Egyptian Journal of Radiation Sciences and Applications最新文献

{"title":"Histological and immunohistochemical evaluation for the effect of pilocarpine and quercetin on gamma-irradiated parotid salivary glands","authors":"S. Hani, Abd Elnasser Abd Elmawla, S. Ahmed, A. Emran","doi":"10.21608/EJRSA.2018.4820.1048","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.4820.1048","url":null,"abstract":"Radiotherapy of patients with head and neck tumors usually causes damage to the salivary glands since these are most frequently included in the field of irradiation. This study aims at investigating the possible protective effect of pilocarpine or quercetin against radiation induced parotid gland damage. Forty-five adult male rats were divided randomly into three main groups as follows: Radiation group where the rats were exposed to a single whole body 6Gy γ-irradiation; pilocarpine- and quercetin-radiation groups where the rats injected intrapretoneally by a single dose of pilocarpine (0.2mg/kg) or quercetin (1.25g/kg), respectively, 30min later exposed to a single whole body 6Gy γ-irradiation. The parotid glands were stained with hematoxylin and eosin and immunohistochemical reaction for vascular endothelial growth factor A (VEGF-A). Irradiated glands revealed massive acinar atrophy, degeneration and cytoplasmic vacuolization while, the ducts showed cytoplasmic vacuolizations with loss of regular cell architecture. Pilocarpine or quercetin treatment before radiation exposure offered some protection effect manifested as reduced acinaratrophy, degeneration and vacuolization. The ducts showed mild cellular vacuolizations. Regarding VEGF-A immunoreactivity, gamma irradiated parotid gland showed intense expression and significantly higher area that has persisted up to sixteen weeks. Pilocarpine or quercetin treatment was associated with lesser degree and significantly lower area percent of VEGF-A expressionas compared to the radiation group. Pilocarpine or quercetin has some improvement for the injurious effects of radiation on salivary glands so, they can be used as radioprotective agents before radiotherapy schedules to decrease radiation side effects on salivary glands.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76645674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RETRACTED ARTICLE: Thermoluminescence Characteristics of NaCl from Different Origins","authors":"S. Abdou, H. Amer, H. Abdel-ghany, Mohsen Albahar","doi":"10.21608/EJRSA.2018.3027.1041","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.3027.1041","url":null,"abstract":"","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74987901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of dnak, groES and cps genes in irradiated Klebsiella pneumoniae strains isolated from UTI Egyptian patients","authors":"H. Nada, S. Hagag, S. El-Tablawy","doi":"10.21608/EJRSA.2018.5656.1054","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.5656.1054","url":null,"abstract":"T his investigati on presents a brief study for monitoring the correlation between the expression of heat-shock protein (HSP) genes;  dnak, and groES as well as the capsular polysaccharide (caps) gene and antimicrobial susceptibility of extensively drug resistant (XDR)   Klebsiella pneumoniae in response to a low dose of Gamma (γ) radiation. The XDR K. pneumoniae strains, that were obtained from (UTI) Egyptian patients were identified using the API 20E strip automated system. Their antimicrobial susceptibilities were monitored using standard disc diffusion method in parallel with their minimum inhibition concentrations (MICs) towards standard antibiotics using a micro-titer method, before and after exposure to γ-radiation at a dose of 24.4 Gray (Gy). The expression of  dnak, groES, and cps genes was detected by qRT-PCR under the same conditions. The results demonstrate increasing antibiotic resistance after exposure to γ-radiation. Also, a doubling in the MIC of the tested strains towards CAZ(ceftazidime) was observed. These results were confirmed by an elevation in the expression of dnak, groES, and cps genes in response to γ-radiation. These preliminary results need more confirmaions, because they assume difficulties in curing immunocompromised patients infected with XDR K. pneumoniae by the currently available antibiotics.