Environmental Biology of Fishes最新文献_第3页

Isolation, Characterization, and Assessment of Probiotic Lactococcus lactis from the Intestinal Tract of Largemouth Bass (Micropterus salmoides) 大口黑鲈肠道益生菌乳酸乳球菌的分离、鉴定与评价

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-06-16 DOI: 10.3390/fishes10060291

Xiaoyu Chen, Jiaonan Zhang, Jiao‐Lin Zhang, Wenzheng Zou, Qingpi Yan

{"title":"Isolation, Characterization, and Assessment of Probiotic Lactococcus lactis from the Intestinal Tract of Largemouth Bass (Micropterus salmoides)","authors":"Xiaoyu Chen, Jiaonan Zhang, Jiao‐Lin Zhang, Wenzheng Zou, Qingpi Yan","doi":"10.3390/fishes10060291","DOIUrl":"https://doi.org/10.3390/fishes10060291","url":null,"abstract":"The health benefits associated with microbial species inhabiting aquatic animals have garnered increasing attention, as it is expected that the colonization and efficacy of native probiotic bacteria adapted to the internal environment of the target species will be more active than non-native bacteria. In this study, six isolates were obtained from the intestinal tract of largemouth bass. Three of these isolates demonstrated higher growth ability compared to the others and were further characterized using in vitro assays. Lactococcus lactis LBM15 was found to exhibit antibacterial activity against common pathogens affecting largemouth bass; the adhesion inhibition capabilities of the isolates were systematically evaluated through competitive, repulsive, and substitutive adhesion assays. The strain inhibited adhesion to all six tested pathogen strains, with competitive adhesion inhibition rates ranging from 42% to 54%, the highest of which was observed against V. anguillarum. Repulsive adhesion inhibition rates ranged from 27% to 55%, with the highest rate noted for Edwardsiella tarda. Additionally, substitutive adhesion inhibition rates were found to range from 48% to 76%, with the highest inhibition observed against Aeromonas hydrophila. Furthermore, LBM15 exhibited favorable antimicrobial susceptibility profiles, showing sensitivity to 21 antibiotics tested. Notably, safety assessment trials were performed exposing fish to LBM15 at a concentration of 1 × 109 CFU/mL by injection and at a concentration of 1 × 108 CFU/mL by feed administration. No clinical abnormalities, behavioral alterations, or mortality were documented in either exposure group, confirming the safety of LBM15 for application in aquaculture. The results suggested that LBM15 isolates from largemouth bass have potential for further investigation and possible application as probiotic candidates.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 6","pages":"291-291"},"PeriodicalIF":0.0,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2410-3888/10/6/291/pdf?version=1750082160","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Toxicity and Safety Assessment of Key Pesticides Used in Rice Fields on Rice Flower Carp (Procypris merus) 稻田主要农药对稻花鲤的毒性及安全性评价

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-05-25 DOI: 10.3390/fishes10060248

Qingjun Shao, Yongming Ruan, Ru Liang, R. Jin, Jin Zhang, Lin Xie, Yihebali Chi, Jianyang Xia, Pingyang Zhu

{"title":"Toxicity and Safety Assessment of Key Pesticides Used in Rice Fields on Rice Flower Carp (Procypris merus)","authors":"Qingjun Shao, Yongming Ruan, Ru Liang, R. Jin, Jin Zhang, Lin Xie, Yihebali Chi, Jianyang Xia, Pingyang Zhu","doi":"10.3390/fishes10060248","DOIUrl":"https://doi.org/10.3390/fishes10060248","url":null,"abstract":"Integrated rice–fish farming, crucial for sustainable agriculture, relies on the judicious use of pesticide. This study evaluates the toxicity of six common rice-field pesticides on Procypris merus (rice flower carp), a key species in these systems. We conducted acute and chronic toxicity tests, assessing survival, growth, oxidative stress (SOD, CAT, MDA, 8-OHdG), and neurotoxicity (AChE). Results revealed a spectrum of toxicity: abamectin and trifloxystrobin were highly toxic; pretilachlor was moderately so; and glufosinate-ammonium, triflumezopyrim, and thiazole zinc were low. Notably, triflumezopyrim induced significant oxidative stress and DNA damage, while all three low-toxicity pesticides inhibited AChE activity, indicating potential neurotoxicity. Despite these effects, all observed toxicities were reversible within 7–14 days. Considering that the tested concentrations exceeded typical field application rates, glufosinate-ammonium, triflumezopyrim, and thiazole zinc are deemed relatively safe for P. merus at recommended dosages. Our findings provide critical insights for optimizing pesticide selection in rice–fish farming, balancing pest control with ecological safety, thereby informing sustainable agricultural practices.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 6","pages":"248-248"},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2410-3888/10/6/248/pdf?version=1748152832","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Megalobrama amblycephala bcl2l13 Gene in Apoptosis and Autophagy 双头巨鲷bcl2l13基因在细胞凋亡和自噬中的作用

