European cytokine network最新文献_第4页

Interleukin-4-induced natural killer cell antitumor activity in metastatic melanoma patients. 白细胞介素-4诱导转移性黑色素瘤患者的自然杀伤细胞抗肿瘤活性

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-03 DOI: 10.1684/ecn.2020.0449

Ana M Vuletić, Gordana M Konjević, Annette K Larsen, Nada L Babović, Vladimir B Jurišić, Ana Krivokuća, Katarina M Mirjačić Martinović

{"title":"Interleukin-4-induced natural killer cell antitumor activity in metastatic melanoma patients.","authors":"Ana M Vuletić, Gordana M Konjević, Annette K Larsen, Nada L Babović, Vladimir B Jurišić, Ana Krivokuća, Katarina M Mirjačić Martinović","doi":"10.1684/ecn.2020.0449","DOIUrl":"10.1684/ecn.2020.0449","url":null,"abstract":"NK cells are important effectors of innate immunity that mount the first line of defense toward tumor growth. Interleukin-4 (IL-4) has recently been shown to regulate NK cell function, although its role in the regulation of NK cell function in cancer patients has not been clarified. The aim of this study was to investigate the effect of IL-4 on the function and the receptor characteristics of CD16-defined NK cells and their cytotoxic CD16bright and regulatory CD16dim subsets. Peripheral blood lymphocytes obtained from 36 metastatic melanoma (MM) patients treated for 18 h with 10 ng/mL IL-4 were evaluated for NK cell cytotoxicity using the radioactive 51chromium release assay. Expression of the activating receptors NKG2D and CD161, as well as the inhibitory receptors CD158a and CD158b, was analyzed on CD3-CD16+ NK cells and their subsets by flow cytometry. IL-4 induced significant in vitro enhancement of NK cell activity, as well as increased expression of the CD107a degranulation marker, by CD3-CD16dim NK cells. NKG2D expression was also increased on CD3-CD16+ cells by IL-4 with no alteration of the expression of CD161 and inhibitory KIR receptors. Although in vitro treatment with IL-4 increased both the expression of NKG2D and the cytotoxicity of NK cells, it had no detectable effect on the transcription of the TGF-β gene in NK cells of MM patients. The IL-4-induced NK cell cytotoxicity and increased activating NKG2D receptor expression may indicate an important antitumor effect of IL-4 with a potential application for immunotherapy of MM patients.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38670511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic targeting of interleukin-6 for the treatment of COVID-19. 以白细胞介素-6 为靶点治疗 COVID-19。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-03 DOI: 10.1684/ecn.2020.0453

Yao Wang, Chen Liu, Xiaolong Miao, Deqiang Kong, Yingli Zhao, Weihua Gong, Xianfeng Ding

{"title":"Therapeutic targeting of interleukin-6 for the treatment of COVID-19.","authors":"Yao Wang, Chen Liu, Xiaolong Miao, Deqiang Kong, Yingli Zhao, Weihua Gong, Xianfeng Ding","doi":"10.1684/ecn.2020.0453","DOIUrl":"10.1684/ecn.2020.0453","url":null,"abstract":"Coronavirus disease 19 (COVID-19), caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was first identified in China and has spread worldwide with a significant rate of infection. Considering the elevated levels of proinflammatory cytokines in COVID-19, it is suggested that cytokine storms play a critical role in its pathogenesis, including acute respiratory distress syndrome (ARDS). However, there is no specific drug for preventing the cytokine release syndrome (CRS) caused by COVID-19. Indeed, interleukin 6 (IL-6) has been highlighted for its many biological functions, such as immune regulation, inflammatory response, and metabolism. Therapeutic blockade of the IL-6 signaling pathway is expected to reduce the excessive immune reponse observed in COVID-19. Currently, the IL-6 receptor antagonists tocilizumab and sarilumab, have been adopted for preventing CRS during the progression of COVID-19, and remarkable beneficial effects were observed by using these humanized monoclonal antibodies. Based on the pathogenesis of COVID-19, we reviewed the biological mechanism of IL-6 blockade in the treatment of SARS-CoV-2 infection and evaluated its clinical applications.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38670514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High levels of proinflammatory cytokines IL-6, IL-8, TNF-Α, IL-23, and IFN- in Tunisian patients with type 1 autoimmune hepatitis. 突尼斯 1 型自身免疫性肝炎患者体内高水平的促炎细胞因子 IL-6、IL-8、TNF-Α、IL-23 和 IFN-。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-03 DOI: 10.1684/ecn.2020.0450

