Dental Journal (Majalah Kedokteran Gigi)最新文献_第4页

The Effect of Physical-Psychological and Psychological Stress on Blood Glucose Levels (In vivo Study) 生理-心理和心理应激对血糖水平的影响(体内研究)

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-06-09 DOI: 10.18196/di.v12i1.13945

Agustin Wulan Suci Dharmayanti, Erfin Ramadana Pratama, B. Kusumawardani, Z. Hamzah, S. Suhartini, T. Indriana, Dwi Warna Aju Fatmawati

{"title":"The Effect of Physical-Psychological and Psychological Stress on Blood Glucose Levels (In vivo Study)","authors":"Agustin Wulan Suci Dharmayanti, Erfin Ramadana Pratama, B. Kusumawardani, Z. Hamzah, S. Suhartini, T. Indriana, Dwi Warna Aju Fatmawati","doi":"10.18196/di.v12i1.13945","DOIUrl":"https://doi.org/10.18196/di.v12i1.13945","url":null,"abstract":"Stress is a condition that burdens a person and affects homeostasis resulting in health problems, including dental and oral health. Stress can be caused by physical, chemical, or psychological stressors. Stressors cause an increase in blood glucose levels due to an increase in the stress hormone cortisol. This study aims to determine the effect of physical-psychological and psychological stress induced by electrical foot shock on blood glucose levels in rats. This research is an experimental laboratory study with a post-test control group design using white male rats as animal stress models. Animal models were divided into six groups, namely physical-psychological stress for 7 days (PPS1), 14 days (PPS2), and 28 days (PPS3); and psychological stress for 7 days (PS1), 14 days (PS2), and 28 days (PS3). The animal models were placed in the experimental box with electrical foot shock. The physical-psychological stress treatment was to flow an electric current of 48 V, 0.5 Hz, 2 mA for 30 minutes daily. In the psychological stress treatment, the animal models were placed without an electric shock, but the animal models were placed together, given an electric shock stressor separated by transparent plastic with a thickness of 5 cm and foam at the bottom of the cage (insulator). The results showed that although there was no effect of electrical foot shock on blood glucose levels in rats experiencing physical-psychological stress for 7, 14, and 28 exposures (p0.05), electrical foot shock affected blood glucose levels in rats with psychological stress (p0.05). It can be concluded that physical-psychological and psychological stress induced by electrical foot shock affected blood glucose levels.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86030156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Penaeus monodon Shell Powder Extract as A Candidate for Dental Material Restoration 白对虾壳粉末提取物作为牙体修复材料的候选材料

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-06-09 DOI: 10.18196/di.v12i1.16299

M. Rizal, Widyasri Prananingrum, Dian W. Damaiyanti

{"title":"Penaeus monodon Shell Powder Extract as A Candidate for Dental Material Restoration","authors":"M. Rizal, Widyasri Prananingrum, Dian W. Damaiyanti","doi":"10.18196/di.v12i1.16299","DOIUrl":"https://doi.org/10.18196/di.v12i1.16299","url":null,"abstract":"The prevalence of dental caries in Indonesia is still high. One of the dental caries treatments is direct restoration. Biomaterial, often used as a restorative material, is glass ionomer cement which has a silica content of 35%. Silica is found in Penaeus monodon shells which have been a waste of production. This research aims to study the characteristic of Penaeus monodon shell powder extracts as candidates for dental materials restoration. The research sample used was Penaeus monodon shell powder extract which was then carried out by a depigmentation process by mixing acetone 1:10 (w/v) ≤ 20 hours. Samples were tested with the XRD test (x-ray diffraction). SEM assessment was performed to evaluate the morphology of the particle. Data were analyzed using Mann-Whitney. The result demonstrated a significant silica content in Penaeus monodon shell powder extract before and after the depigmentation process with a p-value of 0,00 (p0,05). The depigmentation process can decrease silica content in Penaeus monodon shell powder extract. There was a different comparison of silica content in Penaeus monodon shell powder extract before and after depigmentation as a candidate for dental materials restoration.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"92 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86973689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pengaruh ekstrak biji pepaya (Carica papaya Linn) terhadap tingkat inflamasi gingivitis (studi in vivo pada Rattus norvegicus) 木瓜种子提取物（Carica papaya Linn）对炎症性牙龈炎水平的影响（在鼠体内进行的研究）

