Diseases of aquatic organisms最新文献_第9页

Marteilia pararefringens infections are more frequent than revealed by the Norwegian surveillance programme, highlighting the need for its improvement. 副猪嗜血杆菌（Marteilia pararefringens）感染的频率比挪威监测计划显示的频率更高，这凸显了改进该计划的必要性。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-05-30 DOI: 10.3354/dao03785

Mats Bøgwald, Stein Mortensen

{"title":"Marteilia pararefringens infections are more frequent than revealed by the Norwegian surveillance programme, highlighting the need for its improvement.","authors":"Mats Bøgwald, Stein Mortensen","doi":"10.3354/dao03785","DOIUrl":"https://doi.org/10.3354/dao03785","url":null,"abstract":"Norway had historically been considered free of marteiliosis in bivalves since the disease surveillance programme began in 1995. However, in 2016, Marteilia pararefringens, a protistan parasite of mussels Mytilus spp., was described in a heliothermic lagoon-a poll-previously used to produce flat oyster spat. To study whether the parasite was introduced, and possibly spread, via the historical flat oyster networks on the south and west coast, we sampled aquaculture polls that were part of different networks of farmers and wild, natural polls with no aquaculture activity. Additionally, we sampled mussel banks influenced by polls and sheltered bays that could have a similar environment to that of polls. We identified 7 sites with M. pararefringens-infected mussel populations: 5 were polls used in flat oyster production and 2 were in fjord areas with no known connection to any bivalve aquaculture. Prevalence ranged between 2 and 88%. At one site, Trysfjorden, we found M. pararefringens in atypical organs, including the gills, mantle, and intestine. Marteilia-like cells were also observed in the epithelium, lumen, and surrounding connective tissue of metanephridia and in the sinus of the anterior retractor muscle. Our results demonstrate that the parasite is more widespread than previously thought and is neither isolated to polls nor connected directly to aquaculture activity. Lastly, our findings highlight the need for an improved sampling strategy in surveillance programmes to detect marteiliosis in mussels.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"158 ","pages":"157-172"},"PeriodicalIF":1.4,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Widespread occurrence of the amphibian chytrid panzootic lineage in Uruguay is constrained by climate. 两栖动物糜烂泛祖系在乌拉圭的广泛出现受到气候的制约。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-05-30 DOI: 10.3354/dao03783

Claudio Borteiro, Gabriel Laufer, Noelia Gobel, Mailén Arleo, Francisco Kolenc, Sofía Cortizas, Diego A Barrasso, Rafael O de Sá, Alvaro Soutullo, Martin Ubilla, Claudio Martínez-Debat

{"title":"Widespread occurrence of the amphibian chytrid panzootic lineage in Uruguay is constrained by climate.","authors":"Claudio Borteiro, Gabriel Laufer, Noelia Gobel, Mailén Arleo, Francisco Kolenc, Sofía Cortizas, Diego A Barrasso, Rafael O de Sá, Alvaro Soutullo, Martin Ubilla, Claudio Martínez-Debat","doi":"10.3354/dao03783","DOIUrl":"https://doi.org/10.3354/dao03783","url":null,"abstract":"The amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) causes chytridiomycosis, a disease among the main causes of amphibian declines worldwide. However, Bd studies on Neotropical amphibians from temperate areas are scarce. We present a comprehensive survey of Bd in Uruguay, in temperate central eastern South America, carried out between 2006 and 2014. Skin swabs of 535 specimens of 21 native and exotic frogs were tested by PCR. We used individual-level data to examine the relationship between infection, climatic variables, and their effects on body condition and the number of prey items found in stomach contents. Infection was widespread in free-ranging anurans with an overall prevalence of 41.9%, detected in 15 native species, wild American bullfrogs Aquarana catesbeiana, and captive specimens of Ceratophrys ornata and Xenopus laevis. Three haplotypes of the Bd ITS region were identified in native amphibians, all belonging to the global panzootic lineage (BdGPL), of which only one was present in exotic hosts. Despite high infection frequencies in different anurans, we found no evidence of morbidity or mortality attributable to chytridiomycosis, and we observed no discernible impact on body condition or consumed prey. Climatic conditions at the time of our surveys suggested that the chance of infection is associated with monthly mean temperature, mean humidity, and total precipitation. Temperatures below 21°C combined with moderate humidity and pronounced rainfall may increase the likelihood of infection. Multiple haplotypes of BdGPL combined with high frequencies of infection suggest an enzootic pattern in native species, underscoring the need for continued monitoring.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"158 ","pages":"123-132"},"PeriodicalIF":1.4,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glove decontamination procedures to prevent pathogen and DNA cross-contamination among frogs. 防止青蛙病原体和 DNA 交叉感染的手套净化程序。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-05-30 DOI: 10.3354/dao03793

