European cytokine network最新文献_第4页

Serum Th17 and TNF-α distinguish between patients with occult hepatitis B infection, chronic hepatitis B infection and healthy individuals. 血清Th17和TNF-α是隐匿性乙型肝炎患者、慢性乙型肝炎患者和健康人的鉴别指标。

IF 2.8 4区医学

European cytokine network Pub Date : 2021-06-01 DOI: 10.1684/ecn.2021.0466

Francisca Sosa-Jurado, Laura Sánchez-Reza, Miguel Ángel Mendoza-Torres, Daniel Meléndez-Mena, Víctor Hugo García Y García, Belinda Guzmán-Flores, José Antonio Enciso-Moreno, Juan Ernesto López-Ramos, Juan Carlos Balandrán, Verónica Vallejo-Ruiz, Paulina Cortes-Hernández, Julio Reyes-Leyva, Gerardo Santos-López

{"title":"Serum Th17 and TNF-α distinguish between patients with occult hepatitis B infection, chronic hepatitis B infection and healthy individuals.","authors":"Francisca Sosa-Jurado, Laura Sánchez-Reza, Miguel Ángel Mendoza-Torres, Daniel Meléndez-Mena, Víctor Hugo García Y García, Belinda Guzmán-Flores, José Antonio Enciso-Moreno, Juan Ernesto López-Ramos, Juan Carlos Balandrán, Verónica Vallejo-Ruiz, Paulina Cortes-Hernández, Julio Reyes-Leyva, Gerardo Santos-López","doi":"10.1684/ecn.2021.0466","DOIUrl":"https://doi.org/10.1684/ecn.2021.0466","url":null,"abstract":"Chronic hepatitis B (CHB) is classified into five phases based on virus-host interactions: immune tolerance, immune clearance, inactive carrier state, reactive phase and occult hepatitis B infection (OBI). OBI is an uncommon asymptomatic phase of CHB that can be reactivated when the immune system is compromised, occasionally giving rise to severe liver disease. Host immune factors play essential roles in all phases of the CHB infection. Cytokines may alter infection course, influencing the propensity for and the progression of CHB and thus warrant study. Three clinical groups were studied: 48 healthy individuals (HI), 28 patients with persistent positive anti-HBc serological markers and negative HBsAg over time, who were diagnosed as OBI and 12 patients with active CHB. OBI patients were defined by three independent detections of the hepatitis B virus genome through nested PCR and real-time PCR. Quantitative measurement of 20 Th1, Th2 and Th17 human cytokines was performed in the sera of HI, OBI and CHB patients. Levels of IFN-γ, TNF-β, IL-28A, IL-4, IL-5, IL-13, IL-1β, IL-6, IL-21, IL-22, IL-23, GM-CSF and MIP-3α were similar between groups. IL-2, IL-12p70, IL-10, IL-17F and TGF-β1 were similar in HI and OBI, but higher in CHB. TNF-α and the IL-17A:IL-17F ratio were significantly different between the three groups. TNF-α was progressively higher in HI, OBI and CHB (P = 0.004), while the IL-17A:IL-17F ratio was 1.1 in HI, 3.4 in OBI and 0.4 in CHB. Detection and levels of these pro-inflammatory cytokines in OBI patients suggest that they are undergoing a silent hepatic inflammatory process.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"32 2","pages":"23-30"},"PeriodicalIF":2.8,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39302487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effect of white tea consumption on serum leptin, TNF-α and UCP1 gene expression in ovariectomized rats. 白茶摄入对去卵巢大鼠血清瘦素、TNF-α和UCP1基因表达的影响。

IF 2.8 4区医学

European cytokine network Pub Date : 2021-06-01 DOI: 10.1684/ecn.2021.0467

Sinan Saral, Faruk Saydam, Eda Dokumacioglu, Mehtap Atak, Levent Tümkaya, Hüseyin Avni Uydu