Maroua Chaouali, Mouna Ben Azaiez, Aymen Tezeghdenti, Besma Yacoubi-Oueslati, Ezzedine Ghazouani, Radhia Kochkar

{"title":"High levels of proinflammatory cytokines IL-6, IL-8, TNF-Α, IL-23, and IFN- in Tunisian patients with type 1 autoimmune hepatitis.","authors":"Maroua Chaouali, Mouna Ben Azaiez, Aymen Tezeghdenti, Besma Yacoubi-Oueslati, Ezzedine Ghazouani, Radhia Kochkar","doi":"10.1684/ecn.2020.0450","DOIUrl":"10.1684/ecn.2020.0450","url":null,"abstract":"Autoimmune hepatitis (AIH) is a chronic hepatitis of unknown etiology and several cytokines have been implicated in its pathogenesis and onset. Our objective was to determine the profile of pro and anti-inflammatory cytokines, including IL-1β, IL-6, IL-8, IL-23, IFN-, TNF-α, IL-10 in autoimmune hepatitis and their association with HLA gene polymorphisms. Serum cytokine levels were determined in 50 autoimmune hepatitis patients and one hundred fifty controls using chemiluminescence and ELISA techniques and HLA genotyping performed by PCR SSP. The levels of IL-6 (12 pg/mL vs. 5.5 pg/mL, p = 0.017), IL-8 (24.1 pg/mL vs. 7.8 pg/mL, p = 0.006), and TNF-α (61.1 pg/mL vs. <4.00 pg/mL, p = 0.002) were significantly higher in AIH patients in pretreatment phase compared to levels after remission and in controls. HLA*DRB15 was significantly associated with higher levels of IL-8. IL-6, IL-8, and TNF-α may be biomarkers of AIH activity. HLA gene expression may play a role in higher cytokine production and could allow an earlier diagnosis and better management of the disease.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38670510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preconceptional immunization with an allergen inhibits offspring thymic Th17 cells maturation without influence on Th1 and Th2 cells. 受孕前免疫一种过敏原会抑制后代胸腺 Th17 细胞的成熟，但不会影响 Th1 和 Th2 细胞。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-03 DOI: 10.1684/ecn.2020.0452

Marília Garcia de Oliveira, Fábio da Ressureição Sgnotto, Thamires Rodrigues de Sousa, Beatriz Oliveira Fagundes, Alberto José da Silva Duarte, Jefferson Russo Victor

{"title":"Preconceptional immunization with an allergen inhibits offspring thymic Th17 cells maturation without influence on Th1 and Th2 cells.","authors":"Marília Garcia de Oliveira, Fábio da Ressureição Sgnotto, Thamires Rodrigues de Sousa, Beatriz Oliveira Fagundes, Alberto José da Silva Duarte, Jefferson Russo Victor","doi":"10.1684/ecn.2020.0452","DOIUrl":"10.1684/ecn.2020.0452","url":null,"abstract":"The mechanisms through which maternal immunization can modulate offspring thymic maturation of lymphocytes are not fully understood. Here, we aimed to evaluate whether maternal OVA-immunization can inhibit the maturation of Th17 cells on offspring thymus. C57BL/6 females were immunized with OVA in Alum or Alum alone and mated with normal WT males. Offspring thymus was evaluated at three or 20 days of age. The demonstration that maternal OVA-immunization can inhibit offspring allergy development validated our experimental protocol. First, we observed that maternal OVA-immunization can inhibit the expression of R or γT and IL-17 molecules on immature T cells (CD4+CD8+) and TCD4 cells (CD4+CD8-) without interference on TCD8 cells (CD4-CD8+) on three-day-old offspring. A very similar effect could be observed on 20-day-old offspring. Additionally, a Th2 skewed profile could be found on the spleen of immunized pups from OVA-immunized mothers, but no influence was detected on offspring thymic Th1/Th2 profiles. Together, these data demonstrate that maternal immunization with an allergen can modulate offspring thymic maturation of Th17 cells without influencing Th1/Th2 patterns.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38669525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Absence of increased liver-related inflammatory markers in type 1 diabetes with metabolic syndrome: a nested case-control study from Brazil. 1型糖尿病伴代谢综合征患者肝脏相关炎症标志物缺失:一项来自巴西的巢式病例对照研究

