Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy最新文献_第10页

Fecundity in autoimmune diseases. 自身免疫性疾病的繁殖力

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-06-01

J Cohen, R Bakimer, Y Shoenfeld

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Biochemical and sonographic evaluation of the very early intrauterine pregnancy. 早期宫内妊娠的生化及超声评价。

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-06-01

A B Copperman, M Bustillo

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Does occupational nuclear power plant radiation affect conception and pregnancy? 职业性核电站辐射会影响受孕和怀孕吗?

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-06-01

E Straube, W Straube, T Römer

{"title":"Does occupational nuclear power plant radiation affect conception and pregnancy?","authors":"E Straube, W Straube, T Römer","doi":"","DOIUrl":"","url":null,"abstract":"Objectives: To determine the effect of occupational exposure in a nuclear power plant in Griefswald, Germany on male and female fecundity.Methods: The frequency of men and women exposed to ionizing radiation through work in a nuclear power plant among 270 infertile couples was retrospectively compared to a control fertile population using a pair-matched analysis. The total cumulative equivalent radiation dose was determined. In addition, the spermiograms of the male partners in both groups were compared and correlated to the degree of exposure.Results: No differences were noted in the frequency of nuclear power plant exposure between sterile and fertile groups. There was a higher rate of anomalous spermiograms in nuclear power plant workers. However, abnormalities were temporary. No correlation was found between the cumulative equivalent radiation dose and abnormal spermiograms.Conclusion: The data suggest that occupational exposure due to ionizing radiation should be investigated as a possible cause for involuntary temporary sterility and as a risk factor for early pregnancy disorders.","PeriodicalId":11444,"journal":{"name":"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20292486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of endometrial placental protein 14 and prolactin by cultured endometrial explants after collagenase and freeze/thaw treatment, and in response to progesterone. 体外培养的子宫内膜外植体经胶原酶和冻融处理后产生胎盘蛋白14和催乳素，并对黄体酮作出反应。

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-06-01

N A Bersinger, A W Brandenberger, A Zakher, E Dreher, H J Altermatt, M H Birkhäuser

{"title":"Production of endometrial placental protein 14 and prolactin by cultured endometrial explants after collagenase and freeze/thaw treatment, and in response to progesterone.","authors":"N A Bersinger, A W Brandenberger, A Zakher, E Dreher, H J Altermatt, M H Birkhäuser","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Investigation of methods for maintaining functional endometrial explants in culture after cryopreservation with or without previous enzymatic dispersion of stromal cells and epithelial glands. Such a standardized culture system is a requirement for the development of a non-invasive bioassay for embryo quality in in vitro fertilization programs, a method that will eventually measure endometrial response to embryo conditioned media.Method: Culture of mid-luteal phase endometrial biopsies, in the presence of [35S]methionine, with or without prior collagenase treatment and/or storage in liquid nitrogen in the presence of dimethyl sulfoxide. Determination of released de novo synthesized total protein by trichloroacetic acid precipitation of culture media. Measurement, after culture in absence and presence of progesterone, of prolactin and placental protein 14 (PP14) production by sensitive non-isotopic immunoassays.Results: Production of prolactin, but not PP14, was increased by 200 nmol/l progesterone, 2-8-fold after 4 days and 1.5-700-fold after 7 days in culture. After limited collagenase treatment (but without separation of stromal cells from glands), both marker protein productions were similar compared to untreated explants; however, there was no significant stimulation of prolactin by progesterone. After freezing and thawing, production was markedly reduced, particularly from explants frozen following collagenase treatment.Conclusions: Both stromal and glandular viability are maintained after collagenase treatment but the response to progesterone is lost. Cryopreservation reduced prolactin and PP14 production in subsequent culture. Therefore, novel freezing protocols should be developed which preserve both endometrial structure and function.","PeriodicalId":11444,"journal":{"name":"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20292487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A non-trophoblastic tumor co-existing with a triploid fetus. 与三倍体胎儿共存的非滋养细胞肿瘤。

