Stephen E. Fry , Mitchell Kaye , Dara S. Missan , Christian Becker , Matthew Shabilla , Delyn Martinez , Erin Bossert , Jeremy Ellis
{"title":"利用下一代DNA测序分析前列腺腺癌的微生物群落特征和赭曲霉毒素A产生真菌的证据","authors":"Stephen E. Fry , Mitchell Kaye , Dara S. Missan , Christian Becker , Matthew Shabilla , Delyn Martinez , Erin Bossert , Jeremy Ellis","doi":"10.1016/j.yexmp.2022.104831","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Prostatic carcinomas are a leading cancer and leading cause of mortality in the developed world. The etiology is diverse with underlying patient genetics, environmental factors, and microbial associations. Sequencing DNA for microbes allows the detection of potential disease relationships.</p></div><div><h3>Objective</h3><p>Targeted 16S (prokaryotic) and 18S (eukaryotic) rDNA sequencing was performed to map the tumor microbial flora.</p></div><div><h3>Design</h3><p>Twelve patients undergoing elective laparoscopic prostatectomy for biopsy proven adenocarcinoma of the prostate were enrolled. PCR and amplicon based sequencing was conducted; a portion of the sequencing results were confirmed by special stains.</p></div><div><h3>Setting</h3><p>Patients were recruited by the urologist were prospectively scheduled for radical prostatectomy by ‘Da Vinci’ robotically assisted procedure in an outpatient setting. Samples were portioned in the hospital surgical suite at the time of prostatectomy.</p></div><div><h3>Participants</h3><p>Male patients were requested to enter the study on a first come basis. Outcome Measurement and Statistical Analysis: Average age of the 12 participants was 64.3 years.</p></div><div><h3>Results and limitations</h3><p>DNA reads were detected and by ‘best match’ were identified belonging to Perkinsus, Hydrurus, Diversispora and Funneliformis genera, few samples displayed bacteria. Out of the 12 total patients, 11 patients had detectable DNA sequences matching arbuscular mycorrhizal fungi in the Glomeromycetes Class; Funneliformis mosseae and Diversasporum versiformis. Specific PCR for arbuscular mycorrhizal fungi failed to confirm Glomeromycetes Class; in-depth taxonomic analysis suggests a newer fungal grouping, not falling within an accepted Phylum of fungi. Calcoflour white staining of histological sections confirmed potential fungal markers in all 12 cases. Ochratoxin A antigen was identified by immunofluorescence in all 12 patient samples. The study was limited by the low sample volume and disease free normal controls.</p></div><div><h3>Conclusions</h3><p>Fungi may play a significant role in adenocarcinoma of the prostate.</p></div>","PeriodicalId":12176,"journal":{"name":"Experimental and molecular pathology","volume":"128 ","pages":"Article 104831"},"PeriodicalIF":2.8000,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0014480022000946/pdfft?md5=e7aa9fd3770a6f4078827dd710806fca&pid=1-s2.0-S0014480022000946-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Microbial community profiling by next-generation DNA sequencing of adenocarcinoma of the prostate with evidence of ochratoxin A producing fungi\",\"authors\":\"Stephen E. Fry , Mitchell Kaye , Dara S. Missan , Christian Becker , Matthew Shabilla , Delyn Martinez , Erin Bossert , Jeremy Ellis\",\"doi\":\"10.1016/j.yexmp.2022.104831\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>Prostatic carcinomas are a leading cancer and leading cause of mortality in the developed world. The etiology is diverse with underlying patient genetics, environmental factors, and microbial associations. Sequencing DNA for microbes allows the detection of potential disease relationships.</p></div><div><h3>Objective</h3><p>Targeted 16S (prokaryotic) and 18S (eukaryotic) rDNA sequencing was performed to map the tumor microbial flora.</p></div><div><h3>Design</h3><p>Twelve patients undergoing elective laparoscopic prostatectomy for biopsy proven adenocarcinoma of the prostate were enrolled. PCR and amplicon based sequencing was conducted; a portion of the sequencing results were confirmed by special stains.</p></div><div><h3>Setting</h3><p>Patients were recruited by the urologist were prospectively scheduled for radical prostatectomy by ‘Da Vinci’ robotically assisted procedure in an outpatient setting. Samples were portioned in the hospital surgical suite at the time of prostatectomy.