European cytokine network最新文献

{"title":"Seminal plasma adipokines: involvement in human reproductive functions.","authors":"Yaelle Elfassy,&nbsp;Chloe McAvoy,&nbsp;Soraya Fellahi,&nbsp;Joëlle Dupont,&nbsp;Bruno Fève,&nbsp;Rachel Levy,&nbsp;Jean-Philippe Bastard","doi":"10.1684/ecn.2018.0403","DOIUrl":"https://doi.org/10.1684/ecn.2018.0403","url":null,"abstract":"<p><p>Infertility, which increased worldwide over the past few decades, has recently been linked to obesity prevalence. Adipokines, produced by adipose tissue, could be the link between obesity and infertility. The association between circulating adipokines and female infertility has been extensively studied in the last ten years. However, the male aspect has been less investigated, although some adipokines are present in seminal plasma. We have attempted to analyze published studies that measured seminal plasma adipokines and their relationships with semen parameters. Apart from leptin, other seminal adipokines have rarely been studied. Indeed, leptin seems to have a differential role depending on its concentration in the seminal plasma. Thus, it could have a beneficial effect at lower concentrations but a deleterious effect at higher seminal levels. Although some studies are currently available, the roles of leptin and other adipokines in seminal plasma on sperm parameters and their consequences on male fertility remain to be clarified.</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 4","pages":"141-150"},"PeriodicalIF":2.8,"publicationDate":"2017-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2018.0403","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35861348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of interleukin 17A (IL-17A) neutralization on murine hepatitis virus (MHV-A59) infection.","authors":"José L Aparicio,&nbsp;Macarena Ottobre,&nbsp;Maite Duhalde Vega,&nbsp;Jean-Paul Coutelier,&nbsp;Jacques Van Snick,&nbsp;Lilia A Retegui","doi":"10.1684/ecn.2017.0399","DOIUrl":"https://doi.org/10.1684/ecn.2017.0399","url":null,"abstract":"<p><p>Mice infected with mouse hepatitis virus A59 (MHV-A59) develop hepatitis and autoantibodies (autoAb) to liver and kidney fumarylacetoacetate hydrolase (FAH), a fact closely related to the release of alarmins such as uric acid and/or high-mobility group box protein 1 (HMGB1). We studied the effect of neutralizing monoclonal antibodies (MAb) against IL-17A in our model of mouse MHV-A59-infection. MAb anti-IL-17F and anti-IFNγ were used to complement the study. Results showed that transaminase levels markedly decreased in MHV-A59-infected mice treated with MAb anti-IL-17A whereas plasmatic Ig concentration sharply increased. Conversely, MAb anti-IL-17F enhanced transaminase liberation and did not affect Ig levels. Serum IFNγ was detected in mice infected with MHV-A59 and its concentration increased after MAb anti-IL-17A administration. Besides, MAb anti-IFNγ greatly augmented transaminase plasmatic levels. IL-17A neutralization did not affect MHV-A59-induction of HMGB1 liberation and slightly augmented plasmatic uric acid concentration. However, mice treated with the MAb failed to produce autoAb to FAH. The above results suggest a reciprocal regulation of Th1 and Th17 cells acting on the different MHV-A59 effects. In addition, it is proposed that IL-17A is involved in alarmins adjuvant effects leading to autoAb expression.</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 3","pages":"111-119"},"PeriodicalIF":2.8,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2017.0399","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35600207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRR signaling during in vitro macrophage differentiation from progenitors modulates their subsequent response to inflammatory stimuli.","authors":"Alba Martínez,&nbsp;Cristina Bono,&nbsp;Javier Megías,&nbsp;Alberto Yáñez,&nbsp;Daniel Gozalbo,&nbsp;M Luisa Gil","doi":"10.1684/ecn.2017.0398","DOIUrl":"https://doi.org/10.1684/ecn.2017.0398","url":null,"abstract":"<p><p>Toll-like receptor (TLR) agonists drive hematopoietic stem and progenitor cells (HSPCs) to differentiate along the myeloid lineage in vitro and also in vivo following infection. In this study, we used an in vitro model of HSPC differentiation to investigate the functional consequences (cytokine production) that exposing HSPCs to various pathogen-associated molecular patterns (PAMPs) and Candida albicans cells have on the subsequently derived macrophages. Mouse HSPCs (Lin^- cells) were cultured with GM-CSF to induce macrophage differentiation in the presence or absence of the following pattern recognition receptor (PRR) agonists: Pam₃CSK₄ (TLR2 ligand), LPS (TLR4 ligand), depleted zymosan (which only activates Dectin-1), or inactivated C. albicans yeasts (which activate several PRRs, mainly TLR2 and Dectin-1). Our data show that only pure TLR2 ligand exposure (transient and continuous) impacts the inflammatory function of GM-CSF-derived macrophages, because Pam₃CSK₄-exposed HSPCs generate macrophages with a diminished ability to produce inflammatory cytokines. Interestingly, the Pam₃CSK₄-induced tolerance of macrophages (by transient exposure of HSPCs) is reinforced by subsequent exposure to C. albicans cells in GM-CSF-derived macrophages; however, the induced tolerance is partially reversed in M-CSF-derived macrophages. Therefore, the ability of macrophages to produce inflammatory cytokines is extremely dependent on how the HSPCs from which they are derived receive and integrate multiple microenvironmental signals (PRR ligands and/or CSFs).</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 3","pages":"102-110"},"PeriodicalIF":2.8,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2017.0398","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35600206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sophocarpine suppress inflammatory response in human fibroblast-like synoviocytes and in mice with collagen-induced arthritis.","authors":"Lihua Zhu,&nbsp;Liyan Zhu","doi":"10.1684/ecn.2017.0400","DOIUrl":"https://doi.org/10.1684/ecn.2017.0400","url":null,"abstract":"<p><p>Rheumatoid arthritis (RA) is a chronic autoimmune disease affecting nearly 1% of adults worldwide. This study aimed to investigate whether sophocarpine is a potential drug for treating RA. The cytotoxicity of sophocarpine to RA-fibroblast-like synoviocytes (FLSs) was evaluated using 3-[4,-dimethylthiazol-2-y]-2,5-diphenyl-tetrazolium bromide (MTT) assays kit and released lactate dehydrogenase (LDH) assays. The transcription of proinflammatory cytokines in RA-FLSs was analyzed by reverse transcription and real-time polymerase chain reaction (RT-PCR). The proteins levels were further verified by enzyme-linked immunosorbent assay (ELISA). The alterations in the mediators of mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) signaling pathways were tested by western blotting. The clinical effects of sophocarpine were evaluated in type II collagen-induced arthritis (CIA) in DBA-/1 mouse model by scoring their clinical responses, synovitis, and cartilage destructions, and ELISA was employed to analyze the concentrations of proinflammatory cytokines in the serum of CIA mice. The results showed that sophocarpine contained low cytotoxicity to RA-FLS cells, and it was capable to downregulate the expressions of LPS-induced proinflammatory cytokines. The suppressions of MAPK and NF-κB signaling pathways by sophocarpine were also found in LPS-induced RA-FLSs. The attenuation of the symptoms in CIA mouse model were significant, in which concentrations of proinflammatory cytokines were decreased after the sophocarpine treatment. In this study, we demonstrated the potential of sophocarpine in treating RA, both in vitro and in vivo. Sophocarpine may be a potential drug in treating human RA.</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 3","pages":"120-126"},"PeriodicalIF":2.8,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2017.0400","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35600649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzyme-Linked Immunosorbent Spot (ELISpot) monitoring of cytokine-producing cells for the prediction of acute rejection in renal transplant patients.","authors":"Fatemeh Mohammadi,&nbsp;Ghasem Solgi,&nbsp;Maryam Tajik,&nbsp;Pedram Ahmadpoor,&nbsp;Hasan Nikoeinejad,&nbsp;Behzad Einollahi,&nbsp;Banafsheh Nazari,&nbsp;Mahboob Lessan-Pezeshki,&nbsp;Aliakbar Amirzargar","doi":"10.1684/ecn.2017.0397","DOIUrl":"https://doi.org/10.1684/ecn.2017.0397","url":null,"abstract":"<p><p>The purpose of this study was to evaluate T-cell immunity markers using serial post-transplantation monitoring of cytokine-producing cells during the first post-transplant months for the prediction of acute rejection and potentially chronic rejection of kidney allograft. We followed 57 kidney allograft recipients for meanly 3 years post-transplantation. Blood samples were collected pre-transplant, 2, 4 and 12 weeks post-transplant. The frequencies of IL-10-, IL-17- and IFN-γ-producing cells were determined in all time-points using ELISPOT assay. The results of ELISpot monitoring and levels of IL-23 and TGF-β were compared between recipients with acute (n = 12) or chronic rejection episodes and patients with stable graft