Drug Invention Today最新文献_第5页

Stability indicating spectrophotometric methods for determination of bumadizone in the presence of its alkaline degradation product 稳定性指示分光光度法测定碱降解产物中人类酮的含量

Drug Invention Today Pub Date : 2013-06-01 DOI: 10.1016/j.dit.2013.06.001

Hala E. Zaazaa , Nouruddin W. Ali , Maimana A. Magdy , Mohamed Abdelkawy

{"title":"Stability indicating spectrophotometric methods for determination of bumadizone in the presence of its alkaline degradation product","authors":"Hala E. Zaazaa , Nouruddin W. Ali , Maimana A. Magdy , Mohamed Abdelkawy","doi":"10.1016/j.dit.2013.06.001","DOIUrl":"10.1016/j.dit.2013.06.001","url":null,"abstract":"<div><h3>Objectives</h3><p>Simple, selective and precise stability indicating spectrophotometric methods were adopted and validated for the quantitative determination of Bumadizone calcium semihydrate (BUM) in presence of its alkaline degradation product (DEG I).</p></div><div><h3>Methods</h3><p><strong>Method A</strong> is based on the application of first derivative (<sup>1</sup>D) spectrophotometry, then measuring the amplitude of BUM at 245.4 nm. <strong>Method B</strong> depends on measuring the peak amplitude of the first derivative of the ratio spectra (<sup>1</sup>DD) at 242.6, 260 and 274 nm over a concentration range of 6–20 μg mL<sup>−1</sup>. <strong>Method C</strong> is isoabsorptive point spectrophotometry where total concentration of BUM and DEG I was calculated at their isoabsorptive point at 242.2 nm (Aiso) while DEG I concentration alone can be determined at <em>λ</em><sub>max</sub> 320 nm, and then BUM concentration can be determined by subtraction. In <strong>Method D</strong> ratio subtraction spectrophotometry is applied.</p></div><div><h3>Results</h3><p>The four methods were found to be specific for determination BUM in presence of different concentrations of DEG I and were successfully applied for the determination of BUM in Octomotol<sup>®</sup> tablets.</p></div><div><h3>Conclusion</h3><p>Statistical comparison between the results obtained by applying the proposed methods and that obtained by the manufacturer one for the determination of the drug was done, and it was found that there is no significant difference between them.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.06.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88438321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Design, synthesis and biological evaluation of some novel isoniazid cyclocondensed azetidinones 几种新型异烟肼环缩合氮杂二酮的设计、合成及生物学评价

Drug Invention Today Pub Date : 2013-06-01 DOI: 10.1016/j.dit.2013.05.007

Karthikeyan Elumalai , Mohammed Ashraf Ali , Manogaran Elumalai , Kalpana Eluri , Sivaneswari Srinivasan , Sujit Kumar Mohanti , Anil Thota

{"title":"Design, synthesis and biological evaluation of some novel isoniazid cyclocondensed azetidinones","authors":"Karthikeyan Elumalai , Mohammed Ashraf Ali , Manogaran Elumalai , Kalpana Eluri , Sivaneswari Srinivasan , Sujit Kumar Mohanti , Anil Thota","doi":"10.1016/j.dit.2013.05.007","DOIUrl":"10.1016/j.dit.2013.05.007","url":null,"abstract":"<div><h3>Background</h3><p>In the present study, a series of novel azetidinone derivatives synthesized because of its potent antimicrobial and antimycobacterial activity.</p></div><div><h3>Method</h3><p>Compounds (10a–10j) were synthesized by reacting Schiff's base of isoniazid reacted with, chloro acetyl chloride in presence of triethyl amine and 1, 4-dioxane as an efficient catalyst, analyzed for their structures. <em>in vitro</em> antimicrobial and antimycobacterial activity were carried out.</p></div><div><h3>Results</h3><p>Among the synthesized compounds, compound 10b and 10i was found to be the most potent against gram-positive bacteria <em>Bacillus subtilis,</em> gram-negative bacteria <em>Escherichia coli, Mycobacterium tuberculosis</em> CIP <em>and M. tuberculosis</em> H37Rv.</p></div><div><h3>Conclusion</h3><p>A series of novel azetidinone derivatives of biological interest were synthesized and analyzed, suggests that it an interesting compound compared to the current therapeutic agents and are considered to investigate further for the same.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.05.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81671105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Rasagiline hemitartrate: Synthesis, characterization and RP-HPLC validation for its estimation in bulk form 半酒石酸雷沙吉兰的合成、表征及原料药的RP-HPLC验证

