Experimental and molecular pathology最新文献_第10页

Retraction notice to “Lycium barbarum polysaccharides alleviate hydrogen peroxide-induced injury by up-regulation of miR-4295 in human trabecular meshwork cells” [Experimental and Molecular Pathology 106 (2019) 109–115] 关于“枸杞多糖通过上调人小梁网细胞miR-4295减轻过氧化氢损伤”的撤回通知[实验与分子病理学106 (2019)109-115]

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-08-01 DOI: 10.1016/j.yexmp.2022.104808

Yuxia Liu , Yan Zhang

引用次数: 0

Phloretin enhances autophagy by impairing AKT activation and inducing JNK-Beclin-1 pathway activation 根皮素通过抑制AKT激活和诱导JNK-Beclin-1通路激活来增强自噬

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-08-01 DOI: 10.1016/j.yexmp.2022.104814

Chenghe Fan , Yilin Zhang , Yu Tian, Xinyu Zhao, Junfang Teng

{"title":"Phloretin enhances autophagy by impairing AKT activation and inducing JNK-Beclin-1 pathway activation","authors":"Chenghe Fan , Yilin Zhang , Yu Tian, Xinyu Zhao, Junfang Teng","doi":"10.1016/j.yexmp.2022.104814","DOIUrl":"10.1016/j.yexmp.2022.104814","url":null,"abstract":"<div><p>Phloretin is a type of dihydrochalcone that is primarily found in apples and has been reported to possess various potent biological activities, such as anticancer, antioxidant and anti-inflammatory effects. Our previous study has shown that phloretin induces apoptosis in human glioblastoma. In this study, we found that phloretin induced autophagy in SH-SY5Y cells by decreasing p-AKT and p-mTOR levels in the AKT/mTOR pathway and increasing the activation of JNK, the phosphorylation of c-Jun and the expression of Beclin-1. Moreover, the upregulation of Beclin-1 was decreased by SP600125 or a siRNA against c-Jun. Furthermore, SP600125 and siRNAs against c-Jun and Beclin-1 inhibited phloretin-induced autophagy. In addition, inhibition of phloretin-induced autophagy by cotreatment with phloretin and 3-MA decreased phloretin-induced cytotoxicity to SH-SY5Y cells. In conclusion, our results suggest that the AKT/mTOR pathway and JNK-mediated Beclin-1 expression are involved in phloretin-induced autophagy. Phloretin can be used to protect neurons during phloretin treatment of glioblastoma.</p></div>","PeriodicalId":12176,"journal":{"name":"Experimental and molecular pathology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0014480022000776/pdfft?md5=2398f16c35670811fbd96f2e8b30e5df&pid=1-s2.0-S0014480022000776-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40647858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sirt2 positively regulates muscle regeneration after Notexin-induced muscle injury Sirt2正调控诺特辛诱导的肌肉损伤后的肌肉再生

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-08-01 DOI: 10.1016/j.yexmp.2022.104798

Eun-Joo Lee , Myeong-Mi Lee , SunYoung Park, Kyu-Shik Jeong

{"title":"Sirt2 positively regulates muscle regeneration after Notexin-induced muscle injury","authors":"Eun-Joo Lee , Myeong-Mi Lee , SunYoung Park, Kyu-Shik Jeong","doi":"10.1016/j.yexmp.2022.104798","DOIUrl":"https://doi.org/10.1016/j.yexmp.2022.104798","url":null,"abstract":"<div><p><span><span>Sirt2 regulates various biological processes by deacetylating target genes. Despite roles in regulating proliferation, cell cycle, and </span>glucose metabolism, which are closely associated with </span>skeletal muscle<span><span> physiology, Sirt2 functions in this tissue remain unclear. In this study, genetic deletion<span><span> of Sirt2 delayed muscle regeneration after Notexin-induced muscle injury. Gene expressions of </span>myogenic regulatory factors, including Myf5, </span></span>MyoD<span><span>, and Myogenin, and cell cycle regulators, such as </span>cyclin D1<span><span><span><span> and CDK2, were repressed in Sirt2 knockout mice after injury. Also, Sirt2 knockout mice presented </span>muscle atrophy after muscle injury which is associated with the down-regulation of anabolic signaling and the up-regulation of catabolic signaling, in particular, increased </span>atrogin1<span> transcriptional expression. Thus, Sirt2 positively regulated skeletal muscle regeneration after muscle injury by regulating transcriptional expression involved in myogenesis, cell cycle, and anabolic and catabolic signaling. Based on the in vivo analyses, Sirt2 could function as an interventional therapeutic for chronic </span></span>myopathy, which is characterized by impaired muscle regeneration and muscle atrophy.</span></span></span></p></div>","PeriodicalId":12176,"journal":{"name":"Experimental and molecular pathology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71787919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

