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Nitric Oxide and Renal Regulation of Sodium Excretion 一氧化氮和肾脏对钠排泄的调节
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024684
F. Salazar
{"title":"Nitric Oxide and Renal Regulation of Sodium Excretion","authors":"F. Salazar","doi":"10.3109/10623329609024684","DOIUrl":"https://doi.org/10.3109/10623329609024684","url":null,"abstract":"The importance of nitric oxide in the acute and long-term regulation of arterial pressure and renal function is supported by the results obtained in many studies. Reports described in this brief review provide evidences that nitric oxide plays an important role in regulating the urinary sodium and water excretion in basal conditions and during increments in arterial pressure and extracellular volume. Data showing an important interaction between nitric oxide and angiotensin II or prostaglandins in the regulation of the renal excretory function is also provided. These studies support the concept that the development of sodium sensitive hypertension could be secondary to a deficient nitric oxide production.","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"57 1","pages":"77-83"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74630401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adhesion and Growth of Brain Microvascular Endothelial Cells on Treated Glass 脑微血管内皮细胞在处理玻璃上的粘附和生长
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024705
M. Nobles, N. Abbott
{"title":"Adhesion and Growth of Brain Microvascular Endothelial Cells on Treated Glass","authors":"M. Nobles, N. Abbott","doi":"10.3109/10623329609024705","DOIUrl":"https://doi.org/10.3109/10623329609024705","url":null,"abstract":"For most studies of cultured endothelium, cells are grown on tissue culture plastic. However, for microscopic studies involving the use of fluorescent dyes, the autofluorescence of the plastic can be a problem. Glass is a good alternative to plastic in this case, but standard methods for coating tissue culture plastic to provide an adhesive substrate do not work well on glass. We here report studies on the morphology and growth characteristics of rat brain microvascular endothelial cells growing on glass treated to improve adhesion by changing the collagen-to-glass binding properties and finally coated with collagen. Three methods were tested, using primary cultures of rat brain endothelial cells, and an immortalized rat brain endothelial cell line (RBE4). Cells grew poorly on an extracellular matrix laid down by prior culture of rat type I astrocytes. Cells grew equally well on glass coated with poly-L-lysine or its succinylated derivative. However, a further method gave an even more stable and confluent...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"28 1","pages":"297-307"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74804885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Human umbilical vein endothelial cells express P450 2C8 mRNA : cloning of endothelial P450 epoxygenase 人脐静脉内皮细胞表达P450 2C8 mRNA:内皮P450环氧化酶的克隆
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024698
Jane H.-C. Lin, Y. Kobari, Yi Zhu, M. Stemerman, K. Pritchard
{"title":"Human umbilical vein endothelial cells express P450 2C8 mRNA : cloning of endothelial P450 epoxygenase","authors":"Jane H.-C. Lin, Y. Kobari, Yi Zhu, M. Stemerman, K. Pritchard","doi":"10.3109/10623329609024698","DOIUrl":"https://doi.org/10.3109/10623329609024698","url":null,"abstract":"Human umbilical vein endothelial cells (EC) metabolize arachidonic acid (AA) through three major pathways—cyclooxygenase, lipoxygenase and cytochrome P450 (P450) isozymes. Previously, we have shown that pathophysiological concentrations of native low density lipoprotein (n-LDL) increase EC P450-dependent epoxyeicosatrienoic acid (EET) production. The present study was designed to identify putative P450 isozymes involved in EC epoxidation of AA. Reverse transcription—polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) were employed to detect P450 2 family cDNA from EC mRNA. Degenerate primers complimenting 2 homologous regions from 6 different P450 2 families were designed to capture a 440 bp cDNA fragment corresponding to the heme binding region of P450 2 isozymes. RT-PCR of EC total RNA with these primers amplified a 440 bp fragment. After gel purification, the fragment was cloned, sequenced and found to share a high degree of identity with human liver P450 2C8 and 2C9. New pri...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"29 1","pages":"219-229"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82158635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 41
