European Journal of Inflammation最新文献_第3页

STAT1 negatively regulates human glioma U251 cell proliferation Huang et al: Role of STAT1 in glioma STAT1负向调控人胶质瘤U251细胞增殖Huang等:STAT1在胶质瘤中的作用

4区医学

European Journal of Inflammation Pub Date : 2023-11-14 DOI: 10.1177/1721727x231214924

Ping Huang, Qiang Huang, Hongwei Wang, Changwu Dou, Haitao Ju, Rui Xiao, Lixia Zhang, Hailong Li

{"title":"STAT1 negatively regulates human glioma U251 cell proliferation Huang et al: Role of STAT1 in glioma","authors":"Ping Huang, Qiang Huang, Hongwei Wang, Changwu Dou, Haitao Ju, Rui Xiao, Lixia Zhang, Hailong Li","doi":"10.1177/1721727x231214924","DOIUrl":"https://doi.org/10.1177/1721727x231214924","url":null,"abstract":"Background Glioma is one of the most malignant tumors, which leads to high mortality in cancer patients. At present, there is no effective therapy for glioma. Therefore, it is urgent and necessary to find new molecular targets for anti-glioma therapy. Objective The present study aimed to investigate the role of signal transducer and activator of transcription 1 (STAT1) in the development and progression of human glioma and related mechanisms. Methods According to the instructions of Lipofectamine TM 2000 transfection reagent, we transiently transfected the plasmid pcDNA3.1-STAT1 into glioma U251 cells. Then STAT1 expression in glioma U251 and LN382 cells was detected by Western blot. MTT was performed to assay the proliferative activity of U251 cells after STAT1 transduction, flow cytometry was used to detect cell cycle and apoptosis indicators, cell migration indicator was determined by Wound healing, and Western blot was used for detecting the expression level and change trend of p53, p21, bcl-2, Caspase-8, Cyclin A and Cyclin E in transfected cells. Results Overexpressed STAT1 significantly inhibited U251 cell proliferation and promoted U251 cell apoptosis. Meanwhile, high expression of STAT1 can increase the expression of p53, p21, and Caspase-8 while inhibiting the expression of bcl-2, Cyclin A, and Cyclin E. Conclusion Highly expressed STAT1 inhibits the proliferative activity of human glioma U251 cells and can promote tumor cell apoptosis and block cell cycle progression while regulating the expression of various signal transduction molecules. Thus, STAT1 has a critical function in the development and progression of glioma and is a novel target for glioma therapy.","PeriodicalId":11913,"journal":{"name":"European Journal of Inflammation","volume":"16 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134957726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of circulating endothelial markers in COVID-19 patients admitted to the intensive care unit with laboratory data 重症监护病房入院COVID-19患者循环内皮标志物与实验室数据的相关性

4区医学

European Journal of Inflammation Pub Date : 2023-11-13 DOI: 10.1177/1721727x231210425

Waleed Hakami, Gasim Dobie, Abdullah A Mobarki, Aymen M Madkhali, Mohmmad S. Akhter, Abdulrahim R Hakami, Mohammed H Nahari, Yahya H Matari, Khaled Essawi, Ali Hakamy, Mohammad Algahtani, Denise E Jackson, Hassan A Hamali

{"title":"Correlation of circulating endothelial markers in COVID-19 patients admitted to the intensive care unit with laboratory data","authors":"Waleed Hakami, Gasim Dobie, Abdullah A Mobarki, Aymen M Madkhali, Mohmmad S. Akhter, Abdulrahim R Hakami, Mohammed H Nahari, Yahya H Matari, Khaled Essawi, Ali Hakamy, Mohammad Algahtani, Denise E Jackson, Hassan A Hamali","doi":"10.1177/1721727x231210425","DOIUrl":"https://doi.org/10.1177/1721727x231210425","url":null,"abstract":"Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is still a global concern with high morbidity and mortality rates. The role of endothelial cells in the progress of COVID-19 is well established. Therefore, the current study aimed to measure the endothelial markers and their correlation with the hematological parameters in intensive care unit-admitted COVID-19 patients. This study involved 111 adult participants, including 55 ICU-admitted patients with COVID-19 and 56 healthy controls. Levels of E-selectin, ICAM-1, and VCAM-1 in the plasma of the study participants were measured and correlated with hematological parameters. The study demonstrates that COVID-19 patients admitted to the ICU have higher levels of E-selectin, ICAM-1, and VCAM-1 compared to healthy controls ( p < .05). These elevated levels can serve as reliable indicators of endothelial dysfunction and early markers for the detection and prediction of endothelial cell involvement in COVID-19 complications. The findings of this study suggest that increased levels of E-selectin, VCAM-1, and ICAM-1 in patients with COVID-19 are indicative of the participation of endothelial cells in the pathogenesis of COVID-19 complications. Consequently, these endothelial markers are proposed as potential early indicators for predicting the severity of COVID-19.","PeriodicalId":11913,"journal":{"name":"European Journal of Inflammation","volume":"1 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136347867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights into the T-cell response to SARS-CoV-2 了解t细胞对SARS-CoV-2的反应

