International Conference on Photonics and Imaging in Biology and Medicine最新文献

{"title":"Fast 3D imaging of whole organs at cellular resolution by high-throughput muti-scale light sheet microscopy","authors":"Xinyi Guo, Fang Zhao, Wenyang Feng, wentian si, Peng Fei","doi":"10.1117/12.2683139","DOIUrl":"https://doi.org/10.1117/12.2683139","url":null,"abstract":"Rapid 3D imaging of whole organs at cellular resolution is vital to pathological research, but this task remains challenging in light sheet microscopy due to the compromise between axial resolution and field of view. In the meanwhile, needed magnification varies when doing different analysis. Here, we report on a high-throughput multi-scale light sheet microscopy which combines adjustable beam shaping system providing continuous imaging magnification from 1.26X~12.6X, sample holding device for fast sample switching, modulation mask to generate thin optical sectioning and large illumination field, capable of imaging a lung lobe with cellular resolution (~2.8μm), centimeter-scale field of view (1.1cm×0.8cm) within 1 minute. We demonstrate the imaging of hundreds of lung lobes of mice and other large-scale tissue. Using our ultra-high throughput light sheet microscopy with multiple magnification, we are able to quickly get the 3D view of the whole tissue and the statistical data of the volume of tumors, the number tumor cells and so on for further biological analysis.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"12745 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129006351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Toxicological effect evaluation of arsenic exposure in clam Ruditapes philippinarum by using FLIM","authors":"Xiao Peng, Wei Sun, Sihan Wu, Zheng Peng, Zeyu Xiao, Yifeng Deng, Jinchuang Guo, Deliang Yu, Zhen Lu, Wei Yan, Huifeng Wu, Junle Qu","doi":"10.1117/12.2683147","DOIUrl":"https://doi.org/10.1117/12.2683147","url":null,"abstract":"Inorganic arsenic (iAs) is one of the most toxic metalloids which could accumulate in marine species, especially in clams, causing serious ecological risk. Marine clams accumulate high level of iAs in different tissues. Currently, Fluorescence Lifetime Microscopy (FLIM) technique has provided quantitative information in biochemical diagnosis. In this study, we applied FLIM method into analyzing Hematoxylin and eosin (H&E) stained sections of arsenic exposed Ruditapes philippinarum. The clams were exposed under different concentrations of As(Ⅲ) and As(V) for thirty days. Fluorescent images of the H&E stained hepatopancreas tissue were obtained with FLIM system, followed with data analysis for fluorescence lifetime values. The average fluorescence lifetime of sections in the control group was around 250 ps. The average lifetime value in the As(V) group was slightly increased to around 280-300 ps. The average lifetime value in the As(III) group achieved a significant increase to around 340 ps. These results suggested a higher extent of structural change in As(Ⅲ) exposed group than As(V) group. As a result, this work has provided quantitative evaluation standard for the toxicity of marine mollusk based on fluorescence lifetime imaging method.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114084311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoacoustic-photothermal visualisation of gastric tissues based on COMSOL","authors":"Xuke Chu, Feiyang Wang, Chuanhui Ge, Wangbiao Li, Xiaoman Zhang, Shulian Wu, Hui Li","doi":"10.1117/12.2682792","DOIUrl":"https://doi.org/10.1117/12.2682792","url":null,"abstract":"Photoacoustic Imaging (PAI) is a non-invasive imaging technology. It breaks through the limitation of shallow imaging depth of traditional optical imaging and low imaging contrast of acoustic imaging. It has a potential wide application in early cancer detection. PAI combined with endoscopy has great significance in the early diagnosis of gastric tumors. However, imaging just obtains the local location information. It cannot obtain the information of any location visually, especially in the process of photothermal therapy. In this paper, COMSOL was employed to simulate the visual model of photoacoustic photothermal imaging of gastric tissue and tumor. The physical processes of photoacoustic imaging such as photothermal strain-ultrasound generated by pulsed laser in stomach were simulated. A complete photoacoustic model was established through the coupling of each physical field. Firstly, a simplified gastric tissue model was constructed, and physical parameters were set for grid division. Then several physical field modules in COMSOL were coupled. The light distribution, temperature change and photoacoustic signal generated by thermal expansion during the propagation of light in biological tissues were obtained by simulation. The simulation results showed that the distribution of light in gastric tumor was significantly lower than that around the stomach. The tumor absorbs more light energy, resulting in a significantly higher temperature than surrounding stomach tissue. The photoacoustic signal of thermal expansion caused by laser irradiation of biological tissue was obtained. This study is helpful to understand the propagation and interaction of light in normal tissues and gastric tumors. It also provides a certain reference for the research and application of photoacoustic imaging in gastric cancer.