James E Noelker, Vitoria Abreu Ruozzi, Hunter M Craig, Jason P Sckrabulis, Thomas R Raffel

{"title":"Glove decontamination procedures to prevent pathogen and DNA cross-contamination among frogs.","authors":"James E Noelker, Vitoria Abreu Ruozzi, Hunter M Craig, Jason P Sckrabulis, Thomas R Raffel","doi":"10.3354/dao03793","DOIUrl":"https://doi.org/10.3354/dao03793","url":null,"abstract":"Working with aquatic organisms often requires handling multiple individuals in a single session, potentially resulting in cross-contamination by live pathogens or DNA. Most researchers address this problem by disposing of gloves between animals. However, this generates excessive waste and may be impractical for processing very slippery animals that might be easier to handle with cotton gloves. We tested methods to decontaminate cotton or nitrile gloves after contamination with cultured Batrachochytrium dendrobatidis (Bd) or after handling heavily Bd-infected Xenopus laevis with layered cotton and nitrile gloves. Bleach eliminated detectable Bd DNA from culture-contaminated nitrile gloves, but gloves retained detectable Bd DNA following ethanol disinfection. After handling a Bd-infected frog, Bd DNA contamination was greatly reduced by removal of the outer cotton glove, after which either bleach decontamination or ethanol decontamination followed by drying hands with a paper towel lowered Bd DNA below the detection threshold of our assay. These results provide new options to prevent pathogen or DNA cross-contamination, especially when handling slippery aquatic organisms. However, tradeoffs should be considered when selecting an animal handling procedure, such as the potential for cotton gloves to abrade amphibian skin or disrupt skin mucus. Disposing of gloves between animals should remain the gold standard for maintaining biosecurity in sensitive situations.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"158 ","pages":"173-178"},"PeriodicalIF":1.4,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141174880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence of Stenurus globicephalae (Nematoda: Pseudaliidae) in the blowhole of Globicephala macrorhynchus (Cetacea: Delphinidae) in Tasmania, Australia. 澳大利亚塔斯马尼亚 Globicephala macrorhynchus（鲸目目：海豚科）喷孔中 Stenurus globicephalae（线虫纲：伪蝶形目）的出现。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-05-08 DOI: 10.3354/dao03791

Shokoofeh Shamsi, František Moravec, Diane P Barton

引用次数: 0

Bacterial and viral co-infections in aquaculture under climate warming: co-evolutionary implications, diagnosis, and treatment 气候变暖条件下水产养殖中的细菌和病毒共同感染：共同进化的影响、诊断和治疗

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-04-11 DOI: 10.3354/dao03778

Sarahí Vega-Heredia, Ivone Giffard-Mena, Miriam Reverter

{"title":"Bacterial and viral co-infections in aquaculture under climate warming: co-evolutionary implications, diagnosis, and treatment","authors":"Sarahí Vega-Heredia, Ivone Giffard-Mena, Miriam Reverter","doi":"10.3354/dao03778","DOIUrl":"https://doi.org/10.3354/dao03778","url":null,"abstract":"ABSTRACT: Climate change and the associated environmental temperature fluctuations are contributing to increases in the frequency and severity of disease outbreaks in both wild and farmed aquatic species. This has a significant impact on biodiversity and also puts global food production systems, such as aquaculture, at risk. Most infections are the result of complex interactions between multiple pathogens, and understanding these interactions and their co-evolutionary mechanisms is crucial for developing effective diagnosis and control strategies. In this review, we discuss current knowledge on bacteria-bacteria, virus-virus, and bacterial and viral co-infections in aquaculture as well as their co-evolution in the context of global warming. We also propose a framework and different novel methods (e.g. advanced molecular tools such as digital PCR and next-generation sequencing) to (1) precisely identify overlooked co-infections, (2) gain an understanding of the co-infection dynamics and mechanisms by knowing species interactions, and (3) facilitate the development multi-pathogen preventive measures such as polyvalent vaccines. As aquaculture disease outbreaks are forecasted to increase both due to the intensification of practices to meet the protein demand of the increasing global population and as a result of global warming, understanding and treating co-infections in aquatic species has important implications for global food security and the economy.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"52 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2024-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140591096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of ectoparasite Lepeophtheirus salmonis counts on farmed Atlantic salmon and wild sea trout in Scotland. 苏格兰养殖大西洋鲑鱼和野生海鳟体外寄生虫 Lepeophtheirus salmonis 数量的关联。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03774

