Drug Delivery System最新文献

{"title":"Lipid nanoparticles for mRNA delivery","authors":"Hiroki Tanaka, Y. Sakurai, H. Akita","doi":"10.2745/dds.37.237","DOIUrl":"https://doi.org/10.2745/dds.37.237","url":null,"abstract":"","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46643833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mRNA therapeutics for central nervous system disorders","authors":"Yuta Fukushima, K. Itaka","doi":"10.2745/dds.37.247","DOIUrl":"https://doi.org/10.2745/dds.37.247","url":null,"abstract":"The high efficacy of mRNA COVID-19 vaccine encourages the wider application of mRNA therapeutics. Protein replacement therapy with mRNA therapeutics is a promising alternative DDS approach for administering trophic factor proteins in central nervous system disorders or enzyme replacement therapy in inherited enzyme deficient diseases. Although the concept to deliver the mRNA in vivo as a drug was demonstrated as early as 1990, mRNA instability hindered subsequent research development. The polymer-based carrier, polyplex nanomicelle, is a novel carrier for in vivo mRNA administration. Here, we introduce the researches of in vivo mRNA administration using the nanomicelle carrier to treat animal models, especially focusing on the central nervous system disorders including Alzheimer’s disease, spinal cord injury and ischemic brain disease. We discuss the advantages of mRNA therapeutics and the characteristics of diseases which are highly suitable for mRNA therapeutics. © 2022, Japan Society of Drug Delivery System. All rights reserved.","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45047070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and purification of exosomes","authors":"I. Nakase, Yuki Takahashi","doi":"10.2745/dds.37.264","DOIUrl":"https://doi.org/10.2745/dds.37.264","url":null,"abstract":"","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42868577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-responsive RNA delivery","authors":"Kazunori Watanabe, T. Ohtsuki","doi":"10.2745/dds.37.229","DOIUrl":"https://doi.org/10.2745/dds.37.229","url":null,"abstract":"","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44551534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cationic polypeptide design for polyion complex-mediated mRNA delivery","authors":"Mitsuru Naito, K. Miyata","doi":"10.2745/dds.37.221","DOIUrl":"https://doi.org/10.2745/dds.37.221","url":null,"abstract":"Recently, with the success of the COVID-19 vaccine, mRNA therapeutics have received a great deal of attention as a next-generation biopharmaceutical. One of the current key issues in mRNA therapeutics is the development of delivery vehicles with higher safety and targetability, excepting the liver. Herein, we introduce a systematic design strategy of cationic polypeptides and their polyplexes for enhanced mRNA delivery. Indeed, a series of cationic polypeptides were synthesized by the aminolysis reaction of poly（β-benzyl-L-aspartate）with varying amine compounds. First, cationic polyaspartamide derivatives were developed for efficient endosomal escape（or endosomal membrane destabilization）by highlighting the acidic pH-sensitivity of aminoethylene（-NHCH2 CH2-）units. Notably, a polyaspartamide derivative（PAsp（DET）） bearing diethylenetriamine（DET）moieties allowed efficient endosomal escape of polyplexes with lowered cytotoxicity. Second, varying hydrophobic moieties were introduced into the side chains of polyaspartamide derivative with the DET moieties to enhance the stability of mRNA-loaded polyplexes. The results indicated that the derivatives with a hydrophobicity index（or logD） ＞ –2.4 elicited efficient mRNA transfection in cultured cells. As a result, a polyaspartamide derivative（PAsp（DET/CHE））with DET and cyclohexylethyl（CHE）moieties achieved the most efficient mRNA transfection without marked cytotoxicity, allowing the topical mRNA delivery in the ventricle via intracranial/intrathecal administration and the systemic mRNA delivery into the lung via intravenous administration. Third, self-catalytic degradation of cationic polyaspartamide derivatives was investigated by slightly changing the spacer length in the side chains. The main-chain degradation was substantially affected by the spacer length；the loss of one methylene spacer resulted in the 5-fold higher degradation rate. A polyaspartamide derivative（PAsp（EDA）） bearing the shorter spacer showed higher mRNA transfection efficiency in cultured cells with reduced cytotoxicity with an increase in pre-incubation time, compared with those bearing the longer spacers. Altogether, it is demonstrated that the polyplex-mediated mRNA delivery can be dramatically improved by fine-tuning the chemical structure of cationic polypeptides. © 2022, Japan Society of Drug Delivery System. All rights reserved.","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41610577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Think ahead and prepare for future needs","authors":"Takao Inoue","doi":"10.2745/dds.37.193","DOIUrl":"https://doi.org/10.2745/dds.37.193","url":null,"abstract":"","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42529556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 37th Annual Meeting of the Academy of Pharmaceutical Science and Technology, Japan","authors":"Masami Ukawa","doi":"10.2745/dds.37.272","DOIUrl":"https://doi.org/10.2745/dds.37.272","url":null,"abstract":"","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45838882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The current situation and perspectives of mRNA delivery to the kidney","authors":"M. Kawaguchi, Naoya Kato, M. Kamiya, Hidefumi Mukai, Shigeru Kawakami","doi":"10.2745/dds.37.253","DOIUrl":"https://doi.org/10.2745/dds.37.253","url":null,"abstract":"","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42532825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design of Synthetic mRNAs for Highly Efficient Translation","authors":"Masahito Inagaki, Mizuki Tada, H. Abe","doi":"10.2745/dds.37.196","DOIUrl":"https://doi.org/10.2745/dds.37.196","url":null,"abstract":"Recently messenger RNA （mRNA）therapeutics is received much attention as one of the vaccination therapies to compete against the coronavirus disease 2019（COVID-19）pandemic. DDS mRNA therapeutics are generally produced by in vitro transcription utilizing RNA polymerase mediated elongation. However, its purity, stability, and protein synthesis ability, are difficult to be precisely controlled, which is pointed out as drawbacks that must be overcome. To overcome these issues, the introduction of chemically modified nucleic acids is focusing attention. However, it is difficult to flexible molecular design due to the requirement of RNA polymerase recognition ability of chemically modified nucleic acids under in vitro transcription reaction. In the future, the development of a new mRNA design concept based on a flexible molecular design by the progress of chemically modified mRNA therapeutics synthesis method. Under the situation, the authors are focusing on the translation mechanism of mRNA and proposing a new mRNA molecular design to accelerate the translation reaction cycle. In this paper, we introduce an update on therapeutic mRNA design. © 2022, Japan Society of Drug Delivery System. All rights reserved.","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49160231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"For developing next generation of mRNA medicines and vaccines","authors":"K. Itaka","doi":"10.2745/dds.37.191","DOIUrl":"https://doi.org/10.2745/dds.37.191","url":null,"abstract":"","PeriodicalId":11331,"journal":{"name":"Drug Delivery System","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45513611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}