Experimental Diabetes Research最新文献

Nontraditional Therapy of Diabetes and Its Complications 糖尿病及其并发症的非传统疗法

Experimental Diabetes Research Pub Date : 2021-01-29 DOI: 10.1155/2021/1592049

R. Zhao, A. Hui, Yong Xu, M. Rabijewski

引用次数: 1

In Vitro Investigation and Evaluation of Novel Drug Based on Polyherbal Extract against Type 2 Diabetes 复方提取物抗2型糖尿病新药的体外研究与评价

Experimental Diabetes Research Pub Date : 2020-03-19 DOI: 10.1155/2020/7357482

Zainab Riaz, M. Ali, Zunaira Qureshi, M. Mohsin

{"title":"In Vitro Investigation and Evaluation of Novel Drug Based on Polyherbal Extract against Type 2 Diabetes","authors":"Zainab Riaz, M. Ali, Zunaira Qureshi, M. Mohsin","doi":"10.1155/2020/7357482","DOIUrl":"https://doi.org/10.1155/2020/7357482","url":null,"abstract":"Background/Aim. Type 2 diabetes is the most common form of diabetes mellitus. The aim of this study was to develop and standardize the polyhedral formulation (granule) and check its efficacy with regard to type 2 diabetes. Methods. The alcoholic extract of each plant (H. antidysentrica, Prunus dulcis and Cicer arietinum) and oleic acid was mixed and then formulated by wet granulation method. FTIR was done to investigate the presence of active compounds. Physicochemical properties of granules were evaluated and antidiabetic potential was substantiated through inhibition of carbohydrate digestive enzyme (α-amylase and α-glucosidase), glucose uptake activity in yeast cells, and antioxidant activity. Results. IR spectra indicated the presence of active compounds by showing the characteristic peaks of phenols and amines. The FTIR results also showed no interaction between drug and excipients. The prepared granules exhibited excellent flow properties according to USP 30. The dissolution profile of active pharmaceutical ingredient (API) from granules showed 72–80% release in 2 hrs. Granules exhibited better inhibition of α-amylase and α-glucosidase as in comparison with the standard drug and found to be dose-dependent. The enhanced uptake of glucose was observed with a decrease in drug concentration. Moreover, the DPPH scavenging activity was high (98%) at 1 mg/ml. Conclusion. The stabilized formulation (granules) was formed and the presence of active compounds is responsible for better antidiabetic activity by inhibiting carbohydrate-digesting enzymes. Hence, it could lower the postprandial hyperglycemia and has the potential to be used for the treatment of type II diabetes after determining the dose regime.","PeriodicalId":12109,"journal":{"name":"Experimental Diabetes Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90777566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Prevalence and Risk Factors Associated with Type 2 Diabetes in Elderly Patients Aged 45-80 Years at Kanungu District 卡农古地区45-80岁老年患者2型糖尿病患病率及相关危险因素

Experimental Diabetes Research Pub Date : 2020-01-30 DOI: 10.1155/2020/5152146

D. Asiimwe, G. Mauti, R. Kiconco

{"title":"Prevalence and Risk Factors Associated with Type 2 Diabetes in Elderly Patients Aged 45-80 Years at Kanungu District","authors":"D. Asiimwe, G. Mauti, R. Kiconco","doi":"10.1155/2020/5152146","DOIUrl":"https://doi.org/10.1155/2020/5152146","url":null,"abstract":"Background. Type 2 diabetes is a worldwide disaster including in Uganda, specifically in Kanungu District which had a rise in diabetic patients in 2018/2019 as compared to the 2017/2018 financial year. This research was determined to access the prevalence and risk factors associated with type 2 diabetes on elderly patients aged 45-80 years attending Kanungu Health Centre IV, Kanungu District. Methods. A cross-sectional study was conducted among patients aged 45-80 years attending Kanungu Health Centre IV from June to August 2019. The prevalence of type 2 diabetes was determined by the blood sugar of patients. Questionnaires were used to collect data for factors associated with type 2 diabetes. Data were statistically analyzed using the statistical package for social sciences (SPSS) version 25 (SPSS Inc., USA) at . Results. The overall prevalence of type 2 diabetes was 18.7% among the tested patients. 22.8% of diabetic patients were females as 7.8% were males. The age group most affected by diabetes was 61-65 years. Alcoholism, smoking, body mass index (BMI), and family history were found to be significantly associated with type 2 diabetes at value < 0.05. Conclusion. There was a high prevalence of type 2 diabetes observed in this study compared to studies done in previous years which raise a public health concern. This study also found that females and patients aged 61-65 years were most affected by diabetes. Lastly, the presence of family history for diabetes, overweight, and being obese increases the chances of acquiring type 2 diabetes.","PeriodicalId":12109,"journal":{"name":"Experimental Diabetes Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79882218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 63

