Federation proceedings最新文献

Beriberi.

Federation proceedings Pub Date : 2005-07-01 DOI: 10.1001/jama.1914.02560310060034

R. Burgess, B. S. Platt, R. H. Follis, P. Handler, D. Denny-Brown, W. R. Aykroyd

引用次数: 0

Conjugation—Deconjugation Reactions in Drug Metabolism and Toxicity 药物代谢和毒性中的偶联-去偶联反应

Federation proceedings Pub Date : 1994-01-01 DOI: 10.1007/978-3-642-78429-3

F. Kauffman

引用次数: 145

Opioid regulation of food intake and body weight in humans. 阿片类药物对人类食物摄入和体重的调节。

Federation proceedings Pub Date : 1987-12-01 DOI: 10.1097/00132586-198712000-00028

R. Atkinson

引用次数: 33

Polyclonal and monoclonal antibodies as probes of rat hepatic cytochrome P-450 isozymes. 多克隆和单克隆抗体作为大鼠肝细胞色素P-450同工酶的探针。

Federation proceedings Pub Date : 1987-06-01

P E Thomas, S Bandiera, L M Reik, S L Maines, D E Ryan, W Levin

{"title":"Polyclonal and monoclonal antibodies as probes of rat hepatic cytochrome P-450 isozymes.","authors":"P E Thomas, S Bandiera, L M Reik, S L Maines, D E Ryan, W Levin","doi":"","DOIUrl":"","url":null,"abstract":"Cytochrome P-450 is the terminal oxidase of an electron transport system that is responsible for the oxidative metabolism of a large variety of endogenous and exogenous compounds. This broad substrate selectivity is caused by multiple isozymes of cytochrome P-450 and the wide substrate selectivity of many of these isozymes. We have isolated 11 isozymes of cytochrome P-450 from the livers of rats (cytochromes P-450a-P-450k). We have found both polyclonal and monoclonal antibodies increasingly useful to distinguish among these isozymes and to quantitate enzyme levels in liver microsomal preparations where as many as 15 or more cytochrome P-450 isozymes are present. Several of these isozymes show considerable immunochemical relatedness to each other, and operationally they can be grouped into families of immunochemically related isozymes that include cytochromes P-450b and P-450e in one family, cytochromes P-450c and P-450d in another, and cytochromes P-450f-P-450i, and P-450k in a third family. Immunoquantitation of some of these isozymes has revealed dramatic increases of over 50-fold in the levels of certain of these isozymes when exogenous compounds are administered to rats.","PeriodicalId":12183,"journal":{"name":"Federation proceedings","volume":"46 8","pages":"2563-6"},"PeriodicalIF":0.0,"publicationDate":"1987-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14424730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Needed in science--blacks and Hispanics. 科学需要——黑人和西班牙裔。

Federation proceedings Pub Date : 1987-06-01

B H Pickett

引用次数: 0

Cardioselective muscarinic antagonists. 心脏选择性毒蕈碱拮抗剂。

Federation proceedings Pub Date : 1987-06-01

引用次数: 0

The immunochemistry of solid-phase sandwich enzyme-linked immunosorbent assays. 固相三明治酶联免疫吸附试验的免疫化学。

Federation proceedings Pub Date : 1987-06-01

J E Butler, J H Peterman, M Suter, S E Dierks

{"title":"The immunochemistry of solid-phase sandwich enzyme-linked immunosorbent assays.","authors":"J E Butler, J H Peterman, M Suter, S E Dierks","doi":"","DOIUrl":"","url":null,"abstract":"Multivalent antigens (Ags) such as membrane proteins can be quantitated by using sandwich enzyme-linked immunosorbent assays (ELISAs), which typically show sensitivity from 0.1 to 50 ng/ml. The percentage of antigen that binds in the log-log linear region reflects the affinity of the capture antibody (CAb), and the range of linearity for assays conducted with a particular CAb is proportional to the antibody (Ab) concentration. The sandwich ELISA titration plot reflects the actual amount of Ag bound when asymmetrical configurations are used; steric hindrance that occurs with certain symmetrical configurations, especially when enzyme-Ab conjugates of greater than or equal to 10(6) daltons are used, can alter this relationship. Monoclonal CAbs bind less Ag than polyclonal CAbs. Immobilization of monoclonal CAbs by using a modified avidin-biotin system can result in greater antigen capture capacity (AgCC) than when the Abs are directly adsorbed on plastic. Adsorption of proteins on polystyrene is noncovalent and proportional to the amount added for up to 150 ng/200 microliter in a microtiter well. Adsorption can result in substantial loss of antigenic or antibody activity. Desorption is continuous at a low level and can negatively influence the results of an immunoassay. Data from microtiter sandwich ELISAs can be readily acquired and analyzed by using a computer-based analysis system (ELISANALYSIS) written for the IBM PC. This analytical system considers the immunochemical principles of sandwich ELISAs predicted theoretically and demonstrated empirically.","PeriodicalId":12183,"journal":{"name":"Federation proceedings","volume":"46 8","pages":"2548-56"},"PeriodicalIF":0.0,"publicationDate":"1987-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14718314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive immunological techniques for quantitating membrane antigens. 膜抗原定量的高灵敏度免疫技术。