The development of an indel panel for microchimerism detection 微嵌合检测用indel面板的研制

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-08-01 DOI: 10.1016/j.yexmp.2022.104804

Sofie D.H. Olsen , Astrid M. Kolte , Nina Bang , Maria Christine Krog , Rudi Steffensen , Henriette S. Nielsen , Marianne A. Jakobsen

{"title":"The development of an indel panel for microchimerism detection","authors":"Sofie D.H. Olsen , Astrid M. Kolte , Nina Bang , Maria Christine Krog , Rudi Steffensen , Henriette S. Nielsen , Marianne A. Jakobsen","doi":"10.1016/j.yexmp.2022.104804","DOIUrl":"10.1016/j.yexmp.2022.104804","url":null,"abstract":"<div><h3>Objectives</h3><p>The aim of the study was to create a simple assay for microchimerism detection independent of sex and without HLA genotyping.</p></div><div><h3>Methods</h3><p><span><span>The method is based on detection of insertion or deletions utilizing a multiplex PCR followed by fragment analysis by </span>capillary electrophoresis, and probe-based qPCR assays. A total of 192 samples, taken either before pregnancy, during 1st trimester, or either during </span>2nd trimester<span><span> or at miscarriage, obtained from a cohort of 97 female patients with either primary or secondary recurrent pregnancy loss, were screened for fetal microchimerism by the </span>indel panel as well as an existing assay based on detection of the Y-chromosome marker; DYS14.</span></p></div><div><h3>Results</h3><p><span>The overall prevalence of DYS14 positive samples was 29% (55/192) whereas 32% (61/192) tested positive by the indel method. There was an overall agreement of 64% (122/192) between the results obtained by the two methods. A Fisher's Exact test showed no statistic significant difference in the prevalence of microchimerism detected by the two methods at any of the three times of sampling. The distribution of the number of positive wells detected by both methods were compared by a Mann-Whitney </span><em>U</em> test, which showed no statistically significant difference at any of the three times of sampling.</p></div><div><h3>Conclusion</h3><p>The data indicates that microchimerism can be detected efficiently by the indel method. This makes it possible to detect both female and male cells without the need of HLA-genotyping. Furthermore, the indel method has potential to be implemented as a routine analysis. This will remove the sex bias in future explorations of the role microchimerism plays in health and disease.</p></div>","PeriodicalId":12176,"journal":{"name":"Experimental and molecular pathology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39991573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction notice to “Ligustrazine promoted hypoxia-treated cell growth by upregulation of miR-135b in human umbilical vein endothelial cells” [Experimental and Molecular Pathology 106 (2019) 102–108] 关于“川芎嗪通过上调人脐静脉内皮细胞miR-135b促进缺氧处理细胞生长”的撤稿通知[实验与分子病理学106 (2019)102-108]

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-08-01 DOI: 10.1016/j.yexmp.2022.104786

Shujing Wei , Hui Wang

引用次数: 0

Overexpression of SHARPIN promotes tumor progression in ovarian cancer. SHARPIN 的过表达会促进卵巢癌的肿瘤进展。

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-07-04 DOI: 10.1016/j.yexmp.2022.104806

Guanghui Wang, Zi Zhuang, Jianxiang Cheng, Fan Yang, Dachun Zhu, Zhiyuan Jiang, Wensheng Du, Siyuan Shen, Ju Huang, Lei Hua, Youguo Chen

{"title":"Overexpression of SHARPIN promotes tumor progression in ovarian cancer.","authors":"Guanghui Wang, Zi Zhuang, Jianxiang Cheng, Fan Yang, Dachun Zhu, Zhiyuan Jiang, Wensheng Du, Siyuan Shen, Ju Huang, Lei Hua, Youguo Chen","doi":"10.1016/j.yexmp.2022.104806","DOIUrl":"10.1016/j.yexmp.2022.104806","url":null,"abstract":"<p><p>SHARPIN (Shank-associated RH domain interacting protein) plays an important role in tumorigenesis. However, its role in ovarian cancer remains largely unknown. To investigate this issue, we systematically analyzed the amplification and expression of the SHARPIN in the TCGA database. From the database, we found that SHARPIN was amplified in ovarian cancer compared to normal ovarian tissue, and the mRNA level of SHARPIN was significantly elevated in ovarian cancer compared to non-tumorigenic ovarian tissue. In addition, we observed similar results from ovarian cancer cell lines and clinical samples from ovarian cancer patients, which indicated that increased SHARPIN expression is associated with tumorigenesis in ovarian cancer. SHARPIN knockdown inhibited the migration and invasion of ovarian cancer cells, also inhibited cell cycle and promoted apoptosis, thereby suppressing cell proliferation. RNA-seq results showed that SHARPIN significantly increased the expression of P53 and P21 and decreased the expression of Cyclin D1 and c-Myc, all of which are involved in the regulation of cell proliferation. Subsequent mechanistic exploration revealed that SHARPIN knockdown increased the expression of caspases 3 and 9, leading to apoptosis of ovarian cancer cells. We also found that high expression of SHARPIN was associated with poor prognosis of ovarian cancer patients. Collectively, we demonstrated a positive correlation between SHARPIN and ovarian cancer progression and provide a basis for combined targeted therapy strategies for future ovarian cancer treatment.</p>","PeriodicalId":12176,"journal":{"name":"Experimental and molecular pathology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2022-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40479028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression level of miR-155, miR-15a and miR-19a in peripheral blood of ductal carcinoma breast cancer patients: Possible bioindicators for cellular inherent radiosensitivity 导管癌乳腺癌患者外周血中miR-155、miR-15a和miR-19a的表达水平:细胞固有放射敏感性的可能生物指标

