Ecological genetics最新文献_第7页

Transplastomic plants — new approaches to solving “old” problems 移栽植物--解决 "老 "问题的新方法

Ecological genetics Pub Date : 2023-12-04 DOI: 10.17816/ecogen568520

Y. Sidorchuk, P. Belavin, A. Zagorskaya, Elena S. Khairulina, E. Deineko

{"title":"Transplastomic plants — new approaches to solving “old” problems","authors":"Y. Sidorchuk, P. Belavin, A. Zagorskaya, Elena S. Khairulina, E. Deineko","doi":"10.17816/ecogen568520","DOIUrl":"https://doi.org/10.17816/ecogen568520","url":null,"abstract":"Transplastomic plants are capable to accumulate the significant amounts (up to 70% of TSP) of target recombinant proteins in tissues. However, the production of such forms is severely limited by the low yield of initial transformants. This problem requires the development and optimization of new approaches to the delivery of transgenes into chloroplasts and an increase in the frequency of their integration into the plastome. Transplastomic tobacco plants expressing thegfpreporter gene and theaadAselectable marker under the control of thePrrnG10Lpromoter and theTpsbAterminator were obtained in the laboratory of plant bioengineering. It is known that the selected promoter and insertion region (between the tRNA genes of isoleucine and alanine) are capable to provide a high yield of recombinant proteins in the leaves of transplastomic plants [1]. However, the content of recombinant GFP in the leaves of the obtained transplastomic plants was determined at the level of 0.12%, and the variability for this trait was minimal and ranged from 0.09 to 0.16% of TSP. Insufficient accumulation of the target protein in transformants is not associated with transcription disorders or the presence of non-transgenic copies of the plastome. Probably, the low frequency of transformation and the lack of variability between the transformants are the reasons that make it difficult to select highly productive forms. It is proposed to increase the efficiency of targeted delivery of genetic constructs to plastids using single-walled carbon nanotubes loaded with recombinant DNA. This process can also be facilitated by our proposed approach to increase the frequency of DNA double-strand breaks in target regions of the plastome through the use of the CRISPR-Cas9 genome editing system. \u0000This work was supported by the Russian Science Foundation grant No. 23-24-00545.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"32 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138603796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Putative molecular pathways of autoregulation of nodulation activated by CLE peptides in pea 由 CLE 肽激活的豌豆拔节自调节的假定分子途径

Ecological genetics Pub Date : 2023-12-04 DOI: 10.17816/ecogen568446

Liliya A. Kochetkova, M. Lebedeva, Lyudmila A. Lutova

{"title":"Putative molecular pathways of autoregulation of nodulation activated by CLE peptides in pea","authors":"Liliya A. Kochetkova, M. Lebedeva, Lyudmila A. Lutova","doi":"10.17816/ecogen568446","DOIUrl":"https://doi.org/10.17816/ecogen568446","url":null,"abstract":"Legume plants are important for ecosystems due to their ability to form root nodules in symbiosis with rhizobia, where nitrogen fixation takes place. The number of symbiotic nodules is regulated by the CLE peptides inhibiting excessive nodule formation. Previously, we have identified four genes encoding CLE peptides, activated in response to rhizobia inoculation in pea. Three of them, PsCLE13, PsCLE12 and PsNIC-like, were also activated by nitrate, and, therefore, they could mediate nitrate-dependent inhibition of nodulation [1]. Overexpression of PsCLE13 and PsCLE12 inhibited nodulation on transgenic roots: however, the role of PsNIC-like and PsCLE12-like have not been investigated. \u0000In this study, we constructed vectors for overexpression of the PsCLE12-like and PsNIC-like genes to study their possible role in nodulation, and also analyzed the expression levels of nodulation-related genes in transgenic roots overexpressing four PsCLEs genes. Moreover, vectors for CRISPR-Cas9-mediated gene editing of the PsCLE12 and PsCLE13 genes were constructed to further explore the role of these genes in nodulation. Overexpression of PsCLE12-like, PsCLE13 and PsCLE12 resulted in increased expression levels of TOO MUCH LOVE (PsTMLs) genes known as root-acting regulators of nodule number. In addition, in the roots overexpressing four PsCLEs genes, down regulation of the PsSYM37 gene (encoding the receptor for Nod-factors) was observed, suggesting that the CLE peptides might inhibit the development of symbiotic nodules at the earliest stages of symbiosis development upon Nod-factor perception.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"2 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138603957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular screening of squash and patisson squash collection samples using markers of the Pm-0 gene, which controls resistance to powdery mildew 利用控制白粉病抗性的Pm-0基因标记对南瓜和西葫芦采集样本进行分子筛选

