Drug Target Insights最新文献_第8页

Preliminary In Vivo Evaluation of a Hybrid Armored Vascular Graft Combining Electrospinning and Additive Manufacturing Techniques 结合静电纺丝和增材制造技术的混合装甲血管移植物的初步体内评价

IF 2.7

Drug Target Insights Pub Date : 2016-01-01 DOI: 10.4137/DTI.S35202

C. Spadaccio, F. Nappi, F. De Marco, P. Sedati, Fraser W. H. Sutherland, M. Chello, M. Trombetta, A. Rainer

引用次数: 32

Basal Plasma Levels of Copeptin are Elevated in Inactive Inflammatory Bowel Disease after Bowel Resection. 非活动性炎性肠病患者肠切除术后基础血浆Copeptin水平升高

IF 2.7

Drug Target Insights Pub Date : 2015-07-13 eCollection Date: 2015-01-01 DOI: 10.4137/DTI.S26589

Bodil Ohlsson, Olle Melander

{"title":"Basal Plasma Levels of Copeptin are Elevated in Inactive Inflammatory Bowel Disease after Bowel Resection.","authors":"Bodil Ohlsson, Olle Melander","doi":"10.4137/DTI.S26589","DOIUrl":"https://doi.org/10.4137/DTI.S26589","url":null,"abstract":"Evidence of interactions between the enteric nervous system, neuropeptides, and the immune system is growing. The aim of this study was to examine basal plasma levels of a variety of peptide precursors in patients with inflammatory bowel disease (IBD). In two middle-aged cohorts, Malmö Preventive Medicine (n = 5,415) and Malmö Diet and Cost Study (n = 6,103), individuals with the diagnosis of IBD were identified. Medical records were scrutinized. Three controls were matched for each patient. Copeptin, midregional fragments of adrenomedullin, pro-atrial natriuretic peptide, and proenkephalin A, as well as N-terminal protachykinin A and proneurotensin were analyzed in the plasma. Sixty-two IBD patients were identified. The only difference between patients and controls was higher copeptin levels in the patients compared with controls (P = 0.006), with higher copeptin levels in resected than unresected patients (P = 0.020). There was no difference in any precursor levels between Crohn's disease and ulcerative colitis, between different distributions of disease lesions, or between different treatments. ","PeriodicalId":11326,"journal":{"name":"Drug Target Insights","volume":"9 ","pages":"21-7"},"PeriodicalIF":2.7,"publicationDate":"2015-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/DTI.S26589","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33897047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Medicinal Plants: A Potential Source of Compounds for Targeting Cell Division. 药用植物:靶向细胞分裂化合物的潜在来源。

IF 2.7

Drug Target Insights Pub Date : 2015-06-15 eCollection Date: 2015-01-01 DOI: 10.4137/DTI.S24946

Ihsan N Zulkipli, Sheba R David, Rajan Rajabalaya, Adi Idris

引用次数: 32

7-NI and ODQ Disturbs Memory in the Elevated Plus Maze, Morris Water Maze, and Radial Arm Maze Tests in Mice. 7-NI和ODQ对高架迷宫、Morris水迷宫和桡臂迷宫小鼠记忆的干扰

IF 2.7

Drug Target Insights Pub Date : 2015-03-04 eCollection Date: 2015-01-01 DOI: 10.4137/DTI.S23378

Oguz Mutlu, Furuzan Akar, Ipek Komsuoglu Celikyurt, Pelin Tanyeri, Guner Ulak, Faruk Erden

{"title":"7-NI and ODQ Disturbs Memory in the Elevated Plus Maze, Morris Water Maze, and Radial Arm Maze Tests in Mice.","authors":"Oguz Mutlu, Furuzan Akar, Ipek Komsuoglu Celikyurt, Pelin Tanyeri, Guner Ulak, Faruk Erden","doi":"10.4137/DTI.S23378","DOIUrl":"https://doi.org/10.4137/DTI.S23378","url":null,"abstract":"Nitric oxide (NO) is an atypical neurotransmitter that causes changes in cognition. Nitric oxide synthase (NOS) and guanylate cyclase (GC) inhibitors have been shown to exert some effects on cognition in previous studies; however, the findings have been controversial. This study was aimed at understanding the effects of an NOS inhibitor, 7-nitroindazole (7-NI), and a guanylate cyclase inhibitor, 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ), on spatial memory in modified elevated plus maze (mEPM), Morris water maze (MWM), and radial arm maze (RAM) tests. Male Balb-c mice were treated via intraperitoneal injections with 7-NI (15 mg/kg), ODQ (3, 10 mg/kg), L-arginine (100 mg/kg) + 7-NI (15 mg/kg), or physiological saline. ODQ (3 mg/kg) and 7-NI (15 mg/kg) significantly increased the second-day latency in the mEPM test. 7-NI (15 mg/kg) and ODQ (10 mg/kg) significantly increased the escape latency in second, third, and fourth sessions, decreased the time spent in the escape platform's quadrant, and increased the mean distance to the platform in the probe trial of the MWM test. ODQ (3, 10 mg/kg) and 7-NI (15 mg/kg) significantly increased the number of errors, whereas only 7-NI increased the latency in the RAM test. The administration of L-arginine (100 mg/kg) prior to 7-NI inverted the effects of 7-NI, which supports the role of NO on cognition. Our study shows that the NO/cGMP/GS pathway can regulate spatial memory in mice. ","PeriodicalId":11326,"journal":{"name":"Drug Target Insights","volume":"9 ","pages":"1-8"},"PeriodicalIF":2.7,"publicationDate":"2015-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/DTI.S23378","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33145207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Screening Analogs of β-OG Pocket Binder as Fusion Inhibitor of Dengue Virus 2 登革病毒2型融合抑制剂β-OG口袋结合物的筛选