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-03-01

M Pietrantoni, C K Brees, A Gerassimides, V Cook, B Youkilis, J H Hersh

{"title":"A non-trophoblastic tumor co-existing with a triploid fetus.","authors":"M Pietrantoni, C K Brees, A Gerassimides, V Cook, B Youkilis, J H Hersh","doi":"","DOIUrl":"","url":null,"abstract":"Non-trophoblastic neoplasms are the most frequent, benign tumors of the placenta, occurring in approximately 1% of all placentas examined. A case is described of a 24-year-old woman who presented with severe, early-onset pre-eclampsia, high human chorionic gonadotropin (hCG) levels, and a triploid fetus and who was found to have a small choriohemangioma. The woman, gravida 2 para 1, was referred to our hospital for perinatal evaluation. The fetus, gestational age 18 weeks 3 days, had fetal growth restriction with multiple congenital anomalies. The fetal karyotype was 69,XXY. Compared with the normal range for this gestational age, the beta-hCG level was significantly elevated (1,054,000 mIU/ml) as was the maternal serum alpha-feto-protein measurement (539.1 ng/ml). Sonographically, the placenta appeared hydropic, irregularly shaped, and gelatinous. A suction dilatation and evacuation under sonographic guidance was performed. Histological examination of placental tissue revealed hydropic degeneration of the chorionic villi. The specific histological features of a partial molar pregnancy were not present. However, there were changes consistent with a choriohemangioma. Flow cytometric DNA analysis performed on formalin-fixed, paraffin-embedded tissue blocks of placenta showed triploidy. Immunohistochemical staining with human placental alkaline phosphatase was consistent with a hydropic degeneration pattern. We conclude, first, that triploidy does not always imply the presence of a partial mole. Second, the dictum, that pre-eclampsia, if it occurs under 20 weeks' gestation, must be associated with a molar pregnancy, may not hold when placental aneuploidy is present. Although the findings in this pregnancy could have been incidental, there may be an association between a choriohemangioma and polyploidy.","PeriodicalId":11444,"journal":{"name":"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20292480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human trophoblast interferons: production and possible roles in early pregnancy. 人滋养细胞干扰素:在妊娠早期的产生和可能的作用。

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-03-01

G Aboagye-Mathiesen, F D Tóth, M Zdravkovic, P Ebbesen

{"title":"Human trophoblast interferons: production and possible roles in early pregnancy.","authors":"G Aboagye-Mathiesen, F D Tóth, M Zdravkovic, P Ebbesen","doi":"","DOIUrl":"","url":null,"abstract":"Human villous and extravillous trophoblast populations were isolated from first- and third-trimester placentae and were stimulated with viral and non-viral inducers to produce interferons (IFNs). Polyriboinosinic/polyribocytidylic acid [poly(I:C)] induced exclusively IFN-beta in trophoblast cultures, whereas viruses induced mixtures of IFN-alpha subtypes and -beta. The level of IFN production was dependent on the trophoblast population, type of inducer and the stage of differentiation of the trophoblast. First-trimester extravillous trophoblast cultures produced greater than five-fold more IFN than third-trimester villous trophoblast on a per cell basis, whereas term syncytiotrophoblast produced twice as much IFN as term mononuclear villous trophoblast when stimulated with the same inducer. Pretreatment of trophoblast cultures with platelet-derived growth factor and granulocyte/macrophage-colony stimulating factor (GM-CSF) increased the trophoblast IFN production. Tandem high-performance affinity chromatography of the virus-induced trophoblast IFNs resulted in the isolation of trophoblast IFN-alpha and -beta with specific antiviral activities of 0.75-2.73 x 10(8) IU/ml protein. The trophoblast-induced IFNs have antiproliferative and immunosuppressive properties, and, furthermore, activated natural killer cell activity. These data may suggest the possible roles of these IFNs during embryonic development with regard to protection of the fetus against viral infection and maternal immunity.","PeriodicalId":11444,"journal":{"name":"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20292560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunotherapy for recurrent spontaneous abortion. 复发性自然流产的免疫治疗。

