Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences最新文献_第4页

MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA 从印度尼西亚西爪哇几个养鸡场分离出耐多药沙门氏菌

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-04-14 DOI: 10.21157/j.ked.hewan.v16i1.18944

Aprilia Hardiati, Safika Safika, F. Pasaribu, I. Wibawan

{"title":"MULTIDRUG-RESISTANT Salmonella sp. ISOLATED FROM SEVERAL CHICKEN FARMS IN WEST JAVA, INDONESIA","authors":"Aprilia Hardiati, Safika Safika, F. Pasaribu, I. Wibawan","doi":"10.21157/j.ked.hewan.v16i1.18944","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i1.18944","url":null,"abstract":"This study was aimed at isolating and identifying Salmonella sp. and then conducting an antibiotics susceptibility test in order to detect resistant genes. One hundred and five chicken cloaca swab samples were used in this study. 30 samples were taken from a layer farm in Bogor, 45 from a broiler farm in Sukabumi and 30 from a broiler farm in Cianjur. In order to isolate and identify the bacteria, a tetrathionate broth was used, which was then cultured in a Salmonella-Shigella agar, and finally a Gram stain and biochemical test was conducted. To confirm the presence of Salmonella sp., a pair of primers were used for the polymerase chain reaction (PCR) method to determine the presence of the invA gene.. An antibiotics susceptibility test was used with the Kirby-Bauer disk diffusion method. Nine antibiotics were used in this study. Each primer pair was used for the detection of tetA, blaTEM, aac(3)-IV, gyrA and ermB genes, and for genes encoding antibiotic resistance a PCR test was used. Eight (7.6%) Salmonella sp. were isolated in this study. All isolates showed positive results with PCR confirmation. The results of the antibiotics susceptibility test showed that Salmonella sp. isolates were resistant to tetracycline (75%), oxytetracycline (75%), amphicillin (75%), gentamycin (12.5%), nalidixic acid (100%), ciprofloxacin (12.5%), enrofloxacin (0%), erythromycin (100%), and chloramphenicol (0%). The distribution of antibiotic resistance genes in Salmonella sp. were tetA (33.3%), blaTEM (100%), aac(3)-IV (0%), gyrA (100%) and ermB (0%) positive. In conclusion, Salmonella sp. was isolated. All isolates showed positive results in the PCR confirmation. Salmonella sp. isolates were resistant to tetracycline, oxytetracycline, amphicillin, gentamycin, nalidixic acid, ciprofloxacin, and erythromycin. Only the tetA, blaTEM, and gyrA genes were detected in Salmonella sp. isolates.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116474871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

ANTICANCER ACTIVITY OF ETHANOL EXTRACT OF YELLOW ROOT (Arcangelisia flava) ON HEPG2 HEPATOCELLULAR CANCER CELLS 黄根乙醇提取物对HEPG2肝癌细胞的抗癌作用

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-04-14 DOI: 10.21157/j.ked.hewan.v16i1.23615

H. Yusuf, Marhami Fahriani, Cut Murzalina

{"title":"ANTICANCER ACTIVITY OF ETHANOL EXTRACT OF YELLOW ROOT (Arcangelisia flava) ON HEPG2 HEPATOCELLULAR CANCER CELLS","authors":"H. Yusuf, Marhami Fahriani, Cut Murzalina","doi":"10.21157/j.ked.hewan.v16i1.23615","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i1.23615","url":null,"abstract":"This study aimed to evaluate anticancer activity and apoptosis induction of ethanolic extract of Arcalengisia flava (AF) roots on HepG2 cancer cell lines. The AF roots were extracted by maceration using ethanol 80%. MTT assay method was used to evaluate the anticancer activity and the proliferation of HepG2 cells. Flow cytometry method was used to assess the induction of HepG2 cells apoptosis. This study showed that the IC50 of AF ethanol extract against HepG2 cells was 109.14 μg/ml. With IC50 treatment, the apoptosis assay showed a significant decrease in intact cells (80.10±1.7%) and a significant increase in early apoptosis (7.9±0.7%) and late apoptosis cells of HepG2 cancer cells (4.9±0.35%) compared to control cells. Moreover, the proliferation of HepG2 cells was declined significantly in 48 and 72 hours after treatment with IC50 (77.5±5.76% and 64.3±5.37%, respectively) and 2xIC50 of the extract (75.9±1.79% and 70.5±4.27%, respectively). This research suggests that the ethanolic extract of AF roots can potentially be used for hepatocarcinoma treatment.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"63 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131602251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CANINE LEPTOSPIROSIS IDENTIFICATION IN SMALL ANIMAL PRACTICE 小动物钩端螺旋体病鉴定