IF 2.7

Drug Target Insights Pub Date : 2015-01-01 DOI: 10.4137/DTI.S31566

Usman SF Tambunan, H. Zahroh, A. A. Parikesit, Syarifuddin Idrus, D. Kerami

引用次数: 19

Management of Diabetes Associated with Nephrotic Syndrome: Therapeutic Potential of Dapagliflozin for Protracted Volume Retention 糖尿病肾病综合征的管理:达格列净治疗持续性容量潴留的潜力

IF 2.7

Drug Target Insights Pub Date : 2015-01-01 DOI: 10.4137/DTI.S31710

T. Imai, T. Akimoto, C. Ito, Takahiro Masuda, D. Nagata

引用次数: 12

Genome-wide Analysis of Mycoplasma hominis for the Identification of Putative Therapeutic Targets. 人支原体基因组分析鉴定推定治疗靶点。

IF 2.7

Drug Target Insights Pub Date : 2014-12-09 eCollection Date: 2014-01-01 DOI: 10.4137/DTI.S19728

Md Masud Parvege, Monzilur Rahman, Mohammad Shahnoor Hossain

引用次数: 8

The Expression of Serum Antibodies Against Gonadotropin-releasing Hormone (GnRH1), Progonadoliberin-2, Luteinizing Hormone (LH), and Related Receptors in Patients with Gastrointestinal Dysfunction or Diabetes Mellitus. 胃肠功能紊乱或糖尿病患者血清中促性腺激素释放激素 (GnRH1)、前列腺素-2、促黄体生成素 (LH) 及相关受体抗体的表达。

IF 2.7

Drug Target Insights Pub Date : 2014-11-10 eCollection Date: 2014-01-01 DOI: 10.4137/DTI.S19352

Bodil Roth, Kerstin Berntorp, Bodil Ohlsson

{"title":"The Expression of Serum Antibodies Against Gonadotropin-releasing Hormone (GnRH1), Progonadoliberin-2, Luteinizing Hormone (LH), and Related Receptors in Patients with Gastrointestinal Dysfunction or Diabetes Mellitus.","authors":"Bodil Roth, Kerstin Berntorp, Bodil Ohlsson","doi":"10.4137/DTI.S19352","DOIUrl":"10.4137/DTI.S19352","url":null,"abstract":"Gonadotropin-releasing hormone (GnRH) 1 and 2 and luteinizing hormone (LH) receptors have been described in the gastrointestinal tract. We have previously demonstrated antibodies in serum against GnRH1 in patients with gastrointestinal dysfunction and diabetes mellitus, and antibodies against GnRH receptor, LH, and LH receptor in patients with infertility. The aim of this study was to search for the expression of serum antibodies against GnRH1 with an improved enzyme-linked immune sorbent assay (ELISA), and antibodies against progonadoliberin-2, GnRH2, GnRH receptor, LH, and LH receptor with newly developed ELISAs, in patients with gastrointestinal dysfunction or diabetes mellitus. Healthy blood donors served as controls. Medical records were scrutinized. Our conclusion was that IgM antibodies against GnRH1, progonadoliberin-2, and/or GnRH receptors were more prevalent in patients with functional gastrointestinal disorders, gastrointestinal dysmotility, and/or diabetes mellitus, whereas IgG antibodies against these peptides, and LH- and LH receptor antibodies, were expressed in the same magnitude as in controls. ","PeriodicalId":11326,"journal":{"name":"Drug Target Insights","volume":"8 ","pages":"45-50"},"PeriodicalIF":2.7,"publicationDate":"2014-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4227618/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32861958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Febuxostat for hyperuricemia in patients with advanced chronic kidney disease. 非布司他治疗晚期慢性肾病患者的高尿酸血症。

