Egyptian Journal of Basic and Applied Sciences最新文献_第4页

Molecular docking and insecticidal activity of Pyrus communis L. extracts against disease vector, Musca domestica L. (Diptera: muscidae) 黄柏提取物对病媒家蝇（双翅目：蝇科）的分子对接和杀虫活性

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-11-28 DOI: 10.1080/2314808X.2023.2283268

Ahmed Z. I. Shehata, Esraa A. Elhawary, Mostafa M. Mokhtar, Hassan O. Waheeb, Mohamed Fares, D. E. Emam, Nader A. Bakr, Ahmed M. Sadek, Mohamed A. M. El-Tabakh

{"title":"Molecular docking and insecticidal activity of Pyrus communis L. extracts against disease vector, Musca domestica L. (Diptera: muscidae)","authors":"Ahmed Z. I. Shehata, Esraa A. Elhawary, Mostafa M. Mokhtar, Hassan O. Waheeb, Mohamed Fares, D. E. Emam, Nader A. Bakr, Ahmed M. Sadek, Mohamed A. M. El-Tabakh","doi":"10.1080/2314808X.2023.2283268","DOIUrl":"https://doi.org/10.1080/2314808X.2023.2283268","url":null,"abstract":"ABSTRACT The common housefly (Musca domestica) is widely recognized as a significant pest and can transmit many pathogens. The habitual use of chemical insecticides against housefly has led to detrimental effects on humans, non-target species, and environment. Therefore, there is an urgent demand for new, sustainable alternatives of housefly control. This research investigates the effects of different extracts from common pear (Pyrus communis) leaves on M. domestica larvae, and lifecycle progression. Musca domestica was identified through DNA barcoding, and a sequernce for the COX1 gene was submitted to NCBI gene bank, with accession number of OQ363313. Results showed that extracts from P. communis leaves were lethal to the larvae at 650 and 150 ppm, respectively, with the n-hexane extract proving to be more efficacious. Also, tested extracts prolonged developmental stages of both larvae and pupae. Females emerged from treated larvae laid fewer eggs. A molecular docking was performed to examine the interactions between extract’s molecules and a crucial protein in M. domestica, Ls-AChBP (PDB ID: 2zju). Five compounds were identified as particularly effective: Tetratetracontane, Octacosyl acetate, Hexatriacontane, Eicosyl heptafluorobutyrate, and Bis (2-ethylhexyl) phthalate. The results indicate that these pear leaf extracts hold promise as alternative agents for managing M. domestica populations.","PeriodicalId":11512,"journal":{"name":"Egyptian Journal of Basic and Applied Sciences","volume":"19 1","pages":"800 - 811"},"PeriodicalIF":0.0,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139216674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does Dactyloctenium aegyptium ethanolic extract protect against hormonal, histological and immunohistochemical alterations induced by sodium fluoride in rat adrenal gland? Dactyloctenium aegyptium乙醇提取物能防止氟化钠诱导的大鼠肾上腺激素、组织学和免疫组化改变吗？

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-11-27 DOI: 10.1080/2314808X.2023.2281043

Shimaa N. El-Sayed, Abdelsamed I. Elshamy, A. H. Farrag, Rania A. Ahmed, Aida A. Hussein