{"title":"Effect of white tea consumption on serum leptin, TNF-α and UCP1 gene expression in ovariectomized rats.","authors":"Sinan Saral, Faruk Saydam, Eda Dokumacioglu, Mehtap Atak, Levent Tümkaya, Hüseyin Avni Uydu","doi":"10.1684/ecn.2021.0467","DOIUrl":"https://doi.org/10.1684/ecn.2021.0467","url":null,"abstract":"Background: Obesity and dyslipidemia due to estrogen deficiency are among the important health problems in menopausal women. Increasing evidence reports the anti-obesity and anti-hyperlipidemic properties of tea polyphenols. However, the effect of white tea (WT) with high polyphenol content on overweight and lipid profile is uncertain. Here, we aimed to examine the effects of long-term WT consumption on serum leptin, tumor necrosis factor- alpha (TNF-α) and uncoupling protein 1 (UCP1) mRNA gene expression in ovariectomized (OVX) rats.Methods: Adult rats were divided into four groups (n = 8): (i) sham, (ii) OVX, (iii) WT and (iv) OVX + WT. WT was given at a dose of 0.5% w/v for 12 weeks. In the study, body weight, serum leptin, TNF, estradiol (E2) levels, lipid profile and UCP1 mRNA gene expression in brown adipose tissue (BAT) were evaluated.Results: There was a significant increase in body weight of OVX rats, which was decreased following WT consumption. While leptin and E2 levels decreased in the OVX group, TNF levels increased. There was no difference between the NF-kB levels of the groups. In addition, BAT UCP1 mRNA expression was significantly decreased in OVX groups, while WT treatment stimulated UCP1 activity.Conclusion: We explain the stimulatory effect of WT on weight loss mainly by the induction of UCP1 gene-mediated thermogenesis and suppression of inflammation. Therefore, we suggest that prolonged WT consumption may have beneficial effects in limiting excess weight gain caused by estrogen deficiency.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"32 2","pages":"31-38"},"PeriodicalIF":2.8,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39302488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

NLRP12 reduces proliferation and inflammation of rheumatoid arthritis fibroblast-like synoviocytes by regulating the NF-κB and MAPK pathways. NLRP12通过调节NF-κB和MAPK通路减少类风湿关节炎成纤维细胞样滑膜细胞的增殖和炎症。

IF 2.8 4区医学

European cytokine network Pub Date : 2021-06-01 DOI: 10.1684/ecn.2021.0465

Xin Zhang, He Nan, Jialong Guo, Jinyu Liu

{"title":"NLRP12 reduces proliferation and inflammation of rheumatoid arthritis fibroblast-like synoviocytes by regulating the NF-κB and MAPK pathways.","authors":"Xin Zhang, He Nan, Jialong Guo, Jinyu Liu","doi":"10.1684/ecn.2021.0465","DOIUrl":"https://doi.org/10.1684/ecn.2021.0465","url":null,"abstract":"Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease characterized by abnormal synovial hyperplasia and the release of inflammatory cytokines. NLRP12 is a member of the family nod-like receptor (NLR) families that are activators of inflammation. However, the role of NLRP12 in fibroblast-like synoviocytes (FLSs) is still unclear. In the present study, we have investigated the role of NLRP12 in fibroblast-like synoviocytes (FLSs). The results demonstrated that NLRP12 overexpression inhibited proliferation and promoted cell apoptosis in RA-FLSs. Moreover, NLRP12 overexpression repressed inflammation by downregulation of IL-1β, TNF-α, IL-6, IFN-γ and MCP-1 production and upregulation of IL-10 levels with knockdown of NLRP12 expression showing opposite effects. In addition, NLRP12 overexpression suppressed phosphorylation of JNK, ERK, p38 and NF-κB in RA-FLSs, whereas NLRP12 knockdown promoted phosphorylation of these proteins. In conclusion, these findings demonstrate that NLRP12 inhibits proliferation and inflammation of RA-FLSs via the regulation of the NF-κB and MAPK signaling pathways, suggesting that NLRP12 might be a potential target for RA treatment.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"32 2","pages":"15-22"},"PeriodicalIF":2.8,"publicationDate":"2021-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39302486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

GM-CSF-producing lymphocytes in tumor-draining lymph nodes of patients with bladder cancer. 膀胱癌患者排瘤淋巴结中产生gm - csf的淋巴细胞。

