Cytogenetics and cell genetics最新文献_第2页

Genomic organization of the human ubiquitin-conjugating enzyme gene, UBE2L6 on chromosome 11q12. 11q12染色体上人类泛素结合酶基因UBE2L6的基因组组织。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015593

H C Ardley, S A Rose, N Tan, J P Leek, A F Markham, P A Robinson

引用次数: 10

DRM/GREMLIN (CKTSF1B1) maps to human chromosome 15 and is highly expressed in adult and fetal brain. DRM/GREMLIN (CKTSF1B1)定位于人类第15号染色体，在成人和胎儿大脑中高度表达。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015568

L Z Topol, W S Modi, S Koochekpour, D G Blair

{"title":"DRM/GREMLIN (CKTSF1B1) maps to human chromosome 15 and is highly expressed in adult and fetal brain.","authors":"L Z Topol, W S Modi, S Koochekpour, D G Blair","doi":"10.1159/000015568","DOIUrl":"https://doi.org/10.1159/000015568","url":null,"abstract":"We have mapped and characterized the human homolog of Drm/Gremlin (CKTFS1B1), a member of a family of BMP antagonists that have been linked to both developmental and transformation-related functions. By screening a human cDNA library, we isolated a 3.3-kb cDNA containing the 552-bp region encoding the human DRM protein. CKTFS1B1 was localized on human chromosome 15q13--> q15 by somatic cell hybrid analysis and, more precisely, using radiation hybrids, to a region of markers linked to SGNE1, secretory granule neuroendocrine protein 1 and RYR3, the ryanodyne receptor 3. Northern blot analysis showed the presence of a single DRM-specific mRNA expressed in different human tissues, including brain, ovary, intestine and colon. In the brain, DRM expression is associated with the region localized around the internal capsule in the large subcortical nuclei. DRM appears to be predominantly expressed in normal cells and tissues, including normal neurons, astrocytes and fibroblasts. Interestingly, we detected DRM expression in normal cells obtained from several patients, but not in tumor cell lines established from the same patients. The data suggest that down-regulation of DRM is associated with tumor progression, and support the hypothesis that human DRM may play an important role during both neuroembryological development and carcinogenesis.","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015568","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21737097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 60

Cloning, structural organization, and chromosomal assignment of the porcine c-fos proto-oncogene, FOS. 猪c-fos原癌基因的克隆、结构组织和染色体分配。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015565

G Reiner, L Heinricy, B Brenig, H Geldermann, V Dzapo

引用次数: 11

Localization of the human anterior gradient-2 gene (AGR2) to chromosome band 7p21.3 by radiation hybrid mapping and fluorescencein situ hybridisation. 人类前梯度-2基因(AGR2)在染色体7p21.3带的辐射杂交定位和荧光原位杂交。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015594

E Petek, C Windpassinger, H Egger, P M Kroisel, K Wagner

引用次数: 29

Structure and chromosome location of Smtn, the mouse smoothelin gene. 小鼠平滑素基因Smtn的结构和染色体定位。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015619

S Rensen, G Merkx, P Doevendans, A Geurts Van Kessel, G van Eys

引用次数: 12

Assignment of interleukin-1 receptor, type I (IL1R1) to bovine chromosome band 11q12 by in situ hybridization. 白细胞介素-1受体I型(IL1R1)在牛染色体带11q12上的原位杂交。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015605

