Endocrine regulations最新文献

{"title":"Metabolic disorders during endogenous Cushing's syndrome: prevalence, associated factors, and outcome after remission.","authors":"Melika Chihaoui,&nbsp;Ibtissem Oueslati,&nbsp;Nadia Khessairi,&nbsp;Fatma Chaker,&nbsp;Skander Cherni,&nbsp;Bessam Hammami,&nbsp;Moncef Feki,&nbsp;Meriem Yazidi","doi":"10.2478/enr-2023-0017","DOIUrl":"https://doi.org/10.2478/enr-2023-0017","url":null,"abstract":"Abstract Objective. The prognosis of Cushing’s syndrome (CS) is related to a higher cardiovascular morbidity and mortality. This study aimed to determine the prevalence of metabolic disorders in patients with CS, the associated factors, and the rate of remission of these disorders after the remission from CS. Methods. It is a retrospective study including 75 cases of CS followed up at the university hospital La Rabta of Tunis from 1987 to 2018. Clinical and paraclinical data were collected from medical files. Results. The mean age of the patients was 44.1±18.9 years and the sex ratio was 0.39. At CS diagnosis, the frequencies of obesity, hypertension, diabetes, dyslipidemia, and metabolic syndrome were 52, 75, 43, 83, and 73%, respectively. The age, gender, body mass index, waist circumference, and baseline serum cortisol level were not associated with the presence of diabetes, hypertension or dyslipidemia. Forty-eight patients were operated on. At one year, 38 patients were in remission from CS. The remission rates of hypertension, diabetes, and dyslipidemia were respectively 58% (p<0.001), 76% (p<0.001), and 17% (NS). Conclusion. Metabolic disorders were frequent during CS and their frequencies decreased after the remission from the syndrome.","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"138-143"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10250753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An index to prevent major limb amputations in diabetic foot.","authors":"Bugra Zengin,&nbsp;Burak Yuzuguldu,&nbsp;Ilgin Yildirim Simsir,&nbsp;Sevki Cetinkalp","doi":"10.2478/enr-2023-0010","DOIUrl":"https://doi.org/10.2478/enr-2023-0010","url":null,"abstract":"<p><p><b>Objective.</b> Besides the early detection and treatment of diabetic foot ulcers, being aware of the risk factors for major amputation plays a crucial role in preventing the major lower limb amputations. Major lower limb amputations are not just mentally and physically hard for patients, but also have an effect on patient's survival and are a financial burden on both patients and healthcare systems. <b>Subjects and Methods.</b> We defined 37 potential risk factors for major amputation and these risk factors were investigated among 507 patients who had ulcers in their feet and were seen by the diabetic foot ulcer council at Ege University Faculty of Medicine. In our study, 106 (20.9%) patients ended up undergoing major lower limb amputation. <b>Results.</b> The univariate analysis showed that 24 defined risk factors were statistically significant. In the multivariate analysis using the Cox regression model, 6 risk factors remained statistically significant. Multivariate-adjusted hazard ratios were 4.172 for hyperlipidemia, 3.747 for albumin <3.365 g/dL, 3.368 for C-reactive protein (CRP) >2.185 mg/L, 2.067 for presence of gangrenous Wagner stage, 1.931 for smoking tobacco >30 pack/year, and 1.790 for hematocrit (HCT) <31.5%. Most patients with major amputation presented with a neuroischemic foot (58%). Gender and age were not found to be risk factors for major amputation. Having less than 7% of hemoglobin A1c (HbA1c) levels had a direct proportion with major amputation numbers. The mortality rates in one year, two and three years after the major amputation operations were 24.6%, 30%, and 35.9%, respectively. <b>Conclusion.</b> Being familiar with these risk factors for major amputation is crucial for multi-disciplinary teams to take good care of patients with diabetic foot ulcers and to lower the need for major amputations.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"80-91"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9524223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pheochromocytoma associated with a succinate dehydrogenase subunit B mutation: A minireview and a case report.","authors":"Emad Rezkallah,&nbsp;Andrew Elsaify,&nbsp;Victorino Martin,&nbsp;Laura Viva,&nbsp;Sath Nag,&nbsp;Barnabas Green,&nbsp;Matthew Cheesman,&nbsp;Wael Elsaify","doi":"10.2478/enr-2023-0015","DOIUrl":"https://doi.org/10.2478/enr-2023-0015","url":null,"abstract":"<p><p><b>Objective.