{"title":"Does Dactyloctenium aegyptium ethanolic extract protect against hormonal, histological and immunohistochemical alterations induced by sodium fluoride in rat adrenal gland?","authors":"Shimaa N. El-Sayed, Abdelsamed I. Elshamy, A. H. Farrag, Rania A. Ahmed, Aida A. Hussein","doi":"10.1080/2314808X.2023.2281043","DOIUrl":"https://doi.org/10.1080/2314808X.2023.2281043","url":null,"abstract":"ABSTRACT This work aimed to perform a biochemical, histological, and immunohistochemical assessment of the influence of sodium fluoride on the adrenal gland of rats and the possible ameliorating role of Dactyloctenium aegyptium (D. aegyptium). Thirty-five male albino rats were divided into five equal groups: group I served as the control group, group II received sodium fluoride (5 mg/kg), group III received D. aegyptium (200 mg/kg), and groups IV and V received sodium fluoride and D. aegyptium (100 and 200 mg/kg respectively) simultaneously for 28 days. Aldosterone, corticosterone, nitric oxide, sodium, potassium, and chloride were measured in serum samples. Adrenal gland specimens were processed for histological, histochemical, and immunohistochemical studies. The sodium fluoride group recorded a significant reduction in serum levels of aldosterone, corticosterone, potassium, and chloride (p ≤ 0.0001) and a significant increase in nitric oxide (p ≤ 0.0001) and serum sodium (p = 0.0076) when compared with the control group. Histological changes in the sodium fluoride treated group showed cytoplasmic vacuolation, scattered apoptotic cells, and hemorrhage. The sodium fluoride treatment significantly decreased glycogen (p = 0.0002), total protein) p = 0.0007), and DNA (p ≤ 0.0001) and increased inducible nitric oxide synthase (p = 0.0001). Co-treated groups with D. aegyptium showed almost average values for most parameters and a nearly standard architecture. The present data answers the question of our research that D. aegyptium ethanolic extract could protect against sodium fluoride’s adverse effects in rat adrenal glands on the physiological and histological levels.","PeriodicalId":11512,"journal":{"name":"Egyptian Journal of Basic and Applied Sciences","volume":"5 1","pages":"784 - 799"},"PeriodicalIF":0.0,"publicationDate":"2023-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139232016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does Lactobacillus plantarum- ATCC8014 alleviate inflammation? In vitro and in vivo appraisal 植物乳杆菌ATCC8014是否能缓解炎症?体外和体内评价

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-11-03 DOI: 10.1080/2314808x.2023.2272389

Manal A. El-Shal, Samia Haroun, Nanis Gamal Allam, Reda Shehata, Mohammed Yosri, Mahmoud M. Elaasser, Gamal Abdel-Fattah

{"title":"Does Lactobacillus plantarum- ATCC8014 alleviate inflammation? In vitro and in vivo appraisal","authors":"Manal A. El-Shal, Samia Haroun, Nanis Gamal Allam, Reda Shehata, Mohammed Yosri, Mahmoud M. Elaasser, Gamal Abdel-Fattah","doi":"10.1080/2314808x.2023.2272389","DOIUrl":"https://doi.org/10.1080/2314808x.2023.2272389","url":null,"abstract":"Probiotics are beneficial for certain illnesses, and a variety of clinical studies have reported remarkable effects in the cutaneous system. The objective of current study was to test the antimicrobial impact of Lactobacillus plantarum-ATCC8014 filtrate and lyophilized cells on various pathogenic bacterial strains isolated from skin. The filtrate and lyophilized cell solution of L. plantarum-ATCC8014 showed various impacts on isolated bacteria from the skin. L. plantarum-ATCC8014 filtrate has the highest inhibition zones versus all tested bacteria compared to lyophilized cell solution, whereas it showed the highest action toward S. aureus which had been selected to induce infection, as well as comparing the anti-inflammatory impact of both forms. Five animal groups were tested after the induction of a Staphylococcus aureus wound infection model and monitored for comparative evaluation of the beneficial impact of L. plantarum-prepared forms in the healing process. L. plantarum filtrate has higher antibacterial action compared to lyophilized cells as well as an anti-inflammatory value of 12.7 ± 2.1 µg/ml. Furthermore, L. plantarum filtrate accelerates wound healing, which is indirectly related to animals’ body weight. Microbiological counting of bacterial load in skin and internal organs, including the liver and spleen, revealed that treatment with L. plantarum filtrate led to a notable reduction in bacterial load and prevented its dissemination. Histological examination confirmed the impact of the prepared filter in controlling scars and normalized mast cells, which regulate inflammation. Inflammatory cytokines and oxidative enzyme testing in all groups reflected the protective roles of L. plantarum filtrate with minimal burden on body functions.","PeriodicalId":11512,"journal":{"name":"Egyptian Journal of Basic and Applied Sciences","volume":"43 19","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135820347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Computational examination to reveal Kaempferol as the most potent active compound from Euphorbia hirta against breast cancer by targeting AKT1 and ERα 计算检验揭示山奈酚是大大麻中针对AKT1和ERα的最有效的抗乳腺癌活性化合物

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-10-31 DOI: 10.1080/2314808x.2023.2272385

Muhammad Hermawan Widyananda, Fatchiyah Fatchiyah, Lailil Muflikhah, Siti Mariyah Ulfa, Nashi Widodo

引用次数: 0

Implications of Multimerin-1 (MMRN-1) expression in adult de novo acute myeloid leukemia: a preliminary study in Delta Egypt 多聚蛋白-1 (MMRN-1)表达在成人新生急性髓性白血病中的意义:埃及三角洲地区的初步研究

