International Conference on Photonics and Imaging in Biology and Medicine最新文献_第5页

Optical coherence tomography based projected index computed tomography 基于投影指数计算机断层扫描的光学相干断层扫描

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741436

Zhe Zhou, Z. Ding, Yaliang Yang

引用次数: 0

Dynamic imaging of cerebral blood flow in rat reperfused mini-stroke model using laser speckle temporal contrast analysis 激光散斑时间对比分析大鼠再灌注小卒中模型脑血流动态成像

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741504

Zhen Wang, W. Luo, Pengcheng Li, S. Zeng, Qingming Luo

{"title":"Dynamic imaging of cerebral blood flow in rat reperfused mini-stroke model using laser speckle temporal contrast analysis","authors":"Zhen Wang, W. Luo, Pengcheng Li, S. Zeng, Qingming Luo","doi":"10.1117/12.741504","DOIUrl":"https://doi.org/10.1117/12.741504","url":null,"abstract":"Laser speckle temporal contrast analysis (LSTCA) was used to image the cerebral blood flow (CBF) of ischemic area in reperfused mini-stroke model in rats. Focal cortical ischemia in male Sprague-Dawley rats (n=20) was induced by deliberate ligation of multiple branches of the middle cerebral artery (MCA) together with a nylon ring and the dura. LSTCA was used to monitor the spatio-temporal characteristics of cerebral blood flow dynamics in the rat somatosensory cortex in the ischemic and reperfused stages. The infarction volume was measured by 2, 3, 5- triphenyltetrazolium chloride (TTC) staining 24 hours after reperfusion. The distribution of changes in cerebral blood flow which outlined by the laser speckle imaging represented the relative CBF gradient (21.98±1.96%, 67.2±1.67 %, 107.24±4.71 % of the baseline) from ischemic core, penumbra zone to normal tissue immediately after cortical ischemia, in which a central ischemic core had little or no perfusion surrounded by a penumbral region with reduced perfusion, in addition, we had shown the existence of a surrounding region of hyperemic tissue; Thereafter a postrecanalization hyperperfusion occurred in the same infarct core since 24 hours after reperfusion (242.62±18.52% of the baseline). Histology of the ischemic regions at 24 hours after reperfusion revealed small focal infarcts that were typically 3~4 mm in diameter, approximately equal to the nylon ring in size and position and essentially accordant with the spatial distribution of the ischemic cortex with below 30% residual CBF of the pre-ischemic baseline. It was demonstrated that this technique of LSTCA was easy to implement and availably used to image the spatial and temporal evolution of CBF changes with high resolution in rat reperfused mini-stroke model.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"97 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122076711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast photoacoustic imaging with multi-element linear transducer array system 多单元线性换能器阵列系统的快速光声成像

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741452

Diwu Yang, D. Xing, Huai-min Gu, L. Zeng, L. Xiang, Sihua Yang

引用次数: 0

Dynamic activation of H-Ras induced by low power laser irradiation in living cells 低功率激光辐照诱导活细胞H-Ras的动态活化

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741572

Xuejuan Gao, D. Xing, Yihui Pei, F. Wang

{"title":"Dynamic activation of H-Ras induced by low power laser irradiation in living cells","authors":"Xuejuan Gao, D. Xing, Yihui Pei, F. Wang","doi":"10.1117/12.741572","DOIUrl":"https://doi.org/10.1117/12.741572","url":null,"abstract":"Low-power laser irradiation (LPLI) has been shown to promote cell proliferation in various cell types, yet the mechanism of which has not been fully clarified. Studying the signaling pathways involved in the laser irradiation is important for understanding these processes. The Ras/Raf/MEK/ERK (extracellular-signal-regulated kinase) signaling pathway is a network that governs proliferation, differentiation and cell survival. Recent studies suggest that Ras/Raf signaling pathway is involved in the LPLI-induced cell proliferation, but the dynamic activation of Ras in living cells induced by LPLI has not been reported. In present study, to monitor the dynamic activation of H-Ras after LPLI treatment in living cells in real time, Raichu-Ras reporter was utilized, which was constructed based on fluorescence resonance energy transfer (FRET) technique. Our results show that the dynamic activation of H-Ras at the cell edges of the plasma membrane is monitored during cell proliferation induced by LPLI (0.8 J/cm2) in serum-starved COS-7 cells expressing Raichu-Ras reporter using FRET imaging on laser scanning confocal microscope, and that LPLI causes the redistribution of H-Ras from the cytoplasm to plasma membrane. The same results are observed in EGF treated COS-7 cells. Taken together, LPLI induces the COS-7 cells proliferation by activated Ras.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128495524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Absorption spectrum characteristic of chorophyll derivatives photosensitizer CPD3 叶绿素衍生物光敏剂CPD3的吸收光谱特性

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741634

Fenghua Xiang, Hui Liu, Youshan Lu, Ping Chen, G. Tang

引用次数: 0

Temporal dynamics of blood microcirculation in oral cavity mucous membrane, caused by low-intensity laser irradiation at the wavelength 630 nm 波长630 nm的低强度激光照射对口腔黏膜血液微循环的时间动态影响

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741506

Natalia A. Kharish, Elena V. Safonkina, M. Naumov, S. Ulyanov, N. Bulkina, L. Ostrovskaya, Yulia Osipova, Katia Omelchenko

