{"title":"RNA-Seq Analysis Reveals DPYSL2 and PNKD Promotes Cardiomyocytes Differentiation during Reprogramming","authors":"Yaofei Wu","doi":"10.1145/3512452.3512458","DOIUrl":"https://doi.org/10.1145/3512452.3512458","url":null,"abstract":"Cardiomyocytes are terminally differentiated cells in the heart, whose injuries leads to severe health issue including cardiac failure and even death. Traditional medical procedure failed to rescue damaged cardiomyocytes and hence cannot cure severe myocardial damage. This is probably caused by the irreplaceability of limited cardiomyocytes number in our heart. Fortunately, recent studies reveal induced pluripotent stem cells (iPSCs) can be trained to differentiating into cardiomyocytes, opening the possibility of replacing damaged cardiomyocytes by reprogrammed iPSCs, though many remains unknow during cardiomyocytes reprogramming. In this project, we analyzed multiple RNA-Seq data during cardiomyocytes differentiation and reprogramming. We found potential candidate genes DPYSL2 and PNKD as well as several other genes that might play an important role in regulating cardiomyocytes reprogramming. We also proposed the possible mechanisms for these candidate genes based on their genetic nature as well as multi-omic data from public database. Hopefully, these studies could provide new insights on how cardiomyocytes differentiation were regulated during reprogramming and hence improving current iPSCs therapy for myocardial damage.","PeriodicalId":120446,"journal":{"name":"Proceedings of the 2021 5th International Conference on Computational Biology and Bioinformatics","volume":"162 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134422866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Overview: Some Basic Controversies and Applications of Cytochrome P450 Compound I","authors":"Zhi-Wen Xi","doi":"10.1145/3512452.3512464","DOIUrl":"https://doi.org/10.1145/3512452.3512464","url":null,"abstract":"Cytochrome P450 is a superfamily of enzymes that play an important role in body metabolism. Compound I has been identified to be a reactive intermediate for hydroxylation in Cytochrome P450 catalytic cycle. There are many controversies in the reactivity of other intermediates like Compound 0 and Compound II. Compound I was verified to be the main reactant to conduct hydrogen abstraction through comparative reactivity experiments. The oxygen rebound mechanism proposed by Groves in the catalytic cycle was proved to be a radical process by kinetics characterization. The structure of ferryl species has been widely studied in recent years. Apart from hydroxylation, Compound I can be involved in epoxidation, heteroatom oxidation, and decarboxylation when exposed to different substrates. Researchers also explore the possibilities of new products by replacing the components in the structure: the metal ion, the axial ligand, or the types of porphyrins.","PeriodicalId":120446,"journal":{"name":"Proceedings of the 2021 5th International Conference on Computational Biology and Bioinformatics","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132241005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Two New Purine Alkaloids-based Magnetic Organic Substances and Their Characterization","authors":"Qing He, L. Nie, Xueting Feng, S. Yao","doi":"10.1145/3512452.3512466","DOIUrl":"https://doi.org/10.1145/3512452.3512466","url":null,"abstract":"In this study, magnetic theophylline and adenine were synthesized and characterized. The characteristic peaks of anion appeared in the ultraviolet spectra, infrared spectra, mass spectra and Raman spectrum, which indicated that the target magnetic molecules were successfully synthesized. At the same time, the contents of C, H and N in the magnetic theophylline were determined by elemental analysis and compared with the theoretical values in order to verify the structures identified by spectral data. The results also showed that the synthesized magnetic theophylline had high purity. Moreover, the magnetic properties of two kinds of magnetic molecules were measured by using vibrating sample magnetometer (VSM) analysis, and the coercivity and other important data could be determined from the hysteresis loops. It was concluded that the two molecules were paramagnetic. Finally, micro-reaction system was initially developed in this study for synthesizing such kind of magnetic molecules.","PeriodicalId":120446,"journal":{"name":"Proceedings of the 2021 5th International Conference on Computational Biology and Bioinformatics","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125534490","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Proceedings of the 2021 5th International Conference on Computational Biology and Bioinformatics","authors":"","doi":"10.1145/3512452","DOIUrl":"https://doi.org/10.1145/3512452","url":null,"abstract":"","PeriodicalId":120446,"journal":{"name":"Proceedings of the 2021 5th International Conference on Computational Biology and Bioinformatics","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127945134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}