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-04-09 DOI: 10.22146/mkgk.83698

Suryono Suryono, Muhammad Reza Pahlevi, Nisaul Afifah, Prayitno Prayitno

{"title":"Pengaruh ekstrak biji pepaya (Carica papaya Linn) terhadap tingkat inflamasi gingivitis (studi in vivo pada Rattus norvegicus)","authors":"Suryono Suryono, Muhammad Reza Pahlevi, Nisaul Afifah, Prayitno Prayitno","doi":"10.22146/mkgk.83698","DOIUrl":"https://doi.org/10.22146/mkgk.83698","url":null,"abstract":"Gingivitis adalah peradangan gingiva yang disebabkan oleh akumulasi plak subgingiva. Plak akan meningkatkan aktivitas sel fagosit dan mediator inflamasi. Infiltrasi sel inflamasi terutama neutrofil polimorfonuklear akan menyebabkan inflamasi yang dapat diamati secara klinis. Indeks gingiva menunjukkan derajat inflamasi pada gingivitis. Penurunan indeks gingiva dapat disimpulkan sebagai penyembuhan peradangan. Pengobatan gingivitis adalah scaling, root planning, dan terapi obat anti inflamasi non steroid (NSAID). Terapi menggunakan NSAID merupakan upaya untuk memodulasi respon host pada inflamasi. Biji pepaya dapat digunakan untuk memodulasi respon inang karena mengandung flavonoid, saponin, dan polifenol yang memiliki efek antiinflamasi, antibakteri dan antioksidan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak biji pepaya terhadap derajat inflamasi pada gingivitis tikus wistar. Tiga puluh ekor tikus wistar dibagi menjadi 5 kelompok yaitu kontrol negatif, kontrol positif, dan tiga kelompok ekstrak biji pepaya dengan konsentrasi 0,2%, 2% dan 20%. Gingivitis diinduksi dengan menempatkan ligatur sutera retentif plak pada gigi seri mandibula dan Aggregatibacter actinomycetemcomitans inokulasi sulkus gingiva. Ekstrak diterapkan dua kali sehari. Tikus didekapitasi pada hari kedua dan kelima kemudian dibuat preparat histologi fragmen gingiva. Perbedaan yang signifikan diamati pada indeks gingiva antara kelompok perlakuan dan kontrol negatif. Penelitian ini juga menemukan penurunan indeks gingiva setelah perawatan dengan ekstrak biji pepaya. Kemudian disimpulkan bahwa ekstrak biji pepaya dapat menghambat inflamasi dan menurunkan derajat inflamasi pada gingivitis tikus wistar.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135648684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Changes in the corrosion rate and microstructure of beta titanium wire using kiwi peel extract 猕猴桃皮提取物对β钛丝腐蚀速率和组织的影响

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-03-15 DOI: 10.20473/j.djmkg.v56.i2.p122-126

