Disease Markers最新文献

{"title":"Aldolase A Promotes Colorectal Cancer Progression through Targeting COPS6 and Regulating MAPK Signaling Pathway.","authors":"Ya Lu,&nbsp;Yuan Zhang,&nbsp;Xinyue Wang,&nbsp;Hui Zhang,&nbsp;Yue Zhu,&nbsp;Junying Zhang,&nbsp;Huanhuan Sha,&nbsp;Renrui Zou,&nbsp;Yujie Gan,&nbsp;Ying Sui,&nbsp;Juan Wang,&nbsp;Tongde Du,&nbsp;Jianzhong Wu,&nbsp;Jifeng Feng","doi":"10.1155/2023/1702125","DOIUrl":"https://doi.org/10.1155/2023/1702125","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a serious threat to human health, and its underlying mechanisms remain to be further explored. Aldolase A (ALDOA) has received increasing attention for its reported association with multiple cancers, but the role and mechanisms of ALDOA in CRC are still unclear. In the current study, high expression levels and enzymatic activity of ALDOA were detected in CRC tissues and cell lines, indicating the clinical significance of ALDOA in human CRC. In addition, silencing ALDOA significantly impaired the proliferation and metastasis of CRC cells <i>in vitro</i> and <i>in vivo</i>. Mechanistically, immunoprecipitation assays and mass spectrometry analysis identified the binding protein COPS6 of ALDOA. Furthermore, the promoting effects of upregulated ALDOA on CRC cell proliferation and metastasis were inhibited by COPS6 depletion, demonstrating COPS6 was required for ALDOA in mediating CRC progress. Moreover, the epithelial-mesenchymal transition (EMT) program and MAPK signaling pathway were found to be activated by ALDOA overexpression as well. In summary, our findings suggested that ALDOA facilitated the proliferation and metastasis of CRC by binding and regulating COPS6, inducing EMT, and activating the mitogen-activated protein kinase (MAPK) signaling pathway. The present study provided evidence for ALDOA as a promising potential biomarker for CRC.</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"1702125"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10344634/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9826205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of TIM-3 Expression and Its Correlation with TNF-<i>α</i> and IFN-<i>γ</i> in Patients with Surgically Resected Lung Adenocarcinoma.","authors":"Jian Tan,&nbsp;Piao Shen,&nbsp;Xingping Yang,&nbsp;Weijie Cai,&nbsp;Hongying Liao","doi":"10.1155/2023/2352945","DOIUrl":"https://doi.org/10.1155/2023/2352945","url":null,"abstract":"<p><strong>Objective: </strong>T cell immunoglobulin and mucin-containing protein-3 (TIM-3) is an important immune checkpoint, but its role in lung cancer is still not clear. In this study, we investigated TIM-3 protein expression and its correlation with TNF-<i>α</i> and IFN-<i>γ</i> by examining the tissues of patients with lung adenocarcinoma.</p><p><strong>Methods: </strong>We detected the mRNA quantity of TIM-3, TNF-<i>α</i>, and IFN-<i>γ</i> in 40 surgically resected specimens from patients with lung adenocarcinoma by real-time quantitative polymerase chain reaction (qRT-PCR). The protein expression of TIM-3, TNF-<i>α</i>, and IFN-<i>γ</i> was assessed in normal tissues, paracarcinoma tissues, and tumor tissues by western blotting, respectively. The relevance between the expression and clinicopathological information of the patients was analyzed.</p><p><strong>Results: </strong>The results showed that the expression level of TIM-3 was higher in tumor tissues than normal tissues and paracancerous tissues (<i>P</i> < 0.05). On the contrary, the expression of TNF-<i>α</i> and IFN-<i>γ</i> in tumor tissues was lower than normal tissues and paracarcinoma tissues (<i>P</i> < 0.05). However, the expression levels of IFN-<i>γ</i> mRNA were not observed to be significantly different between cancerous tissues and adjacent tissues. While TIM-3 protein expression in cancer tissues of patients with lymph node metastasis was higher than in patients without metastasis, the expression of TNF-<i>α</i> and IFN-<i>γ</i> was lower (<i>P</i> < 0.05). Importantly, the expression of TIM-3 was negatively correlated with the expression of TNF-<i>α</i> and IFN-<i>γ</i>, and the expression of TNF-<i>α</i> was found to be positively correlated with IFN-<i>γ</i> in the patient.</p><p><strong>Conclusion: </strong>The high expression of TIM-3, the low expression of TNF-<i>α</i> and IFN-<i>γ</i>, and the synergistic effect of TNF-<i>α</i> and IFN-<i>γ</i> in patients with lung adenocarcinoma were closely related to poor clinicopathological characteristics. Overexpression of TIM-3 may play an important role in the relationship between TNF-<i>α</i> and IFN-<i>γ</i> secretion and poor clinicopathological characteristics.</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"2352945"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9974258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9098209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: Functional Analysis of Serum Long Noncoding RNAs in Patients with Atrial Fibrillation.","authors":"Disease Markers","doi":"10.1155/2023/9893065","DOIUrl":"https://doi.org/10.1155/2023/9893065","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/2799123.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9893065"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: CKS2 and S100A12: Two Novel Diagnostic Biomarkers for Rheumatoid Arthritis.","authors":"Disease Markers","doi":"10.1155/2023/9871012","DOIUrl":"https://doi.org/10.1155/2023/9871012","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/2431976.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9871012"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371498/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: Overexpression of CAPG Is Associated with Poor Prognosis and Immunosuppressive Cell Infiltration in Ovarian Cancer.","authors":"Disease Markers","doi":"10.1155/2023/9898020","DOIUrl":"https://doi.org/10.1155/2023/9898020","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/9719671.