Drug Invention Today Pub Date : 2013-06-01 DOI: 10.1016/j.dit.2013.05.002

Muvvala S. Sudhir , Ratnakaram Venkata Nadh

{"title":"Rasagiline hemitartrate: Synthesis, characterization and RP-HPLC validation for its estimation in bulk form","authors":"Muvvala S. Sudhir , Ratnakaram Venkata Nadh","doi":"10.1016/j.dit.2013.05.002","DOIUrl":"10.1016/j.dit.2013.05.002","url":null,"abstract":"<div><h3>Objectives</h3><p>To develop a reverse phase high performance liquid chromatographic method for validation and quantitative estimation of the synthesized drug rasagiline hemitartrate in bulk form.</p></div><div><h3>Methods</h3><p>Rasagiline hemitartrate was synthesized and characterized by spectral (Infrared, Proton Nuclear Magnetic Resonance and Mass) as well as elemental analysis. Chromatographic separation was conducted on Agilent TC-C18 (250 × 4.6 mm, 5 μm) column at ambient temperature using mixture of 20 mM potassium dihydrogen orthophosphate buffer (pH 7.0): methanol and acetonitrile in the ratio (30:30:40 v/v) as a mobile phase and at a flow rate of 1.0 mL/min, while UV detection was performed at 285 nm. In addition to LOD and LOQ, other analytical parameters viz., linearity, precision, accuracy, ruggedness and robustness were detected by following the ICH (International Conference on Harmonization) guidelines.</p></div><div><h3>Results</h3><p>The retention time for rasagiline hemitartrate was found to be 4.30 ± 0.05 min. The method was found to be linear in the range of 10–50 μg/mL. The limit of detection and quantization for rasagiline hemitartrate are found to be 0.651 and 1.972 μg/mL respectively. Analytical recovery was 100.47%. The percentage RSD for precision and accuracy of the method was found to be less than 2%. Correlation coefficient was found to be 0.9952.</p></div><div><h3>Conclusion</h3><p>In this study, simple, sensitive, accurate and reliable RP-HPLC method was developed and validated as per the ICH guidelines for the determination of the synthesized drug rasagiline hemitartrate in bulk form.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83761626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Synthesis of indazole substituted-1,3,4-thiadiazoles and their anticonvulsant activity 茚唑取代-1,3,4-噻二唑的合成及其抗惊厥活性

Drug Invention Today Pub Date : 2013-06-01 DOI: 10.1016/j.dit.2013.06.002

Kikkeri P. Harish , Kikkeri N. Mohana , Lingappa Mallesha

{"title":"Synthesis of indazole substituted-1,3,4-thiadiazoles and their anticonvulsant activity","authors":"Kikkeri P. Harish , Kikkeri N. Mohana , Lingappa Mallesha","doi":"10.1016/j.dit.2013.06.002","DOIUrl":"10.1016/j.dit.2013.06.002","url":null,"abstract":"<div><h3>Objectives</h3><p>To synthesize a new series of indazole substituted-1,3,4-thiadiazole derivatives <strong>7(a-l)</strong> and test the anticonvulsant activity against maximal electroshock (MES) seizure model in male Wistar rats.</p></div><div><h3>Methods</h3><p>New compounds were synthesized by the reaction of indazole substituted-1,3,4-thiadiazoles with various sulfonyl chlorides. The purity of the compounds was confirmed on the basis of their elemental analysis. Chemical structures of all the new compounds were established by <sup>1</sup>H NMR and mass spectral data. Anticonvulsant study was done by MES seizure model and rotorod method was employed to determine the neurotoxicity.</p></div><div><h3>Results</h3><p>All the compounds were synthesized in good yield. Out of twelve compounds, <strong>7b</strong> and <strong>7d</strong> are found to be most potent of this series. The same compounds showed no neurotoxicity at the maximum dose administered (100 mg/kg).</p></div><div><h3>Conclusions</h3><p>The results obtained justify the usage of these compounds from their promising anticonvulsant activity. Further studies are needed to fully characterize the toxicity and the mechanisms involved with the anticonvulsant activity and neurotoxicity of these compounds.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.06.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76826230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Interactions between carvedilol and sodium valproate along with neurobehavioural co-morbidities in various epilepsy models 卡维地洛和丙戊酸钠在各种癫痫模型中与神经行为共病的相互作用