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-09-27 DOI: 10.1080/2314808x.2023.2252233

Nadia El Menshawy, Mohamed S. El-Goneimy, Hyam Fathi, Maha Saif, Heba Hashem

引用次数: 0

Therapeutic potential of bitter taste receptor agonists on amyloid β-induced rat model of Alzheimer’s disease 苦味受体激动剂对β淀粉样蛋白诱导的老年痴呆症大鼠模型的治疗潜力

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-09-25 DOI: 10.1080/2314808x.2023.2252635

Sara F. Khalifa, Fathy M. El-Taweel, Mohammed F. Salama, Mohammed A. El-Magd

{"title":"Therapeutic potential of bitter taste receptor agonists on amyloid β-induced rat model of Alzheimer’s disease","authors":"Sara F. Khalifa, Fathy M. El-Taweel, Mohammed F. Salama, Mohammed A. El-Magd","doi":"10.1080/2314808x.2023.2252635","DOIUrl":"https://doi.org/10.1080/2314808x.2023.2252635","url":null,"abstract":"This study was conducted to investigate the effect of bitter taste receptor (T2R) agonists (caffeine and extracts of myrrh and Boswellia serrata) on amyloid β (Aβ)-induced Alzheimer’s disease (AD) in rats. Rats intracerebroventricularly (ICV) injected with a single dose of Aβ (3 μg/μL/rat) had significantly: 1) lower brain weight, 2) higher short-term memory impairment, 3) lower serum dopamine level, 4) higher AChE activity, 5) higher brain lipid peroxide malonaldehyde, 6) lower brain antioxidant activities of catalase, superoxide dismutase and glutathione peroxidase, 7) higher brain phosphor tau protein level, 8) higher brain levels of apoptotic markers (Bax and caspase 3), 9) lower brain level of antiapoptotic marker Bcl2, 10) downregulated brain expression of the bitter receptor (T2R4) gene, 11) upregulated brain expression of tumor necrosis factor α (TNFα) and nuclear factor κB (NF-κB) genes and 12) marked neuronal degeneration associated with severe vacuolation of neutrophils. All deteriorated effects of Aβ were restored following treatment with caffeine (20 mg/kg), myrrh aqueous extract (10 mg/kg) and Boswellia serrata aqueous extract (400 mg/kg) with best effect for myrrh aqueous extract. These data conclude that bitter taste receptor agonists (caffeine and extracts of myrrh and Boswellia serrata) had therapeutic potential on amyloid β-induced rat model of Alzheimer’s disease.","PeriodicalId":11512,"journal":{"name":"Egyptian Journal of Basic and Applied Sciences","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135817217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Taurine and N -acetylcysteine reverse reproductive and neuroendocrine dysfunctions in levetiracetam-treated epileptic male rats 牛磺酸和N -乙酰半胱氨酸逆转左乙西坦治疗癫痫雄性大鼠的生殖和神经内分泌功能障碍

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-09-21 DOI: 10.1080/2314808x.2023.2254529

Emojevwe Victor, Oyovwi Mega Obukohwo, Naiho Obidike Alexander, Osigwe Elect Chinaecherem, Annonye Victoria Obianuju, Nwangwa Eze Kingsley, Ben-Azu Benneth, Enemali Felix, Uwejigho Raphael, Oghenetega Onome Bright, Ogunmiluyi Olufunmbi Ebenezer, Joseph Gregory Uchechukwu