4区医学

European Journal of Inflammation Pub Date : 2023-11-03 DOI: 10.1177/1721727x231211458

Chaimae Kadi, Nouhaila Najimi, Menanne Zakaria, Bakri Youssef, Elmtili Noureddine, Seghrouchni Fouad

{"title":"Insights into the T-cell response to SARS-CoV-2","authors":"Chaimae Kadi, Nouhaila Najimi, Menanne Zakaria, Bakri Youssef, Elmtili Noureddine, Seghrouchni Fouad","doi":"10.1177/1721727x231211458","DOIUrl":"https://doi.org/10.1177/1721727x231211458","url":null,"abstract":"Following infection with SARS-CoV-2, cellular components of the adaptive immune system play a crucial role in eliminating the virus. Specifically, virus-specific CD4+ and CD8+ T cells generate effector cytokines and display cytotoxic activity. A number of studies carried out during the COVID-19 pandemic highlighted the importance of CD4+ T cells, CD8+ T cells, and memory cells in this process. T-cell responses emerge early and contribute to protection, but are comparatively impaired in severe cases, often accompanied by intense activation or lymphopenia. Since December 2020, SARS-CoV-2 vaccines have been licensed and administered worldwide. These vaccines induce a targeted T-cell response against SARS-CoV-2. The cellular response after the third dose was strong and superior to that obtained with the second dose. COVID-19 multiple vaccines elicit a robust CD4+ and CD8+ T cell response after the short-term booster. While, the T-cell response induced by COVID-19 vaccines has been shown to decline within 6-12 months of vaccination. In addition, the long-term persistence of cellular immunity may protect against the development of severe disease. In addition, adoptive T-cell therapies have shown considerable potential in the development of COVID-19 traitement. These therapies involve the transfer of T cells with specific antiviral properties into patients to boost their immune response against SARS-CoV-2.","PeriodicalId":11913,"journal":{"name":"European Journal of Inflammation","volume":"12 7","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135868074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of cigarette smoke exposure on rat’s spermatogenesis: A systematic literature review and meta-analysis 香烟烟雾暴露对大鼠精子发生的影响:系统文献综述和荟萃分析

4区医学

European Journal of Inflammation Pub Date : 2023-11-02 DOI: 10.1177/1721727x231207720

Tena Djuartina, Iskandar Rahardjo Budianto, Andreas Steven, Maria Stefani, Melani Kawilarang, Melisa Kawilarang

{"title":"The effect of cigarette smoke exposure on rat’s spermatogenesis: A systematic literature review and meta-analysis","authors":"Tena Djuartina, Iskandar Rahardjo Budianto, Andreas Steven, Maria Stefani, Melani Kawilarang, Melisa Kawilarang","doi":"10.1177/1721727x231207720","DOIUrl":"https://doi.org/10.1177/1721727x231207720","url":null,"abstract":"Objective: To conduct a systematic review and meta-analysis of all available published data related to rats about spermatogenesis to provide a comprehensive overview of the effects of cigarette smoke exposure on the parameters of spermatogenesis.Methods: A comprehensive search of electronic databases including PubMed, EBSCO, ProQuest, Science Direct, Taylor & Francis, Springer Link, GARUDA, and Google Scholar, spanning from their inception until January 29th, 2021, was conducted to retrieve relevant studies and full-text articles evaluating the effect of cigarette smoke exposure on the parameters of spermatogenesis in rats. Data were taken from eligible studies. We use SYRCLE’s Risk of Bias tool for this animal studies. The overall size effect was measured, and forest plots and funnel plots were generated using the statistical software JASP.Results: The present study comprised 23 studies involving 527 rats. The findings of this meta-analysis revealed that parameters of spermatogenesis, such as sperm count, morphology, and viability, were substantially impaired in response to conventional cigarette smoke exposure, exhibiting and aggregate correlation coefficient of 0,70 (95% CI:0.61-0.79, p < .001).Conclusion: Exposure to conventional cigarette smoke has a significant negative effect on spermatogenic parameters in rats, particularly in terms of sperm count, morphology, and viability.","PeriodicalId":11913,"journal":{"name":"European Journal of Inflammation","volume":"25 S3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135875565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Case report: Relapsed disseminated cutaneous herpes zoster successfully treated with brivudine 病例报告:布夫定成功治疗复发性播散性皮肤带状疱疹