Drug Invention Today Pub Date : 2013-06-01 DOI: 10.1016/j.dit.2013.05.003

Radha Goel , Amit Goel

{"title":"Interactions between carvedilol and sodium valproate along with neurobehavioural co-morbidities in various epilepsy models","authors":"Radha Goel , Amit Goel","doi":"10.1016/j.dit.2013.05.003","DOIUrl":"10.1016/j.dit.2013.05.003","url":null,"abstract":"<div><h3>Objective</h3><p>This study evaluated the effect of carvedilol (CRV) commonly used antihypertensive drugs on the antiepileptic drug sodium valproate (SVP) along with neurobehavioural co-morbidities in various models of epilepsy.</p></div><div><h3>Methods</h3><p>For this purpose, we used the Increasing Current Electroshock Seizure (ICES) test and Pentylenetetrazole (PTZ) test in mice. Additionally, adverse effects of combined treatment with the drugs in the Spontaneous Alternation Behavior (SAB) and rotarod were assessed. The levels of TBARS and the Reduced Glutathione (GSH) were determined in brains of mice for investigation of the oxidative stress. All drugs were administered orally.</p></div><div><h3>Results</h3><p>SVP (50 & 100 mg/kg) and CRV (1.25, 2.5 & 5.0 mg/kg) alone as well as in combination significantly enhanced the seizure threshold in the ICES test and PTZ test. CRV significantly increase the percentage alternation scores as compared to control group whereas combination of SVP with CRV did not affect the percentage alternation scores. In the present study, CRV and SVP alone as well as in combinations had no significant effect on motor parameters, at any of the given doses. CRV and SVP alone as well as in combination shown to inhibit the lipid peroxidation and increase in the level of GSH in brain tissue in a dose dependent manner which showed that it reduces the oxidative stress.</p></div><div><h3>Conclusion</h3><p>The current study suggests that CRV potentiate the anticonvulsant activity of VPA. Therefore, the observed interaction could be pharmacodynamics in nature.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77315084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Antibacterial study of silver doped zinc oxide nanoparticles against Staphylococcus aureus and Bacillus subtilis 银掺杂氧化锌纳米颗粒对金黄色葡萄球菌和枯草芽孢杆菌的抑菌研究

Drug Invention Today Pub Date : 2013-03-01 DOI: 10.1016/j.dit.2013.03.007

Neha Sharma , Jitender Kumar , Shaveta Thakur , Shruti Sharma , Vikas Shrivastava

{"title":"Antibacterial study of silver doped zinc oxide nanoparticles against Staphylococcus aureus and Bacillus subtilis","authors":"Neha Sharma , Jitender Kumar , Shaveta Thakur , Shruti Sharma , Vikas Shrivastava","doi":"10.1016/j.dit.2013.03.007","DOIUrl":"10.1016/j.dit.2013.03.007","url":null,"abstract":"<div><h3>Objectives</h3><p>The present study has been undertaken to synthesize silver doped zinc oxide nanoparticles, with pharmaceutical importance. The synthesized particles have been evaluated to study the effect of silver doping on grain size and further on antibacterial activities against the microorganisms <em>Bacillus subtilis</em> and <em>Staphylococcus aureus</em>.</p></div><div><h3>Methods</h3><p>Silver doped zinc oxide nanoparticles were prepared by the solution route spin-coating process, using zinc acetate (Zn(CH<sub>3</sub>COO)<sub>2</sub>.2H<sub>2</sub>O) and silver nitrate (AgNO<sub>3</sub>) as host and dopant precursors respectively. The antibacterial activity of the silver doped zinc oxide were studied against <em>S. auerus</em> and <em>B. subtilis</em> via using agar well diffusion method.</p></div><div><h3>Results & discussion</h3><p>: The structure of the powder samples was analyzed by X-ray diffraction (XRD). The effect of silver doping on grain size and further on antibacterial activity against the microorganisms <em>B. subtilis</em> and <em>S. auerus</em> is discussed.</p></div><div><h3>Conclusion</h3><p>It was clear from X-ray investigations that its structure is wurtzite type and that an increase in Ag-doping resulted in decrease in the grain size of the ZnO nanoparticles. Antimicrobial study against the microorganisms <em>B. subtilis</em> and <em>S. auerus</em> shows that in case of <em>S. auerus</em> the MIC varies with increase in Ag content but in case of <em>B. subtilis</em> the MIC remained constant for all concentration of Ag.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.03.007","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91540178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 97

In vivo study of clobetasol propionate loaded nanoemulsion for topical application in psoriasis and atopic dermatitis 负载丙酸氯倍他索纳米乳局部应用于银屑病和特应性皮炎的体内研究