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72579835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulatory effect of irradiated β-glucan in Diethylnitrosamine induced Renal Toxicity","authors":"Gehan Moawed, Lobna A. Abdel-Aziz, Lobna M. Anees","doi":"10.21608/EJRSA.2018.4659.1047","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.4659.1047","url":null,"abstract":"β-glucans are one of the most abundant forms of polysaccharides known as biological response modifiers which influence host’s biological response and stimulate immune system. Accordingly, this study is carried out to evaluate the effect of gamma irradiated β-glucan (Iβ-glucan) extracted from mushroom in modulating diethylnitrosamine (DEN)-induced immune response and mitochondrial dysfunction in renal toxicity. Rats were divided into 4 groups. Group I: control group, group II: animals received the DEN (20mg/kg) for 6 weeks, group III: was gavaged Iβ-glucan (65mg/kg b. wt.) for 6 weeks, group IV: received as group II and then treated as group III. Exposure to DEN induced a change in the levels of cytokines (IFN-γ, TNF-α, IL-6) and nephrotoxicity, which was proved by significant elevated levels of creatinine and urea along with upregulation of mRNA cytochrome P450 (2E1) and downregulation in mitochondrial enzymes complex I and II. Treatment with Iβ-glucans post DEN-exposure significantly improved the disturbances in all the ++ tested parameters. It could be concluded that Iβ-glucan treatment exerts a potential biological effect on both mitochondrial and immune system dysfunction caused by the DEN- induced renal toxicity in rats. It is considered for further trials.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89338802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of VEGF expression in Systemic Lupus Erythematosus with and without nephritis","authors":"Dina Elessawi, Nashwa K. Radwan","doi":"10.21608/EJRSA.2018.6257.1056","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.6257.1056","url":null,"abstract":"T his work aims at evaluating the level of Vascular Endothelial Growth Factor (VEGF) in systemic lupus erythematosus (SLE) patients and studying its association with diseasing the activity and lupus nephritis(LN). The study was carried out on 40 SLE, 40 LN patients and 20 matched controls. Plasma levels of (VEGF) expression were measured using ELISA technique. Forty SLE and 40 LN patients with a mean age of (31.62±8.27) years while 20 healthy controls were (30.28±9.02) years and the SLE Disease Activity Index (SLEDAI) was (9.1±5.5). In the SLE patients, anti-nuclear antibodies (ANA) was positive in 47.5% and the anti-double stranded deoxyribonucleic acid (Anti-ds DNA) was positive in 52.5% of the patients while in LN (ANA) was positive in 47.5% of patients. Anti-ds DNA was positive in 62.5%. VEGA expression level was significantly increased in LN with mean (470.1±369.4U/ml) compared to the SLE patients with mean (183.54±95.03) with a p value (P=0.0001). A comparative analysis between immunological and biochemical data of the SLE patients and the lupus nephritis patients show an increase in the mean levels of ESR and creatinine in lupus nephritis patients compared to the SLE patients (P<0.005). VEGF level in the SLE patients is not associated with skin ulcer, Oral ulcers, malar rash, arthritis, proteinuria and anemia (P<0.05) while in the LN patients, it statistically significant increased with skin ulcer, Oral ulcers, malar rash, arthritis and anemia (P<0.05). It could be concluded that VEGF level is higher in the lupus nephritis group compared to the SLE without nephritis and control groups.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75101273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical impacts of treating diabetic rats with irradiated cinnamon","authors":"M. K. A. el-Megid, A. Mounir, A. Azeem, A. El-Shahat","doi":"10.21608/EJRSA.2018.6005.1055","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.6005.1055","url":null,"abstract":"T HE OBJECTIVES of the present study are to evaluate the effect of γ-radiation on total phenolic contents of cinnamon dried powder (CDP) and also to investigate the role of raw and γ-irradiated cinnamon extract (CE) in ameliorating biological disturbances in alloxan-induced diabetic rats. The results obtained show that γ-irradiation (10kGy) increases the total phenolic contents of CDP by percent change 6.39%. The results revealed also that alloxan administration to rats (150mg/kg B.WT) showed a significant increase in the levels of serum glucose, urea, creatinine, uric acidconcomitant with a significant decrease in the levels of insulin, testosterone (T), serum total thyroid hormones triiodothyronin (T3) and thyroxine (T4). Furtherer