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-05-24 DOI: 10.3390/fishes10060247

Shuzhen Wang, Xiaoming Li, Danyang Wu, Zexia Gao, Hong Liu, Huanling Wang

{"title":"The Role of Megalobrama amblycephala bcl2l13 Gene in Apoptosis and Autophagy","authors":"Shuzhen Wang, Xiaoming Li, Danyang Wu, Zexia Gao, Hong Liu, Huanling Wang","doi":"10.3390/fishes10060247","DOIUrl":"https://doi.org/10.3390/fishes10060247","url":null,"abstract":"Bcl-2-like protein 13 (Bcl2l13) plays an important role in the cell apoptosis and mitochondrial autophagy of mammals. However, the role of bcl2l13 remains unclear in fish. Therefore, in this study, the function of Megalobrama amblycephala bcl2l13 gene in apoptosis and autophagy was investigated. The results showed that the overexpression of M. amblycephala bcl2l13 under hypoxic condition led to a reduction of reactive oxygen species (ROS), an increase in the expression levels of autophagy-related genes (p62, lc3, pink1), and a disruption of mitochondrial structure. However, deleting its transmembrane (TM) and Bcl-2 homology no (BHNo) domains decreased the P62 protein level, suggesting its essential role in autophagy. Furthermore, bcl2l13 overexpression inhibited cell proliferation and increased apoptosis. Additional studies revealed that the permeability of the mitochondrial permeability transition pore (mPTP) increased after overexpression of bcl2l13, but decreased upon deletion of the TM domain. Additionally, hypoxia led to elevated Bcl2l13 and P62 levels, and caused mitochondrial damage in M. amblycephala liver after 48 h of treatment. In conclusion, bcl2l13 may induce autophagy, inhibit cell proliferation and promote apoptosis, while its TM and BHNo domains play pivotal roles in these processes.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 6","pages":"247-247"},"PeriodicalIF":0.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2410-3888/10/6/247/pdf?version=1748096644","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147903708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (Pelteobagrus fulvidraco) Following Combined Oral Administration 盐酸小檗碱对黄颡鱼（Pelteobagrus fulvidraco）联合口服环丙沙星配置特性的影响及其作用机制

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-05-23 DOI: 10.3390/fishes10060245

Tianfu Zhong, Xiangxuan Du, Yueyan Chen, Yongtao Liu

{"title":"Effect of Berberine Hydrochloride on Disposition Characteristics of Ciprofloxacin Hydrochloride and Its Mechanism in Yellow Catfish (Pelteobagrus fulvidraco) Following Combined Oral Administration","authors":"Tianfu Zhong, Xiangxuan Du, Yueyan Chen, Yongtao Liu","doi":"10.3390/fishes10060245","DOIUrl":"https://doi.org/10.3390/fishes10060245","url":null,"abstract":"To investigate the effects and underlying mechanisms of the Chinese herbal medicine berberine hydrochloride (BBH) on the pharmacokinetics of the antibiotic ciprofloxacin hydrochloride (CIP) in yellow catfish (Pelteobagrus fulvidraco), this study established two experimental groups: CIP alone and CIP combined with BBH. After administering the two treatment groups, we analyzed the pharmacokinetic characteristics and tissue distribution of CIP in yellow catfish, as well as the differences in the expression levels of two key genes involved in drug disposition—ABCB4 (ATP-binding cassette subfamily B member 4, related to drug transport) and CYP3A40 (cytochrome P450 3A40, related to drug metabolism)—in the intestinal tract. The results demonstrated that co-administration of CIP and BBH increased the maximum concentration (Cmax) and area under the concentration–time curve (AUC) of CIP while reducing its total body clearance (CL/F). Regarding gene expression, the combined treatment significantly downregulated ABCB4 expression in the intestine at certain time points compared to CIP alone, whereas CYP3A40 expression showed a non-significant decreasing trend. These findings suggest that BBH may enhance the absorption of CIP in yellow catfish by suppressing ABCB4 expression, thereby improving therapeutic efficacy at the same dosage.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 6","pages":"245-245"},"PeriodicalIF":0.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2410-3888/10/6/245/pdf?version=1747991847","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Hepcidin Deficiency Disrupts Iron Homeostasis and Induces Ferroptosis in Zebrafish Liver Hepcidin缺乏破坏铁稳态并诱导斑马鱼肝脏铁下垂

