利用下一代DNA测序分析前列腺腺癌的微生物群落特征和赭曲霉毒素A产生真菌的证据

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Stephen E. Fry , Mitchell Kaye , Dara S. Missan , Christian Becker , Matthew Shabilla , Delyn Martinez , Erin Bossert , Jeremy Ellis
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引用次数: 0

摘要

背景:前列腺癌是发达国家的主要癌症和主要死亡原因。病因多样,有潜在的患者遗传、环境因素和微生物关联。微生物的DNA测序可以检测潜在的疾病关系。目的对肿瘤微生物区系进行16S(原核)和18S(真核)rDNA靶向测序。12例经活检证实为前列腺腺癌的患者接受择期腹腔镜前列腺切除术。采用PCR和扩增子测序;部分测序结果经特殊染色证实。由泌尿科医生招募的患者在门诊接受“达芬奇”机器人辅助手术进行根治性前列腺切除术。在前列腺切除术时,样本在医院外科套房中进行分配。参与者:小患者被要求以先到为基础进入研究。结果测量与统计分析:12例患者平均年龄64.3岁。结果与限制检测了dna读数,并通过“最佳匹配”鉴定出属于Perkinsus, Hydrurus, diverspora和funeliformis属,少数样品显示细菌。在12例患者中,11例患者检测到与肾小球菌类丛枝菌根真菌相匹配的DNA序列;苔藓漏斗孢和异形孢。丛枝菌根真菌的特异性PCR不能确定肾小球菌属;深入的分类学分析表明,这是一种较新的真菌类群,不属于公认的真菌门。组织切片钙粉白染色证实了所有12例病例中潜在的真菌标志物。12例患者标本均经免疫荧光法鉴定出赭曲霉毒素A抗原。该研究受到样本量少和无疾病正常对照的限制。结论真菌可能在前列腺腺癌的发生中起重要作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbial community profiling by next-generation DNA sequencing of adenocarcinoma of the prostate with evidence of ochratoxin A producing fungi

Background

Prostatic carcinomas are a leading cancer and leading cause of mortality in the developed world. The etiology is diverse with underlying patient genetics, environmental factors, and microbial associations. Sequencing DNA for microbes allows the detection of potential disease relationships.

Objective

Targeted 16S (prokaryotic) and 18S (eukaryotic) rDNA sequencing was performed to map the tumor microbial flora.

Design

Twelve patients undergoing elective laparoscopic prostatectomy for biopsy proven adenocarcinoma of the prostate were enrolled. PCR and amplicon based sequencing was conducted; a portion of the sequencing results were confirmed by special stains.

Setting

Patients were recruited by the urologist were prospectively scheduled for radical prostatectomy by ‘Da Vinci’ robotically assisted procedure in an outpatient setting. Samples were portioned in the hospital surgical suite at the time of prostatectomy.

Participants

Male patients were requested to enter the study on a first come basis. Outcome Measurement and Statistical Analysis: Average age of the 12 participants was 64.3 years.

Results and limitations

DNA reads were detected and by ‘best match’ were identified belonging to Perkinsus, Hydrurus, Diversispora and Funneliformis genera, few samples displayed bacteria. Out of the 12 total patients, 11 patients had detectable DNA sequences matching arbuscular mycorrhizal fungi in the Glomeromycetes Class; Funneliformis mosseae and Diversasporum versiformis. Specific PCR for arbuscular mycorrhizal fungi failed to confirm Glomeromycetes Class; in-depth taxonomic analysis suggests a newer fungal grouping, not falling within an accepted Phylum of fungi. Calcoflour white staining of histological sections confirmed potential fungal markers in all 12 cases. Ochratoxin A antigen was identified by immunofluorescence in all 12 patient samples. The study was limited by the low sample volume and disease free normal controls.

Conclusions

Fungi may play a significant role in adenocarcinoma of the prostate.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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