</b> Pheochromocytomas and paragangliomas are rare neuroendocrine tumors that arise from the chromaffin cells of the adrenal medulla or extra-adrenal tissues. These tumors are characterized by an excessive secretion of catecholamines, which are responsible for the clinical manifestation of the disease. Although most of these tumors are sporadic, underlying genetic abnormalities may be present in up to 24% of the cases. A succinate dehydrogenase subunit B (SDHB) mutation represents one of the rare presentations of the disease. In this study, we represent a rare case of pheochromocytoma associated with SDHB mutation. <b>Methods.</b> We performed a retrospective review of our case in addition to reviewing the available literature on the same topic. <b>Results.</b> A 17-year-old patient presented with sustained hypertension. Clinical, laboratory, and radiological evaluations confirmed the diagnosis of catecholamine-secreting tumor. Laparoscopic adrenalectomy was performed. Histopathological and genetic testing confirmed a pheochromocytoma associated with SDHB mutation. No recurrence was detected on two-years of follow up. <b>Conclusion.</b> Pheochromocytoma associated with SDHB mutation is a rare presentation. Genetic testing for suspecting cases is essential to help to establish the appropriate follow-up plan.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"121-127"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9598430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anemia types in hypothyroid patients in a Coimbatore tertiary care hospital: A prospective observational study.","authors":"Priyanka Sasidharan,&nbsp;Yoganathan Chidambaram,&nbsp;Bhargavi Kumar,&nbsp;Petchiappan Velammal,&nbsp;Sujith Kumar","doi":"10.2478/enr-2023-0014","DOIUrl":"https://doi.org/10.2478/enr-2023-0014","url":null,"abstract":"<p><p><b>Objective.</b> Hypothyroidism is a syndrome characterized by clinical manifestations associated with thyroid hormone deficiency. The thyroid hormone plays a pivotal role in the hematopoietic system and stimulates precursors of erythropoietin gene expression. Therefore, anemia is a common clinical manifestation in hypothyroid individuals. The goal of this study was to carry out a prospective analysis of the prevalence of anemia, its types, and the etiology behind the differing anemia morphology among hypothyroid patients. <b>Methods.</b> The study was conducted with a sample size of 100 patients suffering from hypothyroidism. The methodology of the study included a questionnaire and consent filling for general information followed by a complete blood test for assessment of blood count, peripheral smear, FT3/FT4 (free triiodothyronine/thyroxine), anemia profile, vitamin B12, folate, LDH (lactate dehydrogenase), reticulocyte count, and thyroid stimulating hormone (TSH). <b>Results.</b> The results of the study are in line with the previous studies and showed severe anemia and prevalence among women of reproductive age. Microcyte hypochromic anemia was found to be the most common type of morphological anemia, which was validated with low hemoglobin (Hb) levels, vitamin B12, FT3, and FT4. Additionally, TSH showed a positive correlation with reticulocyte count, LDH, and Hb in Pearson's correlation test. <b>Conclusion.</b> The study summarizes the need to investigate the underlying etiological agent responsible for better therapy and management of hypothyroidism and anemia suggesting also the use of oral iron supplements along with levothyroxine therapy.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"114-120"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9598884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of antipsychotics, haloperidol and olanzapine, on the expression of apoptosis-related genes in mouse mHippoE-2 cells and rat hippocampus.","authors":"Jana Osacka,&nbsp;Alexander Kiss,&nbsp;Zuzana Bacova,&nbsp;Andrej Tillinger","doi":"10.2478/enr-2023-0019","DOIUrl":"https://doi.org/10.2478/enr-2023-0019","url":null,"abstract":"<p><p><b>Objective.</b> Modified levels of pro- (caspase3, Bax) and anti-apoptotic (Bcl-2) regulatory proteins have been detected in certain brain areas of schizophrenic patients indicating a possible dysregulation of apoptosis. In the present study, effects of antipsychotics, haloperidol (HAL) and olanzapine (OLA), on the gene expression of caspase3 (<i>casp3</i>), <i>Bax</i> and <i>Bcl-2</i> were studied <i>in vitro</i> in mouse hippocampal mHippoE-2 cell line and <i>in vivo</i> in the hippocampus of MK-801 animal schizophrenia model with the aim to provide evidence that antipsychotics may affect the activity of apoptosis-related markers. <b>Methods.