Saravanakumar Arthanari , Jayachandran Vanitha , Venkateshwaran Krishnaswami , Ponnusamy Renukadevi , Karthikeyan Deivasigamani , Eric De Clercq
{"title":"黑木香花甲醇提取物对HeLa、Vero、CRFK和HEL细胞系的体外抗病毒和细胞毒性筛选","authors":"Saravanakumar Arthanari , Jayachandran Vanitha , Venkateshwaran Krishnaswami , Ponnusamy Renukadevi , Karthikeyan Deivasigamani , Eric De Clercq","doi":"10.1016/j.dit.2013.03.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>To investigate the <em>in vitro</em> antiviral and cytotoxic activities of methanolic extract of <em>Cassia auriculata</em> flowers in HeLa, Vero, CRFK and HEL Cell lines.</p></div><div><h3>Methods</h3><p>Polarity based cold maceration method was adopted for the extraction of flowers and the antiviral activity was assessed against herpes simplex-1 and 2, vaccinia, vesicular stomatitis, cox sackie, respiratory syncytical, feline corona, feline herpes, para influenza, reo-1, sindbis and punta toro viruses in different cell lines, such as Hel, HeLa, Crandell Reus feline kidney and Vero cell cultures. In parallel the cytotoxicity was evaluated by MTT assay method.</p></div><div><h3>Results</h3><p>The methanolic extract possesses the strongest antiviral activity against herpes simplex-1(EC<sub>50</sub> = 50 μg/ml) and 2 (EC<sub>50</sub> = 45 μg/ml) viruses and moderate activity for remaining viruses (EC<sub>50</sub> = >100 μg/ml). The cytotoxicity results revealed that the extracts exhibited cytotoxicity above the range of 100 μg/ml.</p></div><div><h3>Conclusion</h3><p>The tested methanolic extract displayed an important source of anti-herpetic compound against the double stranded DNA enveloped Herpes simplex virus (HSV-1 and II), further studies are needed to identify the exact compound for their antimicrobial action.</p></div>","PeriodicalId":11284,"journal":{"name":"Drug Invention Today","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.dit.2013.03.001","citationCount":"9","resultStr":"{\"title\":\"In vitro antiviral and cytotoxic screening of methanolic extract of Cassia auriculata flowers in HeLa, Vero, CRFK and HEL cell lines\",\"authors\":\"Saravanakumar Arthanari , Jayachandran Vanitha , Venkateshwaran Krishnaswami , Ponnusamy Renukadevi , Karthikeyan Deivasigamani , Eric De Clercq\",\"doi\":\"10.1016/j.dit.2013.03.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p>To investigate the <em>in vitro</em> antiviral and cytotoxic activities of methanolic extract of <em>Cassia auriculata</em> flowers in HeLa, Vero, CRFK and HEL Cell lines.</p></div><div><h3>Methods</h3><p>Polarity based cold maceration method was adopted for the extraction of flowers and the antiviral activity was assessed against herpes simplex-1 and 2, vaccinia, vesicular stomatitis, cox sackie, respiratory syncytical, feline corona, feline herpes, para influenza, reo-1, sindbis and punta toro viruses in different cell lines, such as Hel, HeLa, Crandell Reus feline kidney and Vero cell cultures. In parallel the cytotoxicity was evaluated by MTT assay method.</p></div><div><h3>Results</h3><p>The methanolic extract possesses the strongest antiviral activity against herpes simplex-1(EC<sub>50</sub> = 50 μg/ml) and 2 (EC<sub>50</sub> = 45 μg/ml) viruses and moderate activity for remaining viruses (EC<sub>50</sub> = >100 μg/ml). The cytotoxicity results revealed that the extracts exhibited cytotoxicity above the range of 100 μg/ml.</p></div><div><h3>Conclusion</h3><p>The tested methanolic extract displayed an important source of anti-herpetic compound against the double stranded DNA enveloped Herpes simplex virus (HSV-1 and II), further studies are needed to identify the exact compound for their antimicrobial action.</p></div>\",\"PeriodicalId\":11284,\"journal\":{\"name\":\"Drug Invention Today\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/j.dit.2013.03.001\",\"citationCount\":\"9\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Drug Invention Today\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0975761913000069\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Drug Invention Today","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0975761913000069","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
In vitro antiviral and cytotoxic screening of methanolic extract of Cassia auriculata flowers in HeLa, Vero, CRFK and HEL cell lines
Background
To investigate the in vitro antiviral and cytotoxic activities of methanolic extract of Cassia auriculata flowers in HeLa, Vero, CRFK and HEL Cell lines.
Methods
Polarity based cold maceration method was adopted for the extraction of flowers and the antiviral activity was assessed against herpes simplex-1 and 2, vaccinia, vesicular stomatitis, cox sackie, respiratory syncytical, feline corona, feline herpes, para influenza, reo-1, sindbis and punta toro viruses in different cell lines, such as Hel, HeLa, Crandell Reus feline kidney and Vero cell cultures. In parallel the cytotoxicity was evaluated by MTT assay method.
Results
The methanolic extract possesses the strongest antiviral activity against herpes simplex-1(EC50 = 50 μg/ml) and 2 (EC50 = 45 μg/ml) viruses and moderate activity for remaining viruses (EC50 = >100 μg/ml). The cytotoxicity results revealed that the extracts exhibited cytotoxicity above the range of 100 μg/ml.
Conclusion
The tested methanolic extract displayed an important source of anti-herpetic compound against the double stranded DNA enveloped Herpes simplex virus (HSV-1 and II), further studies are needed to identify the exact compound for their antimicrobial action.
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