I.P. Pavlov Russian Medical Biological Herald最新文献

{"title":"Tactics of Endovascular Treatment of Patients with Coronary Heart Disease with Recurrent Coronary In-Stent Restenosis Using Second- and Third-Generation Stent Systems and Paclitaxel-Coated Balloon Catheters","authors":"Yuriy L. Shevchenko, D. Ermakov, Mikhail A. Maslennikov, Daniil S. Ul'bashev, Anastasiya Yu. Vakhrameyeva","doi":"10.17816/pavlovj625996","DOIUrl":"https://doi.org/10.17816/pavlovj625996","url":null,"abstract":"INTRODUCTION: The binary in-stent restenosis (ISR) still remains the main factor limiting the effectiveness of percutaneous coronary intervention in the long-term period. Histologically, ISR is defined as neointimal hyperplasia leading to hemodynamically significant narrowing of the arterial lumen. Patients with coronary artery (CA) restenosis represent a particularly challenging group for endovascular treatment. \u0000AIM: To compare effectiveness and safety of the endovascular correction of coronary in-stent restenosis using second- and third-generation stent systems and balloon angioplasty with a drug-coated balloon catheter. \u0000MATERIALS AND METHODS: The study retrospectively included 62 patients with recurrent ISR after the previous endovascular correction. The patients underwent treatment with re-stenting in Saint George Clinic of Thoracic and Cardiovascular Surgery of the National Pirogov Medical Surgical Center in 2016–2023 with use of second- and third-generation drug-eluting stents — cobalt (cobalt alloy) systems with zotarolimus, cobalt-chromium stent systems with sirolimus and zotarolimus, platinum-chromium stent systems with everolimus with biodegradable drug coating. Balloon angioplasty was performed using paclitaxel-coated balloon catheters. The primary endpoint of the study was the target lesion failure (TLF) of CA. The secondary endpoint was major adverse cardiovascular events (MACE). \u0000RESULTS: The TLF rate was 15.6% vs. 13.3% and 28.1% vs. 46.7% in the groups with use of a drug-eluting stent and balloon angioplasty at 1- and 2-year follow-up, respectively (p = 0.30). MACE was recorded in 18.8% vs. 16.7% and 37.5% vs. 56.7% of cases in the groups with use of a drug-eluting stent and balloon angioplasty at 1- and 2-year follow-up, respectively (p = 0.25). The dispersion analysis of predictors of TFL risks identified three factors showing a reliable correlation with the probability for TFL by the second follow-up year in both groups: (1) recurrence of binary ISR (hazard ratio (HR) 2.21; 95% confidence interval (CI) 0.95–4.01; p = 0.03)) in 365 days after the third stage of the percutaneous coronary intervention; (2) length of coronary restenotic lesion (per every 10 mm) (HR 1.25; 95% CI 0.99–1.40; p = 0.002); (3) occlusive restenosis (HR 4.16; 95% CI 0.43–26.96; p = 0.04). \u0000CONCLUSIONS: The implantation of a second- and third-generation drug-eluting stent and balloon angioplasty with use of a drug-coated catheter are comparable in the effectiveness and safety in correcting the recurrent ISR, however, restenting is associated with a lower probability for developing TFL and adverse events.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"6 32","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140732858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of Physical and Motor Development of Children with Congenital Heart Diseases","authors":"E.M. Savova, A. Zavarina, V. Shvedunova, Marina L. Ermolenko","doi":"10.17816/pavlovj322794","DOIUrl":"https://doi.org/10.17816/pavlovj322794","url":null,"abstract":"INTRODUCTION: Children with congenital heart diseases (CHD) have certain peculiarities of development determined by the pathology of the cardiovascular system. The type, severity of CHD, the age of correction of the disorder and a number of other factors have a direct impact on the severity of its non-cardiologic complications, such as disorders in physical and motor development attributable to specific conditions of formation of the nervous system in children with CHD. \u0000AIM: To systematize the currently available data on the peculiarities of physical and motor development of children with CHD and their underlying factors, to justify the importance of a more in-depth examination of children at all stages of their development and of the need to correct the identified disorders starting from the moment of their diagnosis. \u0000The review presents the data on the incidence and peculiarities of deviations in motor development of children with CHD at different age periods; describes factors predisposing to their development, as well as the possibilities of diagnosis and correction of such disorders. Besides, information is provided on the development of the balance function that is also impaired in this cohort of patients. Since the physical and motor development of children are