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-06-01 DOI: 10.1016/j.yexmp.2022.104758

Fatemeh Rajabi, Hossein Mozdarani

{"title":"Expression level of miR-155, miR-15a and miR-19a in peripheral blood of ductal carcinoma breast cancer patients: Possible bioindicators for cellular inherent radiosensitivity","authors":"Fatemeh Rajabi, Hossein Mozdarani","doi":"10.1016/j.yexmp.2022.104758","DOIUrl":"10.1016/j.yexmp.2022.104758","url":null,"abstract":"<div><p>Examination of cellular radiosensitivity (RS) helps prevent the adverse side-effects of radiotherapy in radioresistant tumors. We aim to study whether miRNA-155 (miR-155), miR-19a and miR-15a can predict inherent RS according to cellular RS in breast cancer (BC) patients.</p><p>This study was done on the blood samples of 40 invasive ductal carcinoma (IDC) BC patients and 15 healthy women. G2 assay was performed to evaluate cellular RS. To study the expression level of these miRNAs in blood, qRT-PCR was used. The sensitivity and specificity of the studied miRNAs were assessed by the receiver operating characteristic (ROC) curve.</p><p><span>The yield of spontaneous (SY) and radiation-induced (RIY) chromatid breaks (CBs) was significantly different between control and patient groups (</span><em>p</em> < 0.0001). A cut-off value was specified to recognize the patients with cellular RS from those without. Expression of miR-15a was significantly downregulated (<em>p</em> < 0.0001) in BC patients. However, miR-19a showed upregulation in the blood of BC patients. It was also found the expression level of miR-155 and miR-19a were significantly associated with frequency of CBs (FCB) (<em>p</em> < 0.05). ROC curve analysis manifested that the miR-15a and miR-19a differentiate BC patients and healthy women with 0.91 and 0.68 yielding an area under the ROC curve, respectively. miR-155 and miR-19a discriminate between BC patients with and without cellular RS with area under the ROC curve 0.98 and 0.68.</p><p>Our findings uncovered miR-155 and miR-19a could be applied as a bioindicator to predict cellular radiosensitivity of BC patients.</p></div>","PeriodicalId":12176,"journal":{"name":"Experimental and molecular pathology","volume":null,"pages":null},"PeriodicalIF":3.6,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40331218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Retraction notice to “LncRNA MALAT1 knockdown alleviates oxygen-glucose deprivation and reperfusion induced cardiomyocyte apoptotic death by regulating miR-122” [Experimental and Molecular Pathology 111 (2019) 104325] 关于“LncRNA MALAT1敲低通过调节miR-122减轻氧糖剥夺和再灌注诱导的心肌细胞凋亡死亡”的撤回通知[实验与分子病理学111 (2019)104325]

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-06-01 DOI: 10.1016/j.yexmp.2022.104778

Licheng Gong, Hong Chang, Haiming Xu

引用次数: 0

Retraction notice to "The role of cardiac fibroblasts in post-myocardial heart tissue repair" [Experimental and Molecular Pathology 101/2 (2016) 231-240]. 关于“心肌成纤维细胞在心肌后心脏组织修复中的作用”的撤回通知[实验与分子病理学](2016)231-240。

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-06-01 DOI: 10.1016/j.yexmp.2022.104783

D. Chistiakov, A. N. Orekhov, Y. Bobryshev

引用次数: 0

The temporal impact of erythropoietin administration on mitochondrial function and dynamics in cardiac ischemia/reperfusion injury. 促红细胞生成素对心肌缺血/再灌注损伤线粒体功能和动力学的时间影响。

IF 3.6 4区医学

Experimental and molecular pathology Pub Date : 2022-06-01 DOI: 10.1016/j.yexmp.2022.104802

Juthipong Benjanuwattra, N. Apaijai, T. Chunchai, K. Singhanat, B. Arunsak, K. Intachai, S. Chattipakorn, N. Chattipakorn

引用次数: 2