Hilda Fitria Lubis, Hanifa Natarisya

{"title":"Changes in the corrosion rate and microstructure of beta titanium wire using kiwi peel extract","authors":"Hilda Fitria Lubis, Hanifa Natarisya","doi":"10.20473/j.djmkg.v56.i2.p122-126","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p122-126","url":null,"abstract":"Background: Beta titanium orthodontic wire is known to have good corrosion resistance but is weak in acidic environments, which advance the corrosion rate. One natural inhibitor that can be used to decrease corrosion is kiwi peel extract, which has a high antioxidant level. Purpose: This study aims to examine the ability of the extract to decrease the corrosion rate and microstructural changes of beta titanium at an acidic pH (pH 5). Methods: The samples used were beta titanium with a diameter of 0.016 x 0.022 in and a length of 6 cm. A total of 28 samples (n=28) were divided into four groups—a control group immersed in pH 5 artificial saliva and three treatment groups immersed in kiwi peel extract at concentrations of 400, 500, and 600 ppm (n=7), respectively. The samples were immersed for seven days at 37oC in an incubator. The corrosion rate was tested using the weight-loss method and microstructure change was analyzed using a scanning electron microscope (SEM). Results: One-way ANOVA showed that there are significant differences in corrosion rates between beta titanium immersed in artificial saliva and beta titanium immersed in kiwi peel extract with p=0.01 (p<0.05). SEM analysis results showed that the group with the least surface changes was the one immersed in 400 ppm of kiwi peel extract. Conclusion: Weight-loss and SEM methods show similar results. Kiwi peel extract proved to decrease the corrosion rate and changes in the microstructure of the wire most effectively at a concentration of 400 ppm.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"280 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72531517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Degradation of Fusobacterium nucleatum biofilm and quantity of reactive oxygen species due to a combination of photodynamic therapy and 2.5% sodium hypochlorite 光动力疗法与2.5%次氯酸钠联合作用对核梭杆菌生物膜降解及活性氧含量的影响

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-03-15 DOI: 10.20473/j.djmkg.v56.i2.p132-138

N. Zubaidah, Sukaton, S. Kunarti, Meidi Kurnia Ariani, Dawailatur Rahman Setiady, D. M. Lashari

{"title":"Degradation of Fusobacterium nucleatum biofilm and quantity of reactive oxygen species due to a combination of photodynamic therapy and 2.5% sodium hypochlorite","authors":"N. Zubaidah, Sukaton, S. Kunarti, Meidi Kurnia Ariani, Dawailatur Rahman Setiady, D. M. Lashari","doi":"10.20473/j.djmkg.v56.i2.p132-138","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p132-138","url":null,"abstract":"Background: The persistence of microorganisms in the root canal system is one of the leading causes of root canal treatment failure. Biofilms of putative pathogens hidden inside dentin tubules and other root canal ramifications may limit current disinfection protocols. Photodynamic therapy (PDT) with a wavelength of 628 nm can be used as an antimicrobial strategy that uses low-power laser energy to activate a non-toxic photosensitizer to produce singlet oxygen with the ability to kill microorganisms in root canals. Fusobacterium nucleatum was used because this bacterium is one of the bacteria involved in root canal infection. Purpose: The aim of this study was to compare the bactericidal efficacy of sodium hypochlorite (NaOCl) 2.5%, PDT, and a combination of PDT and NaOCl 2.5% against Fusobacterium nucleatum. Methods: Mature biofilm Fusobacterium nucleatum was divided into four groups according to the protocol of decontamination: K1 (negative control – biofilm), K2 (NaOCl 2.5%), K3 (PDT), and K4 (NaOCl 2.5% + PDT). Biofilm degradation was observed using optical density (OD) at 570 nm using a microplate reader. A reactive oxygen species quantity check was carried out using a nitroblue tetrazolium test, and OD observation was done with a microplate reader at 540 nm. Results: Group 4 (NaOCl 2.5% + PDT) showed more biofilm bacteria elimination than the other groups. Conclusion: A combination of PDT and NaOCl 2.5% can be considered an effective protocol for the elimination of Fusobacterium nucleatum. There is a potentiation relationship between NaOCl 2.5% and PDT FotoSan. Biofilm degradation occurs because of the effect of antibacterial NaOCl 2.5% and the irradiation effect of the Toluidine blue O photosensitizer.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85133809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The relationship between tooth loss and pre-elderly nutritional status and quality of life 牙齿脱落与老年前营养状况及生活质量的关系

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-03-15 DOI: 10.20473/j.djmkg.v56.i2.p80-86