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9898020"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371536/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10263776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: Activated Hepatic Stellate Cells Promote the M1 to M2 Macrophage Transformation and Liver Fibrosis by Elevating the Histone Acetylation Level.","authors":"Disease Markers","doi":"10.1155/2023/9754281","DOIUrl":"https://doi.org/10.1155/2023/9754281","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/9883831.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9754281"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307284/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9736997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: Antimicrobial Step-Down Therapy versus Conventional Antimicrobial Therapy in the Treatment of Patients with Sepsis.","authors":"Disease Markers","doi":"10.1155/2023/9830503","DOIUrl":"https://doi.org/10.1155/2023/9830503","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/3117805.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9830503"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9737022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"lncRNA SSTR5-AS1 Predicts Poor Prognosis and Contributes to the Progression of Esophageal Cancer.","authors":"Yuhao Hu,&nbsp;Ning Mao,&nbsp;Wei Zheng,&nbsp;Bin Hong,&nbsp;Xiong Deng","doi":"10.1155/2023/5025868","DOIUrl":"https://doi.org/10.1155/2023/5025868","url":null,"abstract":"<p><p>Esophageal cancer (ESCA), as a common cancer worldwide, is a main cause of cancer-related mortality. Long noncoding RNAs (lncRNAs) have been shown in an increasing number of studies to be capable of playing an important regulatory function in human malignancies. Our study is aimed at delving into the prognostic value and potential function of lncRNA SSTR5-AS1 (SSTR5-AS1) in ESCA. The gene expression data of 182 ESCA samples from TCGA and 653 nontumor specimens from GTEx. The expressions of SSTR5-AS1 were analyzed. We investigated whether there was a correlation between the expression of SSTR5-AS1 and the clinical aspects of ESCA. In order to compare survival curves, the Kaplan-Meier method together with the log-rank test was utilized. The univariate and multivariate Cox regression models were used to analyze the data in order to determine the SSTR5-AS1 expression's significance as a prognostic factor in ESCA patients. In order to investigate the level of SSTR5-AS1 expression in ESCA cells, RT-PCR was utilized. CCK-8 trials served as a model for the loss-of-function tests. In this study, we found that the expressions of SSTR5-AS1 were increased in ESCA specimens compared with nontumor specimens. According to the ROC assays, high SSTR5-AS1 expression had an AUC value of 0.7812 (95% CI: 0.7406 to 0.8217) for ESCA. Patients who had a high level of SSTR5-AS1 expression had a lower overall survival rate than those who had a low level of SSTR5-AS1 expression. In addition, multivariate analysis suggested that SSTR5-AS1 was an independent predictor of overall survival for ESCA patients. Moreover, RT-PCR experiments indicated that SSTR5-AS1 expression was distinctly increased in three ESCA cells compared with HET1A cells. CCK-8 experiments indicated that silence of SSTR5-AS1 distinctly inhibited the proliferation of ESCA cells. Overall, ESCA patients with elevated SSTR5-AS1 had a worse chance of survival, suggesting it could be used as a prognostic and diagnostic biomarker for ESCA.</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"5025868"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9886483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10647691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: Efficacy of Laparoscopic Totally Extraperitoneal Repair for Inguinal Hernia.","authors":"Disease Markers","doi":"10.1155/2023/9891012","DOIUrl":"https://doi.org/10.1155/2023/9891012","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/2970257.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9891012"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10659884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-9a-5p Protects Ischemic Stroke by Regulating Oxidative Stress and Mitochondrial Autophagy.","authors":"Chunli Ma,&nbsp;Qing Gao,&nbsp;Li Zhang,&nbsp;Geng Wu,&nbsp;Chao Li,&nbsp;Jun Chen,&nbsp;Yuxuan Fu,&nbsp;Lei Yang","doi":"10.1155/2023/5146305","DOIUrl":"https://doi.org/10.1155/2023/5146305","url":null,"abstract":"<p><strong>Purpose: </strong>Present research is aimed at exploring the effect of miR-9a-5p on mitochondrial autophagy and alleviating cellular oxidative stress injury in ischemic stroke.</p><p><strong>Methods: </strong>SH-SY5Y cells were cultured with oxygen-glucose deprivation/reoxygenation (OGD/R) to simulate ischemia/reperfusion. The cells were treated in an anaerobic incubator (95% N₂, 5% CO₂) for 2 h and then reoxygenated in the normoxic condition for 24 h with 2 ml of normal medium. Cells were transfected with miR-9a-5p mimic/inhibitor or negative control. The RT-qPCR assay was utilized to measure the mRNA expression. Western blot was utilized to evaluate the protein expression. The CCK-8 assay was conducted to detect cell viability. Flow cytometry was applied to examine apoptosis and the cell cycle. The ELISA assay was applied to measure the contents of SOD and MDA in mitochondria. Autophagosomes were observed via electron microscopy.</p><p><strong>Results: </strong>By comparison with the control group, the miR-9a-5p expression in the OGD/R group obviously declined. Mitochondrial crista breaks, vacuole-like changes, and increased autophagosome formation were observed in the OGD/R group. OGD/R injury enhanced oxidative stress damage and mitophagy. When transfected with the miR-9a-5p mimic, mitophagosome production of SH-SY5Y cells decreased and oxidative stress injury was inhibited. However, the miR-9a-5p inhibitor obviously increased mitophagosome production and enhanced oxidative stress injury.</p><p><strong>Conclusion: </strong>miR-9a-5p protects against ischemic stroke by inhibiting OGD/R-induced mitochondrial autophagy and alleviating cellular oxidative stress injury.</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"5146305"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9957637/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10793671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}