4区医学

European Journal of Inflammation Pub Date : 2023-10-20 DOI: 10.1177/1721727x231209827

Mengdi Feng, Jinfang Zhang, Guoqiang Zhang

引用次数: 0

Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung Notch信号通路在纤维化养鸽人肺模型中调控巨噬细胞极化的作用

4区医学

European Journal of Inflammation Pub Date : 2023-09-28 DOI: 10.1177/1721727x231202431

Wenyi Wang, Yaoyuan Shen, Qiuping Leng, Wei Ding, Zhen Peng, Yuan Zhou, Xiaohong Yang, Chao Wu

{"title":"Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung","authors":"Wenyi Wang, Yaoyuan Shen, Qiuping Leng, Wei Ding, Zhen Peng, Yuan Zhou, Xiaohong Yang, Chao Wu","doi":"10.1177/1721727x231202431","DOIUrl":"https://doi.org/10.1177/1721727x231202431","url":null,"abstract":"Objectives: The Notch signaling pathway serves as the key regulator of the biological functions of macrophages and may affect the role of macrophages in the development of pigeon breeder’s lung (PBL). This study investigated the possible association of the Notch signaling pathway with the development of fibrotic PBL based on regulation of the polarization of macrophages. Methods: Normal Sprague‒Dawley rats and rats with pigeon exfoliation-induced fibrotic hypersensitivity pneumonitis were selected. DAPT was used to inhibit the Notch signaling pathway in rats, and pulmonary fibrosis, the Notch signaling pathway, macrophage polarization, and dynamic changes in the Th1/Th2 ratio were observed. Results: The levels of key proteins in the Notch signaling pathway were higher in the lung tissues of rats with fibrotic hypersensitivity pneumonitis than in those of normal controls ( p < 0.05). The mRNA expression levels of the M1 marker genes tumor necrosis factor (TNF)-α and inducible nitric oxide synthase (iNOS) were lower in the lung macrophages of rats with fibrotic hypersensitivity pneumonitis than in those of normal controls. In contrast, the mRNA expression levels of the M2 marker genes Mrc2 and Arg-1 in macrophages were higher in the rats with pneumonitis than in normal controls ( p < 0.05). The bronchoalveolar lavage fluid Th1/Th2 ratio was lower in the fibrotic hypersensitivity pneumonitis group than in the control group, but this trend was reversed DAPT application ( p < 0.05). Conclusion: Notch pathway receptors and ligands activate the inflammatory response of macrophages and participate in the polarization of macrophages to the M1 type. Inhibition of the Notch signaling pathway plays a role in Th1/Th2 imbalance.","PeriodicalId":11913,"journal":{"name":"European Journal of Inflammation","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135344422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The prognostic values of neutrophil to lymphocyte ratio in traumatically injured patients upon admission: A mini-Review 创伤性损伤患者入院时中性粒细胞与淋巴细胞比值的预后价值:一项小型综述

4区医学

European Journal of Inflammation Pub Date : 2023-09-27 DOI: 10.1177/1721727x231197494