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114924811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel vessels feature extraction method in traditional Chinese medicine (TCM）","authors":"Hong Peng, Yilin Zhang, N. Niu, Jiahao Wang, Yiming Liu, Guanjun Wang, Chenyang Xue, Mengxing Huang","doi":"10.1117/12.2679820","DOIUrl":"https://doi.org/10.1117/12.2679820","url":null,"abstract":"In Chinese medicine, eye diagnosis is essential for diagnosis and treatment. However, most current image-processing techniques focus on tongue diagnosis, and most foreign studies on ocular diagnosis focus on segmenting fundus vascular images. Moreover, most of the foreign studies on scleral vessels are focused on identification rather than on TCM discernment. Scleral vessels can significantly characterize the pathological features of the human body’s five internal and six internal organs. Scleral vessels are essential for the objective study of TCM visual diagnosis. However, due to the small size and complex structure of scleral vessels, it is difficult to extract them by existing methods effectively. To achieve more accurate scleral blood vessel extraction, we introduce the residual connection structure and CA-Module attention mechanism in the U2Net1 network to avoid the incompatibility between high-level and low-level features and enhance the information extraction by input fusion and feature extraction of RSU blocks. The experimental results show that Miou achieves an accuracy of 83.3%. The F1-score reaches 91.7%, which is more effective than the existing SOTA fundus vascular segmentation network FR-UNet2 for the experiments. According to the experimental results, Res-U2Net can segment sclerar vessels accurately. In future experiments, we will improve the vessel feature extraction network to increase its accuracy and gradually achieve better results.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133668946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoacoustic imaging: from bench to bedside","authors":"Zhicheng Shao, Jiali Chen, Li Lin","doi":"10.1117/12.2683111","DOIUrl":"https://doi.org/10.1117/12.2683111","url":null,"abstract":"Photoacoustic Imaging (PAI) is an emerging imaging modality with great potential for preclinical research and clinical practice. Uniquely combining optical excitation and acoustic detection, PAI provides a rich contrast mechanism from either endogenous or exogenous chromophores with high spatial resolutions in both optical ballistic and diffusive regimes. This manuscript focuses on the efforts to translate PAI from the bench to the bedside by increasing the imaging depth and speed, providing clinically useful information (i.e., imaging contrast), reducing the device size, and improving the system’s reliability. Assisted by advanced laser systems, ultrasonic arrays, and data acquisition circuits, modern PAI can reveal hematogenous anatomy and functions at high spatiotemporal resolution in small animals and human organs. As a rapidly evolving imaging technology, PAI promises multiple preclinical and clinical applications, attracting growing interest in oncology, dermatology, pediatrics, neuroscience, and orthopedics, among other areas of study.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"1538 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128061380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vessel segmentation and quantification in ultra-wide-field OCTA images","authors":"Xiyao Qiang, Xiangning Wang, Qiang Wu, Guogang Cao, Jiaqing Zhao, Cuixia Dai","doi":"10.1117/12.2683002","DOIUrl":"https://doi.org/10.1117/12.2683002","url":null,"abstract":"Early detection of retinopathy in the periphery of the macula is an important step in preventing severe vision loss. Some morphological parameters about the extensive retina can be obtained through ultra-wide-field OCTA images. Based on small-scale fundus OCTA vessel segmentation, accurate diagnosis can already be obtained by means of deep learning. However, no similar research of segmentation of peripheral blood vessels is reported. In this study, blood vessels of retina were segmented, and blood vessel centerlines were extracted in ultra-wide-field OCTA images. Quantification of the segmented images was performed to explore features of blood vessel. We used a U-shaped neural network that performs well on small samples to cope with the problem of limited data sets. Scale compression and slice segmentation were used to apply the trained network model to vessel segmentation and centerline extraction in ultra-wide-field OCTA images which is of size at 21mm×21mm. Based on the results of the segmentation of blood vessels, the diameter index of blood vessels and vascular tortuousness were calculated, which proved to be associated with some eye diseases. These results and parameters can be helpful for the early screening of some ophthalmic diseases.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"128 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115550771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D visualization and detection of glomeruli in whole mouse kidney","authors":"Yuxin Li, Jia Cao, A. Li, Xiangning Li, Zhao Feng","doi":"10.1117/12.2681039","DOIUrl":"https://doi.org/10.1117/12.2681039","url":null,"abstract":"The kidney is an important organ in the body to excrete metabolic waste, and the glomerulus is an essential structure for the kidney to play a role in blood filtration. Abnormal glomerular numbers are associated with nephropathy or circulatory disease. With the development of imaging technology, mesoscopic optical imaging can obtain whole kidney images at single cell resolution. The detection of glomeruli from images is very crucial for understanding the renal function and studying nephropathy. Existing detection methods cannot balance both accuracy and efficiency, so we proposed a deep learning-based glomeruli detection method. First, we imaged an entire mouse kidney with High-Definition fluorescent Micro-Optical Sectioning Tomography (HD-fMOST) and obtained a three-dimensional (3D) kidney image at cellular resolution. Then, we designed an end-to-end 3D convolutional neural network based on the morphological features of glomeruli in the kidney image, which can directly read 3D images and predict the precise coordinates of glomeruli. We used the acquired kidney dataset to train the network and validated the effect of glomeruli detection. Finally, we applied our approach to detect glomeruli in large-scale mouse kidney. The results showed that the proposed method reached the state-of-the-art level, which is more efficient and accurate compared to similar methods. The proposed method will provide a powerful tool for kidney-related research.