</b> mHippoE-2 cells were incubated with MK-801 (20 µM), HAL (10 µM), and OLA (10 µM) alone or combined, MK-801+HAL/OLA, for 24, 48, and 72 h. Male Sprague Dawley rats were injected with saline or MK-801 (0.5 mg/kg) for 6 days and since the 7th day, they were treated with vehicle (VEH), HAL (1 mg/kg) or OLA (2 mg/kg) for the next 7 days. The <i>casp3</i>, <i>Bax</i> and <i>Bcl-2</i> gene expression in mHippoE-2 cells and rat hippocampus was measured by RT-PCR. <b>Results.</b> In mHippoE-2 cells, <i>casp3</i> gene expression was increased by MK-801 and OLA treatments alone for 48 h, HAL treatment alone for 24 and 72 h, and co-treatment with MK-801+OLA for 24 and 72 h compared to controls. HAL and OLA suppressed the stimulatory effect of MK-801 on <i>casp3</i> mRNA levels in cells after 48 h of incubation. <i>Bax</i> mRNA levels in mHippoE-2 cells were decreased after HAL treatment for 24 and 48 h, and also after co-treatment with MK-801+HAL for 72 h. <i>In vivo</i>, MK-801 decreased mRNA levels of both pro-apoptotic markers, <i>casp3</i> and <i>Bax</i>, in hippocampus of VEH-treated rats and <i>Bax</i> mRNA levels in hippocampus of HAL-treated animals. OLA reversed the inhibitory effect of MK-801 on <i>casp3</i> expression in the VEH-treated animals. Neither MK-801 nor antipsychotics induced changes in the gene expression of anti-apoptotic marker <i>Bcl-2</i> in mHippoE-2 cells as well as hippocampus of rats. <b>Conclusions.</b> The results of the present study demonstrate that antipsychotics, HAL and OLA, may affect mRNA levels of pro-apoptotic markers in hippocampal cells <i>in vitro</i>, but not <i>in vivo</i>. The obtained data do not clearly support the assumed potentiating role of MK-801 in inducing apoptosis in specific brain areas and a possible protective role of antipsychotics against induction of apoptosis. The obtained data may contribute to a deeper insight into the neurodevelopmental changes connected with schizophrenia.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"152-161"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10250754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IGF-1 and IGFBP3 as indirect markers of hepatic insulin resistance and their relation to metabolic syndrome parameters in liver steatosis patients.","authors":"Emil Fraenkel,&nbsp;Ivica Lazurova","doi":"10.2478/enr-2023-0009","DOIUrl":"https://doi.org/10.2478/enr-2023-0009","url":null,"abstract":"<p><p><b>Objective.</b> The aim of the present study was to assess insulin-like growth factor 1 (IGF-1) and IGF-binding protein 3 (IGFBP3) as markers of insulin resistance in patients with prediabetes and type 2 diabetes mellitus (TDM2). <b>Patients and Methods.</b> This observational clinical study included 76 obese/overweight patients at the age of 45-75 years with T2DM on oral diabetic medication and ultrasonographically or by a computerized tomography (CT) diagnosed liver steatosis. Correlation analysis was performed between plasma levels of insulin, C-peptide, IGF-1, IGFBP3 and HOMA indexes on the one hand and between plasma levels of ALT, AST, triglyceride, cholesterol, and HDL cholesterol and body mass index (BMI) of patients on the other hand. In case of significant partial correlation coefficients, a multiple linear regression model with IGF-1 and IGFBP3 used as outcome variables adjusted for age and sex groups was calculated. According to these regression models, ROC curves were prepared with HOMA index=3 used as a classificator of insulin resistance. <b>Results.</b> Significant correlation was found between C-peptide and IGF-1 (r=0.24, p≤0.05), C-peptide and IGFBP3 (r=0.24, p≤0.05), IGFBP3 and cholesterol (r=0.22, p≤0.05) IGFBP3 and ALT (r=0.19, p≤0.05), HOMA index and triglycerides (r=0.22, p≤0.05), and HOMA index and ALT (r=0.23, p≤0.05). Significant correlation adjusted for age and gender was found between C-peptide and IGF-1 plasma levels (R2=0.20, p<0.05) with AUROC 0.685 (p≤0.01) and C-peptide and IGFBP3 plasma levels (R2=0.28, p<0.05) with AUROC 0.684 (p≤0.01). Significant correlation adjusted for age and gender was found between triglyceride and IGFBP3 plasma levels (R2=0.28, p<0.05) with AUROC 0.616 (p≤0.01). After the distribution of patients according to their IGFBP3 levels, we found a difference between the 1st and the 4th quartiles in terms of triglyceride levels. <b>Conclusion.</b> Our results demonstrate a fundamental role of IGF-1 and IGFBP3 in the patho-physiology of hepatic insulin resistance and suggest them as indirect indicators of the hepatic insulin resistance.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"69-79"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9524225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of DNA methylation change in TCF7L2 gene in the blood of type 2 diabetes mellitus as a predictive biomarker in Iraq Kurdistan region by using methylation-specific PCR.","authors":"Harem Othman Smail,&nbsp;Dlnya Asaad Mohamad","doi":"10.2478/enr-2023-0007","DOIUrl":"https://doi.org/10.2478/enr-2023-0007","url":null,"abstract":"<p><p><b>Objective.