Maryam Hosseini, Pooria Fazeli, Mahsa Hajivalili, Shahram Paydar

{"title":"The prognostic values of neutrophil to lymphocyte ratio in traumatically injured patients upon admission: A mini-Review","authors":"Maryam Hosseini, Pooria Fazeli, Mahsa Hajivalili, Shahram Paydar","doi":"10.1177/1721727x231197494","DOIUrl":"https://doi.org/10.1177/1721727x231197494","url":null,"abstract":"Subsequent to trauma and systemic inflammatory response syndrome, the typical reaction is an enhancement of the total white blood cell count. Neutrophils are abundant circulating leukocytes in humans that play a crucial role in initial immune response against invading microbes through phagocytosis and exerting inflammatory mediators. However, lymphocytes are the main cellular compartments of the immune system that are negatively affected in the setting of trauma. The neutrophil to lymphocyte ratio (NLR), which can be easily measured in daily clinical practices, is an alternative marker of inflammation before any clinical findings can be observed. Therefore, in this mini-review study, we briefly discussed recent evidence on NLR variations at the time of hospitalization and its prognostic values in trauma patients. Most investigations declared high values of NLR potentially have a poor prognosis in traumatically ill patients on admission and contribute to coagulopathy, increased hospitalization and mortality. Moreover, given that various cut-off points have been considered for the NLR value, receiving a unique one and linking with subsequent outcomes of the disease should be in ongoing researches.","PeriodicalId":11913,"journal":{"name":"European Journal of Inflammation","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135536827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Identification of potential biomarkers for nonsurvivor sepsis patients via microarray technology: A study based on GEO datasets 通过微阵列技术鉴定非存活败血症患者的潜在生物标志物:一项基于GEO数据集的研究

4区医学

European Journal of Inflammation Pub Date : 2023-09-23 DOI: 10.1177/1721727x231202661

Yan Ma, Shichao Shan, Cheng Luo, Jianlan Mo, Zhaokun Hu, Ren Jing

{"title":"Identification of potential biomarkers for nonsurvivor sepsis patients via microarray technology: A study based on GEO datasets","authors":"Yan Ma, Shichao Shan, Cheng Luo, Jianlan Mo, Zhaokun Hu, Ren Jing","doi":"10.1177/1721727x231202661","DOIUrl":"https://doi.org/10.1177/1721727x231202661","url":null,"abstract":"Background: The mechanism of sepsis especially non-survivors has not yet been identified. Objective: To identify the key genes concerned with non-survivor sepsis (NSS) and analyze its molecular mechanism. Methods: The original data were obtained from the GEO database to screen deferentially expressed genes (DEGs). GO and KEGG analysis were performed to analyze the functional annotation of DEGs. The protein-protein interaction (PPI) network and related analysis of hub genes were carried out. Further, hub genes were confirmed in the lipopolysaccharide (LPS)-induced septic mice by western blotting and immunohistochemistry. Results: We obtained 188 DEGs and 32 hub genes between NSS patients and healthy volunteers. Among of them, the top 10 hub genes including STAT1, ISG15, HERC1, EIF2AK2, RPL27, LY6E, IFI44L, XAF1, RSAD2 and HERC6 were studied, which predict sepsis based on receiver operator characteristic curve analysis. These hub genes were enriched in positive regulation of biomedical process including translation, response to virus and suppression of mitochondrial depolarization, etc.; cell component including mitochondrial inner membrane; molecular function containing ligase activity, etc. These hub genes are also enriched in influenza A infection and leukocyte trans-endothelial migration. The expression of these hub genes is better involved in a diagnosis of NSS, such as HERC6 (AUC = 0.9753, p = .0007), RPL27 (AUC = 0.9691, p = .0008), ISG15 (AUC = 0.9630, p = .0009), STAT1 (AUC = 0.9383, p = .0017), HERC1 (AUC = 0.9259, p = .0023), and XAF1 (AUC = 0.9259, p = .0023). Furthermore, 20 mg/kg of LPS injection up-regulated the expression of ISG15, RPL27, LY6E and HERC6 in the lung tissues compared with control mice. Conclusion: These identified 188 DEGs and 10 hub genes were associated with NSS, especially ISG15, RPL27, LY6E and HERC6 genes expressed in the lung as the most vulnerable organ.","PeriodicalId":11913,"journal":{"name":"European Journal of Inflammation","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135965247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-neutrophil cytoplasmic antibody associated vasculitis following rituximab: Outcomes of 50 patients in a tertiary single centre 利妥昔单抗后抗中性粒细胞细胞质抗体相关血管炎:三级单一中心50例患者的结果

4区医学

European Journal of Inflammation Pub Date : 2023-09-14 DOI: 10.1177/1721727x231202662