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-04-14 DOI: 10.21157/j.ked.hewan.v16i1.20936

E. Sulistiawati, Zulfitra Utami Putri, C. K. Sajuthi, D. Sajuthi, Nurul Oktaviani

{"title":"CANINE LEPTOSPIROSIS IDENTIFICATION IN SMALL ANIMAL PRACTICE","authors":"E. Sulistiawati, Zulfitra Utami Putri, C. K. Sajuthi, D. Sajuthi, Nurul Oktaviani","doi":"10.21157/j.ked.hewan.v16i1.20936","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i1.20936","url":null,"abstract":"Leptospirosis is an infectious disease caused by bacteria from the genus Leptospira with various serovars and often presents with a wide range of clinical signs from mild to severe conditions, particularly it presents with a mild fever to asymptomatic infection-causing animal death. The purpose of this study was to identify the characteristics of canine leptospirosis through laboratory examinations supported by medical record data in a small animal practice at the drh Cucu K. Sajuthi Joint Veterinary Practice, in Jakarta. The study was conducted on serum samples of sixteen dogs from drh Cucu K. Sajuthi Joint Veterinary Practice patients, which presented varied clinical signs of leptospirosis, and which had been accompanied by hematological examination, blood biochemistry, and Microscopic Agglutination Test (MAT) against various serovars such as Bataviae, Icterohaemorrhagiae, and Javanica. The collected data was analyzed descriptively. The results showed that canine leptospirosis was more commonly derived from Bataviae serovar and was accompanied by clinical signs such as vomiting, jaundice, anorexia, abdominal pain, lethargy, pale mucosal membrane, diarrhea, dehydration, dyspnea, and polyuria/polydipsia. A predisposition of gender and age was commonly found in male dogs less than 5 years old. Abnormalities found from hematological examination were leukocytosis, granulocytosis (neutrophilia), anemia, thrombocytopenia, and lymphopenia, while blood chemistry showed azotemia, an increase in liver enzymes (AST, ALT, ALP), hyperbilirubinemia, hyponatremia, hyperphosphatemia, and hypoalbuminemia.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128911788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHARACTERIZATION OF GLYCOCUNJUGATE DISTRIBUTION IN THE EPIDIDYMIS OF THE JAVAN MUNTJACS (Muntiacus muntjak muntjak) 爪哇猕猴附睾糖偶联物的分布特征

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-04-14 DOI: 10.21157/j.ked.hewan.v16i1.24418

S. Wahyuni, H. Sofyan, H. Hafizuddin, I. K. M. Adnyane, G. Gholib, M. Jalaluddin, Fadli A. Gani, M. Adam, T. Siregar, S. Agungpriyono

{"title":"CHARACTERIZATION OF GLYCOCUNJUGATE DISTRIBUTION IN THE EPIDIDYMIS OF THE JAVAN MUNTJACS (Muntiacus muntjak muntjak)","authors":"S. Wahyuni, H. Sofyan, H. Hafizuddin, I. K. M. Adnyane, G. Gholib, M. Jalaluddin, Fadli A. Gani, M. Adam, T. Siregar, S. Agungpriyono","doi":"10.21157/j.ked.hewan.v16i1.24418","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i1.24418","url":null,"abstract":"The presence of glycoconjugates in caput, corpus, and cauda epididymis of the Javan muntjacs was performed using lectin histochemical method. The epididymis organs were obtained from two adult male Javan muntjacs in hard antler stage and then immediately fixed in Bouin’s solution and processed in histological manner. Six types of biotinylated lectins namely Peanut agglutinin (PNA), Soybean agglutinin (SBA), Wheat germ agglutinin (WGA), Ricinus communis agglutinin (RCA), Concanavalin A (Con A), and Ulex europaeus agglutinin I (UEA I) were applied to detect the specific glycoconjugates in the epididymal tissue of muntjacs. The result showed the presence of glycoconjugates were detected by five types of lectins except UEA I in caput, corpus, and cauda epididymis with specific distribution and also the intensity of lectins binding. Glycoconjugates β-galactose, β-glucose, mannose, N-acetyl-galactosamine, N-acetyl-glucosamine, and sialic acid were stained with various intensity in epithelial cells, basal cells, stereocilia, spermatozoa, and stroma of caput, corpus, and cauda epididymis. The presence of sialic acid on the surface of spermatozoa detected by WGA are thought to be play a role on sperm protection during their maturation and transit along epididymal lumen. In conclusion, the difference of glycoconjugates distribution pattern and lectin binding in the epididymal caput, corpus, and cauda of the Javan muntjac indicates the regional differences in epididymal function in spermatozoa maturation.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"188 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114444323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CLOSE POSITIVE ASSOCIATION BETWEEN OBESITY AND BLOOD PRESSURE IN RATS 大鼠肥胖和血压之间的密切正相关