{"title":"Taurine and N -acetylcysteine reverse reproductive and neuroendocrine dysfunctions in levetiracetam-treated epileptic male rats","authors":"Emojevwe Victor, Oyovwi Mega Obukohwo, Naiho Obidike Alexander, Osigwe Elect Chinaecherem, Annonye Victoria Obianuju, Nwangwa Eze Kingsley, Ben-Azu Benneth, Enemali Felix, Uwejigho Raphael, Oghenetega Onome Bright, Ogunmiluyi Olufunmbi Ebenezer, Joseph Gregory Uchechukwu","doi":"10.1080/2314808x.2023.2254529","DOIUrl":"https://doi.org/10.1080/2314808x.2023.2254529","url":null,"abstract":"Reproductive neuroendocrine dysfunction is becoming common among patients with epilepsy, Notably, possible indication of taurine and N-acetylcysteine (NAC) in the management of reproductive impairment has increased over the years. However, scientific evidence to supplement our understanding of the impact of taurine and NAC in protecting reproductive neuroendocrine cells is lacking. Hence, this study aimed to investigate the possible effects of taurine and NAC on levetiracetam-induced reproductive neuroendocrine dysfunction in epileptic rats. The study was done in two experimental protocols: drug alone and reversal. In the drug-alone protocol, rats in groups 1–6 received treatment orally for 21 days, respectively. In the reversal-protocol, the animals in group 1, received saline (2.5 mL/kg) and served as normal control, while epilepsy was induced in animals in groups 2–5, after which groups 3–5 received levetiracetam (150 mg/kg), levetiracetam (150 mg/kg) + NAC (100 mg/kg), levetiracetam (150 mg/kg) + taurine (150 mg/kg) respectively for 14 days. Pilocarpine alone or in combination with levetiracetam significantly decreased neuro-endocrine related factors, hormonal profile, and spermatogenesis, whereas treatment with NAC and taurine reversed the effects induced by pilocarpinethrough neuro-endocrine-related factors and hormonal modulation mechanism.","PeriodicalId":11512,"journal":{"name":"Egyptian Journal of Basic and Applied Sciences","volume":"139 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136235778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Incidence of virulence determinants among Streptococcus agalactiae isolated from pregnant women and association with their serotypes 孕妇分离的无乳链球菌毒力决定因素的发生率及其血清型的相关性

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-09-19 DOI: 10.1080/2314808x.2023.2244772

Reham R. EL-Lakany, Eman S. Abdelmaged, Maher Shams, Ramadan Hassan, Dina E. Rizk

{"title":"Incidence of virulence determinants among Streptococcus agalactiae isolated from pregnant women and association with their serotypes","authors":"Reham R. EL-Lakany, Eman S. Abdelmaged, Maher Shams, Ramadan Hassan, Dina E. Rizk","doi":"10.1080/2314808x.2023.2244772","DOIUrl":"https://doi.org/10.1080/2314808x.2023.2244772","url":null,"abstract":"Colonization of pregnant women by Streptococcus agalactiae (S. agalactiae) is the main reason for intrauterine infection or transmission during parturition. This study investigated the prevalence, antimicrobial susceptibility, serotypes, virulence traits of S. agalactiae in pregnant women across three hospitals in Dakahlia Governorate, Egypt. Isolates were identified by culturing onto Hichrome Sterp B selective medium, latex agglutination and biochemical tests. The antimicrobial susceptibility was determined by Kirby-Bauer disc diffusion method. Capsular serotypes were determined by latex agglutination test. Hemolysin production and biofilm formation were assessed quantitively. Virulence genes were detected by polymerase chain reaction (PCR). Among 290 samples, 31.7% were S. agalactiae. All isolates were sensitive to penicillin G, ampicillin, cefotaxime, and vancomycin, but notably resistant to tetracycline (83.6%), erythromycin (68.4%), and clindamycin (54.3%). Serotype III (26.08%) was most common. Strong biofilm was produced by 72.8% of isolates. High hemolytic activity was seen (80.4%). Prevalent virulence genes included fsbA (97.8%), fbsB (96.7%), and scpB (96.7%). rib and alp4 were frequent surface protein genes (60.8% and 57.6%, respectively). Serotypes were significantly correlated to clindamycin resistance, biofilm production (Ia, III, VI serotypes), hemolytic activity (serotype V), and several virulence genes and surface proteins. Our study found a high prevalence of S. agalactiae isolates in pregnant women with diverse resistance patterns, high virulence indicating potential for increased pathogenicity.","PeriodicalId":11512,"journal":{"name":"Egyptian Journal of Basic and Applied Sciences","volume":"198 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135015908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The association of Sirtuin1 (SIRT1) polymorphism and downregulation of STAT4 gene expression with increased susceptibility to COVID-19 infection SIRT1多态性和STAT4基因表达下调与COVID-19感染易感性增加的关系

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-09-11 DOI: 10.1080/2314808x.2023.2254507

Nora Mostafa, May Elsherbiny Badr, Olfat G. Shaker, Ghada Elsaid, Rasha Samir Shemies, Doaa Khedr, Hend Gamal Abuelfadl, Mona Elhelaly Elsherbeny