Fahidah Alenzi, Shirish R Sangle, David P D’Cruz

{"title":"Anti-neutrophil cytoplasmic antibody associated vasculitis following rituximab: Outcomes of 50 patients in a tertiary single centre","authors":"Fahidah Alenzi, Shirish R Sangle, David P D’Cruz","doi":"10.1177/1721727x231202662","DOIUrl":"https://doi.org/10.1177/1721727x231202662","url":null,"abstract":"Introduction: Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is an uncommon condition with heterogeneous multisystem organ involvement and significant morbidity and mortality. This study aimed to characterize the clinical features and laboratory characteristics, including B cell depletion, the ability to reduce corticosteroid dosage, and outcomes, of patients with AAV following rituximab treatment. Methods: Retrospective clinical and laboratory data were collected from patients with AAV who visited our lupus unit, including 50 treated with rituximab. Numeric response variables (median and range) were collected, including age, follow-up duration, disease duration, and Birmingham Vasculitis Activity Score (version 3). Statistical analyses were conducted using the SPSS 25.0 software. Statistical significance was considered a p-value <.05. Results: Of the 50 patients, 40 (80%) had granulomatosis with polyangiitis, 30 (75%) achieved remission, and 10 (25%) had active disease. Fifteen patients (30%) had positive ANCA levels at their last ANCA level assessment follow-up. Thirty-seven patients (74%) had B cell depletion, and 30 (81.1%) were in remission. Their median immunoglobulin levels were 7.6 (2.7–21.2) g/L for IgG, 0.5 (0.07–1.71) g/L for IgM, and 1.83 (0.14–4.87) g/L for IgA. Forty-two patients (84%) were able to lower their steroid dose to <7.5 mg, with 36 (85.7%) in remission and six (14.3%) having active disease ( p = .003). Conclusion: Our data suggests that most patients experience clinical remission after rituximab maintenance treatment. Half the patients were in remission, with normal creatinine levels and inflammatory markers. In addition, our patients could reduce steroid use.","PeriodicalId":11913,"journal":{"name":"European Journal of Inflammation","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134911741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metformin treatment of juvenile mice potentiates innate immune response to bacterial lipopolysaccharide in males 二甲双胍治疗幼鼠增强先天免疫反应的细菌脂多糖在雄性

4区医学

European Journal of Inflammation Pub Date : 2023-09-11 DOI: 10.1177/1721727x231199369

Yujie Sun, Yun Zhu, Andrzej Bartke, Lin Lin, Rong Yuan

{"title":"Metformin treatment of juvenile mice potentiates innate immune response to bacterial lipopolysaccharide in males","authors":"Yujie Sun, Yun Zhu, Andrzej Bartke, Lin Lin, Rong Yuan","doi":"10.1177/1721727x231199369","DOIUrl":"https://doi.org/10.1177/1721727x231199369","url":null,"abstract":"Objective: Metformin is a widely used drug, with a good safety profile, for treatment of type 2 diabetes. Recently, it has been found to also exhibit immunomodulatory potential. We aim to preliminarily explore the potential role of metformin in regulating innate immunity using UM-HET3 mice. Methods: In this study, we treated juvenile UM-HET3 mice with metformin and investigated the alteration of the innate immune response. Peripheral blood of HET3 mice was treated with ex-vivo lipopolysaccharide (LPS) stimulation to induce a leukocyte inflammatory response, which was detected by analyzing the expression of LPS receptors TLR4 and CD14 on leukocytes with flow cytometry. Furthermore, cytokine release induced by LPS was measured in isolated cell culture supernatants with ELISA. Results: In this study LPS treatment triggered a downregulation of TLR4 as well as an upregulation of CD14 expressed on the cell surface. Besides, it enhanced the secretion of pro-inflammatory cytokines TNF-α, IL-1β and IL-6. The results showed that metformin treatment led to a significant ( p < .05) increase in the expression of CD14 and pro-inflammatory cytokines, including IL-1β and IL-6, as well as increased proportion of TLR4 downregulation on the cell surface in response to LPS, specifically in males. Conclusions: This study indicates that treating juvenile mice with metformin enhances the innate immune response to bacterial endotoxin in males, but not in females.","PeriodicalId":11913,"journal":{"name":"European Journal of Inflammation","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135937853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0