Drug Invention Today Pub Date : 2013-03-01 DOI: 10.1016/j.dit.2013.02.001

Md. Sarfaraz Alam , Md. Sajid Ali , Nawazish Alam , Masoom Raza Siddiqui , Md. Shamim , M.M. Safhi

{"title":"In vivo study of clobetasol propionate loaded nanoemulsion for topical application in psoriasis and atopic dermatitis","authors":"Md. Sarfaraz Alam , Md. Sajid Ali , Nawazish Alam , Masoom Raza Siddiqui , Md. Shamim , M.M. Safhi","doi":"10.1016/j.dit.2013.02.001","DOIUrl":"10.1016/j.dit.2013.02.001","url":null,"abstract":"<div><h3>Objectives</h3><p>Optimized formulations were subjected to various <em>In vivo</em> studies like anti-inflammatory activity, Nickel induced dermatitis and irritation study. Clobetasol propionate (CP) has anti-inflammatory, immunomodulatory, and antiproliferative activity. The aim of the present work was to test the hypothesis that the addition CP in nanoemulsions would result in enhancement CP delivery and leading to better antipsoriatic activity.</p></div><div><h3>Materials and methods</h3><p>Nanoemulsions were prepared by aqueous phase titration method, using eucalyptus oil, Tween 20, ethanol, and distilled water as the oil phase, surfactant, co-surfactant and aqueous phase, respectively.</p></div><div><h3>Results and discussion</h3><p>We developed a topical O/W nanoemulsion in which drug is incorporated in disperse phase of oil and evaluated its efficacy against different types of <em>in vivo</em> studies. It was also found that the significantly increased their anti-inflammatory activity. It was reported that CP-loaded nanoemulsion significantly increased NTPDase (Nucleoside triphosphate diphosphohydrolases) activity in lymphocytes. This membrane protein is responsible for the hydrolysis of extracellular ATP (Adenosine triphosphate) which is responsible for cell proliferation, differentiation and inflammatory processes. <em>In vivo</em> irritation studies did not show any irritation in spite of having high amount of surfactant.</p></div><div><h3>Conclusion</h3><p>On the basis of above <em>in vivo</em> study we conclude that developed nanoemulsion is safe for human use because it has good anti-inflammatory action and did not show any irritation to the skin. All though nanoemulsion contain high amount of surfactant in comparison to cream.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.02.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90051230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Probiotics in aquaculture 水产养殖中的益生菌

Drug Invention Today Pub Date : 2013-03-01 DOI: 10.1016/j.dit.2013.03.003

Priyadarshini Pandiyan , Deivasigamani Balaraman , Rajasekar Thirunavukkarasu , Edward Gnana Jothi George , Kumaran Subaramaniyan , Sakthivel Manikkam , Balamurugan Sadayappan

{"title":"Probiotics in aquaculture","authors":"Priyadarshini Pandiyan , Deivasigamani Balaraman , Rajasekar Thirunavukkarasu , Edward Gnana Jothi George , Kumaran Subaramaniyan , Sakthivel Manikkam , Balamurugan Sadayappan","doi":"10.1016/j.dit.2013.03.003","DOIUrl":"10.1016/j.dit.2013.03.003","url":null,"abstract":"<div><p>Aquaculture is the world's fastest growing food production sector. However, fish culture is currently suffering from serious losses due to infectious diseases. The use of antimicrobial drugs, pesticides and disinfectant in aquaculture disease prevention and growth promotion has led to the evolution of resistant strains of bacteria. Thus, the research into the use of probiotics for aquaculture is increasing with the demand for environment – friendly sustainable aquaculture. The benefits of such supplements include improved feed value, enzymatic contribution to digestion, inhibition of pathogenic microorganisms, anti-mutagenic and anti-carcinogenic activity, and increased immune response. These probiotics are harmless bacteria that help the well being of the host animal and contribute, directly or indirectly to protect the host animal against harmful bacterial pathogens. The use of probiotics in aquaculture has just begun, due to the fact that gastrointestinal microbiota of aquatic organisms has been poorly characterized, and their effects are not studied extensively. This review summarizes and evaluates brief knowledge about the probiotic organism, the action of probiotic in fish culture and the safety evaluation of probiotics in aquaculture.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.03.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77817176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 134

In vitro antiviral and cytotoxic screening of methanolic extract of Cassia auriculata flowers in HeLa, Vero, CRFK and HEL cell lines 黑木香花甲醇提取物对HeLa、Vero、CRFK和HEL细胞系的体外抗病毒和细胞毒性筛选