IF 2.7

Drug Target Insights Pub Date : 2014-08-13 eCollection Date: 2014-01-01 DOI: 10.4137/DTI.S16524

Tetsu Akimoto, Yoshiyuki Morishita, Chiharu Ito, Osamu Iimura, Sadao Tsunematsu, Yuko Watanabe, Eiji Kusano, Daisuke Nagata

{"title":"Febuxostat for hyperuricemia in patients with advanced chronic kidney disease.","authors":"Tetsu Akimoto, Yoshiyuki Morishita, Chiharu Ito, Osamu Iimura, Sadao Tsunematsu, Yuko Watanabe, Eiji Kusano, Daisuke Nagata","doi":"10.4137/DTI.S16524","DOIUrl":"https://doi.org/10.4137/DTI.S16524","url":null,"abstract":"Febuxostat is a nonpurine xanthine oxidase (XO) inhibitor, which recently received marketing approval. However, information regarding the experience with this agent among advanced chronic kidney disease (CKD) patients is limited. In the current study, we investigated the effects of oral febuxostat in patients with advanced CKD with asymptomatic hyperuricemia. We demonstrated, for the first time, that not only the serum levels of uric acid (UA) but also those of 8-hydroxydeoxyguanosine, an oxidative stress marker, were significantly reduced after six months of febuxostat treatment, with no adverse events. These results encouraged us to pursue further investigations regarding the clinical impact of lowering the serum UA levels with febuxostat in advanced CKD patients in terms of concomitantly reducing oxidative stress via the blockade of XO. More detailed studies with a larger number of subjects and assessments of the effects of multiple factors affecting hyperuricemia, such as age, sex, and dietary habits, would shed light on the therapeutic challenges of treating asymptomatic hyperuricemia in patients with various stages of CKD. ","PeriodicalId":11326,"journal":{"name":"Drug Target Insights","volume":"8 ","pages":"39-43"},"PeriodicalIF":2.7,"publicationDate":"2014-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/DTI.S16524","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32659674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

Molecular and Kinetic Characterization of Babesia microti Gray Strain Lactate Dehydrogenase as a Potential Drug Target. 微巴贝斯虫灰色菌株乳酸脱氢酶作为潜在药物靶点的分子和动力学特性

IF 2.7

Drug Target Insights Pub Date : 2014-07-28 eCollection Date: 2014-01-01 DOI: 10.4137/DTI.S16504

Patrick Vudriko, Tatsunori Masatani, Shinuo Cao, Mohamad Alla Terkawi, Ketsarin Kamyingkird, Ahmed A Mousa, Paul F Adjou Moumouni, Yoshifumi Nishikawa, Xuenan Xuan

{"title":"Molecular and Kinetic Characterization of Babesia microti Gray Strain Lactate Dehydrogenase as a Potential Drug Target.","authors":"Patrick Vudriko, Tatsunori Masatani, Shinuo Cao, Mohamad Alla Terkawi, Ketsarin Kamyingkird, Ahmed A Mousa, Paul F Adjou Moumouni, Yoshifumi Nishikawa, Xuenan Xuan","doi":"10.4137/DTI.S16504","DOIUrl":"https://doi.org/10.4137/DTI.S16504","url":null,"abstract":"Babesia microti is an emerging zoonotic protozoan organism that causes \"malaria-like\" symptoms that can be fatal in immunocompromised people. Owing to lack of specific therapeutic regiment against the disease, we cloned and characterized B. microti lactate dehydrogenase (BmLDH) as a potential molecular drug receptor. The in vitro kinetic properties of BmLDH enzyme was evaluated using nicotinamide adenine dinucleotide (NAD(+)) as a co-factor and lactate as a substrate. Inhibitory assay was also done using gossypol as BmLDH inhibitor to determine the inhibitory concentration 50 (IC50). The result showed that the 0.99 kbp BmLDH gene codes for a barely soluble 36 kDa protein (332 amino acids) localized in both the cytoplasm and nucleus of the parasite. In vitro enzyme kinetic studies further revealed that BmLDH is an active enzyme with a high catalytic efficiency at optimal pH of 10.2. The K m values of NAD(+) and lactate were 8.7 ± 0.57 mM and 99.9 ± 22.33 mM, respectively. The IC50 value for gossypol was 0.345 μM, while at 2.5 μM, gossypol caused 100% inhibition of BmLDH catalytic activity. These findings, therefore, provide initial evidence that BmLDH could be a potential drug target, although further in vivo studies are needed to validate the practical application of lactate dehydrogenase inhibitors against B. microti infection. ","PeriodicalId":11326,"journal":{"name":"Drug Target Insights","volume":"8 ","pages":"31-8"},"PeriodicalIF":2.7,"publicationDate":"2014-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4137/DTI.S16504","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32587107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8