food handlers and 9(45%) for bricklayers. Office workers the highest bacteria isolates in the mouth followed by bike riders, food handlers and students been the least. In order to meet the huge challenge of occupational safety in the 21st century, a coordinative and cooperative approach is required. This will be a major task of the public health community and will require the use of new methods of identifying, monitoring and assessing of skin and oral infections, including the wide application of the hazard analysis and critical control point system.","PeriodicalId":122039,"journal":{"name":"SAR Journal of Medical Biochemistry","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127096546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Micro RNA the Important Biomarker in Cancer","authors":"Dr. Hiba Sabah Jasim","doi":"10.36346/sarjmb.2022.v03i02.002","DOIUrl":"https://doi.org/10.36346/sarjmb.2022.v03i02.002","url":null,"abstract":"MicroRNAs are a set of short noncoding RNAs that post transcriptionally control the gene expression through matching with its corresponding mRNAs. The down regulated of micro RNAs may be suggested as a novel kind of “oncomirs”or “tumor suppressors,” acting an important effect in the development of carcinoma. Employing genome wide detection techniques, common erratic expression types of micro RNAs have been recognized in a wide arrangement of cancers in human, demonstrate huge potential as modern detection and predictive agents of up normality and elevation of sensitivity and specificity. The diagnosable micro RNAs in blood and the further body fluids with rise constancy supply a profuse origin for micro RNA based agents in cancer cases. In spite of the verity that a growing number of effort micro RNA agents have been determinate, the transmission of micro RNAs based agents from board to bedside as yet important treatment and control many challenges. This study will demonstrate the recent comprehensive of micro RNAs as important agents in cancer of human.","PeriodicalId":122039,"journal":{"name":"SAR Journal of Medical Biochemistry","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130375142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Pre-mixed Extract of Adansonia digitata and Garlic on Streptozotocin (STZ) Induced Hyperglycemic Rat Model","authors":"A. A, N. S, Tanko N. D, I. M., B. B. Sadiq, J. U. Mari, Alhassan A. J","doi":"10.36346/sarjmb.2022.v03i02.001","DOIUrl":"https://doi.org/10.36346/sarjmb.2022.v03i02.001","url":null,"abstract":"The study investigated the effect of Adansonia digitata and garlic on Blood glucose level in streptozotocin induced diabetic rats. Twenty (20) albino rats were divided into four groups (GI, II, III, and IV) of five rats each and their fasting blood glucose was noted prior to inducement with diabetes using streptozotocin (STZ) at dose of 50mg/kg body weight. GI serves as diabetic control, receives no extract, while GII, GIII and GIV were respectively administered with 500mg/Kg Adansonia digitata extract, 400mg/Kg garlic extract and 500mg/Kg Adansonia – Garlic premix orally for 12 days. The serum blood glucose was monitored at interval of three days, after which the rats were allowed for nine days without extract with periodic record of their blood glucose at interval of three days. The fasting blood glucose in each group 24 hours after streptozotocin (STZ) injection was significantly higher (P<0.05) when compared Zero hours (before induction). The periodic Blood glucose levels were significantly lower (P<0.001) in the Group treated with 500mg/kg of Adansonia digitata, 400mg/kg of garlic and 500mg/kg of the premix extract compared to the corresponding period of GI. On withdrawal of the extracts, blood glucose of the diabetics rats increase. It may be concluded that both Adansonia digitata and garlic may possess hypoglycemic activity which could be due to their phytochemical and mineral contents.","PeriodicalId":122039,"journal":{"name":"SAR Journal of Medical Biochemistry","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133682359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Profile and Outcome and Risk Factors of Cardiovascular Involvement in Hospitalized Children with Kawasaki Disease","authors":"A. K. A. A. K. Al-Miraj, S. M. R. S. M. Rezwanuzzaman, Sakera Khatun Mony Sakera Khatun Mony, Muhammad Abduz Zaher Muhammad Abduz Zaher, Md. Rezaul Alam Md. Rezaul Alam, Mohammad Ata Ullah Mohammad Ata Ullah, Sharmin Akter Sharmin Akter, Mohammad Maruf Reza Mohammad Maruf Reza","doi":"10.36346/sarjmb.2022.v03i01.001","DOIUrl":"https://doi.org/10.36346/sarjmb.2022.v03i01.001","url":null,"abstract":"Kawasaki disease (KD), also known as Kawasaki syndrome, is an acute febrile illness of unknown cause that primarily affects children younger than 5 years of age. The present study was conducted to study the clinical profile and outcome and risk factors of Kawasaki disease in children. This observational prospective study was conducted in cardiology ward with a diagnosis of Kawasaki disease from 1st June 2020 to 31 July 2021 in Bangabandhu Sheikh Mujib Medical University Hospital Dhaka, Bangladesh. Forty five (45) children aged between 3 months to 10 years were studied. Kawasaki disease is more prevalent among male child (73.33%). Most commonly encountered risk factor for cardiovascular involvement especially coronary dilatation or aneurysmal change is more observed in younger age of presentation. Fever, rash, conjunctivitis, erythema and edema of limbs, periungual skin desquamations are the presenting features. Cardiovascular involvement of left main coronary artery dilation is the most frequently observed aneurismal change observed among study population which was evident in 68% of the children having coronary involvement. All the study patient received high dose aspirin (100%) and 93% received IVIG of whom 6.66% had IVIG- resistant Kawasaki disease. Commonly occurring complication is uveitis (17.77%). Among the total 45 study population 1 patient expired (2.22%).Clinicians should have a high index of suspicion of Kawasaki disease in persistently febrile patients and once clinically diagnosed, echocardiography should be done and IVIG therapy along with aspirin should be started, specially in infants (<6 months) as they have the higher tendency to develop coronary aneurysm.","PeriodicalId":122039,"journal":{"name":"SAR Journal of Medical Biochemistry","volume":"213 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121636245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}