function (n = 45). In all post-transplant time-points, significantly high frequencies of IFN-γ- and IL-17-producing cells and low frequency of IL-10-producing cells were observed in rejection group versus patients with stable graft function (P < 0.0001). The ROC curve analysis for determining the reliability of cytokine-producing cells for the prediction of acute rejection revealed that AUC was 0.046 for IL-10 (P < 0.001), 0.927 for IL-17 (P < 0.001) and 0.929 for INF-γ-producing cells (P < 0.001). Our results indicate that analyzing the frequencies of INF-γ/IL-10/IL-17-producing cells may define a reliable panel for the prediction of acute rejection within the first post-transplant year which could also be applicable for the prediction of chronic rejection episodes.</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 3","pages":"93-101"},"PeriodicalIF":2.8,"publicationDate":"2017-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2017.0397","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35600650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skin innate immune response to flaviviral infection.","authors":"Magali Garcia,&nbsp;Michel Wehbe,&nbsp;Nicolas Lévêque,&nbsp;Charles Bodet","doi":"10.1684/ecn.2017.0394","DOIUrl":"https://doi.org/10.1684/ecn.2017.0394","url":null,"abstract":"<p><p>Skin is a complex organ and the largest interface of the human body exposed to numerous stress and pathogens. Skin is composed of different cell types that together perform essential functions such as pathogen sensing, barrier maintenance and immunity, at once providing the first line of defense against microbial infections and ensuring skin homeostasis. Being inoculated directly through the epidermis and the dermis during a vector blood meal, emerging Dengue, Zika and West Nile mosquito-borne viruses lead to the initiation of the innate immune response in resident skin cells and to the activation of dendritic cells, which migrate to the draining lymph node to elicit an adaptive response. This literature review aims to describe the inflammatory response and the innate immune signalization pathways involved in human skin cells during Dengue, Zika and West Nile virus infections.</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 2","pages":"41-51"},"PeriodicalIF":2.8,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2017.0394","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35445162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of host immune cells and Borrelia burgdorferi antigens in the etiology of Lyme disease.","authors":"Dennis Verhaegh,&nbsp;Leo A B Joosten,&nbsp;Marije Oosting","doi":"10.1684/ecn.2017.0396","DOIUrl":"https://doi.org/10.1684/ecn.2017.0396","url":null,"abstract":"<p><p>Lyme disease is a zoonosis caused by infection with bacteria belonging to the Borrelia burgdorferi species after the bite of an infected tick. Even though an infection by this bacterium can be effectively treated with antibiotics, when the infection stays unnoticed B. burgdorferi can persist and chronic post-treatment Lyme disease syndrome is able to develop. Although a cellular and humoral response is observed after an infection with the Borrelia bacteria, these pathogens are still capable to stay alive. Several immune evasive mechanisms have been revealed and explained and much work has been put into the understanding of the contribution of the innate and adaptive immune response. This review provides an overview with the latest findings regarding the cells of the innate and adaptive immune systems, how they recognize contribute and mediate in the killing of the B. burgdorferi spirochete. Moreover, this review also elaborates on the antigens that are expressed by on the spirochete. Since antigens drive the adaptive and, indirectly, the innate response, this review will discuss briefly the most important antigens that are described to date. Finally, there will be a brief elaboration on the escape mechanisms of B. burgdorferi with a focus on tick salivary proteins and spirochete antigens.