more, alloxan induced asignificant elevation in lipid peroxidation and a reduction in glutathione content and antioxidant enzyme activities in the renal and testicular tissues of diabetic rats. Treatment of diabetic rats with either raw or γ-irradiated CE has significantly ameliorated the alteration in the antioxidant/oxidant status and improved hyperglycemia, renal, and endocrine abnormalities. In conclusion, the study indicated that cinnamon, in addition to being hypoglycaemic, is effective in reducing oxidative stress caused by alloxan-induceddiabetes and the γ-irradiation of CPE increased its hygienic quality and significantly its total phenolic content without a significant loss in its quality attributes.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"693 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77037595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological and genetical study for the effect of gamma irradiation on Moringa olefiera Lam.","authors":"Hanan Mansour, N. Hamideldin, F. Abdel-Tawab, E. Fahmy, Hoda El Demerdash, M. Amar","doi":"10.21608/EJRSA.2018.2286.1035","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.2286.1035","url":null,"abstract":"SEEDS of Moringa oleifera plant were exposed to different gamma irradiation doses; 0, 20, 40 and 60Gy. Effects of gamma irradiation on a number of vegetative, physiological and molecular characters were studied. The vegetative characters were studied for two seasons, the highest values for the studied characters were often observed at the dose of 40Gy (8 characters) then at the dose of 60Gy (4 characters). The physiological characters studied were total phenolic compounds contents, amino acid contents and antioxidant enzymes activity. Total phenols contents showed a significant increase in all used doses compared with the control. Amino acids content was affected widely by gamma doses, especially with 20 and 40Gy.The effect of gamma irradiation on the activity of five antioxidant enzymes (catalase, polyphenol oxidase, peroxidase, superoxide dismutase and phenylalanine ammonia lyase) was studied. Isozymes electrophoresis was carried out for two isozymes (peroxidase and polyphenol oxidase). New bands were detected which can be used as markers for gamma irradiation.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"215 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75380325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impacts of Irradiated Distillers Dried Grains with Solubles (DDGS) as dietary Supplement on Physiological and Biochemical Aspects of Growing Rabbits","authors":"A. Azeem, U. Abdel-Monem, A. Mounir","doi":"10.21608/EJRSA.2018.3184.1042","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.3184.1042","url":null,"abstract":"D istillers dried grains with soluble (DDGS) are a co-product of ethanol production from starch cereals (mainly corn). Dietary fiber is the sum of carbohydrates and lignin that are resistant to digestion by the small intestine enzymes. DDGS contains approximately 3 \u0000times dietary fiber more than corn. Dietary fiber in DDGS consists mainly of insoluble dietary fiber. The present study was conducted to evaluate the effect of gamma (γ) irradiation on crude fiber, soluble fiber and non-soluble fiber of irradiated corn DDGs at different doses 0, 10, 20 and 30kGy, and to evaluate the effect of feeding irradiated DDGs for rabbits and their effects on rabbit’s performance (body weight, gain weight, feed intake, feed conversion, water intake, rectum temperature and respiration rate), apparent digestibility (dry matter, crude protein, crude fiber and ether extracts), carcass traits (carcass weight, dressing (%) and prime cuts (%) and blood biochemistry (AST, ALT , total protein, albumin, globulin, creatinine, total lipids, total cholesterol, and blood urea). Sixty New Zealand White weaned male rabbits of 35 days age were randomly divided to 4 treatment groups of approximately equal average weight (706g) with 15 \u0000animals each. The first group was fed the basal diet supplemented with 10% DDGS (non irradiated) (control), 2 nd, 3rd and 4th groups were fed the control diet supplemented with 10% irradiated DDGS at 10, 20 and 30kGy, respectively. The obtained results revealed a decrease in crude fiber and increases in soluble fiber and insoluble fiber. The increases were linearly correlated with the increasing irradiation dose. The supplemented irradiated 10% DDGS up to 30kGy in the diet of growing rabbits resulted in a significant improvement of body