Disease Markers最新文献

{"title":"Bioinformatics Analysis Identifies <i>ASCL1</i> as the Key Transcription Factor in Hepatocellular Carcinoma Progression.","authors":"Hong-Yan Zhang, Rui-Qing Zong, Fei-Xiang Wu, Yi-Ran Li","doi":"10.1155/2023/3560340","DOIUrl":"10.1155/2023/3560340","url":null,"abstract":"<p><strong>Methods: </strong>Differentially transcription factors (DETFs) were identified from differentially expressed genes (DEGs) in GSE62232 and transcription factors. Then, they were analyzed by regulatory networks, prognostic risk model, and overall survival analyses to identify the key DETF. Combined with the regulatory networks and binding site analysis, the target mRNA of key DETF was determined, and its prognostic value in HCC was evaluated by survival, clinical characteristics analyses, and experiments. Finally, the expressions and functions of the key DETF on the DEmRNAs were investigated in HCC cells.</p><p><strong>Results: </strong>Through multiple bioinformatics analyses, <i>ASCL1</i> was identified as the key DETF, and <i>SLC6A13</i> was predicted to be its target mRNA with the common binding site of CCAGCAACTGGCC, both downregulated in HCC. In survival analysis, high <i>SLC6A13</i> was related to better HCC prognosis, and <i>SLC6A13</i> was differentially expressed in HCC patients with clinical characteristics. Furthermore, cell experiments showed the mRNA expressions of <i>ASCL1</i> and <i>SLC6A13</i> were both reduced in HCC, and their overexpressions suppressed the growth, invasion, and migration of HCC cells. Besides, over-<i>ASCL1</i> could upregulate <i>SLC6A13</i> expression in HCC cells.</p><p><strong>Conclusion: </strong>This study identifies two suppressor genes in HCC progression, <i>ASCL1</i> and <i>SLC6A13</i>, and the key transcription factor <i>ASCL1</i> suppresses HCC progression by targeting <i>SLC6A13</i> mRNA. They are both potential treatment targets and prognostic biomarkers for HCC patients, which provides new clues for HCC research.</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"3560340"},"PeriodicalIF":0.0,"publicationDate":"2023-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9902118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10683624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TREM2 as a Potential Immune-Related Biomarker of Prognosis in Patients with Skin Cutaneous Melanoma Microenvironment.","authors":"Xinlin Zhu, Zhaoxiang Zeng, Min Chen, Xianzhen Chen, Dongying Hu, Weiwei Jiang, Mingwei Du, Tianyang Chen, Tiancheng Chen, Wanqing Liao, Chao Zhang, Ying Qu, Weihua Pan","doi":"10.1155/2023/8101837","DOIUrl":"10.1155/2023/8101837","url":null,"abstract":"Background The skin cutaneous melanoma (SKCM) is a devastating form of skin cancer triggered by genetic and environmental factors, and the incidence of SKCM has rapidly increased in recent years. Immune infiltration of the tumor microenvironment is positively associated with overall survival in many tumors. Triggering receptor expressed on myeloid cells 2 (TREM2) is a transmembrane receptor of the immunoglobulin superfamily and a crucial signaling hub for multiple pathological pathways that mediate immunity. Although numerous evidences suggest a crucial role for TREM2 in tumorigenesis of some tumors, no systematic SKCM analysis of TREM2 is available. Mehods. The relationship between TREM2 expression and diagnostic and prognostic value of SKCM patients via using The Cancer Genome Atlas (TCGA) data. The expression level of TREM2 and clinical characteristic correlation in SKCM patients were assessed by the Wilcoxon rank sum test. The cox regression methods, Kaplan-Meier (KM), and log-rank test were used to assess the impact of TREM2 expression on the overall survival (OS). Furthermore, the Gene Set Enrichment Analysis (GSEA) and TIMER were performed to evaluate the enrichment pathways and potential functions and quantify the immune cell infiltration level for TREM2 expression. Results The TREM2 in SKCM sample expression levels was significantly higher than in normal tissues. Moreover, this expression level of TREM2 was also associated with the BMI of SKCM patients. KM overall survival analysis and OS curve displayed that a high-level TREM2 expression was significantly correlated with a better SKCM prognosis of patients as compared with a low level of TREM2 expression. The GSEA analysis also revealed that TREM2 was associated with immune functions, such as neutrophil activation. Conclusion TREM2 played a crucial role in SKCM, which might be a prognostic biomarker and correlated with immune infifiltrates in SKCM patients.","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"8101837"},"PeriodicalIF":0.0,"publicationDate":"2023-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9897921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10661672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Plasma Proteins Identified via iTRAQ-Based Analysis Serve as Diagnostic Markers of Pancreatic Ductal Adenocarcinoma.","authors":"Xiubing Chen, Xiaomin Liao, Biaolin Zheng, Feng Wang, Feiran Chen, Zhejun Deng, Haixing Jiang, Shanyu Qin","doi":"10.1155/2023/5145152","DOIUrl":"10.1155/2023/5145152","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to identify differentially expressed proteins in the plasma of patients with pancreatic cancer and control subjects, which could serve as potential tumor biomarkers.