</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 2","pages":"70-84"},"PeriodicalIF":2.8,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2017.0396","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35445165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR).","authors":"Maria Giovanna Vilia,&nbsp;Marta Tocchetti,&nbsp;Eleonora Fonte,&nbsp;Ilenia Sana,&nbsp;Marta Muzio","doi":"10.1684/ecn.2017.0395","DOIUrl":"https://doi.org/10.1684/ecn.2017.0395","url":null,"abstract":"<p><p>IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 2","pages":"63-69"},"PeriodicalIF":2.8,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2017.0395","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35445164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Buprenorphine differentially affects M1- and M2-polarized macrophages from human umbilical cord blood.","authors":"Juan Sun,&nbsp;Wei Guo,&nbsp;Xingguang Du","doi":"10.1684/ecn.2017.0392","DOIUrl":"https://doi.org/10.1684/ecn.2017.0392","url":null,"abstract":"<p><p>As a partial μ-opioid receptor agonist with long half-life time, buprenorphine has been widely used to relieve chronic cancer and nonmalignant pain. The maintenance of chronic pain involves inflammation; however whether buprenorphine has anti-inflammation property remains unclear. Macrophages, the immune cells that initiate and maintain inflammation, were isolated from human umbilical cord blood, and were polarized into M1 or M2 macrophages with IFN-γ in the presence of lipopolysaccharide (LPS) or IL-4, respectively. Quantitative PCR, ELISA, Western blotting analysis, and chromatin immunoprecipitation assays were employed to characterize M1 and M2 macrophages. 1) Buprenorphine did not change not only the apoptosis, survival, and morphology of resting macrophages, but also the antigen-presenting function of macrophages. 2) Buprenorphine inhibited the levels of mRNA and protein of several cytokines in M1 macrophages, and enhanced the expression of Ym1 and Fizz1 in M2 macrophages. 3) Buprenorphine did not affect the modulation of NF-κB and MAPK cascades by LPS in M1 macrophages. 4) Buprenorphine inhibited the expression of IRF5 and reduced binding of DNA to IRF5. Buprenorphine may downregulate IRF5 pathway and limit M1 macrophage phenotype. These effects may contribute to its therapeutic benefit for chronic neuropathic pain.</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 2","pages":"85-92"},"PeriodicalIF":2.8,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2017.0392","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35445166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predictive ability of circulating osteoprotegerin as a novel biomarker for early detection of acute kidney injury induced by sepsis.","authors":"Mona Schaalan,&nbsp;Waleed Mohamed","doi":"10.1684/ecn.2017.0393","DOIUrl":"https://doi.org/10.1684/ecn.2017.0393","url":null,"abstract":"<p><p>Though significant progress has been made towards new diagnostic approaches for early detection of acute kidney injury (AKI) induced by different factors, there is still an urgent demand for a more specific and predictive biomarker for each type. The aim of this study is to unravel the potential diagnostic utility of circulating osteoprotegerin (OPG) in septic patients who developed AKI in the ICU, compared to cystatin C (a renal function maker) and KIM-1 (a kidney damage marker). Eighty patients (male = 43, female = 37) with ages ranging from 42 to 46 years and with sepsis, 40 of whom developed AKI, and 30 healthy controls were enrolled in this prospective study. Results revealed significant progressive elevation of OPG, along with cystatin C and KIM-1, among sepsis, severe sepsis, and sepsis-AKI patients. The progression of OPG levels paralleled the deterioration of kidney and endothelial functions from sepsis to sepsis-AKI, revealed as progressively increased levels of serum E-selectin (15.3%), endothelin-1 (ET-1) (19.6%), and decreased nitric oxide (NO) (29.7%), associated with elevations of TNF-α (25.5%) and TGF-β (18%). Their comparative prognostic validity of sepsis-AKI was assessed using ROC analysis, which revealed that OPG, KIM-1, and cystatin C showed similar AUCs (0.827-0.83) but with different sensitivities, viz., 84%, 88%, and 92%, respectively. Although cystatin showed 82% specificity, OPG showed a higher, similar specificity to KIM-1 of 85%, indicating its potential function as a marker of renal damage such as KIM-1. This study revealed a significant elevation of circulating OPG in septic patients with different levels of severity and those who progressed to AKI. Moreover, OPG showed a significant correlation to KIM-1 and cystatin, as well as conventional renal, inflammatory, and endothelial markers. Having a similar specificity to KIM-1, as evidenced by the ROC analysis, OPG has the potential to serve as a reliable biomarker of kidney damage in cases of sepsis-AKI.</p>","PeriodicalId":11749,"journal":{"name":"European cytokine network","volume":"28 2","pages":"52-62"},"PeriodicalIF":2.8,"publicationDate":"2017-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1684/ecn.2017.0393","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35445163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}