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-04-14 DOI: 10.21157/j.ked.hewan.v16i1.23913

Yusni Yusni, H. Yusuf

{"title":"A CLOSE POSITIVE ASSOCIATION BETWEEN OBESITY AND BLOOD PRESSURE IN RATS","authors":"Yusni Yusni, H. Yusuf","doi":"10.21157/j.ked.hewan.v16i1.23913","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i1.23913","url":null,"abstract":"The purpose of this study was to analyze how the effect of each increase in Body Mass Index (BMI) on the increase in blood pressure (BP). The research design was a laboratory experiment with 52 non-obese and obese rats as subjects (non-obese, n=14 and obese, n=38). Rats were induced obesity using a high-fat diet for four weeks. BMI used is based on the Lee Index with obesity indicators if 300 g/cm. Data analysis using independent sample t-test, simple linear regression test, and Pearson correlation test; (p0.05). The results: BP in non-obese rats was lower than obese rats (89.00±7.38 vs 190.11±4.42; p=0.001*), BMI was positively associated with BP (p=0.001*); an increase in BMI will increase BP (p=0.001*), there is a strong correlation between BMI and BP (r=0.977; p=0.001*). The regression equation (Y = 707.42 + 2.75 X) indicates that every 1 g/cm increase in BMI will be followed by an increase in BP of 2.75 mmHg. The analysis of R-square = 0.942 shows that the accuracy of the linear equation model is 94.2%. In conclusion, BMI has a strong positive correlation with BP, an increase in BMI will increase BP; obesity induces high blood pressure in rats.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121918005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

POPULATION OF Ascaridia galli ACCORDING TO ITS PREDILECTION AFTER BEING TREATED WITH ARECA NUT INFUSION AND ITS ECONOMIC ANALYSIS 槟榔冲剂处理后鸡蛔虫的种群偏好及其经济分析

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-04-14 DOI: 10.21157/j.ked.hewan.v16i1.24968

B. Sudarmanto, Muzizat Akbarrizki, Wida Wahidah Mubarokah

{"title":"POPULATION OF Ascaridia galli ACCORDING TO ITS PREDILECTION AFTER BEING TREATED WITH ARECA NUT INFUSION AND ITS ECONOMIC ANALYSIS","authors":"B. Sudarmanto, Muzizat Akbarrizki, Wida Wahidah Mubarokah","doi":"10.21157/j.ked.hewan.v16i1.24968","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i1.24968","url":null,"abstract":"This study aims to determine the effectiveness of Areca catechu Crude Aqueous Extract (ACCAE) as an anthelmintic, the number ofAscaridia galli worms in the duodenum, jejunum, and ileum after treatment, and an economic analysis. In this study, 50 female chickens wereused. The chickens were divided into 5 groups: Treatment 1, negative control (P1), which was given aquadestylates; treatment 2 (P2), which wasgiven infusion of ACCAE at a dose of 26 mg/mL; treatment 3 (P3), which was given infusion of ACCAE at a dose of 53 mg/mL; treatment 4(P4), which was given ACCAE with a dose of 79 mg/mL; and treatment 5 (P5) (positive control with pyrantel pamoat). Each group consisted of10 chickens. An examination was carried out that included weighing the population of adult worms according to their predilection 14 days aftertreatment. Weighing was carried out at the beginning before treatment, on the 7th day after treatment and on the 14th day after treatment. Thechickens were then euthenized, the intestinal worms were counted, and the economic analysis was calculated. Based on the results of the study, itcan be concluded that the best dose for removing A. galli worms is 79 mg/mL, and the location of the predilection of the intestine where the mostworms are found is the jejunum and the least is in the ileum. Economically, the highest income was obtained from treatment 4, namely the groupwith infusion of areca nut at a dose of 79 mg/mL.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116123405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES 不同年龄西门塔尔公牛的生产力和鲜精液特性