</b> Nowadays, type 2 diabetes mellitus (T2D) is the most common chronic endocrine disorder affecting an estimated 5-10% of adults worldwide, and this disease also rapidly increased among the population in the Kurdistan region. This research aims to identify DNA methylation change in the TCF7L2 gene as a possible predictive T2D biomarker. <b>Methods.</b> One hundred and thirteen participants were divided into three groups: diabetic (47), prediabetic (36), and control (30). The study was carried out in patients who visited the private clinical sector between August and December 2021 in Koya city (Iraq Kurdistan region) to determine DNA methylation status using a methylation-specific PCR (MSP) with paired primers for each methylated and non-methylated region. In addition, the X2 Kruskal-Wallis statistical and Wilcoxon signed-rank tests were used, p<0.05 was considered significant. <b>Results.</b> The results showed hypermethylation of DNA in the promoter region in diabetic and prediabetic groups compared to the healthy controls. Different factors affected the DNA methylation level, including body max index, alcohol consumption, family history, and physical activity with the positive Coronavirus. <b>Conclusion.</b> The results obtained indicate that DNA methylation changes in the TCF7L2 promoter region may be used as a potential predictive biomarker of the T2D diagnosis. However, the findings obtained in this study should be supported by additional data.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"53-60"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9544514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pituitary imaging findings in pediatric patients with idiopathic hypogonadotropic hypogonadism.","authors":"Eda Celebi Bitkin,&nbsp;Nursen Toprak,&nbsp;Serap Karaman","doi":"10.2478/enr-2023-0006","DOIUrl":"https://doi.org/10.2478/enr-2023-0006","url":null,"abstract":"<p><p><b>Objective.</b> Idiopathic hypogonadotropic hypogonadism in children is a disease leading to a puberty absence. Some hypothalamic and pituitary defects cause hypogonadotropic hypogonadism. Pituitary magnetic resonance imaging is routinely performed in these patients. In our study, we provide an information about pituitary pathologies associated with an idiopathic hypogonado-tropic hypogonadism in childhood. <b>Methods.</b> Twenty-two patients, who were admitted to the pediatric endocrine outpatient clinic of our hospital because of their undeveloped secondary sex characteristics during adolescence, were included in our study. Age, gender, history, physical examination findings, and laboratory tests were recorded in patients. Pituitary magnetic resonance imaging results were examined. The criteria for the diagnosis of hypogonadism were: absence of puberty or delayed puberty, clinical signs or symptoms of hypogonadism, and presence of low or normal gonadotropin levels. <b>Results.</b> In the present study, 22 patients were diagnosed with hypogonadotropic hypogonadism. The mean age of the patients was 15.90±1.09 years. Basal and stimulated luteinizing hormone and follicular stimulating hormone levels of the patients were found to be low. Prolactin, cortisol, adrenocorticotropic hormone, free thyroxine, and thyroid stimulating hormone levels were within normal limits in all patients. The pituitary magnetic resonance imaging revealed six patients with pituitary adenoma, one with empty sella turcica, and five with pituitary hypoplasia. <b>Conclusions.</b> The present data showed that in the presence of hypogonadotropic hypogonadism, the hypothalamic-pituitary abnormalities are more likely to be present in the children compared to the adult population. Thus, it can be strongly emphasized the importance of the pituitary imaging examination, especially in the idiopathic hypogonadotropic hypogonadism cases.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"48-52"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9179904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between preoperative calcium and parathormone levels with parathyroid gland volume.","authors":"Emad Rezkallah,&nbsp;Andrew Elsaify,&nbsp;Ragai Hanna,&nbsp;Wael Elsaify","doi":"10.2478/enr-2023-0002","DOIUrl":"https://doi.org/10.2478/enr-2023-0002","url":null,"abstract":"<p><p><b>Objective.