Ecological genetics Pub Date : 2023-08-16 DOI: 10.17816/ecogen110988

F. A. Berensen, Tatiana M. Piskunova, S. V. Kuzmin, Andrey F. Moskalu, O. Antonova, A. Artemyeva

{"title":"Molecular screening of squash and patisson squash collection samples using markers of the Pm-0 gene, which controls resistance to powdery mildew","authors":"F. A. Berensen, Tatiana M. Piskunova, S. V. Kuzmin, Andrey F. Moskalu, O. Antonova, A. Artemyeva","doi":"10.17816/ecogen110988","DOIUrl":"https://doi.org/10.17816/ecogen110988","url":null,"abstract":"Powdery mildew (PM) is one of the most harmful diseases of cucurbits. Modern commercial varieties of squash Cucurbita pepo L. var. giraumonas Duch and patisson C. pepo var. melopepo L. received powdery mildew resistance genes from wild species. The Pm-0 resistance gene belongs to the linkage group 10; two CAPS markers were developed for its mapping [15]. The main intragenic marker NBS_S9_1495924/HaeIII is localized in the NBS-LRR region, the additional marker S9_1539675/MspI shows complete co-segregation with resistance to PM [15]. In the present study, these markers were used for molecular screening of an experimental set of squash and patisson samples (differ in resistance to powdery mildew) from the VIR gene bank collection and perspective breeding lines of the Krymsk Experiment Breeding Station branch of VIR. In total, 80 samples were investigated. Samples, carrying fragments of both CAPS markers (17) and fragments of only one marker (31) were found. Presence of two markers of the Pm-0 gene (NBS_S9_1495924/HaeIII и S9_1539675/MspI) has the strong correlation with resistance to PM (r = 0.837). \u0000For three powdery mildew-resistant samples, which has demonstrated presence of both markers of the Pm-0 gene, additional analysis of individual plants was done. Resistant plants with Pm-0 gene markers were self-pollinated to create resistant lines. Thus, molecular screening allowed to preserve the valuable trait of resistance during maintaining of the squash collection samples.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76714508","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophytic microorganisms of potato (Solanum tuberosum L.): biodiversity, functions and biotechnological potential 马铃薯内生微生物:生物多样性、功能和生物技术潜力

Ecological genetics Pub Date : 2023-08-16 DOI: 10.17816/ecogen196737

N. Karamova, Ammar A. Tuama, Z. Stasevski

引用次数: 0

Stanislava I. Narbut, the author of the first radish genetic collection in Russia Stanislava I. Narbut是俄罗斯第一个萝卜基因收集的作者

Ecological genetics Pub Date : 2023-08-16 DOI: 10.17816/ecogen322833

Ksenia A. Kuznetsova, Elena P. Efremova, I. S. Buzovkina, I. Dodueva, L. Lutova

引用次数: 0

The diversity of genes for the synthesis of opines and their products in representatives of various taxa 不同分类群中代表植物合成碱及其产物的基因多样性

Ecological genetics Pub Date : 2023-08-16 DOI: 10.17816/ecogen492282

Anton D. Shaposhnikov, T. Matveeva

引用次数: 1

Variability in the productivity of peanut accessions (Arachis hypogaea L.) at ecological-geographical testing 花生品种(arachhis hypogaea L.)生产力在生态-地理试验中的变异

Ecological genetics Pub Date : 2023-08-16 DOI: 10.17816/ecogen340801

Viktoriya D. Bemova, T. Yakusheva, M. Asfandiyarova, V. Gavrilova, N. V. Kishlyan, L. Novikova