{"title":"Temporal dynamics of blood microcirculation in oral cavity mucous membrane, caused by low-intensity laser irradiation at the wavelength 630 nm","authors":"Natalia A. Kharish, Elena V. Safonkina, M. Naumov, S. Ulyanov, N. Bulkina, L. Ostrovskaya, Yulia Osipova, Katia Omelchenko","doi":"10.1117/12.741506","DOIUrl":"https://doi.org/10.1117/12.741506","url":null,"abstract":"Influence of laser irradiation on the statement of blood microcirculation in mucous membrane of human oral cavity has been investigated. Speckle methods and Doppler diagnostics have been used for investigation of temporal fluctuation of velocity of blood microcirculation. Pulse waves (low temporal changes of hemodynamics) have been studied by methods of laser photoplethysmography. Influence of coherent light (at the wavelength 630 nm) on the intensity of microcirculation in the capillary net of mucous membrane has been demonstrated directly during the short-time session of laser therapy. It is shown that during the session of laser therapy it is expedient to use first-order frequency-weighted spectral moments of Doppler signal for evaluation of the effects of laser irradiation on the intensity of microcirculation in human oral cavity mucous membrane. Preliminary investigations, involving the objective methods of monitoring the statement of microcirculation, show that coherent light can cause the slight enhancement of intensity of microcirculation during the short sessions of laser therapy. Effect of lasers on the blood saturations is not reliably observed.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130312074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Newly developed optical systems and their potential applications 新开发的光学系统及其潜在应用

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741309

Guoliang Huang, Jiang Zhu, Cheng Deng, Shukuan Xu, Zhonghua Dong, Yang Yang, Xiaoyong Yang, Xianhua Wang, K. Mitchelson, Jing Cheng

引用次数: 0

Quantitative fluorescence resonance energy transfer measurements using microarray technology 定量荧光共振能量转移测量使用微阵列技术

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741482

Jiang Zhu, Cheng Deng, Guoliang Huang, Yang Yang, Shukuan Xu, Zhonghua Dong, Xiaoyong Yang, K. Mitchelson, Jing Cheng

{"title":"Quantitative fluorescence resonance energy transfer measurements using microarray technology","authors":"Jiang Zhu, Cheng Deng, Guoliang Huang, Yang Yang, Shukuan Xu, Zhonghua Dong, Xiaoyong Yang, K. Mitchelson, Jing Cheng","doi":"10.1117/12.741482","DOIUrl":"https://doi.org/10.1117/12.741482","url":null,"abstract":"Fluorescence resonance energy transfer (FRET) has found wide use in structural biology, biochemistry, and cell biology for measuring intra- and inter-molecular distances in the 1-10 nm range and for obtaining quantitative spatial and temporal information about the interaction of proteins, lipids, and DNA. The measurements of distances and interactions are based on the calculation of the fluorescence transfer efficiency using some algorithms to process the acquired images from several different filter sets. However, FRET measurements can suffer from several sources of distortion because of cross talk between donor and acceptor fluorophores. In this paper, we measured the FRET efficiency on glass coverslips using microarray technology and described an algorithm to analyze the FRET data obtained, which is corrected for the cross talk due to spectral overlap of donor and acceptor molecules. Measurement of the interaction of the donor and acceptor, which are mixed together or coupled to the respective 3'-end and 5'-end of a single-strand DNA are shown to document the accuracy of the approach, and allow one to estimate cross talk between the different filter units and to reveal the relationship of the FRET efficiencies of these two samples relative to the donor and acceptor concentrations.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125483384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Noninvasive blood glucose measurement system based on three wavelengths in near-infrared region 基于近红外三波长的无创血糖测量系统

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741454

Yaqin Chen, G. Bai, Jun Xiao, Long Wang, Qingming Luo

{"title":"Noninvasive blood glucose measurement system based on three wavelengths in near-infrared region","authors":"Yaqin Chen, G. Bai, Jun Xiao, Long Wang, Qingming Luo","doi":"10.1117/12.741454","DOIUrl":"https://doi.org/10.1117/12.741454","url":null,"abstract":"Near-infrared region of optical spectrum extends from 0.7 to 2.5μm and can be used for quantitative measurement of organic functional groups, especially C-H, O-H, N-H and C=O. Analyzing sample concentration by near infrared spectroscopy focuses on the design of the optical sensor and the spectral data processing. Noninvasive blood glucose measurement methods using near-infrared spectroscopy usually apply a beam of light to irradiate the blood region of human, and then extract the information of blood glucose from the spectrum. The key is to improve the signal to noise ratio so that very low glucose absorption can be detected. In this paper, according to the absorption of glucose, a noninvasive blood glucose measurement system based on three wavelengths in the near-infrared region was designed. The system included several important parts such as the light source, the optical chopper, the detector and the lock-in amplifier. The three wavelengths were respectively chosen at the signal wavelength 1610nm by glucose absorption peak in the overtone band and the reference wavelengths 1200/1350nm to eliminate the interference effect. The optical probe used an annular light bundle to greatly increase the intensity of incidence light and improve the signal-noise ratio. Two group experiments of glucose aqueous solutions with different concentration interval in the normal human physiological blood glucose range (0-500mg/dL) have been done to evaluate the predictive performance of the system. In these group experiments, the partial least square algorithm was used to predict the glucose concentration. The preliminary results showed when the interval was 100mg/dL, the correlation coefficient (R) was 0.998 and the root mean square error of prediction (RMSEP) was 17.08mg/dL; and when the interval was 20mg/dL, the values of R and RMSEP were 0.959 and 23.22mg/dL, respectively.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134156866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Design and theoretical investigation of a digital x-ray detector with large area and high spatial resolution 大面积高空间分辨率数字x射线探测器的设计与理论研究

International Conference on Photonics and Imaging in Biology and Medicine Pub Date : 2007-05-04 DOI: 10.1117/12.741290

Jianbao Gui, Jinchuan Guo, Qin-lao Yang, Xin Liu, H. Niu

引用次数: 0