weight, gain weight, feed conversion, the apparent digestibility of dry matter, crude protein, crude fiber, carcass traits (carcass weight, dressing (%) and prime cuts(%)), and the improvement was \u0000parallel with increasing the radiation dose. While, feed intake, water intake, rectal temperature, respiration rate, ether extracts digestibility, AST, ALT , total protein, albumin, globulin, total lipids, total cholesterol, creatinine and blood urea were not altered by the treatments. Generally, it can be concluded that radiation processing with γ-rays improved the soluble and insoluble fiber and the digestibility of DDGS, and that DDGS supplemented diets fed to growing rabbits improved rabbits' growth performance without any deleterious effect on physiological and biochemical attributes.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"665 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77032385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ESR Studies on Characteristic Chemical Effects of Some Irradiated Antibiotics","authors":"S. Abdou, S. Ebraheem","doi":"10.21608/EJRSA.2018.3021.1040","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.3021.1040","url":null,"abstract":"MOST of the characteristic chemical effects of the irradiated solid drugs are not accurately detected by the conventional tests such as ultra violet spectro-photometry and melting point technique. Sterilization using gamma irradiation is a desirable alternative for the thermo-sensitive drugs. Antibiotics samples were -irradiated at room temperature with doses ranging from 2 kGy up to 25 kGy. Electron Spine Resonance (ESR) measurements were applied on four types of antibiotics; Amoxycillin, Ampicillin, Dalacin and Spiramycin (AMX, AMP, DAL and SPI). ESR results exhibited the impact of irradiation and storage parameters on the nature and concentration of the trapped radicals. The radical concentration is directly proportional to the ESR peak height signal also depending on the absorbed dose delivered to the antibiotic samples. The obtained information about the irradiated radiolytic products was found to verify the radical mechanisms. This information may be used to detect an irradiation treatment on such pharmaceuticals.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87009656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concerted effects of L-carnitine and vitamin E on cardio-pulmonary apoptosis induced by gamma irradiation in rats","authors":"Heba H. Mansour, S. Galal, S. M. kiki","doi":"10.21608/EJRSA.2018.4491.1046","DOIUrl":"https://doi.org/10.21608/EJRSA.2018.4491.1046","url":null,"abstract":"Pulmonary fibrosis and cardiotoxicity are a common and dose-limiting side-effect of ionizing radiation used to treat cancers of the thoracic region. This study aims at investigating the role of Bcl-2, Bax protein and caspase-3 in the pathogenesis of tissue damage induced by gamma irradiation (IRR) and the protective potential role of vitamin E (Vit E) and/or L-carnitine (L-car) against γ-irradiation induced oxidative injury. Irradiation was performed as a whole body γ-irradiation (5Gy). Immnuohistochemistry was used to estimate Bcl-2 expression. The level of Bax and caspase 3 activity were determined using enzyme-linked immunosorbent assay. C-reactive protein was determined using immunoturbidimetric assay. Results revealed that γ-irradiation (5Gy) induced apoptosis via the mitochondrial pathway by downregulating Bcl-2 expression, upregulating Bax protein and activating caspase-3. Administration of Vit E (50mg/kg) and/or L-car (300mg/kg) prior to gamma-irradiation decreased apoptosis through changing the expression of Bcl-2, Bax and caspase-3 activity. Exposure to γ-irradiation increased triglyceride, cholesterol, low density lipoprotein-cholesterol (LDL-C), C-reactive protein (CRP) and potassium levels, creatine kinase (CK-MB) and lactate dehydrogenase (LDH) activities and decreased high density lipoprotein-cholesterol (HDL-C) levels. The altered lipid profile, CRP and potassium levels, CK-MB and LDH activities induced by exposure to γ-irradiation were significantly renovated in Vit E and/or L-car pretreated γ-irradiated rats. In conclusion, Vit E and/or L-car might ameliorate γ-irradiation induced apoptosis via modulation of Bcl-2, Bax and caspase-3.","PeriodicalId":11658,"journal":{"name":"Egyptian Journal of Radiation Sciences and Applications","volume":" 28","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91413521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}