</p></div><div><h3>Participants</h3><p>Male patients were requested to enter the study on a first come basis. Outcome Measurement and Statistical Analysis: Average age of the 12 participants was 64.3 years.</p></div><div><h3>Results and limitations</h3><p>DNA reads were detected and by ‘best match’ were identified belonging to Perkinsus, Hydrurus, Diversispora and Funneliformis genera, few samples displayed bacteria. Out of the 12 total patients, 11 patients had detectable DNA sequences matching arbuscular mycorrhizal fungi in the Glomeromycetes Class; Funneliformis mosseae and Diversasporum versiformis. Specific PCR for arbuscular mycorrhizal fungi failed to confirm Glomeromycetes Class; in-depth taxonomic analysis suggests a newer fungal grouping, not falling within an accepted Phylum of fungi. Calcoflour white staining of histological sections confirmed potential fungal markers in all 12 cases. Ochratoxin A antigen was identified by immunofluorescence in all 12 patient samples. 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Microbial community profiling by next-generation DNA sequencing of adenocarcinoma of the prostate with evidence of ochratoxin A producing fungi
Background
Prostatic carcinomas are a leading cancer and leading cause of mortality in the developed world. The etiology is diverse with underlying patient genetics, environmental factors, and microbial associations. Sequencing DNA for microbes allows the detection of potential disease relationships.
Objective
Targeted 16S (prokaryotic) and 18S (eukaryotic) rDNA sequencing was performed to map the tumor microbial flora.
Design
Twelve patients undergoing elective laparoscopic prostatectomy for biopsy proven adenocarcinoma of the prostate were enrolled. PCR and amplicon based sequencing was conducted; a portion of the sequencing results were confirmed by special stains.
Setting
Patients were recruited by the urologist were prospectively scheduled for radical prostatectomy by ‘Da Vinci’ robotically assisted procedure in an outpatient setting. Samples were portioned in the hospital surgical suite at the time of prostatectomy.
Participants
Male patients were requested to enter the study on a first come basis. Outcome Measurement and Statistical Analysis: Average age of the 12 participants was 64.3 years.
Results and limitations
DNA reads were detected and by ‘best match’ were identified belonging to Perkinsus, Hydrurus, Diversispora and Funneliformis genera, few samples displayed bacteria. Out of the 12 total patients, 11 patients had detectable DNA sequences matching arbuscular mycorrhizal fungi in the Glomeromycetes Class; Funneliformis mosseae and Diversasporum versiformis. Specific PCR for arbuscular mycorrhizal fungi failed to confirm Glomeromycetes Class; in-depth taxonomic analysis suggests a newer fungal grouping, not falling within an accepted Phylum of fungi. Calcoflour white staining of histological sections confirmed potential fungal markers in all 12 cases. Ochratoxin A antigen was identified by immunofluorescence in all 12 patient samples. The study was limited by the low sample volume and disease free normal controls.
Conclusions
Fungi may play a significant role in adenocarcinoma of the prostate.
期刊介绍：
Under new editorial leadership, Experimental and Molecular Pathology presents original articles on disease processes in relation to structural and biochemical alterations in mammalian tissues and fluids and on the application of newer techniques of molecular biology to problems of pathology in humans and other animals. The journal also publishes selected interpretive synthesis reviews by bench level investigators working at the "cutting edge" of contemporary research in pathology. In addition, special thematic issues present original research reports that unravel some of Nature''s most jealously guarded secrets on the pathologic basis of disease.
Research Areas include: Stem cells; Neoangiogenesis; Molecular diagnostics; Polymerase chain reaction; In situ hybridization; DNA sequencing; Cell receptors; Carcinogenesis; Pathobiology of neoplasia; Complex infectious diseases; Transplantation; Cytokines; Flow cytomeric analysis; Inflammation; Cellular injury; Immunology and hypersensitivity; Athersclerosis.
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