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-01 DOI: 10.1684/ecn.2020.0459

Bianca Senger Vasconcelos Barros, Carlos Terra, Marilia Brito Gomes

{"title":"Absence of increased liver-related inflammatory markers in type 1 diabetes with metabolic syndrome: a nested case-control study from Brazil.","authors":"Bianca Senger Vasconcelos Barros, Carlos Terra, Marilia Brito Gomes","doi":"10.1684/ecn.2020.0459","DOIUrl":"https://doi.org/10.1684/ecn.2020.0459","url":null,"abstract":"To examine the relationship between metabolic syndrome and serum levels of interleukin (IL)-6 and IL-17, tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP), inflammatory biomarkers involved in nonalcoholic fatty liver disease (NAFLD) pathophysiology, in patients with type 1 diabetes.Methods: This was a cross-sectional, nested case-control study with 232 patients with type 1 diabetes (116 cases with metabolic syndrome and 116 controls without metabolic syndrome) who were matched for age and gender. A multivariable logistic regression with metabolic syndrome as the dependent variable was performed with inflammatory biomarkers and other parameters involved in NAFLD as independent variables.Results: Chronic kidney disease (CKD), retinopathy, body mass index (BMI), diabetes duration, alanine aminotransferase (ALT), fatty liver index (FLI), and CPR levels were associated with metabolic syndrome in univariate analysis. However, after adjustments in multivariable analysis, none of the liver-related inflammatory biomarkers persisted associated with metabolic syndrome. CKD, BMI, and ALT were associated with metabolic syndrome and retinopathy showed a tendency for association (p = 0.06).Conclusion: Although CRP, a nonspecific marker of inflammation, was associated with metabolic syndrome in univariate analysis, this fact did not persist after adjustments. No other inflammatory biomarkers showed an association with metabolic syndrome in type 1 diabetes. The group with metabolic syndrome had a higher frequency of diabetes' complications and markedly increased FLI. FLI probably is more useful in detecting NAFLD than inflammatory biomarkers, but further prospective studies in individuals with type 1 diabetes, with abdominal ultrasound and FLI, are necessary to better support this hypothesis.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25418359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin-27 gene variant rs153109 is associated with enhanced cytokine serum levels and susceptibility to Behçet's disease in the Iranian population. 在伊朗人群中，白细胞介素27基因变异rs153109与细胞因子血清水平升高和behet病易感性相关。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-01 DOI: 10.1684/ecn.2020.0458

Nasser Gholijani, Gholamreza Daryabor, Kurosh Kalantar, Mohammad-Reza Yazdani, Saeedeh Shenavandeh, Maryam Zahed, Zahra Jafarpour, Mohammad-Reza Malekmakan, Zahra Amirghofran

{"title":"Interleukin-27 gene variant rs153109 is associated with enhanced cytokine serum levels and susceptibility to Behçet's disease in the Iranian population.","authors":"Nasser Gholijani, Gholamreza Daryabor, Kurosh Kalantar, Mohammad-Reza Yazdani, Saeedeh Shenavandeh, Maryam Zahed, Zahra Jafarpour, Mohammad-Reza Malekmakan, Zahra Amirghofran","doi":"10.1684/ecn.2020.0458","DOIUrl":"https://doi.org/10.1684/ecn.2020.0458","url":null,"abstract":"Behcet's disease (BD) is a systemic vasculitis, characterized by recurrent oral aphthous, genital ulcers, ocular lesions, and other organ involvement. Interleukin (IL)-27 with its pro- and anti-inflammatory effects might be an important effective cytokine in this disease. The aim of this study was to investigate the association of IL-27 serum concentration and a single-nucleotide polymorphism (SNP) rs153109 (-964 A > G) with the risk and clinical features of the patients with BD. IL-27 Genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and the IL-27 serum levels were measured using enzyme-linked immunosorbent assay (ELISA). It is shown that AG, GG, and AG + GG genotypes, as well as G allele of rs153109, can significantly increase the risk of BD in total and in male individuals. Significantly higher frequencies of AG and GG genotypes and G allele were observed in total and male patients with an active form of BD. AG and GG genotypes were associated with joint (p = 0.046) and vascular (p = 0.02) involvement. The frequency of the G allele was higher in all patients, as well as in female patients with vascular involvement (p = 0.02). Serum cytokine analysis indicated an increased level of IL-27 in BD patients compared to healthy subjects (p = 0.038). Additionally, a higher level of IL-27 was detected in patients carrying the rs153109 GG genotype (p = 0.04) and those with renal (p = 0.009) and skin (p = 0.05) involvement. In conclusion, this study underscores the involvement of IL-27 rs153109 variants and increased serum level in BD susceptibility and pathogenesis.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25418358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Innate lymphoid cell subsets and their cytokines in autoimmune diseases. 自身免疫性疾病中的先天淋巴样细胞亚群及其细胞因子。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-01 DOI: 10.1684/ecn.2020.0460