Direct Measurement of Nitric Oxide Release from Cultured Endothelial Cells Stimulated by Bradykinin or Ramiprilat 缓激肽或雷米普利特刺激内皮细胞一氧化氮释放的直接测量
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024688
G. Wiemer, B. Pierchala, Š. Mesároš, B. Scholkens, T. Malinski
{"title":"Direct Measurement of Nitric Oxide Release from Cultured Endothelial Cells Stimulated by Bradykinin or Ramiprilat","authors":"G. Wiemer, B. Pierchala, Š. Mesároš, B. Scholkens, T. Malinski","doi":"10.3109/10623329609024688","DOIUrl":"https://doi.org/10.3109/10623329609024688","url":null,"abstract":"Stimulation of endothelial nitric oxide production by bradykinin or the angiotensin converting enzyme inhibitor “ramiprilat” has been indirectly assessed in terms of intracellular cyclic GMP accumulation. However, direct measurement of endothelial nitric oxide synthesis and release has not been analyzed. Using a selective porphyrinic microsensor, nitric oxide release was detected from single primary cultured bovine aortic endothelial cells. Maximal nitric oxide release of 540 ± 38 or 122 ± 10 n mol/L was achieved by bradykinin at 5 × 10−8 mol/L or by ramiprilat at 10−7 mol/L respectively. The time course of nitric oxide release by bradykinin showed a rapid initial production rate (22.5 ± 1.6 nmol/L/sec, 4.2% increase per second) and was transient within 15 min (decay rate 0.60 ± 0.05 nmol/L/sec). In contrast, the initial production rate of nitric oxide release by ramiprilat was slow (0.34 ± 0.03 nmol/L/sec, 0.28% increase per second) and reached a plateau level after 6 min, which remained stable for at le...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"258 1","pages":"119-125"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77092213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
Release of GM-CSF, sE-Selectin, and SICAM-1 by Human Vascular Endothelium Stimulated with Gram-Negative and Gram-Positive Bacterial Components 革兰氏阴性和革兰氏阳性细菌成分刺激人血管内皮释放GM-CSF、se -选择素和SICAM-1
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024677
G. Donnarumma, F. Brancaccio, Gabriella Cipollaro Del'ero, A. Folgore, A. Marcatili, M. Galdiero
{"title":"Release of GM-CSF, sE-Selectin, and SICAM-1 by Human Vascular Endothelium Stimulated with Gram-Negative and Gram-Positive Bacterial Components","authors":"G. Donnarumma, F. Brancaccio, Gabriella Cipollaro Del'ero, A. Folgore, A. Marcatili, M. Galdiero","doi":"10.3109/10623329609024677","DOIUrl":"https://doi.org/10.3109/10623329609024677","url":null,"abstract":"Gram-negative (Salmonella typhimurium porins and lipopolysaccharide-R), and Gram-positive bacterial components (lipoteichoic acid, muramic acid, muramyl-dipeptide, adjuvant peptide, protein A, toxic shock syndrome toxin-1, α-hemolysin) were tested for their ability to stimulate both the release of Granulocyte-Monocyte Colony Stimulating Factor (GM-CSF), soluble Intercellular Adhesion Molecule-1 (ICAM-1) and soluble E-selectin from human endothelial cells, and the surface expression of the adhesion molecules. Salmonella typhimurium porins and lipopolysaccharide-R (LPS-R) were able to induce the release of GM-CSF, sE-selectin and sICAM-1 in a dose dependent fashion. The greatest release of these factors was obtained using pork at a concentration of 5μg/ml and LPS-R at a concentration of 1μg/ml. The kinetics of release showed that LPS-R was able to stimulate the production of these factors earlier than porins but at a lower rate. Porins and LPS-R was also able to up-regulate the surface expression of E-selec...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"294 1","pages":"11-22"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90801098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Modulation of Substance P-Induced K+ Current in Coronary Endothelium p物质诱导的冠状动脉内皮K+电流的调节
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024695
N. R. Sharma, M. Davis