Stephen C Ives, Alexander G Murray, John D Armstrong

{"title":"Association of ectoparasite Lepeophtheirus salmonis counts on farmed Atlantic salmon and wild sea trout in Scotland.","authors":"Stephen C Ives, Alexander G Murray, John D Armstrong","doi":"10.3354/dao03774","DOIUrl":"10.3354/dao03774","url":null,"abstract":"Parasitic sea lice (Copepoda: Caligidae) colonising marine salmonid (Salmoniformes: Salmonidae) aquaculture production facilities have been implicated as a possible pressure on wild salmon and sea trout populations. This investigation uses monitoring data from the mainland west coast and Western Isles of Scotland to estimate the association of the abundance of adult female Lepeophtheirus salmonis (Krøyer) colonising farmed Atlantic salmon Salmo salar L. with the occurrence of juvenile and mobile L. salmonis on wild sea trout, anadromous S. trutta L. The associations were evaluated using generalised linear mixed models incorporating farmed adult female salmon louse abundances which are temporally lagged relative to dependent wild trout values. The pattern of lags, which is consistent with time for L. salmonis development between egg and infective stage, was evaluated using model deviances. A significant positive association is identified between adult female L. salmonis abundance on farms and juvenile L. salmonis on wild trout. This association is consistent with a causal relationship in which increases in the number of L. salmonis copepodids originating from lice colonising farmed Atlantic salmon cause an increase of L. salmonis abundance on wild sea trout.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"95-106"},"PeriodicalIF":1.4,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of in vitro treatments against the causative agent of Diadema antillarum scuticociliatosis (DaSc). 评估针对恙虫病（Diadema antillarum scuticociliatosis，DaSc）病原体的体外疗法。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03776

James S Evans, Julie J Voelschow, Isabella T Ritchie, Mya Breitbart, Ian Hewson, Christina A Kellogg

{"title":"Evaluation of in vitro treatments against the causative agent of Diadema antillarum scuticociliatosis (DaSc).","authors":"James S Evans, Julie J Voelschow, Isabella T Ritchie, Mya Breitbart, Ian Hewson, Christina A Kellogg","doi":"10.3354/dao03776","DOIUrl":"10.3354/dao03776","url":null,"abstract":"In the 1980s, a mass die-off of the long-spined sea urchin Diadema antillarum occurred on Florida and Caribbean coral reefs. D. antillarum populations largely did not recover, and in 2022, remaining populations experienced another mass mortality event. A ciliate most similar to Philaster apodigitiformis was identified as the causative agent of the 2022 event, which was named D. antillarum scuticociliatosis (DaSc). Here, we investigated possible treatments for this pathogen. We tested the efficacy of 10 compounds at final concentrations of 100, 50, 25, 12.5, 6.25, and 3.13 µM, or a 10-fold serial dilution series, against ciliates cultured from an infected D. antillarum specimen. Of the tested compounds, 8 induced 100% ciliate mortality at some dose after 24 h. The most effective (defined as those requiring the lowest dose to induce 100% ciliate mortality) were quinacrine and tomatine (both effective at 12.5 µM), followed by furaltadone and plumbagin (25 µM), bithionol sulfoxide and 2'4' dihydroxychalcone (50 µM), and oxyclozanide and carnidazole (100 µM). Toltrazuril and a commercially available anticiliate product containing naphthoquinones were not effective at any dose tested. Shortened (15 min) time trials were performed using ciliate cultures reared in natural seawater to better reflect natural environmental conditions, and revealed that 2 of the compounds (quinacrine and tomatine) induced 100% ciliate mortality at 100 µM, with tomatine also effective at 50 µM. This study identified several treatments effective against the causative agent of DaSc in vitro, but their toxicity and utility in vivo remain unknown.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"107-112"},"PeriodicalIF":1.4,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Habitat shift of adult Caligus undulatus (Copepoda: Siphonostomatoida: Caligidae) from host fish to plankton in response to host behavior. 桡足类：虹吸目：桡足科）成体根据宿主行为从宿主鱼类转移到浮游生物的栖息地。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03773

Yusuke Kondo, Susumu Ohtsuka, Masaki Nawata, Yusuke Nishida, Sota Komeda, Sadaharu Iwasaki, Panakkool Thamban Aneesh, Balu Alagar Venmathi Maran