{"title":"The association of Sirtuin1 (SIRT1) polymorphism and downregulation of STAT4 gene expression with increased susceptibility to COVID-19 infection","authors":"Nora Mostafa, May Elsherbiny Badr, Olfat G. Shaker, Ghada Elsaid, Rasha Samir Shemies, Doaa Khedr, Hend Gamal Abuelfadl, Mona Elhelaly Elsherbeny","doi":"10.1080/2314808x.2023.2254507","DOIUrl":"https://doi.org/10.1080/2314808x.2023.2254507","url":null,"abstract":"Sirtuin1 (SIRT1) is an epigenetic modulator that belongs to sirtuins family and participates in many physiologic reactions, as genomic stabilization, apoptosis, proliferation, and inflammation. STAT4 (signal transducer and activator of transcription 4) gene is a component of JAK-STAT signaling pathway, which plays key role in activating cellular-mediated immune responses. The present study aimed to investigate the association between SIRT1 rs12778366 SNP, expression level of STAT4 gene and susceptibility to COVID-19 infections as well as their correlation to clinicopathological data.The present study included 100 ICU patients with severe COVID-19 infection and 100 age- and sex-matched healthy controls. DNA was extracted. Genotyping of SNP in SIRT1 (rs12778366) was performed, Total RNA was extracted from PBMCs. Reverse transcription was done. STAT4 gene expression levels were evaluated with GAPDH as internal control using real-time PCR. We found a significantly higher frequency of ‘C’ allele and C/T genotype in case vs. control. The association was of low strength (φ = 0.105 for alleles, and 0.154 for genotypes). This was associated with higher expression of STAT4 gene (P < 0.001) and increased tendency for lower respiratory complications. Median STAT4 (FC) (Median = 0.18, 95% CI = 0.15–0.27) was lower than normal control value of 1.0. This difference was statistically significant (Hodges-Lehmann location estimator = 0.217, P < 0.001). SIRT1 polymorphism and decreased expression of STAT4 gene are associated with increased susceptibility to COVID-19 infection and correlate to its phenotypic manifestations.","PeriodicalId":11512,"journal":{"name":"Egyptian Journal of Basic and Applied Sciences","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135981220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of IL28B (rs8099917) gene polymorphism on treatment response to Sovaldi, Daklinza, and Ribavirin in Egyptian patients with HCV infection IL28B (rs8099917)基因多态性对埃及HCV感染患者Sovaldi、Daklinza和利巴韦林治疗反应的影响

Egyptian Journal of Basic and Applied Sciences Pub Date : 2023-09-11 DOI: 10.1080/2314808x.2023.2254508

Mohammed A. Eid, Azza El-Sayed Ali Youssef, Niveen Salah El-Din Saudy, Anwar S. El-Badry

{"title":"Impact of IL28B (rs8099917) gene polymorphism on treatment response to Sovaldi, Daklinza, and Ribavirin in Egyptian patients with HCV infection","authors":"Mohammed A. Eid, Azza El-Sayed Ali Youssef, Niveen Salah El-Din Saudy, Anwar S. El-Badry","doi":"10.1080/2314808x.2023.2254508","DOIUrl":"https://doi.org/10.1080/2314808x.2023.2254508","url":null,"abstract":"Infection by the hepatitis C virus (HCV) is a big threat to human health all over the world. Many host factors could affect the treatment response in HCV infection. One of these factors is IL28B gene polymorphism. This research study investigated the association of IL28B (rs8099917) gene polymorphism with treatment response in patients with chronic hepatitis C virus (HCV) infection receiving a combination of Sovaldi, Daklinza, and Ribavirin in Egypt. The study included 100 patients, divided into three groups based on treatment outcomes: responders, relapsers, and non-responders. Biochemical and hematological analyses were performed, and HCV genotypes were determined using PCR. IL28B genotyping was done using real-time PCR. The results showed a high prevalence of HCV genotype 4 and a few patients with genotype 1. The G allele of IL28B was associated with a higher risk of developing relapse and non-response, while the TT genotype was significantly associated with treatment success. Viral load levels were lower in responders compared to relapsers and non-responders. The study suggests that IL28B genotype may be a valuable predictor of treatment response in chronic HCV patients treated with the specified combination therapy. Further research is needed to confirm and fully understand these findings.","PeriodicalId":11512,"journal":{"name":"Egyptian Journal of Basic and Applied Sciences","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135981204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0