IF 2.8 4区医学

European cytokine network Pub Date : 2021-03-01 DOI: 10.1684/ecn.2021.0462

Ali Ariafar, Mohammadrasul Zareinejad, Mohammadreza Soltani, Yasmin Vahidi, Zahra Faghih

{"title":"GM-CSF-producing lymphocytes in tumor-draining lymph nodes of patients with bladder cancer.","authors":"Ali Ariafar, Mohammadrasul Zareinejad, Mohammadreza Soltani, Yasmin Vahidi, Zahra Faghih","doi":"10.1684/ecn.2021.0462","DOIUrl":"https://doi.org/10.1684/ecn.2021.0462","url":null,"abstract":"Bladder cancer (BC) is the tenth common cancer worldwide. Despite progress in treatment and the use of chemotherapoeutic drugs, the survival rate of BC patients is still low. Manipulation of the immune system was recently introduced as an interesting alternative treatment for this immunogenic cancer with fewer side effects. Accordingly, in the present study, we assessed the frequency of GM-CSF-producing lymphocytes in tumor-draining lymph nodes (TDLNs) of BC patients and evaluated their relationship with clinicopathological factors and survival rate. Fifty-four patients with BC who had received no treatment were recruited. Mononuclear cells were isolated from fresh homogenized lymph nodes by centrifugation over Ficoll-Hypaque, activated and subsequently analyzed by flow cytometry for the cell surface expression of CD4 and CD8 and the intracellular production of GM-CSF. Flow cytometric analysis revealed that 4.97 ± 2.7% of lymphocytes in TDLNs of patients with BC produced GM-CSF. The mean frequency of GM-CSF-producing cells was 5.5% among CD4+ lymphocytes and 11.7% in the CD8+ population. Elevated frequencies of GM-CSF-producing lymphocytes, as well as a higher production of GM-CSF by CD4+ lymphocytes was observed in the patients with tumor-free lymph nodes, as compared to those with at least one tumor-infiltrated lymph node (p < 0.05). On the other hand, the lower frequency of GM-CSF-producing CD4+ lymphocytes (ThGM) was associated with improved overall, but not one-year, survival. No other significant relationship was observed between clinicopathological parameters and the frequency of GM-CSF-producing subsets. Collectively, our findings suggest a protective role for GM-CSF in the early stages of BC; however, the unfavorable association of ThGM frequency with survival rate may imply a more complex role for this cytokine in BC.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"32 1","pages":"1-7"},"PeriodicalIF":2.8,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39276062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Interleukin 17 antagonist netakimab is effective and safe in the new coronavirus infection (COVID-19). 白细胞介素17拮抗剂奈他单抗对新型冠状病毒感染(COVID-19)有效且安全。

IF 2.8 4区医学

European cytokine network Pub Date : 2021-03-01 DOI: 10.1684/ecn.2021.0463

Roman Maslennikov, Vladimir Ivashkin, Ekaterina Vasilieva, Maxim Chipurik, Polina Semikova, Victoria Semenets, Tatyana Russkova, Anna Levshina, Diana Grigoriadis, Shamil Magomedov, Irina Efremova, Natiya Dzhakhaya