closely interrelated and related to the development of the nervous and muscular systems, and the muscle strength and balance are integral components of some motor skills, each of these parameters is considered in a separate section of the article. Acyanotic heart diseases are more commonly associated with disorders in the weight gain, and cyanotic ones — with retardation of children in growth and body mass. Another manifestation of retardation of physical development of children with CHD is deficit of muscle mass development reflected in the research-confirmed reduction of muscle strength in this category of patients. Disorders in fine and/or large motor skills are noted in one to two thirds of children with CHD from an early age to adulthood. Disorders in the motor sphere in childhood entail physical inactivity, psychological and cognitive problems, difficulties in socialization significantly reducing the quality of life. \u0000CONCLUSION: Children with CHD are characterized by peculiarities of the physical and motor development, which have a significant impact on the child’s life. Therefore, currently of paramount importance is not only examination of the cardiovascular system of children with CHD, but also their physical and motor development, as well as timely identification of deviations in the physical and motor development and their targeted rehabilitation.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140733148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atypical Forms of Lower Limb Varicose Vein Disease: Features of Diagnosis and Surgical Treatment","authors":"I. N. Shanayev, V. S. Korbut, R. M. Khashumov","doi":"10.17816/pavlovj107079","DOIUrl":"https://doi.org/10.17816/pavlovj107079","url":null,"abstract":"INTRODUCTION: Lower limb varicose vein disease (LLVVD) is the most common vascular disease with a predominant lesion of the main trunks of saphenous veins. At the same time, there exist atypical variants of lesion of the venous system in LLVVD, which cause difficulties in diagnosis and treatment. \u0000AIM: To study the incidence rate, anatomical bases of the varicose transformation, the features of reflux formation and the results of surgical treatment in atypical forms of LLVVD. \u0000MATERIALS AND METHODS: The study involved 600 patients with LLVVD, C2-C3 class of clinical manifestations in CEAP classification; 82 of them had atypical forms. The mean age of patients was 40.2 ± 9.2 years, duration of disease 15.0 ± 5.6 years. Duplex scanning of the lower limb venous system was conducted according to Russian recommendations for the diagnosis and treatment of chronic venous disorders of lower limbs of 2018. Patients with atypical forms of LLVVD additionally underwent computed tomography of the venous system with contrast. Surgical treatment of 50 patients with atypical forms of LLVVD included separation of the site of opening of a tributary in the area of saphenofemoral junction after preliminary marking and isolated elimination of varicose tributaries using Muller hooks; incompetent perforating veins were ligated at the epi- or subfascial levels depending on the location. The results were considered in the periods for up to two years. \u0000RESULTS: According to our data, the incidence of atypical forms of LLVVD was 13.7%. Lesion of the large tributaries of the main saphenous veins accounted for the highest proportion of atypical forms of LLVVD — 68.3%. Of these, varicose transformation of the anterolateral tributary made 98.2%, and of the superficial iliac circumflex vein — 1.8%. Isolated varicose transformation of perforating veins occurred in 31.7% of cases, where transformation of perforating veins of the gluteal area made 7.7%, of perforating veins of the posterolateral surface of the thigh — 46.2%, and of perforating veins of the patella region — 46.2%. The technical success in the postoperative period in the form of elimination of varicose saphenous veins and of the source of their incompetence was achieved in 100% of cases. \u0000CONCLUSIONS: The incidence of atypical forms of LLVVD is 13.7%, with the main trunks of saphenous veins remaining competent. The anatomical and hemodynamic basis for such forms of LLVVD is incompetence of the deep vein valves, from where the reflux is transmitted to tributaries of the saphenofemoral junction and/or perforating veins of the gluteal region, femoral region or popliteal fossa. Precise separation of varicose tributaries and perforating veins with preservation of the main trunks of subcutaneous veins is an organ-saving method of LLVVD treatment with a good effect in the follow-up period of up to two years.