</p><p><strong>Methods: </strong>Differentially expressed proteins were determined via isostatic labeling and absolute quantification (iTRAQ). Potential protein biomarkers were identified via enzyme-linked immunosorbent assay (ELISA) in 40 patients and 40 control subjects, and those eventually selected were further validated in 40 pancreatic cancer and normal pancreatic tissues.</p><p><strong>Results: </strong>In total, 30 proteins displayed significant differences in expression among which 21 were downregulated and 9 were upregulated compared with the control group. ELISA revealed downregulation of peroxiredoxin-2 (PRDX2) and upregulation of alpha-1-antitrypsin (AAT), Ras-related protein Rab-2B (RAB2B), insulin-like growth factor-binding protein 2 (IGFBP2), Rho-related GTP-binding protein RhoC (RHOC), and prelamin-A/C (LMNA) proteins in 40 other samples of pancreatic cancer. Notably, only AAT, RAB2B, and IGFBP2 levels were consistent with expression patterns obtained with iTRAQ. Moreover, all three proteins displayed a marked increase in pancreatic cancer tissues. Data from ROC curve analysis indicated that the diagnostic ability of AAT, RAB2B, and IGFBP2 combined with carbohydrate antigen 19-9 (CA19-9) for pancreatic cancer was significantly greater than that of the single indexes (area under the curve (AUC): 90% vs. 75% (CA19-9), 76% (AAT), 71% (RAB2B), and 71% (IGFBP2), all <i>P</i> < 0.01).</p><p><strong>Conclusion: </strong>AAT, RAB2B, and IGFBP2 could serve as effective biomarkers to facilitate the early diagnosis of pancreatic cancer.</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"5145152"},"PeriodicalIF":0.0,"publicationDate":"2023-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9883097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10589997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Schisandrin B Alleviates Diabetic Cardiac Autonomic neuropathy Induced by P2X7 Receptor in Superior Cervical Ganglion via NLRP3.","authors":"Zhihua Zhang, Hongmin Guo, Zihui Hu, Congfa Zhou, Qixing Hu, Hao Peng, Gan Tang, Zehao Xiao, Lingzhi Pi, Guilin Li","doi":"10.1155/2023/9956950","DOIUrl":"10.1155/2023/9956950","url":null,"abstract":"<p><p>Diabetic cardiovascular autonomic neuropathy (DCAN) is a common complication of diabetes mellitus which brings about high mortality, high morbidity, and large economic burden to the society. Compensatory tachycardia after myocardial ischemia caused by DCAN can increase myocardial injury and result in more damage to the cardiac function. The inflammation induced by hyperglycemia can increase P2X7 receptor expression in the superior cervical ganglion (SCG), resulting in nerve damage. It is proved that inhibiting the expression of P2X7 receptor at the superior cervical ganglion can ameliorate the nociceptive signaling dysregulation induced by DCAN. However, the effective drug used for decreasing P2X7 receptor expression has not been found. Schisandrin B is a traditional Chinese medicine, which has anti-inflammatory and antioxidant effects. Whether Schisandrin B can decrease the expression of P2X7 receptor in diabetic rats to protect the cardiovascular system was investigated in this study. After diabetic model rats were made, Schisandrin B and shRNA of P2X7 receptor were given to different groups to verify the impact of Schisandrin B on the expression of P2X7 receptor. Pathological blood pressure, heart rate, heart rate variability, and sympathetic nerve discharge were ameliorated after administration of Schisandrin B. Moreover, the upregulated protein level of P2X7 receptor, NLRP3 inflammasomes, and interleukin-1<i>β</i> in diabetic rats were decreased after treatment, which indicates that Schisandrin B can alleviate the chronic inflammation caused by diabetes and decrease the expression levels of P2X7 via NLRP3. These findings suggest that Schisandrin B can be a potential therapeutical agent for DCAN.</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9956950"},"PeriodicalIF":0.0,"publicationDate":"2023-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9845055/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10561731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between Serum Cys C and PTB Cavitation.","authors":"Shumin Tan,&nbsp;Duochi Wu,&nbsp;Yeying Wu,&nbsp;Xing Ren,&nbsp;Jiaxiu Liu,&nbsp;Xiaobin Wei","doi":"10.1155/2023/6465182","DOIUrl":"https://doi.org/10.1155/2023/6465182","url":null,"abstract":"<p><strong>Background: </strong>Cystatin C (Cys C) not only regulates the body's immune defenses but also contributes to tissue degradation and destruction by causing an imbalance between protease and antiprotease in infectious diseases. Is Cys C involved in pulmonary tuberculosis (PTB) infection and cavitation? We therefore conducted a retrospective study on this question to provide a basis for further studies.</p><p><strong>Methods: </strong>Cavitary PTB patients, noncavitary PTB patients, and healthy controls were recruited in our study. Serum Cys C, CRP, BUN, UA, and CR were measured in all subjects, and the Kruskal-Wallis test was used to compare medians of these clinical parameters in different groups. The Spearman rank correlation test was used to determine correlations between variables. In addition, a multivariate analysis using binary logistic regression was used to identify factors associated with PTB cavitation.