{"title":"Interleukin 17 antagonist netakimab is effective and safe in the new coronavirus infection (COVID-19).","authors":"Roman Maslennikov, Vladimir Ivashkin, Ekaterina Vasilieva, Maxim Chipurik, Polina Semikova, Victoria Semenets, Tatyana Russkova, Anna Levshina, Diana Grigoriadis, Shamil Magomedov, Irina Efremova, Natiya Dzhakhaya","doi":"10.1684/ecn.2021.0463","DOIUrl":"https://doi.org/10.1684/ecn.2021.0463","url":null,"abstract":"Cytokine release syndrome is a serious complication of the new coronavirus infection (COVID-19). The aim of the study was to assess effectiveness and safety of the IL-17 antagonist nekatimab for its treatment. The retrospective study included COVID-19 patients with C-reactive protein levels >60 mg/L. Patients received either netakimab (group NET), IL-6 antagonist tocilizumab (group TOC) or no anti-cytokine treatment (group CON). Forty-four patients were enrolled in the NET group, 27 patients in the TOC group, and 47 patients in the CON group. Mortality was lower in the NET group than in TOC and CON groups (2.3% vs. 14.8% and 31.9%; p = 0.018 and p < 0.001). NET group patients required intensive care unit admission (6.8% vs. 25.9% and 46.3%; p = 0.025 and p < 0.001) and mechanical ventilation (4.6% vs. 22.2% and 31.9%; p = 0.022 and p = 0.002) less frequently than patients of the TOC and CON groups. After 7-10 days of anti-cytokine drug administration, a reduction in lung lesion volume (p = 0.016) and an increase in the proportion of patients who did not need oxygen support (p = 0.005) or stayed in prone position (p = 0.044) was observed in the NET group only group; C-reactive protein levels were the same in the TOC and NET groups (p = 0.136) and lower in the CON group (p < 0.001 and p = 0.005). IL-6 levels decreased in the NET group (p = 0.005) and did not change in the TOC group (p = 0.953). There was no difference in the incidence of side effects between groups. The IL-17 antagonist netakimab is effective and safe in the treatment of cytokine release syndrome in COVID-19.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"32 1","pages":"8-14"},"PeriodicalIF":2.8,"publicationDate":"2021-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8491178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39276064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Interleukin-4-induced natural killer cell antitumor activity in metastatic melanoma patients. 白细胞介素-4诱导转移性黑色素瘤患者的自然杀伤细胞抗肿瘤活性

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-03 DOI: 10.1684/ecn.2020.0449

Ana M Vuletić, Gordana M Konjević, Annette K Larsen, Nada L Babović, Vladimir B Jurišić, Ana Krivokuća, Katarina M Mirjačić Martinović

{"title":"Interleukin-4-induced natural killer cell antitumor activity in metastatic melanoma patients.","authors":"Ana M Vuletić, Gordana M Konjević, Annette K Larsen, Nada L Babović, Vladimir B Jurišić, Ana Krivokuća, Katarina M Mirjačić Martinović","doi":"10.1684/ecn.2020.0449","DOIUrl":"10.1684/ecn.2020.0449","url":null,"abstract":"NK cells are important effectors of innate immunity that mount the first line of defense toward tumor growth. Interleukin-4 (IL-4) has recently been shown to regulate NK cell function, although its role in the regulation of NK cell function in cancer patients has not been clarified. The aim of this study was to investigate the effect of IL-4 on the function and the receptor characteristics of CD16-defined NK cells and their cytotoxic CD16bright and regulatory CD16dim subsets. Peripheral blood lymphocytes obtained from 36 metastatic melanoma (MM) patients treated for 18 h with 10 ng/mL IL-4 were evaluated for NK cell cytotoxicity using the radioactive 51chromium release assay. Expression of the activating receptors NKG2D and CD161, as well as the inhibitory receptors CD158a and CD158b, was analyzed on CD3-CD16+ NK cells and their subsets by flow cytometry. IL-4 induced significant in vitro enhancement of NK cell activity, as well as increased expression of the CD107a degranulation marker, by CD3-CD16dim NK cells. NKG2D expression was also increased on CD3-CD16+ cells by IL-4 with no alteration of the expression of CD161 and inhibitory KIR receptors. Although in vitro treatment with IL-4 increased both the expression of NKG2D and the cytotoxicity of NK cells, it had no detectable effect on the transcription of the TGF-β gene in NK cells of MM patients. The IL-4-induced NK cell cytotoxicity and increased activating NKG2D receptor expression may indicate an important antitumor effect of IL-4 with a potential application for immunotherapy of MM patients.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38670511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Therapeutic targeting of interleukin-6 for the treatment of COVID-19. 以白细胞介素-6 为靶点治疗 COVID-19。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-03 DOI: 10.1684/ecn.2020.0453

Yao Wang, Chen Liu, Xiaolong Miao, Deqiang Kong, Yingli Zhao, Weihua Gong, Xianfeng Ding

