Eicosanoids最新文献_第6页

Prolactin secretion in anterior pituitary cells: effect of eicosanoids. 垂体前叶细胞催乳素分泌:类二十烷酸的作用。

Eicosanoids Pub Date : 1992-01-01

J R Cashman, K W Snowdowne

{"title":"Prolactin secretion in anterior pituitary cells: effect of eicosanoids.","authors":"J R Cashman, K W Snowdowne","doi":"","DOIUrl":"","url":null,"abstract":"Ovariectomized Fischer 344 rats were implanted with silastic capsules containing estradiol for approximately 4 weeks to increase the number of lactotrophs in the anterior pituitary gland. Anterior pituitary cells were enzymatically dispersed and cultured for 1 day and challenged with eicosanoids or other prolactin (PRL) secretagogues. The isolated pituitary cells from estradiol-pretreated animals exhibited an increase in the ability to secrete PRL in the presence of maximally effective concentrations of thyrotropin releasing hormone (TRH), arachidonic acid or 5,6-epoxyeicosatrienoic acid (5,6-EET). Anterior pituitary cells from estradiol-pretreated animals also showed an increased activity of cytochrome P-450. The possible involvement of cytochrome P-450 in PRL secretion from anterior pituitary animal cells isolated from animals pretreated with estradiol was shown by the fact that these anterior pituitary cells increased the synthesis of 5,6-EET, a potent PRL releasing agent. TRH and 5,6-EET increased the mobilization of both cyclic AMP and cytoplasmic calcium to about the same extent. The data suggest that cytochrome P-450 is important in the release of PRL from anterior pituitary cells isolated from estradiol-pretreated Fischer 344 rats and that a product of cytochrome P-450-catalyzed epoxidation of arachidonic acid, 5,6-EET, plays a significant role in the release of PRL.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 3-4","pages":"153-61"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12511498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of bioanalytical determinations of Iloprost, a chemically stable PGI2 mimetic, by conventional radioimmunoassay (RIA) and scintillation proximity assay (SPA). 传统放射免疫法(RIA)和闪烁接近法(SPA)测定化学稳定的PGI2模拟物伊洛前列素的比较。

Eicosanoids Pub Date : 1992-01-01

M Hildebrand, T Louton, A Schütt

{"title":"Comparison of bioanalytical determinations of Iloprost, a chemically stable PGI2 mimetic, by conventional radioimmunoassay (RIA) and scintillation proximity assay (SPA).","authors":"M Hildebrand, T Louton, A Schütt","doi":"","DOIUrl":"","url":null,"abstract":"The scintillation proximity assay is a novel variant of classical radioimmunoassay. It can be performed as a single tube measurement because the separation of bound and unbound tracer fraction is avoided. In principle, microbeads are coated with anti-species antibodies that can couple with the respective antiserum used for RIA. By means of special cores, light emission takes place if labelled, antiserum-bound tracer is coupled to the anti-species antibody on the fluomicrosphere surface. In the present report, the novel assay was compared to a validated RIA for the bioanalysis of the PGI2 mimetic, Iloprost. Extraction recovery of Iloprost was approximately 90% at pH less than or equal to 4. The detection limit of the novel assay was 2-4 pg/sample, corresponding to 10-20 pg/ml plasma (if 0.2 ml plasma was used). Coefficients of variations were 9, 7 and 6% (within-day, n = 5) and 30, 11 and 10% (day-to-day, n = 10) at 50, 100 and 200 pg/ml. RIA and SPA levels of Iloprost measured in human plasma samples (n = 428) were similar. The SPA method exhibits both a similar specificity and detection limit to RIA and will be used for further analyses.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 1","pages":"5-8"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12557422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prostaglandin photoaffinity probes: synthesis and binding affinity of aryl azide-substituted C-1 esters of prostaglandin F2 alpha. 前列腺素光亲和探针:前列腺素F2 α的芳基叠氮取代C-1酯的合成和结合亲和性。

Eicosanoids Pub Date : 1992-01-01

M Golinski, M Heine, D J Orlicky, T A Fitz, D S Watt

引用次数: 0

Annexins and phospholipase A2 inhibition. 膜联蛋白和磷脂酶A2抑制。

Eicosanoids Pub Date : 1992-01-01

W J Buhl

引用次数: 0

Diet-induced atherosclerosis in rabbits alters vascular prostacyclin release. 饮食诱导的动脉粥样硬化改变血管前列环素释放。