Asma Maleki, Jamal Motallebzadeh Khanmiri, Mohammad Khani Eshrat Abadi, Faezeh Soveyzi, Bobak Moazzami, Michael R Hamblin, Nima Rezaei

引用次数: 2

Extensive longitudinal immune profiling reveals sustained innate immune activation in COVID-19 patients with unfavorable outcome 广泛的纵向免疫分析显示，COVID-19患者持续的先天免疫激活具有不利的结果

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-01 DOI: 10.1684/ecn.2020.0456

Benjamin Schrijver, Jorn L J C Assmann, Adriaan J van Gammeren, Roel C H Vermeulen, Lützen Portengen, Peter Heukels, Anton W Langerak, Willem A Dik, Vincent H J van der Velden, Ton A A M Ermens

{"title":"Extensive longitudinal immune profiling reveals sustained innate immune activation in COVID-19 patients with unfavorable outcome","authors":"Benjamin Schrijver, Jorn L J C Assmann, Adriaan J van Gammeren, Roel C H Vermeulen, Lützen Portengen, Peter Heukels, Anton W Langerak, Willem A Dik, Vincent H J van der Velden, Ton A A M Ermens","doi":"10.1684/ecn.2020.0456","DOIUrl":"https://doi.org/10.1684/ecn.2020.0456","url":null,"abstract":"COVID-19 differs substantially between individuals, ranging from mild to severe or even fatal. Heterogeneity in the immune response against SARS-COV-2 likely contributes to this. Therefore, we explored the temporal dynamics of key cellular and soluble mediators of innate and adaptive immune activation in relation to COVID-19 severity and progression. Forty-four patients with a PCR-proven diagnosis of COVID-19 were included. Extensive cellular (leukocytes and T-lymphocyte subsets) and serological immune profiling (cytokines, soluble cell surface molecules, and SARS-CoV-2 antibodies) was performed at hospital admission and every 3-4 days during hospitalization. Measurements and disease outcome were compared between patients with an unfavorable (IC admission and/or death) and favorable (all others) outcome. Patients with an unfavorable outcome had higher leukocyte numbers at baseline, mostly due to increased neutrophils, whereas lymphocyte and monocyte numbers were reduced. CRP, IL-6, CCL2, CXCL10, and GM-CSF levels were higher at baseline in the unfavorable group, whereas IL-7 levels were lower. SARS-CoV-2 antibodies were more frequently absent in the unfavorable group. Longitudinal analysis revealed delayed kinetics of activated CD4 and CD8 T-lymphocyte subsets in the unfavorable group. Furthermore, whereas CRP, IL-6, CXCL10, and GM-CSF declined in the favorable group, these cytokines declined with delayed kinetics, remained increased, or even increased further in the unfavorable group. Our data indicate a state of increased innate immune activation in COVID19-patients with an unfavorable outcome at hospital admission, which remained over time, as compared with patients with a favorable outcome.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7937051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25418360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Variations in IL-22, IL-27 and IL-35 serum levels in untreated and treated hepatitis C patients. 未经治疗和接受治疗的丙型肝炎患者血清中 IL-22、IL-27 和 IL-35 水平的变化。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-01 DOI: 10.1684/ecn.2020.0455