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"21 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139383176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Physical Activity on Portal Vein Resistance Index","authors":"A. A. Vinogradov, Irina V. Andreyeva, Evgeniya S. Simakova","doi":"10.17816/pavlovj109095","DOIUrl":"https://doi.org/10.17816/pavlovj109095","url":null,"abstract":"INTRODUCTION: In norm, adaptation of an organism to physical exercise is determined by the liver functions that ensure high working capacity. To this end, of a special interest are parameters of portal hemodynamics, in particular, the resistance index (RI) as the main coordinator of the differences in the linear velocity of blood flow between the phases of cardiac cycle. Dosed physical exercise is a relevant way of health improvement for not only practically healthy people, but also for people who have suffered COronaVIrus Disease 2019 (COVID-19), and who were diagnosed with liver damage. Such patients are prescribed physical rehabilitation for their health recovery. \u0000AIM: To study age-related changes in the portal vein (PV) RI and the effect of physical exercise in the form of forced dosed running on this parameter of the portal hemodynamics in an experiment on rats. \u0000MATERIALS AND METHODS: In young rats of 14–16-month age, which corresponds to 35–40 years of human age, the age-related changes in the portal vein RI were studied using ultrasound Dopplerometry. In animals of the control group, PV RI was studied during 75-day observation, in the experimental group — in the course of daily forced, dosed running on the 1st day, after 15, 30, 45 and 60 days, and also in 15 days after cessation of physical exercise. The actual effect of physical exercise on the change in the PV RI was evaluated with the deduction of age-related changes of the parameter. \u0000RESULTS: In the animals of the control group, after 60 days of the experiment, the PV RI increased by 5.44 ± 1.22%, and in the experimental group — by 24.10 ± 2.12%. After a 15-day recovery period, the parameter declined, but remained 9.11 ± 0.70% above the initial level. After deduction of the age-related changes of the parameter, the PV RI was 4.36 ± 1.93% above the initial one. \u0000CONCLUSION: Changes in the PV RI in daily dosed physical exercise did not go beyond the physiological norm and were transient. After a 15-day recovery period, the PV RI practically approached the initial parameter.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"60 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139381840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The State of Colon Microbiocenosis and Antioxidant Properties of Rat Colonocytes in Environmental Dysbiosis and Use of Probiotic Bifidumbacterin® and Symbiotic Acipol®","authors":"V. A. Korolev, O. A. Medvedeva, Vera A. Ryadnova, Alina V. Shevchenko, Ivan V. Korolev, Egor V. Korolev","doi":"10.17816/pavlovj121795","DOIUrl":"https://doi.org/10.17816/pavlovj121795","url":null,"abstract":"INTRODUCTION: The microbial community of the colon is a single microecological system which quickly responds to the exposure to external and internal factors with quantitative and qualitative changes with the result of development of the condition termed dysbiosis. The changes in the composition of colon microbiocenosis can lead to a number of diseases and to the development of immunopathological and allergic conditions. \u0000AIM: To evaluate the state of the colon microbiocenosis and antioxidant properties of rats’ colonocytes against a background environmental dysbiosis and the use of probiotic Bifidumbacterin® and symbiotic Acipol®. \u0000MATERIALS AND METHODS: In the experiment, intoxication in rats was modeled with the fungicide Thiram® (dimethylcarbamothioylsulfanyl N,N-dimethylcarbamodithioate, 1.6 mg per animal of 200 g mass) for 28 days, which led to the development of environmental dysbiosis. The detected changes were subsequently corrected within 21 days with the probiotic Bifidumbacterin® (0.14 ml per animal of 200 g mass) and the symbiotic Acipol® (0.08 ml per animal of 200 g mass). The composition of the microflora of colon mucosa of rats was studied using a bacteriological method. The activity of antioxidant protection enzymes of colonocytes was studied by the content of superoxide dismutase and catalase. The condition of lipid peroxidation processes was assessed by the content of diene conjugates and malondialdehyde. \u0000RESULTS: In introduction of Thiram®, reduction of the amount of obligate and opportunistic representatives of colon microbiocenosis was noted, as well as intensification of lipid peroxidation processes in the conditions of weakening of antioxidant protection in colonocytes. Application of Bifidumbacterin® and Acipol® resulted in increase in the amount of obligate bacteria and decrease in the content of opportunists of the colon microbiota with the strongest effect of Acipol® on the values of the determined parameters. When studying changes in biochemical parameters in animal colonocytes, both drugs were found to contribute to the normalization of the content of malondialdehyde in the colon tissue, besides, Acipol® led to a decrease in the concentration of diene conjugates in colonocytes. \u0000CONCLUSION: Introduction of Thiram® in all the specified periods affected the qualitative and quantitative composition of the microbiota and biochemical parameters in the colonocytes of the animals. The use of Bifidumbacterin® and Acipol® preparations resulted in the restoration of the qualitative and quantitative composition of colonic microorganisms, and also had a positive effect on the content of lipid peroxidation products in colonocytes, but more efficient was found to be Acipol® symbiotic.