{"title":"Variability in the productivity of peanut accessions (Arachis hypogaea L.) at ecological-geographical testing","authors":"Viktoriya D. Bemova, T. Yakusheva, M. Asfandiyarova, V. Gavrilova, N. V. Kishlyan, L. Novikova","doi":"10.17816/ecogen340801","DOIUrl":"https://doi.org/10.17816/ecogen340801","url":null,"abstract":"BACKGROUND: Russia is one of the largest peanut-buying countries. At the same time, in the south of the country, a number of zones meet the requirements for the cultivation of peanuts. \u0000AIM: Identification of a new source material for peanut breeding by the method of ecological and geographical testing of collection samples. \u0000MATERIALS AND METHODS: The work used 30 of peanuts accessions from the VIR collection of various origins. To assess the stability of productivity, standard deviation (s), coefficient of variation (Cv) and regression (i) for environmental conditions according to Eberhart and Russell were used. \u0000RESULTS: As a result of the study, the possibility of growing individual varieties of peanuts in the south of the RF under modern conditions was confirmed. It was determined that some samples are more productive and suitable as starting material for the conditions of the Krasnodar Territory (k-283, k-1157), others for the conditions of the Astrakhan region (k-317, k-868). The accessions of the VIR collection were found to be more productive at 2 points experience, also marked as plasticity k-751, k-283, k-626, k-1533, k-1987. \u0000CONCLUSIONS: In contrasting conditions (two geographical points for 3 years of study), peanuts accessions were identified that strongly react to changes in environmental conditions. Stable and plastic in productivity accessions can serve as the initial breeding material. It has been established that peanuts can be cultivated in the south of the Russian Federation, namely in the Astrakhan Region and the Krasnodar Territory.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75142922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inositol phosphates’ synthesis in pea Pisum sativum L. root seedlings at the early stages after Rhizobium leguminosarum bv. viciae inoculation 豆科根瘤菌侵染后早期豌豆幼苗肌醇磷酸合成的研究。viciae接种

Ecological genetics Pub Date : 2023-05-12 DOI: 10.17816/ecogen117412

A. D. Bovin, Svetlana A. Shirobokova, G. Karakashev, E. Dolgikh

{"title":"Inositol phosphates’ synthesis in pea Pisum sativum L. root seedlings at the early stages after Rhizobium leguminosarum bv. viciae inoculation","authors":"A. D. Bovin, Svetlana A. Shirobokova, G. Karakashev, E. Dolgikh","doi":"10.17816/ecogen117412","DOIUrl":"https://doi.org/10.17816/ecogen117412","url":null,"abstract":"Studying the role of inositol phosphates in the regulation of signal exchange between leguminous plants and nodule bacteria is of great interest since it affects the regulation of calcium level in the root cells in response to bacterial signals during symbiosis development. The regulation of intracellular calcium content is one of the key events in the control of symbiosis development, but remains very poorly understood. In present work, we revealed a significant increase in the content of inositol hexasphosphate (IP6), which occurs in response to the recognition of Nod factors and indicates that in plants, unlike animals, this form (along with the inositol triphosphate (IP3)) may be important for signal transduction. This is consistent with the data that receptor for IP3 in plants has not yet been found, despite numerous efforts. \u0000Expression analysis of the genes encoding enzymes of two biosynthetic pathways for inositol phosphates showed stimulation of the PsITPK1 gene (Psat6g210960), which can control the phospholipid-independent pathway for synthesis of these compounds. Despite the fact that PsPIP5K (Psat5g134320) important for another pathway did not show increased expression in our experiments upon inoculation, the activation of the phospholipid-dependent pathway of inositol phosphate biosynthesis can be evidenced by stimulation of a number of genes encoding pospholipases C (PLCs) which were previously found in pea Pisum sativum as well as during analysis of transcriptome of Medicago truncatula root treated with Nod factors. Therefore, in plants, in contrast to animals, the pathways for the synthesis of inositol phosphates can be more diverse, which indicates the plasticity of signal pathways.","PeriodicalId":11431,"journal":{"name":"Ecological genetics","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78399650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review of population history reconstruction methods in conservation biology 保护生物学种群历史重建方法综述

Ecological genetics Pub Date : 2023-05-12 DOI: 10.17816/ecogen120078

A. Totikov, Andrey A. Tomarovsky, Aliya R. Yakupova, A. Graphodatsky, S. Kliver

引用次数: 0

Terry buttercup wild flowers (Ranunculus acris) variability in school ecology education: ecocentrism formation 金凤花在学校生态教育中的变异性:生态中心主义的形成

Ecological genetics Pub Date : 2023-05-12 DOI: 10.17816/ecogen197559

E. Daev

引用次数: 0