{"title":"Habitat shift of adult Caligus undulatus (Copepoda: Siphonostomatoida: Caligidae) from host fish to plankton in response to host behavior.","authors":"Yusuke Kondo, Susumu Ohtsuka, Masaki Nawata, Yusuke Nishida, Sota Komeda, Sadaharu Iwasaki, Panakkool Thamban Aneesh, Balu Alagar Venmathi Maran","doi":"10.3354/dao03773","DOIUrl":"10.3354/dao03773","url":null,"abstract":"Before 2019, adults of the sea louse Caligus undulatus were reported exclusively in plankton from ocean samples worldwide and were not known to parasitize fish hosts. In 2019, the first instance of this caligid parasitizing a fish host, Japanese sardinella Sardinella zunasi, was reported in the Seto Inland Sea, Japan. The presently reported study aimed to investigate the biology and ecology of adult C. undulatus in plankton communities in the Seto Inland Sea and surrounding waters from March 2020 to November 2021. The occurrence of sea lice in plankton communities was restricted to the period of August-January, mainly between October and December with maximum plankton abundance (10.5 ind. per 1000 m3) recorded on 30 November 2020. All post-naupliar stages of C. undulatus were found on the host fish, and they represented a typical life cycle pattern known for Caligus species. The sex ratios in both planktonic and parasitic adults were not significantly different. The frequency of occurrence of planktonic and parasitic adult females with egg strings was 68 and 46%, respectively. The number of eggs per string was significantly higher in parasitic adult females (mean ± SD: 16.9 ± 8.6) than in planktonic females (10.4 ± 10.8). These data suggest that adult females were detached from their hosts and continued to produce eggs without feeding. Seasonal migration of S. zunasi to brackish water for spawning may result in the detachment of mature caligids from the host and may be effective in protecting the offspring, which are less tolerant of less brackish water.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"81-94"},"PeriodicalIF":1.4,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140119092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

First molecular confirmation of cyprinid herpesvirus 1 (CyHV1) in diseased carp in Serbia. 首次从分子角度证实塞尔维亚患病鲤鱼体内存在鲤科疱疹病毒 1 (CyHV1)。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03777

Vladimir Radosavljevic, Dimitrije Glisic, Jelena Maksimovic-Zoric, Ljubisa Veljovic, Bozidar Savic

引用次数: 0

Susceptibility of shellfish aquaculture species in the Chesapeake Bay and Maryland coastal bays to ostreid herpesvirus 1 microvariants. 切萨皮克湾和马里兰沿海海湾的贝类水产养殖物种对奥斯特里德疱疹病毒 1 微变种的易感性。

IF 1.4 4区农林科学

Diseases of aquatic organisms Pub Date : 2024-03-14 DOI: 10.3354/dao03775

M L Kachmar, K S Reece, M V Agnew, H J Schreier, C A Burge

{"title":"Susceptibility of shellfish aquaculture species in the Chesapeake Bay and Maryland coastal bays to ostreid herpesvirus 1 microvariants.","authors":"M L Kachmar, K S Reece, M V Agnew, H J Schreier, C A Burge","doi":"10.3354/dao03775","DOIUrl":"10.3354/dao03775","url":null,"abstract":"Ostreid herpesvirus 1 (OsHV-1) and its microvariants (µVars) cause economically devastating mass mortalities of oysters and pose a threat to the shellfish aquaculture industry globally. OsHV-1 outbreaks can cause up to 100% mortality in the Pacific oyster Crassostrea gigas. However, OsHV-1 and its variants have a broad host range and can infect at least 7 bivalve species, including bay scallops Argopecten irradians and eastern oysters C. virginica. Determining the susceptibility of economically and ecologically important bivalve species to OsHV-1 is critical for improving biosecurity and disease management to protect the aquaculture industry. Surveys of eastern oysters were conducted in June to August 2021 in the Maryland portion of the Chesapeake Bay to determine the prevalence and viral load of OsHV-1 at 5 aquaculture farms. Using quantitative PCR, OsHV-1 was not detected at any sites. Experiments examined the susceptibility of single stocks of eastern oysters and hard clams Mercenaria mercenaria to the virus and their ability to horizontally transmit it using OsHV-1 µVar SD (San Diego, California) and OsHV-1 µVar FRA (Marennes-Olreon, France). Results showed that OsHV-1 µVars did not cause mortality or symptomatic infection in the single stocks of eastern oysters and hard clams used in these experiments using natural infection pathways. However, the eastern oyster stock, when injected with OsHV-1, did transmit the virus to naïve Pacific oysters. Further experimentation using additional stocks and lines and establishment of surveillance programs along the east and Gulf coasts of the USA are necessary to prepare for the potential spread and impact of OsHV-1 related disease.","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"157 ","pages":"113-127"},"PeriodicalIF":1.4,"publicationDate":"2024-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140305203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0