</p><p><strong>Results: </strong>In our study, elevated serum Cys C levels were found in cavitary PTB patients compared to healthy controls and noncavitary patients (<i>p</i> = 0.022). Serum Cys C levels were statistically correlated with serum BUN and CR concentrations (<i>r</i> = 0.278, <i>p</i> = 0.005; <i>r</i> = 0.281, <i>p</i> = 0.004) in PTB patients. The binary logistic regression analysis showed that elevated serum Cys C levels were correlated with pulmonary cavitation in PTB patients (OR = 1.426, 95% CI: 1.071-1.898).</p><p><strong>Conclusion: </strong>Elevated serum levels of Cys C are associated with pulmonary cavitation in PTB patients.</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"6465182"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10115526/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9390009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Coagulation Dysfunction in Patients with Liver Cirrhosis and Splenomegaly and Its Countermeasures: A Retrospective Study of 1522 Patients.","authors":"Yunfu Lv,&nbsp;Ning Liu,&nbsp;Yejuan Li,&nbsp;Jincai Wu,&nbsp;Jinfang Zheng,&nbsp;Xinqiu Li,&nbsp;Min Zeng","doi":"10.1155/2023/5560560","DOIUrl":"https://doi.org/10.1155/2023/5560560","url":null,"abstract":"<p><strong>Objective: </strong>Patients with cirrhosis and splenomegaly often have coagulation dysfunction which affects treatment and prognosis. This study explores the status, grading, and treatment strategies of coagulation dysfunction in patients with liver cirrhosis and splenomegaly.</p><p><strong>Methods: </strong>A retrospective cohort study was conducted on the clinical data on consecutive patients with cirrhosis and splenomegaly treated at Hainan General Hospital, China, from January 2000 to December 2020. Starting research in January 2022.</p><p><strong>Results: </strong>Among 1522 patients included into this study, 297 (19.5%) patients had normal results in all five coagulation tests (prothrombin time, prothrombin activity, activated partial thromboplastin time, thrombin time, and fibrinogen), and 1225 (80.5%) had coagulation dysfunction in at least one of these tests. There were significant differences (<i>P</i> < 0.05) in treatment efficacy on these patients for three of these five coagulation tests, with the exception of prothrombin activity and thrombin time. When coagulation dysfunction was classified into grades I, II, and III based on scores from the three significant coagulation tests, prothrombin time, activated partial thromboplastin time, and fibrinogen, significant differences in surgical outcomes were found among the three grades of coagulation dysfunction and between grades I and III (<i>P</i> < 0.05). The operative mortality rate in patients with grade III in treating liver cancer, portal hypersplenism, and/or splenomegaly was 6.5%. There was no significant difference between patients with grades I and II (<i>P</i> > 0.05).</p><p><strong>Conclusions: </strong>Approximately, 80% of patients with liver cirrhosis and splenomegaly had coagulation dysfunction. Surgery is feasible for grade I and II patients. For grade III patients, nonsurgical treatment should be given first, and surgery should only be considered when the coagulation function returns to normal or near-normal levels after treatment. This trial is registered with MR-46-22-009299.</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"5560560"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10266912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9657340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: The Correlation between Functional Connectivity of the Primary Somatosensory Cortex and Cervical Spinal Cord Microstructural Injury in Patients with Cervical Spondylotic Myelopathy.","authors":"Disease Markers","doi":"10.1155/2023/9879526","DOIUrl":"https://doi.org/10.1155/2023/9879526","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/2623179.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9879526"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307427/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9737003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: Efficacy of Dapagliflozin in Patients with Diabetes Mellitus Complicated with Coronary Artery Disease and Its Impact on the Vascular Endothelial Function.","authors":"Disease Markers","doi":"10.1155/2023/9807108","DOIUrl":"https://doi.org/10.1155/2023/9807108","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/4829750.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9807108"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10307339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9737011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: RUNX3-Regulated GALNT6 Promotes the Migration and Invasion of Hepatocellular Carcinoma Cells by Mediating O-Glycosylation of MUC1.","authors":"Disease Markers","doi":"10.1155/2023/9872910","DOIUrl":"https://doi.org/10.1155/2023/9872910","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/2959846.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9872910"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371642/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retracted: HSPA5 Inhibitor Meliorate DSS-Induced Colitis through HSPA1A/CHIP.","authors":"Disease Markers","doi":"10.1155/2023/9867919","DOIUrl":"https://doi.org/10.1155/2023/9867919","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/7115181.].</p>","PeriodicalId":11201,"journal":{"name":"Disease Markers","volume":"2023 ","pages":"9867919"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10371480/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10245789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}