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"5 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139382832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Different Treatment Tactics of Superficial Thrombophlebitis of Lower Limbs (with Development of Mathematical Model for Prediction of Therapeutic Effectiveness)","authors":"R. Y. Kalinin, I. Suchkov, M. Uporov, Anastasiya N. Taraskina, D. S. Titov, M. Y. Klishchenko","doi":"10.17816/pavlovj192535","DOIUrl":"https://doi.org/10.17816/pavlovj192535","url":null,"abstract":"INTRODUCTION: The main goals of treatment of superficial thrombophlebitis of lower limbs (ST LL) can be achieved using different treatment tactics: conservative treatment, surgical intervention, and their combination. \u0000AIM: To perform a comparative analysis of the effectiveness of pharmacotherapy, crossectomy in combination with pharmacotherapy and of phlebectomy in combination with pharmacotherapy in patients with ST LL. \u0000MATERIALS AND METHODS: A comparative analysis of the effectiveness of the therapeutic tactics of ST LL was conducted (86 patients; 36 men and 50 women). Group 1 patients received only conservative treatment, group 2 patients underwent crossectomy and conservative treatment in the postoperative period, group 3 — phlebectomy in combination with conservative treatment. The clinical effectiveness of treatment was evaluated by the recurrence rate and/or progression of the disease within 3 months after treatment and the level of life quality (Chronic Venous Insufficiency Questionnaire, CIVIQ 20, visual analog scale, VAS). Based on the data obtained in the study, a mathematical model was developed to determine the maximally effective treatment method. For mathematical modeling, a ‘random forest’ method was used. \u0000RESULTS: All the studied treatment methods demonstrated a comparative clinical effectiveness. Analysis of the dynamics of the studied parameters (adjusted for gender and age of the patients) compared with their initial values within each group showed that statistically significant changes in the psychological factor were observed in the pharmacotherapy group already on the 7th control day (p = 0.024), while in the crossectomy and phlebectomy groups only on the 14th day. In the groups of pharmacotherapy (p = 0.001) and phlebectomy (p = 0.005), the improvement in terms of the social factor occurred faster than in the group of crossectomy, since statistically significant differences were found on the 7th day, and in the group of crossectomy only on the 14th day. \u0000CONCLUSION: All the groups demonstrated comparable clinical effectiveness in normalization of the quality of life and the recurrence rate and/or progression of the disease within three months after completion of treatment. On the basis of the data obtained, predictive models have been constructed that allow, based on the initial characteristics of the patient, to determine the tactics of therapy that can ensure maximum effectiveness in terms of normalization of values, reflecting the quality of life and VAS parameter.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"68 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139381502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Culture Method and Regulation Mechanisms of Stages of C2C12 Cell Line Myogenesis","authors":"Mariya O. Isayeva, Fidan Т. Gadzhiyeva, Y. Abalenikhina, A. V. Shchulkin, E. Yakusheva","doi":"10.17816/pavlovj375362","DOIUrl":"https://doi.org/10.17816/pavlovj375362","url":null,"abstract":"INTRODUCTION: C2C12 is an immortalized mouse myoblast cell line actively used as in vitro experimental models to study skeletal muscle metabolism in biomedical research. C2C12 cells can differentiate into myocytes under appropriate culture conditions. It is important to understand the specifics of each stage of myogenesis and its regulatory mechanisms for the targeted exposure and development of medical drugs, taking into account the mechanisms of pathogenesis of various diseases. \u0000AIM: To describe the method of culturing С2С12 cell line and to study regulatory mechanisms of С2С12 cell line myogenesis. \u0000MATERIALS