Drug Invention Today Pub Date : 2013-03-01 DOI: 10.1016/j.dit.2013.03.001

Saravanakumar Arthanari , Jayachandran Vanitha , Venkateshwaran Krishnaswami , Ponnusamy Renukadevi , Karthikeyan Deivasigamani , Eric De Clercq

{"title":"In vitro antiviral and cytotoxic screening of methanolic extract of Cassia auriculata flowers in HeLa, Vero, CRFK and HEL cell lines","authors":"Saravanakumar Arthanari , Jayachandran Vanitha , Venkateshwaran Krishnaswami , Ponnusamy Renukadevi , Karthikeyan Deivasigamani , Eric De Clercq","doi":"10.1016/j.dit.2013.03.001","DOIUrl":"10.1016/j.dit.2013.03.001","url":null,"abstract":"<div><h3>Background</h3><p>To investigate the <em>in vitro</em> antiviral and cytotoxic activities of methanolic extract of <em>Cassia auriculata</em> flowers in HeLa, Vero, CRFK and HEL Cell lines.</p></div><div><h3>Methods</h3><p>Polarity based cold maceration method was adopted for the extraction of flowers and the antiviral activity was assessed against herpes simplex-1 and 2, vaccinia, vesicular stomatitis, cox sackie, respiratory syncytical, feline corona, feline herpes, para influenza, reo-1, sindbis and punta toro viruses in different cell lines, such as Hel, HeLa, Crandell Reus feline kidney and Vero cell cultures. In parallel the cytotoxicity was evaluated by MTT assay method.</p></div><div><h3>Results</h3><p>The methanolic extract possesses the strongest antiviral activity against herpes simplex-1(EC<sub>50</sub> = 50 μg/ml) and 2 (EC<sub>50</sub> = 45 μg/ml) viruses and moderate activity for remaining viruses (EC<sub>50</sub> = >100 μg/ml). The cytotoxicity results revealed that the extracts exhibited cytotoxicity above the range of 100 μg/ml.</p></div><div><h3>Conclusion</h3><p>The tested methanolic extract displayed an important source of anti-herpetic compound against the double stranded DNA enveloped Herpes simplex virus (HSV-1 and II), further studies are needed to identify the exact compound for their antimicrobial action.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84465754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Spectrophotometric determination and thermodynamic studies of the charge transfer complexes of bambuterol–HCl 班布特罗-盐酸电荷转移配合物的分光光度测定及热力学研究

Drug Invention Today Pub Date : 2013-03-01 DOI: 10.1016/j.dit.2013.03.004

Wafaa E. Hassan , Sherief M. Eid , Khadiga M. Kelani , Abdalla A. Shalaby

{"title":"Spectrophotometric determination and thermodynamic studies of the charge transfer complexes of bambuterol–HCl","authors":"Wafaa E. Hassan , Sherief M. Eid , Khadiga M. Kelani , Abdalla A. Shalaby","doi":"10.1016/j.dit.2013.03.004","DOIUrl":"10.1016/j.dit.2013.03.004","url":null,"abstract":"<div><h3>Objective</h3><p>The aim of the present work was to develop simple spectrophotometric methods for the simultaneous determination of bambuterol hydrochloride.</p></div><div><h3>Method</h3><p>Spectrophotometric method for determination of bambuterol hydrochloride after its conversion to bambuterol base by formation of charge transfer complexes as n-donor with π-acceptors, dichlorodicyanobenzoquinone (DDQ) and tetracyanobenzoquinodimethane (TCNQ) which were prepared in acetonitrile. They yield radical anions measured at 461 and 842 nm within concentration ranges of 36.7–183.7, 4.59–36.7 μg ml<sup>−1</sup> with a good correlation coefficients (<em>r</em><sup>2</sup> = 0.9999–0.9998) respectively. The nature of the formed complexes was studied via determination of the association constant and the molar absorptivity using Bensi–Hildebrand equation. The free energy change (ΔG) and the enthalpy of formation (ΔH) as well as the entropy (ΔS) were determined for the reaction product with TCNQ. The method was successfully applied for the analysis of bambuterol hydrochloride in its pharmaceutical preparation where no interference could be observed from the additives commonly present as proven by good mean recoveries of 99.74% and 100.014%. There was no significant difference observed when the method was statistically compared with the pharmacopeial official method used for determination.</p></div><div><h3>Conclusion</h3><p>The proposed charge transfer complexation methods are rapid and simple and from the economical point of view, the analytical reagents used are inexpensive, have excellent shelf life and are available in any analytical laboratory. The suggested methods could be successfully applied for quality control and routine analysis.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.03.004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80417628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19