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-05-21 DOI: 10.3390/fishes10050243

Mingli Liu, Mingjian Peng, Jingwen Ma, Ruiqin Hu, Qianghua Xu, Peng Hu, Liangbiao Chen

{"title":"Hepcidin Deficiency Disrupts Iron Homeostasis and Induces Ferroptosis in Zebrafish Liver","authors":"Mingli Liu, Mingjian Peng, Jingwen Ma, Ruiqin Hu, Qianghua Xu, Peng Hu, Liangbiao Chen","doi":"10.3390/fishes10050243","DOIUrl":"https://doi.org/10.3390/fishes10050243","url":null,"abstract":"Hepcidin is a key regulator of systemic iron homeostasis, which is essential for maintaining iron balance and cellular health. To investigate its role in zebrafish, we empolyed a hepcidin knockout model. Morphological and histological analyses revealed pale livers and significant iron accumulation in hep−/− zebrafish, particularly in liver, skin, and egg tissues. RNA sequencing identified 1,424 differentially expressed genes (DEGs) between wild-type (WT) and hep−/− zebrafish, with significant enrichment in pathways related to ferroptosis, fatty acid degradation, and heme binding. Western blot analysis showed reduced levels of key iron-related proteins, including GPX4, Fth1, and ferroportin (FPN), indicating impaired iron transport and increased oxidative stress. Gene Ontology (GO) and KEGG analyses highlighted disruptions in iron metabolism and lipid oxidation, linking iron overload to ferroptosis in the absence of hepcidin. These findings demonstrate that hepcidin deficiency leads to profound dysregulation of iron homeostasis, driving lipid peroxidation and ferroptosis in the zebrafish liver. Our study provides mechanistic insights into the molecular consequences of hepcidin loss, advancing our understanding of iron-related oxidative damage and its physiological impacts.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 5","pages":"243-243"},"PeriodicalIF":0.0,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2410-3888/10/5/243/pdf?version=1747837335","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Core–Shell Magnetic Gold Nanoparticles with Chitosan Coating as a SERS Substrate: A Rapid Detection Strategy for Malachite Green Contamination in Aquatic Foods 壳聚糖包覆的核壳磁性金纳米颗粒作为SERS基底：一种快速检测水产品中孔雀石绿污染的方法

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-05-11 DOI: 10.3390/fishes10050221

Yihui Yang, Tao Huang, Shu Hu, Ye Hang, Jiali Xing, Shengnan Zhan

{"title":"Core–Shell Magnetic Gold Nanoparticles with Chitosan Coating as a SERS Substrate: A Rapid Detection Strategy for Malachite Green Contamination in Aquatic Foods","authors":"Yihui Yang, Tao Huang, Shu Hu, Ye Hang, Jiali Xing, Shengnan Zhan","doi":"10.3390/fishes10050221","DOIUrl":"https://doi.org/10.3390/fishes10050221","url":null,"abstract":"In this study, we developed a novel SERS-active magnetic substrate (MBs@CS@AuNPs) for detecting malachite green (MG) in aquatic products, including shrimp, cod, and aquaculture water. The substrate combines chitosan-functionalized magnetic nanobeads with dense gold nanoparticles. It efficiently enriches MG through electrostatic and π–π interactions, generates high-density plasmonic hotspots for stable signals, and utilizes a superparamagnetic core to concentrate MG molecules. This design achieved an ultralow detection limit of 10−9 M for MG in aquaculture samples, with a linear range spanning from 10−3 to 10−10 M (R2 = 0.999). The substrate demonstrated superior performance in untreated, complex food matrices (e.g., shrimp, cod), outperforming conventional magnetic mass spectrometry systems that are prone to matrix interference. This work introduces an innovative approach for detecting harmful residues in food during environmental safety monitoring.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 5","pages":"221-221"},"PeriodicalIF":0.0,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2410-3888/10/5/221/pdf?version=1746971417","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147898002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Microplastics Enhance the Toxic Effects of Tetracycline on the Early Development of Zebrafish in a Dose-Dependent Manner 微塑料以剂量依赖的方式增强四环素对斑马鱼早期发育的毒性作用