Federation proceedings Pub Date : 1987-06-01

T K Shires

引用次数: 0

Strides in the technology of systems physiology and the art of testing complex hypotheses. 在系统生理学技术和测试复杂假设的艺术方面取得了长足的进步。

Federation proceedings Pub Date : 1987-06-01

J B Bassingthwaighte

{"title":"Strides in the technology of systems physiology and the art of testing complex hypotheses.","authors":"J B Bassingthwaighte","doi":"","DOIUrl":"","url":null,"abstract":"Scientific understanding in physics or physiology is based on models or theories devised to describe what is known, within the limits imposed by observation error. Carefully integrated models can be used for prediction, and the inferences assessed via further experiments designed to test the adequacy of the theory summarizing the state of knowledge. This is the systems approach, the basis of theoretical physiology; the models, like those of theoretical physics, should be firmly based on fundamental reproducible observations of a physical or chemical nature, held together with the principles of mathematics, logic, and the conservation of mass and energy. Modern computing power is such that comprehensive models can now be constructed and tested. For this approach data sets should include as many simultaneously obtained items of information of differing sorts as possible to reduce the degrees of freedom in fitting models to data. By taking advantage of large memories and rapid computation, modular construction techniques permit the formulation of multimodels covering more than a single hierarchical level, and thereby allow the investigator to understand the effects of controllers at the molecular level on overall cell or organism behavior. How does this influence the research and teaching practices of physiology? Because the computer also allows a new type of collaboration involving the networking of ideas, data bases, analytical techniques, and experiment designing, investigators in geographically distributed individual laboratories can plan, work, and analyze in concert. The prediction from this socioscientific model is therefore that networked computer-based modeling will serve to coalesce the ideas and observations of enlarging groups of investigators.","PeriodicalId":12183,"journal":{"name":"Federation proceedings","volume":"46 8","pages":"2473-6"},"PeriodicalIF":0.0,"publicationDate":"1987-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4132062/pdf/nihms203996.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14425605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization of the functional sites on the alpha chain of acetylcholine receptor. 乙酰胆碱受体α链功能位点的定位。

Federation proceedings Pub Date : 1987-06-01

M Z Atassi, B Mulac-Jericevic, T Yokoi, T Manshouri

{"title":"Localization of the functional sites on the alpha chain of acetylcholine receptor.","authors":"M Z Atassi, B Mulac-Jericevic, T Yokoi, T Manshouri","doi":"","DOIUrl":"","url":null,"abstract":"A comprehensive synthetic approach, previously developed in this laboratory, has been applied to systematically screen the entire extracellular part (residues 1-210) of the alpha chain of the Torpedo californica acetylcholine receptor (AChR) for the profiles of the continuous regions that are recognized by antibodies against free, or membrane-sequestered, AChR; the regions recognized by AChR-primed T cells; the regions that bind alpha-bungarotoxin and cobratoxin; and an acetylcholine-binding region. Eight continuous antigenic sites were localized in this part of the alpha chain by all of the antisera tested. The sites were independent of the host species from which the antisera were obtained and were also similar to antisera against the isolated pentameric AChR or against the membrane-sequestered AChR. Six regions were found to stimulate AChR-primed T cells (T sites). Three of the T sites coincided with regions recognized by antibodies. At least two T sites had no detectable antibody responses directed to them. Five toxin-binding regions were localized, and may constitute distinct sites or, alternatively, different faces in one (or more) sites. Some of these regions coincided with regions recognized by anti-AChR antibodies. One of the toxin-binding regions bound acetylcholine, and immunization with this peptide induced experimental autoimmune myasthenia gravis.","PeriodicalId":12183,"journal":{"name":"Federation proceedings","volume":"46 8","pages":"2538-47"},"PeriodicalIF":0.0,"publicationDate":"1987-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13585879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0