AND METHODS: The study was conducted on C2C12 cell line. Differentiation of myoblasts was induced in the nutrient medium containing 2% horse serum. The differentiation stages were evaluated by studying the cells on the 1st, 4th and 7th days. Cells before differentiation were used as a comparison group. At each differentiation stage, the myoblast fusion index (IM) was evaluated with preliminary staining of the cells by Romanowsky–Giemsa method. The cell differentiation mechanism was evaluated by the level of myosin, a-actin, myogenic differentiation protein (MyoD), myogenin (MyoG) using Western blot method. \u0000RESULTS: On the 1st day of C2C12 cells culturing, formation of myotubes was observed (IM = 0.15 ± 0.05), on the 4th day — fusion of myoblasts with the formation of binucleated cells accompanied by an increase in the amount of MyoD and α-actin protein (IM = 0.44 ± 0.14). By the 7th day of differentiation, the fusion of cells increased with the formation of myotubes containing more than two nuclei; the content of MyoD and α-actin did not differ from the control, and the amount of MyoG and myosin increased (IM = 0.77 ± 0.04). \u0000CONCLUSION: The described method of culturing C2C12 cell line in Dulbecco’s Modified Eagle’s Medium with a high glucose content (4500 mg/l), containing 2% horse serum, L-glutamine (4 mМ), 100 Un/ml and 100 µg/ml of penicillin and streptomycin, is suitable for the formation of muscle cells, where MyoD and MyoG participate in the regulation of the increase in the amount of specific muscle proteins — α-actin and myosin.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"17 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139383020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser Therapy in Comprehensive Treatment of Patients with Lymphedema of Lower Limbs","authors":"G. V. Yarovenko, S. Katorkin","doi":"10.17816/pavlovj217680","DOIUrl":"https://doi.org/10.17816/pavlovj217680","url":null,"abstract":"INTRODUCTION: Methods of physiotherapy using intravenous laser irradiation of blood (ILIB) that affects various pathogenetic factors of lymphedema, permits to achieve the required therapeutic effect and shows its promising perspectives. \u0000AIM: Evaluation of the effectiveness of ILIB to reduce the lymphedema volume in the lower limbs. \u0000MATERIALS AND METHODS: In 2020–2022, a comprehensive conservative treatment including compression therapy, pharmacotherapy and ILIB, was conducted in 60 patients of the mean age 45.3 ± 1.6 years with I–III stage lymphedema. Group 1 included patients with primary (n = 8) and group 2 (n = 52) with secondary lymphedema of the lower limbs. Laser radiation at 632 nm wavelength was used with standardized parameters: power 3 to 15 mW, pulse rate 80 to 2,000 Hz, exposure time 5 to 15 min, with a course (up to 10 sessions), daily, or interval (every 2nd day) load. Lymph outflow, microcirculation, soft tissues of the affected limb were evaluated using electrocoagulography, ultrasound examination of inguinal lymph nodes and soft tissues, triplex scanning, thermal imaging and McClure-Aldrich test. \u0000RESULTS: At the end of the treatment course, a reduction of the limb perimeter at the level of the middle third of the lower leg by 14.8 ± 0.7% was noted (from 48.7 ± 5.3 cm to 41.4 ± 0.9 cm). After 4 sessions and a treatment course, electrocoagulography revealed hypocoagulation with the formation of a loose clot with early retraction. Spearman test showed a direct relationship between changes in the fibrinolytic activity of blood and the perimeter of the lower leg in patients of group 2 with I–II stage of the disease (p 0.005). Thermography of the lower limbs showed increased IR radiation with a statistically significant increase (p 0.001) in the area of hyperthermia in the distal parts of the lower legs and the inner surface of the thighs. The results of McClure-Aldrich test showed a statistically significant slowdown of a skin papule resolution in the distal part of the lower legs from 27.13 ± 2.77 min to 35.72 ± 3.11 min (р 0.05) after ILIB in I stage of the disease. \u0000CONCLUSION: ILIB affects the internal factors of lymph outflow and contributes to its optimization in the comprehensive treatment of patients in the initial stage of secondary lymphedema of the lower limbs.