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-03-01

C B Coulam

{"title":"Immunotherapy for recurrent spontaneous abortion.","authors":"C B Coulam","doi":"","DOIUrl":"","url":null,"abstract":"Recurrent pregnancy loss is a healthcare concern. Safe and effective treatments are necessary. Since women experiencing recurrent pregnancy loss are a heterogeneous population, specific markers are necessary to identify those who will respond to various treatments. The presence of antiphospholipid antibodies identifies women with recurrent pregnancy loss who are most likely to respond to heparin and aspirin treatment. An elevated concentration of NK cells in maternal blood and a loss of karyotypically normal embryos after detection of cardiac activity on ultrasonographic examination identify women who are most likely to respond to IVIg treatment. An obstetric history of recurrent primary abortion with an absence of maternal antipaternal lymphocytotoxic antibodies and anti-phospholipid antibodies predicts women who are most likely to respond to allogeneic leukocyte immunization. However, the treatment effect is low, with a livebirth rate of 60% which represents an enhancement over no treatment in the range of 8-10%. The difference in livebirth rates between women receiving IVIg therapy as compared to placebo was 28%. Women experiencing recurrent spontaneous abortion who have high, as opposed to low levels of leukocyte antibody do not respond to leukocyte immunization therapy. They do, however, respond to treatment with IVIg--the overall success rate of IVIg being 70%. It is important to be able to identify women likely to respond to various forms of immunotherapy. Chromosomal abnormalities are evident in 60% of recurrent aborters. Women experiencing recurrent aneuploidy in their abortus would not be expected to respond to immunotherapy. At the present time, the only way to identify such women is to have the results of chromosome analysis of previous pregnancy losses available. Having access to this information will require a change in current obstetric practice regarding obtaining karyotyping of all pregnancy losses. The cost-effectiveness of chromosome studies from abortuses is apparent when costs of evaluation and treatment are considered.","PeriodicalId":11444,"journal":{"name":"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20292556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New frontiers in early pregnancy investigation. 早期妊娠研究的新领域。

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-03-01

E R Barnea

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Will PAPP-A be a biochemical marker for screening of Down's syndrome in the first trimester? pap - a会成为筛查妊娠早期唐氏综合征的生化标志物吗?

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-03-01

K el Farra, J G Grudzinskas

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Insulin-like growth factor-I as a local regulator of proliferation and differentiated function of the human trophoblast in early pregnancy. 胰岛素样生长因子- 1在妊娠早期人滋养细胞增殖和分化功能中的局部调控作用。

Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy Pub Date : 1995-03-01

T Maruo, K Murata, H Matsuo, T Samoto, M Mochizuki

{"title":"Insulin-like growth factor-I as a local regulator of proliferation and differentiated function of the human trophoblast in early pregnancy.","authors":"T Maruo, K Murata, H Matsuo, T Samoto, M Mochizuki","doi":"","DOIUrl":"","url":null,"abstract":"In order to elucidate the role of insulin-like growth factor-I (IGF-I) in human placental growth and function, the effects of IGF-I on the proliferation and differentiation of trophoblasts were investigated using an organ culture system of early placental tissues. Explants of trophoblastic tissues obtained from 4-5-week or 6-12-week placentae were, respectively, cultured with or without IGF-I, in a serum-free condition. The effect of IGF-I on the proliferative activity of trophoblasts was examined by immunocytochemical techniques with a monoclonal antibody to proliferating cell nuclear antigen (PCNA), while the effect of IGF-I on the differentiated function of trophoblasts was assessed by determining the ability to secrete human chorionic gonadotropin (hCG) and human placental lactogen (hPL). In 4-5-week placentae, IGF-I and IGF-I receptor were almost exclusively localized in cytotrophoblasts and IGF-I augmented the proliferative activity of cytotrophoblasts without affecting the ability to secrete hCG and hPL. By contrast, in 6-12-week placentae, IGF-I and IGF-I receptor were localized in both cytotrophoblasts and syncytiotrophoblasts and IGF-I stimulated the secretion of hCG and hPL following the enhancement of the proliferative activity of trophoblasts. In column chromatography of the serum-free medium obtained following 5-day culture of early placental tissues, an elution peak of immunoreactive IGF-I was found in the fractions similar to the elution region of [125I]IGF-I. These findings suggest that IGF-I acts as an autocrine/paracrine factor in regulating early placental growth and function.","PeriodicalId":11444,"journal":{"name":"Early pregnancy : biology and medicine : the official journal of the Society for the Investigation of Early Pregnancy","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1995-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20292561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0