Erratum to “Circulating Levels of MicroRNA from Children with Newly Diagnosed Type 1 Diabetes and Healthy Controls: Evidence That miR-25 Associates to Residual Beta-Cell Function and Glycaemic Control during Disease Progression” “新诊断的1型糖尿病儿童和健康对照者的MicroRNA循环水平:miR-25与疾病进展过程中剩余β细胞功能和血糖控制相关的证据”的勘误

Experimental Diabetes Research Pub Date : 2012-12-02 DOI: 10.1155/2012/672865

L. Nielsen, Cheng Wang, K. Sørensen, C. Bang-Berthelsen, L. Hansen, M. L. Andersen, P. Hougaard, A. Juul, Chen-Yu Zhang, F. Pociot, H. Mortensen

引用次数: 10

Regulation of RAGE for attenuating progression of diabetic vascular complications. RAGE对糖尿病血管并发症进展的调节。

Experimental Diabetes Research Pub Date : 2012-01-01 Epub Date: 2011-10-29 DOI: 10.1155/2012/894605

Myat Thu Thu Win, Yasuhiko Yamamoto, Seiichi Munesue, Hidehito Saito, Dong Han, So Motoyoshi, Tarek Kamal, Takuro Ohara, Takuo Watanabe, Hiroshi Yamamoto

引用次数: 53

Pentoxifylline attenuates methionine- and choline-deficient-diet-induced steatohepatitis by suppressing TNF-α expression and endoplasmic reticulum stress. 己酮茶碱通过抑制TNF-α表达和内质网应激，减轻蛋氨酸和胆碱缺乏饮食引起的脂肪性肝炎。

Experimental Diabetes Research Pub Date : 2012-01-01 Epub Date: 2012-01-29 DOI: 10.1155/2012/762565

Min Kyung Chae, Sang Gyu Park, Sun-Ok Song, Eun Seok Kang, Bong Soo Cha, Hyun Chul Lee, Byung-Wan Lee

{"title":"Pentoxifylline attenuates methionine- and choline-deficient-diet-induced steatohepatitis by suppressing TNF-α expression and endoplasmic reticulum stress.","authors":"Min Kyung Chae, Sang Gyu Park, Sun-Ok Song, Eun Seok Kang, Bong Soo Cha, Hyun Chul Lee, Byung-Wan Lee","doi":"10.1155/2012/762565","DOIUrl":"https://doi.org/10.1155/2012/762565","url":null,"abstract":"Background: Pentoxifylline (PTX) anti-TNF properties are known to exert hepatoprotective effects in various liver injury models. The aim of this study was to investigate whether PTX has beneficial roles in the development of methionine- and choline-deficient-(MCD-) diet-induced NAFLD SD rats in vivo and TNF-α-induced Hep3B cells in vitro.Methods: SD Rats were classified according to diet (chow or MCD diet) and treatment (normal saline or PTX injection) over a period of 4 weeks: group I (chow + saline, n = 4), group II (chow + PTX), group III (MCD + saline), and group IV (MCD + PTX). Hep3B cells were treated with 100 ng/ml TNF-α (24 h) in the absence or presence of PTX (1 mM).Results: PTX attenuated MCD-diet-induced serum ALT levels and hepatic steatosis. In real-time PCR and western blotting analysis, PTX decreased MCD-diet-induced TNF-alpha mRNA expression and proapoptotic unfolded protein response by ER stress (GRP78, p-eIF2, ATF4, IRE1α, CHOP, and p-JNK activation) in vivo. PTX (1 mM) reduced TNF-α-induced activation of GRP78, p-eIF2, ATF4, IRE1α, and CHOP in vitro.Conclusion: PTX has beneficial roles in the development of MCD-diet-induced steatohepatitis through partial suppression of TNF-α and ER stress.","PeriodicalId":12109,"journal":{"name":"Experimental Diabetes Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/762565","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30447454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Association of FTO polymorphisms with early age of obesity in obese Italian subjects. 意大利肥胖受试者中FTO多态性与早期肥胖的关系