{"title":"Therapeutic targeting of interleukin-6 for the treatment of COVID-19.","authors":"Yao Wang, Chen Liu, Xiaolong Miao, Deqiang Kong, Yingli Zhao, Weihua Gong, Xianfeng Ding","doi":"10.1684/ecn.2020.0453","DOIUrl":"10.1684/ecn.2020.0453","url":null,"abstract":"Coronavirus disease 19 (COVID-19), caused by infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), was first identified in China and has spread worldwide with a significant rate of infection. Considering the elevated levels of proinflammatory cytokines in COVID-19, it is suggested that cytokine storms play a critical role in its pathogenesis, including acute respiratory distress syndrome (ARDS). However, there is no specific drug for preventing the cytokine release syndrome (CRS) caused by COVID-19. Indeed, interleukin 6 (IL-6) has been highlighted for its many biological functions, such as immune regulation, inflammatory response, and metabolism. Therapeutic blockade of the IL-6 signaling pathway is expected to reduce the excessive immune reponse observed in COVID-19. Currently, the IL-6 receptor antagonists tocilizumab and sarilumab, have been adopted for preventing CRS during the progression of COVID-19, and remarkable beneficial effects were observed by using these humanized monoclonal antibodies. Based on the pathogenesis of COVID-19, we reviewed the biological mechanism of IL-6 blockade in the treatment of SARS-CoV-2 infection and evaluated its clinical applications.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38670514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High levels of proinflammatory cytokines IL-6, IL-8, TNF-Α, IL-23, and IFN- in Tunisian patients with type 1 autoimmune hepatitis. 突尼斯 1 型自身免疫性肝炎患者体内高水平的促炎细胞因子 IL-6、IL-8、TNF-Α、IL-23 和 IFN-。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-03 DOI: 10.1684/ecn.2020.0450

Maroua Chaouali, Mouna Ben Azaiez, Aymen Tezeghdenti, Besma Yacoubi-Oueslati, Ezzedine Ghazouani, Radhia Kochkar

{"title":"High levels of proinflammatory cytokines IL-6, IL-8, TNF-Α, IL-23, and IFN- in Tunisian patients with type 1 autoimmune hepatitis.","authors":"Maroua Chaouali, Mouna Ben Azaiez, Aymen Tezeghdenti, Besma Yacoubi-Oueslati, Ezzedine Ghazouani, Radhia Kochkar","doi":"10.1684/ecn.2020.0450","DOIUrl":"10.1684/ecn.2020.0450","url":null,"abstract":"Autoimmune hepatitis (AIH) is a chronic hepatitis of unknown etiology and several cytokines have been implicated in its pathogenesis and onset. Our objective was to determine the profile of pro and anti-inflammatory cytokines, including IL-1β, IL-6, IL-8, IL-23, IFN-, TNF-α, IL-10 in autoimmune hepatitis and their association with HLA gene polymorphisms. Serum cytokine levels were determined in 50 autoimmune hepatitis patients and one hundred fifty controls using chemiluminescence and ELISA techniques and HLA genotyping performed by PCR SSP. The levels of IL-6 (12 pg/mL vs. 5.5 pg/mL, p = 0.017), IL-8 (24.1 pg/mL vs. 7.8 pg/mL, p = 0.006), and TNF-α (61.1 pg/mL vs. <4.00 pg/mL, p = 0.002) were significantly higher in AIH patients in pretreatment phase compared to levels after remission and in controls. HLA*DRB15 was significantly associated with higher levels of IL-8. IL-6, IL-8, and TNF-α may be biomarkers of AIH activity. HLA gene expression may play a role in higher cytokine production and could allow an earlier diagnosis and better management of the disease.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38670510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preconceptional immunization with an allergen inhibits offspring thymic Th17 cells maturation without influence on Th1 and Th2 cells. 受孕前免疫一种过敏原会抑制后代胸腺 Th17 细胞的成熟，但不会影响 Th1 和 Th2 细胞。

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-03 DOI: 10.1684/ecn.2020.0452

Marília Garcia de Oliveira, Fábio da Ressureição Sgnotto, Thamires Rodrigues de Sousa, Beatriz Oliveira Fagundes, Alberto José da Silva Duarte, Jefferson Russo Victor