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"31 14","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139383802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of ABCB1 Protein Function in the Cerebral Cortex with the Underlying Global Cerebral Ischemia","authors":"I. V. Chernykh, A. V. Shchulkin, Natal'ya M. Popova, M. V. Gatsanoga, E. Yakusheva","doi":"10.17816/pavlovj111932","DOIUrl":"https://doi.org/10.17816/pavlovj111932","url":null,"abstract":"INTRODUCTION: ABCB1 is a membrane transporter protein responsible for efflux of a wide range of drugs from cells. The study of the mechanisms of regulation of the functioning of ABCB1 protein in the brain in its ischemia will permit to propose new approaches to pharmacotherapy of cerebral ischemic pathology. \u0000AIM: To study the regulation of ABCB1 protein function in the cerebral cortex of rats with global cerebral ischemia. \u0000MATERIALS AND METHODS: The experiment was performed on 30 male rats with global cerebral ischemia modeled by bilateral occlusion of the common carotid arteries. The amount of ABCB1 protein and Nrf2 and HIF-1α transcription factors in the cerebral cortex was determined by enzyme immunoassay. The free radical status of the cerebral cortex was assessed by the concentration of malondialdehyde, SH groups, and by glutathione peroxidase (G-per) activity. \u0000RESULTS: Bilateral occlusion of the common carotid arteries caused an increase in the level of ABCB1 protein in the cerebral cortex of rats by the 4th hour of ischemia; in 24 hours it remained elevated, and in 72 hours decreased to values that did not differ from those of falsely operated rats. The content of malondialdehyde in the cerebral cortex increased in 2 and 4 hours after occlusion and then gradually decreased to the initial values. In 30 minutes and 4 hours after ischemia modeling, G-per activity decreased compared to the control values. The content of Nrf2 in the cerebral cortex increased in 2 and 4 hours after occlusion, then slightly decreased on the next day, and reached the initial values on the 3rd day of the experiment. The amount of HIF-1α increased only in 24 and 72 hours after the surgery. \u0000CONCLUSION: The amount of ABCB1 protein in the cerebral cortex of rats with global cerebral ischemia depends on the severity of oxidative stress, with Nrf2 and HIF-1α transcription factors playing a role in its regulation. Reduction of the amount of the transporter in the blood-brain barrier through the influence on the lipid peroxidation processes or synthesis of the studied transcription factors expands the possibilities of using ABCB1 protein substrates for improving the effectiveness of pharmacotherapy of diseases of the central nervous system.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"29 36","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139382544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurophysiological Factors Associated with Compression and Reflex Mechanisms of Lower Back Pain","authors":"I.A. Barinova, L. N. Erkhova, V. A. Zhadnov, R. Zorin, Nadezhda N. Kiryukhina, Andrey A. Kosolapov, G. A. Leonov, Aleksandr O. Burshinov","doi":"10.17816/pavlovj303663","DOIUrl":"https://doi.org/10.17816/pavlovj303663","url":null,"abstract":"Introduction: Vertebrogenic pain syndromes (PSs) in the lower back (LB) are among the most common neurological disorders in the population. Currently, clinical, neurophysiological and neuroimaging characteristics are used as predictors of the course and effectiveness of the treatment for vertebrogenic PSs in the LB. \u0000Aim: To identify the neurophysiological factors associated with compression and reflex mechanisms of PSs in the LB for their differentiation in complicated diagnostic cases. \u0000Materials and methods: The study included 73 patients, 43 of them men and 30 women of the mean age 46.5 years. Based on the results of clinical neurological examination and the magnetic resonance imaging data, reflex vertebrogenic PSs (lumboischialgia) were verified in 40 cases, and radiculopathy of L5 and/or S1 (compression vertebrogenic PSs) in 33 cases. The patients underwent stimulation electroneuromyography with record of motor and sensory responses from the lower limbs, of F- and H-response, and examination of heart rate variability. \u0000Results: Logit regression analysis was used to create models permitting to divide patients into groups with different mechanisms of PSs based on the neurophysiological parameters. The first model demonstrated high sensitivity in the identification of predominantly compression vertebrogenic disorders with the independent factors being the mean F-wave latency and the sensory response amplitude. The second model was more sensitive in identification of reflex PSs with the main predictors being the F-response amplitude and heart rate variability parameters. \u0000Conclusion: The identified factors reflect the role of conduction disorders in compression PSs, as well as the involvement of segmental motor neuronal apparatus and autonomic regulatory mechanisms in the reaction in reflex PSs, which are additional sources of information in taking medical decisions in complicated diagnostic cases.","PeriodicalId":113364,"journal":{"name":"I.P. Pavlov Russian Medical Biological Herald","volume":"29 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139382570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}