Azam Taghinejad, Shaghik Barani, Naser Gholijani, Farzad Ghandehari, Soolmaz Khansalar, Morvarid Asadipour, Mohammadali Davarpanah, Mohammadreza Fattahi, Kurosh Kalantar

{"title":"Variations in IL-22, IL-27 and IL-35 serum levels in untreated and treated hepatitis C patients.","authors":"Azam Taghinejad, Shaghik Barani, Naser Gholijani, Farzad Ghandehari, Soolmaz Khansalar, Morvarid Asadipour, Mohammadali Davarpanah, Mohammadreza Fattahi, Kurosh Kalantar","doi":"10.1684/ecn.2020.0455","DOIUrl":"10.1684/ecn.2020.0455","url":null,"abstract":"Background: Hepatitis C virus (HCV) is the leading cause of chronic liver diseases including hepatic fibrosis, cirrhosis, and hepatocellular carcinoma. We aimed to assess serum levels of interleukin (IL)-22, IL-27 and IL-35 in patients with hepatitis C and healthy controls to investigate their possible relationship with viral genotypes and liver enzyme levels.Method: A total of 30 newly diagnosed hepatitis C patients with no history of antiviral therapy and 30 healthy individuals participated in this study. Serum levels of IL-22, IL-27 and IL-35 were determined by ELISA in peripheral blood samples from patients prior to and following treament with pan-genotypic direct-acting anti-viral therapy. Serum levels of alanine transaminase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) were measured to determine any possible association between hepatic enzymes and cytokine serum levels concentrations.Result: The results show elevated serum levels of of IL-35 in HCV-infected patients compared to treated cases and healthy controls, whereas there was no significant difference in IL-22 and IL-27 serum levels among the three groups. Additionally, the cytokine levels were not significantly correlated with certain genotypes and levels of liver enzymes.Conclusion: Our findings indicate a potential role for IL-35 in chronic HCV infection and therapeutic management of patients with hepatitis C infection.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25418357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

BTK targeting suppresses inflammatory genes and ameliorates insulin resistance. BTK靶向抑制炎症基因并改善胰岛素抵抗。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-01 DOI: 10.1684/ecn.2020.0454

Mohammad Althubiti, Riyad Almaimani, Safaa Yehia Eid, Mohammad Elzubaier, Bassem Refaat, Shakir Idris, Turki Atia Alqurashi, Mahmoud Zaki El-Readi

{"title":"BTK targeting suppresses inflammatory genes and ameliorates insulin resistance.","authors":"Mohammad Althubiti, Riyad Almaimani, Safaa Yehia Eid, Mohammad Elzubaier, Bassem Refaat, Shakir Idris, Turki Atia Alqurashi, Mahmoud Zaki El-Readi","doi":"10.1684/ecn.2020.0454","DOIUrl":"https://doi.org/10.1684/ecn.2020.0454","url":null,"abstract":"Type 2 diabetes (T2D) causes profound psychological and physical distress to patients and burdens the health-care system. Although several antidiabetic drugs have been approved, none of them are adequately effective in the long-term management of T2D. Therefore, novel treatment options are needed for disease prevention or delaying disease progression. Bruton's tyrosine kinase (BTK) is a cytoplasmic enzyme that plays a role in B-cell differentiation and proliferation, and therapeutic targeting of BTK offers protection against chronic diseases. In this study, we analyzed BTK expression and its correlation with inflammatory mediators in patients with diabetes and obesity. The levels of BTK were significantly high in visceral adipose tissues of patients (p < 0.01) with diabetes and obesity compared with healthy controls. Additionally, a positive correlation was noted between the expression of BTK and the inflammatory cytokine genes TNF-α, INF-γ, IL-6, and IL-1 (p < 0.01) in adipose tissue. In insulin-resistant HepG2 cells (IR-HepG2), ibrutinib inhibited BTK expression in parallel with inflammatory genes, and increased insulin signaling and activity compared with untreated IR-HepG2 cells. Additionally, ibrutinib-treated IR-HepG2 cells showed increased glucose uptake compared with untreated IR-HepG2 cells. These results provide evidence that BTK inhibition may serve as a novel therapeutic strategy for the treatment of T2D. These findings also uncover the novel role of BTK in diabetes and insulin resistance; however, further in vivo studies are required prior to translating the findings into clinical settings.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25418361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6