Experimental Diabetes Research Pub Date : 2012-01-01 Epub Date: 2012-02-20 DOI: 10.1155/2012/872176

Federica Sentinelli, Michela Incani, Federica Coccia, Danila Capoccia, Valentina Maria Cambuli, Stefano Romeo, Efisio Cossu, Maria Gisella Cavallo, Frida Leonetti, Marco Giorgio Baroni

{"title":"Association of FTO polymorphisms with early age of obesity in obese Italian subjects.","authors":"Federica Sentinelli, Michela Incani, Federica Coccia, Danila Capoccia, Valentina Maria Cambuli, Stefano Romeo, Efisio Cossu, Maria Gisella Cavallo, Frida Leonetti, Marco Giorgio Baroni","doi":"10.1155/2012/872176","DOIUrl":"https://doi.org/10.1155/2012/872176","url":null,"abstract":"Obesity is recognized as a major health problem worldwide. Genetic factors play a major role in obesity, and genomewide association studies have provided evidence that several common variants within the fat mass- and obesity-associated (FTO) gene are significantly associated with obesity. Very limited data is available on FTO in the Italian population. Aims of our study are to investigate: (1) the association of FTO gene SNPs rs9939609 and rs9930506 with body mass index (BMI) and obesity-related parameters in a large cohort (n = 752) of Italian obese subjects; (2) the association between the two FTO SNPs and age of onset of obesity. Our results demonstrate a strong association between FTO SNPs rs9939609 (P < 0.043) and rs9930506 (P < 0.029) with BMI in the Italian population. FTO rs9930506 was significantly associated with higher BMI in a G allele dose-dependent manner (BMI + 1.4 kg/m² per G allele). We also observed that the association with BMI of the two FTO variants varied with age, with the carriers of the risk alleles developing an increase in body weight earlier in life. In conclusion, our study further demonstrates a role of the genetic variability in FTO on BMI in a large Italian population.","PeriodicalId":12109,"journal":{"name":"Experimental Diabetes Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/872176","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30535263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Insulin resistance promotes early atherosclerosis via increased proinflammatory proteins and oxidative stress in fructose-fed ApoE-KO mice. 在果糖喂养的ApoE-KO小鼠中，胰岛素抵抗通过增加促炎蛋白和氧化应激促进早期动脉粥样硬化。

Experimental Diabetes Research Pub Date : 2012-01-01 Epub Date: 2012-03-07 DOI: 10.1155/2012/941304

Beatriz Cannizzo, Agustín Luján, Natalia Estrella, Carina Lembo, Montserrat Cruzado, Claudia Castro

{"title":"Insulin resistance promotes early atherosclerosis via increased proinflammatory proteins and oxidative stress in fructose-fed ApoE-KO mice.","authors":"Beatriz Cannizzo, Agustín Luján, Natalia Estrella, Carina Lembo, Montserrat Cruzado, Claudia Castro","doi":"10.1155/2012/941304","DOIUrl":"https://doi.org/10.1155/2012/941304","url":null,"abstract":"High fructose intake induces an insulin resistance state associated with metabolic syndrome (MS). The effect of vascular inflammation in this model is not completely addressed. The aim of this study was to evaluate vascular remodeling, inflammatory and oxidative stress markers, and atheroma development in high-fructose diet-induced insulin resistance of ApoE-deficient mice (ApoE-KO). Mice were fed with either a normal chow or a 10% w/v fructose (HF) in drinking water over a period of 8 weeks. Thereafter, plasma metabolic parameters, vascular remodeling, atheroma lesion size, inflammatory markers, and NAD(P)H oxidase activity in the arteries were determined. HF diet induced a marked increase in plasma glucose, insulin, and triglycerides in ApoE-KO mice, provoked vascular remodeling, enhanced expression of vascular cell-adhesion molecule-1 (VCAM-1) and matrix metalloprotease 9 (MMP-9) and enlarged atherosclerotic lesion in aortic and carotid arteries. NAD(P)H oxidase activity was enhanced by fructose intake, and this effect was attenuated by tempol, a superoxide dismutase mimetic, and losartan, an Angiotensin II receptor antagonist. Our study results show that high-fructose-induced insulin resistance promotes a proinflammatory and prooxidant state which accelerates atherosclerotic plaque formation in ApoE-KO mice.","PeriodicalId":12109,"journal":{"name":"Experimental Diabetes Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/941304","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30552021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 26