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-03-27 DOI: 10.3390/fishes10040150

Yanqing Wu, Ziying Zhu, Riying Zhong, Xilin Fang, Xiaocui Wang, Yuanyin Huang, Han Gong, Muting Yan

{"title":"Microplastics Enhance the Toxic Effects of Tetracycline on the Early Development of Zebrafish in a Dose-Dependent Manner","authors":"Yanqing Wu, Ziying Zhu, Riying Zhong, Xilin Fang, Xiaocui Wang, Yuanyin Huang, Han Gong, Muting Yan","doi":"10.3390/fishes10040150","DOIUrl":"https://doi.org/10.3390/fishes10040150","url":null,"abstract":"Microplastic pollution in the environment has greatly increased due to the widespread use of plastics. Antibiotics and microplastic are common contaminants, especially in aquaculture. Microplastics could act as antibiotic vectors that raise the potential of their ecotoxicological effects. In this work, we conducted several analyses of biomarker responses to examine the developmental toxicity and toxicological endpoints that polyethylene microplastics (PE-MPs) and tetracycline antibiotics (TC) induced in zebrafish (Danio rerio) embryos/larvae. The results suggested that TC-PE-MPs induced significant physiological perturbations, including attenuated spontaneous cardiac contractions, cardiotoxicity, a dose-dependent elevation in mortality, and a marked reduction in body length, accompanied by morphological alterations. The mechanistic analysis revealed that ROS accumulation triggered enzymatic activity changes, which further induced aberrant vascular development, robust inflammatory responses, and dysregulated gene expression. These findings demonstrate that PE coexistence potentiates TC’s toxicological effects, with combined exposure inducing developmental toxicity during critical organogenesis stages in zebrafish. Overall, the current research demonstrated the detrimental effects of TC-PE-MPs on early fish development, suggesting potential environmental risks.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 4","pages":"150-150"},"PeriodicalIF":0.0,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2410-3888/10/4/150/pdf?version=1743163676","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Photoelectrocatalytic Coupling of Chlorine Radicals Enhances Sulfonamide Antibiotic Degradation in Saline-Alkaline Waters in Cold-Water Fish Aquaculture 氯自由基的光电催化偶联促进冷水鱼盐碱水中磺胺类抗生素的降解

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-03-06 DOI: 10.3390/fishes10030116

Qikai Liu, Yang Liu, Yaqi Mao, Ru Li, Yujie Jiao, Jiali Lei, Fenzhen Zhang

{"title":"Photoelectrocatalytic Coupling of Chlorine Radicals Enhances Sulfonamide Antibiotic Degradation in Saline-Alkaline Waters in Cold-Water Fish Aquaculture","authors":"Qikai Liu, Yang Liu, Yaqi Mao, Ru Li, Yujie Jiao, Jiali Lei, Fenzhen Zhang","doi":"10.3390/fishes10030116","DOIUrl":"https://doi.org/10.3390/fishes10030116","url":null,"abstract":"The degradation of organic matter using TiO2 nanotube photocatalytic technology is limited by the short lifetime and diffusion radius of the generated hydroxyl radicals, decreasing the removal efficiency. This study developed a chlorine radical-coupled photoelectrocatalytic system, significantly enhancing the performance of TiO2 nanotube arrays in removing sulfonamide antibiotics (SAs) from cold-water aquaculture systems. The highest degradation rates were observed at 5 mM NaCl and 15 mM NaNO3. When SA concentrations were 0.1–10 mg/L, degradation efficiency decreased with higher initial concentrations. The best degradation was achieved at an initial pH of 3 for SA. Humic acid and sodium acetate, natural organic matter in the water column, served as low-concentration promoters and high-concentration inhibitors. In our study, three degradation intermediates were identified, and hydrolysis and nitration reactions are proposed as the primary pathways for SA degradation. We confirmed that oxygen radicals play a major role in this system. Furthermore, toxicology experiments revealed the weakening of the toxicity of the degraded products. This study provides an efficient method for treating organic matter in cold-water fish culture water in chloride-containing saline and alkaline waters.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 3","pages":"116-116"},"PeriodicalIF":0.0,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2410-3888/10/3/116/pdf?version=1741254352","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147890820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Carbon Nanotube-Based Drug Delivery System Increases Drug Content and Promotes Immune Response in Mandarin Fish 基于碳纳米管的给药系统提高鳜鱼的药物含量并促进免疫反应

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-02-21 DOI: 10.3390/fishes10030092