Widiya Ulfa, Rosa Amalia, A. Santoso

{"title":"The relationship between tooth loss and pre-elderly nutritional status and quality of life","authors":"Widiya Ulfa, Rosa Amalia, A. Santoso","doi":"10.20473/j.djmkg.v56.i2.p80-86","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p80-86","url":null,"abstract":"Background: Tooth loss leads to decreased masticatory function, which affects nutritional intake. Tooth loss accompanied by poor nutritional status affects quality of life both physically and psychosocially. Purpose: This study was conducted to identify the relationship between number of teeth lost due to nutritional status and quality of life. Methods: A study conducted at Gulai Bancah Primary Health Care in Bukittinggi. Participants were recruited using a non-probability purposive sampling technique. 178 pre-elderlies aged between 45 and 59 years took part in this study. Tooth loss was reported in the dental examination format while nutritional status and quality of life pertaining to dental and oral health were determined using the Mini Nutritional Assessment (MNA) and the Geriatric Oral Health Assessment Index (GOHAI). Pearson correlation and path analysis were utilized to analyze the data. Results: The Pearson correlation test indicated a moderate correlation between tooth loss and nutritional status (r = -0.549) and a strong correlation between the number tooth lost and quality of life (r = -0.742). Pathway analysis confirmed that the direct effect value (beta coefficient = 0.552) was greater than the indirect effect value (beta coefficient = 0.189). The findings indicated that, indirectly, the amount of tooth loss mediated with nutritional status had no significant relationship to quality of life. Conclusion: An increase in tooth loss leads to pre-elderly poor nutritional status and quality of life. However, nutritional status is not an intermediate factor between the number of teeth lost and pre-elderly quality of life.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78655072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using the Demirjian method for estimating the dental age of children in Surabaya, Indonesia 使用Demirjian方法估计印度尼西亚泗水儿童的牙齿年龄

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-03-15 DOI: 10.20473/j.djmkg.v56.i2.p87-91

B. F. Prakoeswa, Arofi Kurniawan, An’nisaa Chusida, B. N. Rizky, Anugerah I'zaaz Darmawan, Andini Kamilia Nur Aisyah, Aspalilah Alias

引用次数: 0

Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation 牙槽保存用羟基磷灰石蟹壳BSP和DMP1的评价

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-03-15 DOI: 10.20473/j.djmkg.v56.i2.p104-108

Michael Josef Kridanto Kamadjaja, Sherman Salim, Wiwik Herawati Waluyo, T. Noor

{"title":"Evaluation of BSP and DMP1 in hydroxyapatite crab shells used for dental socket preservation","authors":"Michael Josef Kridanto Kamadjaja, Sherman Salim, Wiwik Herawati Waluyo, T. Noor","doi":"10.20473/j.djmkg.v56.i2.p104-108","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p104-108","url":null,"abstract":"Background: Bone resorption due to tooth extraction leads to unpredictable bone volume for future prosthetics. Crab shells were promoted as a solution to prevent bone resorption, along with an effort to reduce biological waste. Purpose: This study aimed to analyze the expression of bone sialoprotein (BSP) and dentine matrix protein-1 (DMP1) in the wound healing process in tooth-extraction sockets after applying a crab shell-derived hydroxyapatite scaffold. Methods: The subjects (28 Cavia cobaya) were divided into control and treatment groups. The control group was left untreated, while the treatment group received a hydroxyapatite scaffold of Portunus pelagicus shell in the tooth socket. The expression of BSP and DMP1 was determined by immunohistochemical staining on days 7 and 14. One-way analysis of variance and Tukey’s honest significance difference test were used to find the groups with the most significant difference. Results: The highest mean expression of BSP and DMP1 was in the day 14 treatment group, while the lowest was in the day 7 control group. Conclusion: Administering hydroxyapatite scaffold derived from the Portunus pelagicus shell to the post-extraction sockets increased the expression of both BSP and DMP1.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73504916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Inhibitory test of andaliman (Zanthoxylum achantopodium DC) extract mouthwash against dental plaque bacteria 安达里曼(Zanthoxylum achantopodium DC)提取物漱口水对牙菌斑细菌的抑制试验

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-03-15 DOI: 10.20473/j.djmkg.v56.i2.p92-97

Martina Amalia, Priscillia Sekar Yosuana, Iqlima Salsabila binti Mohammad, Filya Suri Risky Nababan, Zulkarnain, Pitu Wulandari, A. Nasution, Armia Syahputra