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2022-04-14 DOI: 10.21157/j.ked.hewan.v16i1.23487

Faisal Amri Satrio, N. Karja, M. Setiadi, E. M. Kaiin, Asep Kurnia, B. Purwantara

{"title":"PRODUCTIVITY AND FRESH SEMEN CHARACTERISTICS OF SIMMENTAL BULL DIFFERENT AGES","authors":"Faisal Amri Satrio, N. Karja, M. Setiadi, E. M. Kaiin, Asep Kurnia, B. Purwantara","doi":"10.21157/j.ked.hewan.v16i1.23487","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v16i1.23487","url":null,"abstract":"This study aimed to determine the effect of age on bulls’ productivity and fresh semen characteristics of Simmental bull in Indonesia. A total of 1071 data of semen collection and production from four age groups (two years old (yo), four yo, ≥ 10 yo with high semen rejection (≥10 HR), and ≥ 10 yo with low semen rejection (≥10 LR) were used in this study to evaluate the productivity and characteristics of fresh semen. The results showed that the pre-freezing and post-freezing semen rejection rate of ≥10 HR group was higher (P0.05) than the other groups. The four yo group had the percentage of second semen ejaculation each collection was higher (P0.05) than the other groups. Furthermore, semen volume, sperm concentration, and total sperm concentration significantly increased (P0.05) until four yo and then decreased (P0.05) in ≥ 10 yo groups. The ≥10 HR group had the volume and total sperm concentration significantly different (P0.05) with a group of ≥10 LR. Total sperm motility, individual motility, and mass movement were lower (P0.05) in ≥10 HR than the other groups. In conclusion, age differences of bulls can affect the productivity and characteristics of fresh semen.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127556002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

THE EFFECTIVITY OF CYSTEAMINE SUPPLEMENTATION ON IMPROVING THE IN VITRO FERTILIZATION RATE OF SHEEP OOCYTES 补充半胱胺对提高绵羊卵母细胞体外受精率的效果

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2021-12-31 DOI: 10.21157/j.ked.hewan.v15i4.17688

Dona Astari Nurkarimah, E. M. Kaiin, N. W. Karja, M. Setiadi

{"title":"THE EFFECTIVITY OF CYSTEAMINE SUPPLEMENTATION ON IMPROVING THE IN VITRO FERTILIZATION RATE OF SHEEP OOCYTES","authors":"Dona Astari Nurkarimah, E. M. Kaiin, N. W. Karja, M. Setiadi","doi":"10.21157/j.ked.hewan.v15i4.17688","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v15i4.17688","url":null,"abstract":"The purpose of this study was to evaluate the effectiveness of cysteamine supplementation on the maturation medium and/or in vitro fertilization medium with regards to improving the normal fertilization rate of sheep oocytes, which are characterized by the formation of two pronuclei. Grade A and B oocytes were matured in medium-199 with 0.3% bovine serum albumin (BSA), 10 IU/mL follicle stimulating hormone (FSH), 10 IU/mL human chorionic gonadotrophin (hCG), and 50 μg/mL gentamicin added for 24 hours in a 5% CO2 incubator at 39° C. The treatment group was divided into the following groups: a control group with no cysteamine (P1), a group with 100 μM cysteamine in the maturation medium (P2), a group with 100 μM cysteamine in the fertilization medium (P3), and a group with 100 μM cysteamine both in the maturation and fertilization medium (P4). The fertilization was carried out by incubating sperm-oocytes for 12 hours and then staining them with 1% aceto-orcein to observe the formation of a pronucleus. Normal fertilization rates obtained by each treatment group were 56.5% (P1), 57.1% (P2), 57.8% (P3), and 59.9% (P4) with no significant difference (P0.05) between groups. It was concluded that 100 μM cysteamine supplementation in both the maturation medium and fertilization medium was not able to increase the normal fertilization rate of sheep oocytes.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114746192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DETERMINATION OF THE BEST ROSELLA (Hibiscus sabdarifa L.) FILTRATE CONCENTRATION IN EGG YOLK CITRATE DILUENT 最佳玫瑰花(芙蓉)的测定蛋黄柠檬酸稀释液滤液浓度