{"title":"Modulation of Substance P-Induced K+ Current in Coronary Endothelium","authors":"N. R. Sharma, M. Davis","doi":"10.3109/10623329609024695","DOIUrl":"https://doi.org/10.3109/10623329609024695","url":null,"abstract":"Substance P (SP) activates an outward K+ current, membrane hyperpolarization and increases cytosolic Ca2+ ([Ca2+],) in porcine coronary artery endothelial cells (PCAECs). We tested the importance of Ca2+, CAMP, cGMP, arachidonic acid and G protein-linked pathways in modulating whole-cell K+ currents activated by SP. Current or membrane potential and [Ca2+], were measured simultaneously in single cells in response to SP (10 nM), using the perforated patch technique and fura-2 microfluorimetry. Partial block of Kcachannels by D-tubocurarine resulted in a significant reduction in SP-induced membrane hyperpolarization and the plateau phase of the cell [Ca2+]i response. In PCAECs preloaded with BAPTA, SP failed to elevate [Ca2+]i or activate outward current. In Ca2+-free bath solution, SP induced transient elevations in [Ca2+]i and outward current. With SP still present, addition of 1 mM Ca2+ to the bath after [Ca2+]i returned to resting levels elevated [Ca2+]i and elicited outward current. Acute application o...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"29 1","pages":"189-197"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86105042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Specificity of C-type and atrial natriuretic peptides in human blood vessels c型和心房利钠肽在人血管中的特异性
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024678
Chi-Ming Wei, V. Miller, H. Schaff, J. Burnett
{"title":"Specificity of C-type and atrial natriuretic peptides in human blood vessels","authors":"Chi-Ming Wei, V. Miller, H. Schaff, J. Burnett","doi":"10.3109/10623329609024678","DOIUrl":"https://doi.org/10.3109/10623329609024678","url":null,"abstract":"We investigated the actions of the two structurally similar but genetically distinct endothelium-derived vasoactive natriuretic peptides, C-type natriuretic peptide (CNP) and atrial natriuretic peptide (ANP), upon isolated human internal mammary arteries and saphenous veins. Based upon previous studies in canine arteries and veins, we hypothesized that CNP would selectively cause relaxation of human veins while ANP would be selective for human arteries. Rings with and without endothelium of human internal mammary arteries and saphenous veins from patients under going coronary artery bypass grafting were suspended for measurement of isometric force in an organ chamber. CNP caused significant concentration-dependent relaxations in saphenous veins with and without endothelium contracted with phenylephrine (10−6 M). In marked contrast, ANP caused no relaxation in saphenous veins either with or without endothelium. In rings of internal mammary arteries, ANP caused significant concentration-dependent relaxation...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"25 1","pages":"23-28"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78577272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Endothelin Modulation of Pumping Activity in Bovine Mesenteric Lymphatic Vessels 内皮素对牛肠系膜淋巴管泵送活性的调节作用
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024691
Zheng Yuan, D. Atchison, M. Johnston
{"title":"Endothelin Modulation of Pumping Activity in Bovine Mesenteric Lymphatic Vessels","authors":"Zheng Yuan, D. Atchison, M. Johnston","doi":"10.3109/10623329609024691","DOIUrl":"https://doi.org/10.3109/10623329609024691","url":null,"abstract":"We studied the effects of endothelin family peptides on the pumping responses of lymphatic vessels, the receptors involved and whether vessel-derived endothelin played a role in the myogenic response to changes in transmural pressure. Postnodal bovine mesenteric lymphatics were suspended in an organ bath preparation with both inflow and outflow ends cannulated. Input to the ducts was provided from a reservoir filled with Krebs solution. With a 6 cm H2O fixed transmural pressure applied to the vessels to initiate spontaneous contractions, ET-1, ET-2 and ET-3 (tested between 10−10 and 10−8PM) depressed lymphatic pumping in a concentration-dependent fashion with the order of potency being ET-1 = ET-2 > ET-3. The concentrations of ET-1 and ET-2 that depressed pumping 50% were 0.96 × 10−9M and 0.95 × 10−9M respectively. Similarly, when transmural pressures were varied in 2 cm H2O increments between 0 and 14 cm H2O, ET-1 at 10−9 and 10−8M significantly depressed fluid pumping at the majority of distending press...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"28 1","pages":"151-158"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82872884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Hypertensive Serum Enhances Human Vascular Cell Growth and Modulates Endothelial Angiotensin II and Endothelin in Culture 高血压血清促进人血管细胞生长和调节内皮血管紧张素II和内皮素