{"title":"Preconceptional immunization with an allergen inhibits offspring thymic Th17 cells maturation without influence on Th1 and Th2 cells.","authors":"Marília Garcia de Oliveira, Fábio da Ressureição Sgnotto, Thamires Rodrigues de Sousa, Beatriz Oliveira Fagundes, Alberto José da Silva Duarte, Jefferson Russo Victor","doi":"10.1684/ecn.2020.0452","DOIUrl":"10.1684/ecn.2020.0452","url":null,"abstract":"The mechanisms through which maternal immunization can modulate offspring thymic maturation of lymphocytes are not fully understood. Here, we aimed to evaluate whether maternal OVA-immunization can inhibit the maturation of Th17 cells on offspring thymus. C57BL/6 females were immunized with OVA in Alum or Alum alone and mated with normal WT males. Offspring thymus was evaluated at three or 20 days of age. The demonstration that maternal OVA-immunization can inhibit offspring allergy development validated our experimental protocol. First, we observed that maternal OVA-immunization can inhibit the expression of R or γT and IL-17 molecules on immature T cells (CD4+CD8+) and TCD4 cells (CD4+CD8-) without interference on TCD8 cells (CD4-CD8+) on three-day-old offspring. A very similar effect could be observed on 20-day-old offspring. Additionally, a Th2 skewed profile could be found on the spleen of immunized pups from OVA-immunized mothers, but no influence was detected on offspring thymic Th1/Th2 profiles. Together, these data demonstrate that maternal immunization with an allergen can modulate offspring thymic maturation of Th17 cells without influencing Th1/Th2 patterns.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2020-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38669525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Absence of increased liver-related inflammatory markers in type 1 diabetes with metabolic syndrome: a nested case-control study from Brazil. 1型糖尿病伴代谢综合征患者肝脏相关炎症标志物缺失:一项来自巴西的巢式病例对照研究

IF 2.8 4区医学

European cytokine network Pub Date : 2020-12-01 DOI: 10.1684/ecn.2020.0459

Bianca Senger Vasconcelos Barros, Carlos Terra, Marilia Brito Gomes

{"title":"Absence of increased liver-related inflammatory markers in type 1 diabetes with metabolic syndrome: a nested case-control study from Brazil.","authors":"Bianca Senger Vasconcelos Barros, Carlos Terra, Marilia Brito Gomes","doi":"10.1684/ecn.2020.0459","DOIUrl":"https://doi.org/10.1684/ecn.2020.0459","url":null,"abstract":"To examine the relationship between metabolic syndrome and serum levels of interleukin (IL)-6 and IL-17, tumor necrosis factor-α (TNF-α), and C-reactive protein (CRP), inflammatory biomarkers involved in nonalcoholic fatty liver disease (NAFLD) pathophysiology, in patients with type 1 diabetes.Methods: This was a cross-sectional, nested case-control study with 232 patients with type 1 diabetes (116 cases with metabolic syndrome and 116 controls without metabolic syndrome) who were matched for age and gender. A multivariable logistic regression with metabolic syndrome as the dependent variable was performed with inflammatory biomarkers and other parameters involved in NAFLD as independent variables.Results: Chronic kidney disease (CKD), retinopathy, body mass index (BMI), diabetes duration, alanine aminotransferase (ALT), fatty liver index (FLI), and CPR levels were associated with metabolic syndrome in univariate analysis. However, after adjustments in multivariable analysis, none of the liver-related inflammatory biomarkers persisted associated with metabolic syndrome. CKD, BMI, and ALT were associated with metabolic syndrome and retinopathy showed a tendency for association (p = 0.06).Conclusion: Although CRP, a nonspecific marker of inflammation, was associated with metabolic syndrome in univariate analysis, this fact did not persist after adjustments. No other inflammatory biomarkers showed an association with metabolic syndrome in type 1 diabetes. The group with metabolic syndrome had a higher frequency of diabetes' complications and markedly increased FLI. FLI probably is more useful in detecting NAFLD than inflammatory biomarkers, but further prospective studies in individuals with type 1 diabetes, with abdominal ultrasound and FLI, are necessary to better support this hypothesis.","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"31 4","pages":"147-153"},"PeriodicalIF":2.8,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25418359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0