Plasma plasminogen activator inhibitor-1 is associated with end-stage proliferative diabetic retinopathy in the Northern Chinese Han population. 血浆纤溶酶原激活物抑制剂-1与中国北方汉族人群终末期增殖性糖尿病视网膜病变相关

Experimental Diabetes Research Pub Date : 2012-01-01 Epub Date: 2012-10-14 DOI: 10.1155/2012/350852

Ze-long Zhong, Song Chen

{"title":"Plasma plasminogen activator inhibitor-1 is associated with end-stage proliferative diabetic retinopathy in the Northern Chinese Han population.","authors":"Ze-long Zhong, Song Chen","doi":"10.1155/2012/350852","DOIUrl":"https://doi.org/10.1155/2012/350852","url":null,"abstract":"Objective: To identify predictors of end-stage proliferative diabetic retinopathy (PDR) in a cohort of individuals with type 2 diabetes mellitus (T2DM) from the Northern Chinese Han population.Methods: We investigated characteristics of 153 consecutive diabetic patients with end-stage PDR (62 males, 91 females), 123 consecutive PDR patients without end-stage PDR (48 males, 75 females), and 151 normal subjects (63 males, 88 females). Only one eye of each patient or healthy subject was included in this study. Univariate logistic regression models and multivariate logistic regression models were constructed to evaluate the predictors of end-stage PDR.Results: In univariate analysis, systolic blood pressure, diastolic blood pressure, duration of diabetes, family history of T2DM, and plasminogen activator inhibitor-1 (PAI-1) were significently associated with end-stage PDR. After multivariate analysis, family history of T2DM, plasma PAI-1 levels, smoking, and duration of diabetes were four positive predictors associated with end-stage PDR.Conclusions: Higher plasma levels of PAI-1 were associated with end-stage PDR in the Northern Chinese Han population with T2DM.","PeriodicalId":12109,"journal":{"name":"Experimental Diabetes Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/350852","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31147456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Investigating endothelial activation and oxidative stress in relation to glycaemic control in a multiethnic population. 研究内皮活化和氧化应激与多民族人群血糖控制的关系。

Experimental Diabetes Research Pub Date : 2012-01-01 Epub Date: 2012-12-06 DOI: 10.1155/2012/386041

E M Brady, D R Webb, D H Morris, K Khunti, D S C Talbot, N Sattar, M J Davies

{"title":"Investigating endothelial activation and oxidative stress in relation to glycaemic control in a multiethnic population.","authors":"E M Brady, D R Webb, D H Morris, K Khunti, D S C Talbot, N Sattar, M J Davies","doi":"10.1155/2012/386041","DOIUrl":"https://doi.org/10.1155/2012/386041","url":null,"abstract":"Aim: An exploration of ethnic differences in measures of oxidative stress and endothelial activation in relation to known cardiovascular risk factors within South Asians (SA) and White Europeans (WE) residing in the UK.Methods: 202 participants within a UK multiethnic population provided biomedical and anthropometric data. Human urinary 2,3-dinor-8-iso-prostaglandin-F1α and plasma ICAM-1 were quantified as measures of oxidative stress and endothelial activation, respectively.Results: 2,3-Dinor-8-iso-prostaglandin-F1α levels were significantly higher in the SA group compared to WE group (10.36 (95% CI: 9.09, 11.79) versus 8.46 (7.71, 9.29), P = 0.021) after adjustment for age, gender, smoking status, body weight, HbA1c, and medication. Oxidative stress was positively associated with HbA1c (β = 1.08, 95% CI:1.02, 1.14, P = 0.009), fasting (β = 1.06, 95% CI: 1.02, 1.10, P = 0.002), and 2 hr glucose (β = 1.02, 95% CI: 1.00, 1.04, P = 0.052). In each adjusted model, SA continued to have elevated levels of oxidative stress compared to WE. ICAM-1 levels were significantly higher in the composite IGR group compared to the normoglycaemic group (P < 0.001). No ethnic differences in ICAM-1 were observed.Conclusion: These results suggest that SA are more susceptible to the detrimental effects of hyperglycaemia-induced oxidative stress at lower blood glucose thresholds than WE. Further research into the potential mechanisms involved is warranted.","PeriodicalId":12109,"journal":{"name":"Experimental Diabetes Research","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/386041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31147457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11