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024693
Rosella Sbarbati-Delguerra, E. Fommei, F. Pecori, P. Gazzetti, M. G. Chicca
{"title":"Hypertensive Serum Enhances Human Vascular Cell Growth and Modulates Endothelial Angiotensin II and Endothelin in Culture","authors":"Rosella Sbarbati-Delguerra, E. Fommei, F. Pecori, P. Gazzetti, M. G. Chicca","doi":"10.3109/10623329609024693","DOIUrl":"https://doi.org/10.3109/10623329609024693","url":null,"abstract":"We studied the influence of hypertensive (HT) serum on human umbilical vein endothelial cell (HUVEC) and on human umbilical artery smooth muscle cell (HUASMC) proliferation. We also tested the possible influence of the HT serum on vasoconstrictor substances released by HUVECs.Pools of HT and normotensive (NT) sera were collected and used to supplement cell culture medium. Both HUVECs and HUASMCs were conditioned with culture media supplemented with either HT or NT sera. After conditioning, both types of cells were tested for proliferative capacity. Under the same experimental conditions the HUVEC conditioned medium was assayed for angiotensin II (AII) and endothelin 1 (ET,). All and ET, were also assayed after acute exposure to HT serum of HUVEC preconditioned with NT serum.Our results showed that HUVEC and HUASMC conditioned with HT serum substantially enhanced cell proliferation. Preconfluent HUVECs conditioned with HT serum showed higher levels of AZZ and lower levels of ET1 compared with the controls ...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"47 1","pages":"171-176"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89178812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Neonatal Sensory Denenration Affects the Expression of Endothelial Peptides in the Adult Rat Pulmonary Artery: More Cells Contain Substance P and Less Contain Endothelin 新生儿感觉变性影响成年大鼠肺动脉内皮肽的表达:P物质含量增加，内皮素含量减少
Endothelium-journal of Endothelial Cell Research Pub Date : 1996-01-01 DOI: 10.3109/10623329609024683
P. Milner, A. Loesch, G. Burnstock
{"title":"Neonatal Sensory Denenration Affects the Expression of Endothelial Peptides in the Adult Rat Pulmonary Artery: More Cells Contain Substance P and Less Contain Endothelin","authors":"P. Milner, A. Loesch, G. Burnstock","doi":"10.3109/10623329609024683","DOIUrl":"https://doi.org/10.3109/10623329609024683","url":null,"abstract":"There is evidence that long-term changes in the innervation of vascular smooth muscle alters the flow-stimulated release of vasoactive substances from the endothelium. To investigate the endothelial content of these substances following chronic sensory denervation, the distribution of endotheli-1 (ET-l), substance P (SP) and arginine vasopressin (AVP) in mature rat pulmonary artery endothelial cells was examined after neonatal treatment with the selective sensory neurotoxin, capsaicin. After immunolabelling with antibodies to the vasoactive peptides, Hautchen preparations of sheets of endothelial cells were observed and the percentage of immunopositive cells recorded. Preparations from capsaicin-treated rats displayed significantly more cells immunopositive to SP than controls (36% ± 3, n = 4 versus 9% ± 4, n = 4, P < 0.01) and less cells immunopositive to ET-1 (36% ± 4, n = 4 versus 56% ± 3, n = 4, P < 0.01). The distribution of vasopressin-immunopositive cells was unchanged by capsaicin treatment (10% ±...","PeriodicalId":11588,"journal":{"name":"Endothelium-journal of Endothelial Cell Research","volume":"63 1","pages":"71-76"},"PeriodicalIF":0.0,"publicationDate":"1996-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89124944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
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