Eicosanoids Pub Date : 1992-01-01

J Brunkwall, E Mattsson, D Bergqvist

{"title":"Diet-induced atherosclerosis in rabbits alters vascular prostacyclin release.","authors":"J Brunkwall, E Mattsson, D Bergqvist","doi":"","DOIUrl":"","url":null,"abstract":"Atherosclerosis is complicated by thrombosis and it has been suggested that a decreased prostacyclin and/or an increased thromboxane release from the vascular wall could play a part in this process. There are few reports dealing with determinations of prostanoid release from physiologically perfused normal and atherosclerotic vessel walls or from perfused atherosclerotic hearts. Therefore, fourteen rabbits were given 2% cholesterol added to the diet for 26 weeks, which led to atherosclerosis, verified by scanning electron microscopy. Five animals died, and in the surviving nine, as well as from ten healthy rabbits, the aorta was excised. The vessels were perfused with pulsatile flow at physiologic pressure five times for fifteen minutes with the addition of arachidonic acid to the last perfusate. Prostacyclin and thromboxane were determined as their stable degradation products 6-keto-PGF1 alpha and TxB2 by radio-immuno assay. Atherosclerotic and normal animals had the same initial release of prostacyclin but in the atherosclerotic animals the release did not decline with time as it did in the normal animals. The response to arachidonic acid was also higher in the atherosclerotic group. The release of thromboxane was not altered in the atherosclerotic group compared to the control group. It is concluded that prostacyclin release from aortas is altered in rabbits with diet-induced atherosclerosis compared to normal rabbit aortas, but that vascular thromboxane production is not.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"5 3-4","pages":"197-202"},"PeriodicalIF":0.0,"publicationDate":"1992-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12467468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of prostanoids in pediatric diseases employing mass spectrometric techniques. 利用质谱技术研究前列腺素在儿科疾病中的作用。

Eicosanoids Pub Date : 1992-01-01

H W Seyberth, H Schweer, B Tönshoff, A Leonhardt

引用次数: 0

The role of leukotriene D4 in septic shock models. 白三烯D4在感染性休克模型中的作用。

Eicosanoids Pub Date : 1992-01-01

T Hartung, G Tiegs, A Wendel

引用次数: 0

The role of eicosanoids in reproduction. 类二十烷酸在生殖中的作用。

Eicosanoids Pub Date : 1992-01-01 DOI: 10.1002/9783527613625.CH8

H. P. Zahradnik, W. Schäfer, J. Neulen, B. Wetzka, T. Gaillard, J. Tielsch, F. Casper

引用次数: 8

LDL receptor-dependent polyunsaturated fatty acid transport and metabolism. LDL受体依赖性多不饱和脂肪酸的转运和代谢。

Eicosanoids Pub Date : 1992-01-01 DOI: 10.1007/978-3-642-84949-7_11

A. Habenicht, P. Salbach, U. Janssen‐Timmen

引用次数: 10

PGD2 and its mimetic ZK 110.841 are potent inhibitors of receptor-mediated activation of human neutrophils. PGD2及其类似物ZK 110.841是受体介导的人中性粒细胞活化的有效抑制剂。

Eicosanoids Pub Date : 1991-01-01

P Ney, K Schrör

{"title":"PGD2 and its mimetic ZK 110.841 are potent inhibitors of receptor-mediated activation of human neutrophils.","authors":"P Ney, K Schrör","doi":"","DOIUrl":"","url":null,"abstract":"The action of PGD2 and its mimetic ZK 110.841 ((5Z,13E)-(9R,11R,15S)-9-chloro-15-cyclohexyl-11,15- dihydroxy-16,17,18,19, 20-pentanor-5,13-prostadienoic acid) was compared to PGE1 in vitro on superoxide anion generation, degranulation, leukotriene (LT) B4 release and Ca++ fluxes in human polymorphonuclear leukocytes (PMN). All compounds were potent inhibitors of formyl-methionyl-leucyl-phenylalanine (FMLP)- and platelet-activating factor (PAF)-induced superoxide anion generation, beta-glucuronidase release and Ca++ influx. The PAF-induced release of LTB4 in the presence of 10 mumoles/l arachidonic acid was significantly attenuated by these prostaglandins. This inhibition of PMN function was paralleled by an increase in cellular cAMP levels. The molar potency of the prostaglandins used was comparable, although the D-type compounds appeared slightly more potent in some PMN function tests. None of the substances affected PMN activation induced by the calcium inophore calcimycin (A23187). The data demonstrate an effective inhibition of receptor-mediated (FMLP, PAF) PMN activation by PGD2 and its mimetic ZK 110.841, suggesting either an inhibitory PGD2 receptor on human PMN or action of PGD2 at the PGE receptor. PGD2 is a labile compound in vivo and is rapidly metabolized into a number of products with different biological properties. Since ZK 110.841 lacks this instability, this compound may serve as an important tool to classify PGD2-mediated reactions.","PeriodicalId":11520,"journal":{"name":"Eicosanoids","volume":"4 1","pages":"21-8"},"PeriodicalIF":0.0,"publicationDate":"1991-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12813653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0