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2021-12-31 DOI: 10.21157/j.ked.hewan.v15i4.22398

A. Sayuti, H. Mayuri., Nuzul Asmilia, Tazul Arifin, M. Adam

{"title":"DETERMINATION OF THE BEST ROSELLA (Hibiscus sabdarifa L.) FILTRATE CONCENTRATION IN EGG YOLK CITRATE DILUENT","authors":"A. Sayuti, H. Mayuri., Nuzul Asmilia, Tazul Arifin, M. Adam","doi":"10.21157/j.ked.hewan.v15i4.22398","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v15i4.22398","url":null,"abstract":"The aim of this research was to determine if the substitution of rosella filtrate (Hibiscus sabdarifa L.) in citrate-based diluent and egg yolks with the ratio 4:1 (16 mL citrate + 4 mL yolk) would help maintain the progressive motility of Kacang goat spermatozoa at room temperature. This study used a male Kacang goat with an average sperm quality of approximately ≥ 205 x 107 spermatozoa/mL and motility of 81.6%. An electro-ejaculator was used once a week to collect the semen. The treatments were labelled as follows: P0 was the negative control group, treatment P1 was given 5% rosella filtrate, treatment P2 was given 10% rosella filtrate, and treatment P3 was given 15% rosella filtrate. Examination of the motility of the spermatozoa was carried out at room temperature. The examination was carried out once every four hours. It was concluded that the rosella filtrate could maintain the progressive motility of spermatozoa of the Kacang goat at optimal concentrations. The optimal motility percentage was found at a concentration of 15% at a storage life of 0-4 hours.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126570414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAUSES AND REMEDIES FOR NON-INFECTIOUS INFERTILITY IN SHEEP AND GOATS 绵羊和山羊非感染性不孕的原因和治疗方法

Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences Pub Date : 2021-12-31 DOI: 10.21157/j.ked.hewan.v15i4.20795

Ö. Uçar, B. Uslu

{"title":"CAUSES AND REMEDIES FOR NON-INFECTIOUS INFERTILITY IN SHEEP AND GOATS","authors":"Ö. Uçar, B. Uslu","doi":"10.21157/j.ked.hewan.v15i4.20795","DOIUrl":"https://doi.org/10.21157/j.ked.hewan.v15i4.20795","url":null,"abstract":"Non-infectious infertility is particularly critical for sire rams/bucks, as seasonal breeders. This seasonal heavy use of males may lead to sexual exhaustion such that the regular semen production, libido, ejaculation, and litter yield can be seriously impaired. In severe cases, the reproductive processes may stop completely. For avoiding infertility risk, management and feeding should firstly be arranged with a flock-based approach. In reproductive management, the issues of using teaser ram/buck, male/female ratio, weekly mating number, exercise, and experience need attention. Also, inbreeding and using old breeders should be avoided. In oestrus synchronisation, necessary precautions against extremely hot/arid climatic conditions should be considered together with a sufficient number of rams/bucks used rotationally. For sustainable sheep/goat breeding, protective-preventive measures are preferred to problem-solving approaches. In enterprises where female breeder welfare is a priority, sustainable individual yield increases. Further, only the comfort level of males used heavily in season allows the expected libido and fertility to occur. In small ruminants, the necessary interest and responsibility in production provide convenience in the ideal solution with the opportunity of early interventions against undesirable fertility problems in breeders. Undoubtedly, it is vital to consider sustainable animal health and welfare for profitable breeding and husbandry in sheep/goats.","PeriodicalId":114727,"journal":{"name":"Jurnal Kedokteran Hewan - Indonesian Journal of Veterinary Sciences","volume":"194 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121122944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0