</b> Hyperparathyroidism is a prevalent disease with parathyroid adenomas being the most common cause. Surgical excision remains the standard treatment for parathyroid adenoma. Successful preoperative localization of the parathyroid adenoma could facilitate the decision regarding the extent of surgical exploration. The aim of the current study was to assess the correlation between the preoperative values of parathyroid hormone and ionized calcium with the adenoma weight and volume in patient with primary hyperparathyroidism caused by single-gland adenoma. <b>Patients and Methods.</b> We did this retrospective review for all patients who were diagnosed with primary hyperparathyroidism due to a solitary parathyroid adenoma in our general surgery department over 4 years. SPSS software was used to get the correlation coefficient between the peak preoperative levels of calcium and parathyroid hormone with the parathyroid adenoma weight and volume. <b>Results.</b> Ninety-nine patients were included into the study. The average age at surgery was 62.65±12.00 years. The correlation coefficient between the adenoma volume and weight with the preoperative ionized calcium level was weakly positive (r=0.329, p<0.01) and (r=0.281, p=0.019), respectively, while the correlation with the preoperative parathyroid hormone level was stronger (r=0.708, p<0.01) and (r=0.650, p<0.01), respectively. <b>Conclusions.</b> The strong positive relationship between the preoperative parathyroid hormone and calcium levels with the parathyroid adenoma size and weight can help the surgeon to predict the volume of the involved gland and avoid an unnecessary dissection.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"12-17"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9179905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ERN1 dependent impact of glucose and glutamine deprivations on PBX3, PBXIP1, PAX6, MEIS1, and MEIS2 genes expression in U87 glioma cells.","authors":"Dariia O Krasnytska,&nbsp;Yuliia M Viletska,&nbsp;Dmytro O Minchenko,&nbsp;Olena O Khita,&nbsp;Dariia O Tsymbal,&nbsp;Anastasiia A Cherednychenko,&nbsp;Halyna E Kozynkevych,&nbsp;Nataliia S Oksiom,&nbsp;Oleksandr H Minchenko","doi":"10.2478/enr-2023-0005","DOIUrl":"https://doi.org/10.2478/enr-2023-0005","url":null,"abstract":"<p><p><b>Objective.</b> Homeobox genes play a fundamental role in the embryogenesis, but some of them have been linked to oncogenesis. The present study is aimed to investigate the impact of glucose and glutamine deprivations on the expression of homeobox genes such as <i>PAX6</i> (paired box 6), <i>PBX3</i> (<i>PBX</i> homeobox 3), <i>PBXIP1</i> (PBX homeobox interacting protein 1), <i>MEIS1</i> (<i>MEIS</i> homeobox 1), and <i>MEIS2</i> in ERN1 knockdown U87 glioma cells with the intent to reveal the role of ERN1 (endoplasmic reticulum to nucleus signaling 1) signaling pathway on the endoplasmic reticulum stress dependent regulation of homeobox genes. <b>Methods.</b> The control (transfected by empty vector) and ERN1 knockdown (transfected by dominant-negative ERN1) U87 glioma cells were exposed to glucose and glutamine deprivations for 24 h. The cells RNA was extracted and reverse transcribed. The expression level of <i>PAX6</i>, <i>PBX3</i>, <i>PBXIP1</i>, <i>MEIS1</i>, and <i>MEIS2</i> genes was evaluated by a real-time quantitative polymerase chain reaction analysis and normalized to ACTB. <b>Results.</b> It was found that glucose deprivation down-regulated the expression level of <i>PAX6</i>, <i>MEIS1</i>, and <i>MEIS2</i> genes in control glioma cells, but did not significantly alter <i>PBX3</i> and <i>PBXIP1</i> genes expression. At the same time, ERN1 knockdown significantly modified the sensitivity of all studied genes to glucose deprivation. Other changes in gene expression were detected in control glioma cells under the glutamine deprivation. The expression of <i>PBX3</i> and <i>MEIS2</i> genes was down- while <i>PAX6</i> and <i>PBXIP1</i> genes up-regulated. Furthermore, ERN1 knockdown significantly modified the effect of glutamine deprivation on the majority of studied genes expression in U87 glioma cells. <b>Conclusion.</b> The results of the present study demonstrate that the exposure of U87 glioma cells under glucose and glutamine deprivations affected the expression of the majority of the studied homeobox genes and that the sensitivity of <i>PAX6</i>, <i>PBX3</i>, <i>PBXIP1</i>, <i>MEIS1</i>, and <i>MEIS2</i> genes expression under these experimental conditions is mediated by ERN1, the major pathway of the endoplasmic reticulum stress signaling.</p>","PeriodicalId":11650,"journal":{"name":"Endocrine regulations","volume":"57 1","pages":"37-47"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9179906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}