Yijun Jia, Zhao Zhao, Leyang Chen, Yongqi Liu, Bin Zhu

{"title":"Carbon Nanotube-Based Drug Delivery System Increases Drug Content and Promotes Immune Response in Mandarin Fish","authors":"Yijun Jia, Zhao Zhao, Leyang Chen, Yongqi Liu, Bin Zhu","doi":"10.3390/fishes10030092","DOIUrl":"https://doi.org/10.3390/fishes10030092","url":null,"abstract":"A number of viral diseases have significantly impeded the growth of the aquaculture industry. Antiviral drugs represent an effective means of controlling infection. However, the efficacy of the entire therapeutic process is contingent upon the availability of an efficient delivery system. This study selected three common antiviral drugs and constructed corresponding drug delivery systems utilising single-walled carbon nanotubes (SWCNTs) as carriers. The reliability of carbon nanotubes as delivery carriers was evaluated by detecting the therapeutic effect on infectious splenic and renal necrosis virus (ISKNV). The findings demonstrated that SWCNTs can effectively enhance the absorption of the three drugs into the body, prolong their metabolic half-life, and improve the survival rate of fish infected with ISKNV. The Ribavirin-SWCNTs (RBV-SWCNTs) group exhibited the most pronounced protective effect, with a mortality rate of less than 25%. It was observed that SWCNTs facilitated the rapid transportation of ribavirin, with the drug content in the RBV-SWCNTs group being approximately double that of the free ribavirin group. Furthermore, this system markedly diminished the viral load, augmented enzyme activities, and elevated antiviral gene expression. This study indicated that carbon nanotubes are optimal carriers for antiviral drugs, which have considerable potential as a delivery vehicle for antiviral drugs to prevent viral infections in aquaculture.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 3","pages":"92-92"},"PeriodicalIF":0.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2410-3888/10/3/92/pdf?version=1740149515","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147332194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antiviral Effect and Metabolic Regularity of a Phenylpropanoid- Based Compound as Potential Immunopotentiator 潜在免疫增强剂苯丙类化合物的抗病毒作用和代谢规律

4区环境科学与生态学

Environmental Biology of Fishes Pub Date : 2025-02-15 DOI: 10.3390/fishes10020077

Dawei Song, Xue Cai, Qianhao Shao, Xin Tong, Zhe Zhao, Lei Liu, Guanglu Liu

{"title":"Antiviral Effect and Metabolic Regularity of a Phenylpropanoid- Based Compound as Potential Immunopotentiator","authors":"Dawei Song, Xue Cai, Qianhao Shao, Xin Tong, Zhe Zhao, Lei Liu, Guanglu Liu","doi":"10.3390/fishes10020077","DOIUrl":"https://doi.org/10.3390/fishes10020077","url":null,"abstract":"Spring viremia of carp virus (SVCV) is a significant pathogen that has notably hindered the advancement of cyprinid aquaculture in recent years. Infections caused by SVCV are often associated with substantial economic losses due to the absence of effective treatment options. Previous reports indicated that N-(4-methyl-2-oxo-2H-chromen-7-yl) benzenesulfonamide (N6) exhibits inhibitory effects on SVCV proliferation. This study aims to comprehensively evaluate the anti-SVCV effects of N6 using healthy young carp as the experimental model. The research investigates the antiviral activity of this compound in vivo, the immune response of interferon (IFN)-related genes, its impact on the horizontal transmission of SVCV, and histopathological changes. The results indicate that N6 significantly inhibits SVCV infectivity and apoptosis in EPC cells in vitro. Furthermore, while N6 reduced horizontal transmission of SVCV in a static cohabitation challenge model, the N6-treated SVCV-infected group showed a nearly 3-fold decrease in viral load compared to the control group, it did not completely prevent transmission at established antiviral dosages. Histopathological analysis of the affected fish revealed that N6 effectively mitigated tissue damage induced by SVCV. Additionally, the up-regulation of six IFN-related genes suggests that N6 may indirectly activate IFNs to facilitate the clearance of SVCV in the kidney and spleen, as demonstrated by quantitative reverse transcription polymerase chain reaction (qRT-PCR). These findings provide a foundation for further investigations into the mechanisms by which N6 acts against SVCV and may aid in the development of novel anti-SVCV therapeutics.","PeriodicalId":11799,"journal":{"name":"Environmental Biology of Fishes","volume":"10 2","pages":"77-77"},"PeriodicalIF":0.0,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147910213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1