{"title":"Inhibitory test of andaliman (Zanthoxylum achantopodium DC) extract mouthwash against dental plaque bacteria","authors":"Martina Amalia, Priscillia Sekar Yosuana, Iqlima Salsabila binti Mohammad, Filya Suri Risky Nababan, Zulkarnain, Pitu Wulandari, A. Nasution, Armia Syahputra","doi":"10.20473/j.djmkg.v56.i2.p92-97","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p92-97","url":null,"abstract":"Background: Andaliman (Zanthoxylum achantopodium DC) is an endemic plant that is found in the province of Sumatera Utara, Indonesia. It contains secondary metabolites, such as alkaloids, flavonoids, glycosides, saponins, tannins, and triterpenoids/steroids, which can potentially be used as a mouthwash. Streptococcus sanguinis and Staphylococcus aureus are the primary colonizing bacteria in plaque formation. Bacterial plaque is known to be the main cause of periodontal disease but can be controlled mechanically and chemically using mouthwash. Purpose: To determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of andaliman extract mouthwash (2%, 4%, 8%) against Streptococcus sanguinis ATCC®10556™ and Staphylococcus aureus ATCC® 25923™. Methods: This is a laboratory study with a post-test control-only design. The sample consists of andaliman extract mouthwash (2%, 4%, 8%), a positive control (chlorhexidine gluconate 0.2%), and a negative control (mouthwash formulation without andaliman extract) with three repetitions for each group. Data were analyzed with the one-way ANOVA test and post hoc LSD test. Results: The andaliman extract mouthwash with concentrations of 2%, 4%, and 8% significantly reduced the number of Streptococcus sanguinis and Staphylococcus aureus colonies (p<0.05), and there was a significant difference in the andaliman extract mouthwash with concentrations of 2%, 4%, and 8% compared to the negative control. Conclusion: Andaliman extract mouthwash with a concentration of 8% was more effective in inhibiting Streptococcus sanguinis growth than Staphylococcus aureus. The MIC values for both bacteria were 2%, but the study could not determine the MBC value.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90842213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro 印楝叶乙醇提取物对粪肠球菌体外抗菌活性研究

Dental Journal (Majalah Kedokteran Gigi) Pub Date : 2023-03-15 DOI: 10.20473/j.djmkg.v56.i2.p98-103

S. Suhartono, C. Soraya, Putri Shabira

{"title":"Antibiofilm activity of neem leaf (Azadirachta indica A. Juss) ethanolic extracts against Enterococcus faecalis in vitro","authors":"S. Suhartono, C. Soraya, Putri Shabira","doi":"10.20473/j.djmkg.v56.i2.p98-103","DOIUrl":"https://doi.org/10.20473/j.djmkg.v56.i2.p98-103","url":null,"abstract":"Background: Enterococcus faecalis commonly infects root canals by forming a biofilm. Extracts from neem leaves (Azadirachta indica A. Juss) have been shown to have antibacterial properties, indicating their potential in preventing or treating biofilm formation caused by bacteria. Purpose: This study aims to investigate the phytochemical compounds present in neem leaves (Azadirachta indica A. Juss) and establish the concentration of ethanol-based neem leaf extract that can effectively inhibit the in vitro growth of Enterococcus faecalis biofilm. Methods: This study employed the maceration technique for extraction, gas chromatography mass spectroscopy for the analysis of plant chemicals, and a microtiter plate assay for measuring biofilm formation with treatment concentrations of 6.25%, 12.5%, 25%, 50%, and 75%, with a positive control of 0.2% chlorhexidine. Results: A phytochemical analysis revealed that the ethanol extract of neem leaves contained 22 different metabolites, mainly terpenoids and fatty acids. The extract demonstrated antibiofilm activity only at a concentration of 12.5% with an average biofilm inhibition of 36.85%. However, lower concentrations of 6.25%, 25%, 50%, and 75% had the opposite effect, promoting biofilm formation in Enterococcus faecalis. Conclusion: Phytochemical metabolite contained in the ethanolic extracts of neem leaves might contribute a promising agent in treating a biofilm-mediated root canal infection of Enterococcus faecalis.","PeriodicalId":11034,"journal":{"name":"Dental Journal (Majalah Kedokteran Gigi)","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80359656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1