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125548402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photoacousto-photothermal effect and temperature monitoring of tissues subjected to graphene oxide","authors":"Chuanhui Ge, Hui Lin, Xiaoman Zhang, Feiyang Wang, Xuke Chu, Ke Li, Hengchang Guo, Shulian Wu","doi":"10.1117/12.2682772","DOIUrl":"https://doi.org/10.1117/12.2682772","url":null,"abstract":"Tissue local temperature information is necessary for guiding treatment parameters in photothermal therapy. Therefore, a temperature monitoring method suitable for the treatment process is needed for monitoring tissue temperature in real time. In this study, a temperature monitoring system based on PID on the photothermal effect of graphene oxide on tissue was proposed. Graphene Oxide (GO) has high photothermal conversion performance and low cytotoxicity under near infrared laser irradiation at 808nm. The photoacoustic imaging system and infrared thermal imager were employed to monitor the effect of GO as a photothermal agent on the photoacoustic signal and temperature of tissues. Firstly, the relationship between the intensity of photoacoustic signal and the temperature of tissues under the action of GO was established. Then, the PID feedback algorithm was applied to monitor and regulate the temperature change of tissues by the intensity of photoacoustic signal, so as to achieve the purpose of photothermal treatment. The results show that GO can enhance the photoacoustic signal of the tissue under laser irradiation and improve the temperature of the irradiated tissue. The system can effectively monitor and regulate the tissue temperature to achieve the therapeutic effect of tumor with little effect on normal tissue.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"167 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117238296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FLIM reveals high salinity resistance in a green algae Chlorella sp. under light and dark conditions","authors":"Zeyu Xiao, Yifeng Deng, Wei Sun, Sihan Wu, Zixin Zhang, Yin-chu Wang, Haipeng Liu, Yijia Zeng, Zhen Lu, Xiao Peng, Huifeng Wu, J. Qu","doi":"10.1117/12.2683124","DOIUrl":"https://doi.org/10.1117/12.2683124","url":null,"abstract":"Chlorella is a unicellular spherical green microalga with alternate colors from blue green to yellowish or red due to different components of innate pigments. Light and salinity are two important environmental factors in Chlorella culture. Light conditions directly affect the growth and biochemical composition of microalgae, while salinity change could influence the pigment composition of Chlorella. Therefore, it has crucial research significance to monitor the response of Chlorella to salinity stress under different light conditions. Recently, Fluorescence Lifetime Imaging Microscopy (FLIM) technology has been widely applied into biological fields, providing fluorescence lifetime values for quantitative analysis. Here, FLIM method was used to observe the autofluorescence of a freshwater microalga, Chlorella sp.. Chlorella cells were treated with a series of salinity concentrations (control sample in normal culture medium, 3S sample with an additional 3× salinity, 7S sample with an additional 7× salinity, respectively) under light (12 h/12 h light/dark cycles) or dark (0 h/24 h light/dark cycles) treatments. After one day, images of the microalgae cells from each group were obtained with FLIM system, followed by an analysis with SPCImage software. The results showed that 3× salinity condition had little effect on Chlorella in both light/dark conditions, suggesting the adaptive capacity of Chlorella to seawater salinity. By contrast, the mean fluorescence lifetime values in 7S samples under light conditions were significantly decreased compared to that of the control. Interestingly, similar lifetime values were observed in 7S samples and the control samples under dark conditions, which indicated a potential high salinity resistance induced by different light/dark conditions. In conclusion, FLIM could work as a fast evaluation method of the physiological status of living Chlorella sp. under different culture conditions in a quantitative way.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"279 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133948730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo monitoring development of zebrafish embryo by optical coherence tomography","authors":"Ke Li, Wangbiao Li, Zaifan Wang, Bingshan Liu, Hui Li, Zhifang Li","doi":"10.1117/12.2683115","DOIUrl":"https://doi.org/10.1117/12.2683115","url":null,"abstract":"Due to the advantages of high sensitivity, high resolution and nondestructive in vivo three-dimensional detection, optical coherence tomography has been widely studied in various fields such as biology, genetics and medicine. Zebrafish is a kind of freshwater fish, whose embryos are easy to reproduce in large numbers and have high transparency for observation. In particular, the genetic homology between zebrafish and human is as high as 70%, which makes zebrafish gradually become an excellent model for studying human development or various serious diseases. In this study, a method for continuous observation of zebrafish embryos using OCT was proposed. In this experiment, the development of zebrafish embryos before hatching (0dpf-3dpf) was continuous observed by OCT, and the proportion of yolk sac to embryo volume was extracted and quantified. The proportion of embryos collected by OCT was compared with the proportion of zebrafish embryos observed by microscope. All experiments were repeated three times. The results show that the method of quantification of zebrafish embryo development by OCT can not only observe the internal development structure of the embryo, but also calculate the volume proportion of embryo development more accurately than microscope. This method provides a more rapid and precise important means for early clinical judgment of embryo development.","PeriodicalId":110373,"journal":{"name":"International Conference on Photonics and Imaging in Biology and Medicine","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121879645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}