B Castiglioni, S Comincini, M G Foti, L Ferretti

{"title":"Assignment of interleukin-1 receptor, type I (IL1R1) to bovine chromosome band 11q12 by in situ hybridization.","authors":"B Castiglioni, S Comincini, M G Foti, L Ferretti","doi":"10.1159/000015605","DOIUrl":"https://doi.org/10.1159/000015605","url":null,"abstract":"Interleukin-1 (IL-1) is an inflammatory cytokine involved in inflammation and immune responses (Dinarello, 1988). The IL-1 family is complex, with three ligands (IL-1·, IL-1ß and the IL-1 receptor antagonist) and two receptors (type I, IL-1R1 and type II, IL-1R2). IL-1R1, present in low numbers on most cells, is the predominant receptor on T-cells and fibroblasts (Sims et al., 1988). IL-1R2 is mainly expressed in neutrophils, B cells and monocytes (McMahan et al., 1991). The human and murine IL-1 receptors are transcribed from separate genes and have been assigned to HSA2 and to MMU1, respectively (Copeland et al., 1991). The bovine IL-1 receptor subtypes have been mapped to BTA11 by linkage analysis (Yoo et al., 1994; Kappes et al., 1997). ZOO-FISH data (Solinas-Toldo et al., 1995) indicate that most of HSA 2q corresponds to BTA 2q12→q44. On the contrary, IL1B (HSA 2q14), is the first locus beyond a breakpoint proximal to PROC (HSA 2q13→ q14) that defines a region of synteny with BTA11 (Lopez-Corrales et al., 1998). Our data assign IL1R1 to BTA 11q12, at a greater distance from IL1B (BTA 11q21→q23) compared to man where the genes are placed in the same chromosomal subregion (HSA 2q12 and 2q14 respectively). Fig. 1. Chromosomal localisation of the bovine IL-1R1 gene. (a) Partial metaphase spread showing a typical FISH with the IL1R1 probe on BTA 11q12. (b) Ideogram of BTA11 showing the position of IL1R1.","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015605","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21800887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1-->q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32-->q34.1 with radiation hybrid mapping. 将人线粒体翻译释放因子1 (MTRF1)定位到染色体13q14.1- >q14.3，将人线粒体核糖体再循环因子(MRRF)定位到染色体9q32- >q34.1。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015494

L L Hansen, R Jørgensen, J Justesen

{"title":"Assignment of the human mitochondrial translational release factor 1 (MTRF1) to chromosome 13q14.1-->q14.3 and of the human mitochondrial ribosome recycling factor (MRRF) to chromosome 9q32-->q34.1 with radiation hybrid mapping.","authors":"L L Hansen, R Jørgensen, J Justesen","doi":"10.1159/000015494","DOIUrl":"https://doi.org/10.1159/000015494","url":null,"abstract":"The human mitochondrial genome is transcribed and translated by gene products encoded by the nuclear genome. The termination of peptide chain synthesis in mitochondria is controlled by release factor 1 (mRF1) which is supposed to read the stop codon. Errors in termination due to a defect or missing mRF1 might lead to carboxy-terminal extensions of the mitochondrial encoded proteins with consequences for energy metabolism and apoptosis via cytochrome C. The release factor has been identified and partly characterized (Zhang and Spremulli, 1998) (LocusLink 9617) as highly homologous to the bacterial release factor 1 (RF1) and less homologous to the cytoplasmic RF1 (ETF1, LocusLink 2107). The ribosome recycling factor is a nuclear encoded mitochondrial protein, which is necessary for recycling of ribosomes after the termination of a peptide chain catalyzed by the mitochondrial translational release factor 1 (MTRF1). The factor is a GTP binding protein with high homology to the bacterial RRF (Zhang and Spremulli, 1998) (LocusLink 10304). There is no known cytoplasmic homologue to mRRF. Materials and methods","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015494","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21622999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Five human genes encoding F-box proteins: chromosome mapping and analysis in human tumors. 编码F-box蛋白的5个人类基因:人类肿瘤中的染色体定位和分析。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015532

D S Chiaur, S Murthy, C Cenciarelli, W Parks, M Loda, G Inghirami, D Demetrick, M Pagano

{"title":"Five human genes encoding F-box proteins: chromosome mapping and analysis in human tumors.","authors":"D S Chiaur, S Murthy, C Cenciarelli, W Parks, M Loda, G Inghirami, D Demetrick, M Pagano","doi":"10.1159/000015532","DOIUrl":"https://doi.org/10.1159/000015532","url":null,"abstract":"Members of the F-box protein (Fbp) family are characterized by an approximately 40 amino acid F-box motif. SCF complexes (formed by Skp1, cullin, and one of many Fbps) act as protein-ubiquitin ligases that control the G(1)/S transition of the eukaryotic cell cycle. The substrate specificity of SCF complexes is determined by the presence of different Fbp subunits that recruit specific substrates for ubiquitination. Unchecked degradation of cellular regulatory proteins has been observed in certain tumors and it is possible that deregulated ubiquitin ligases play a role in the altered degradation of cell cycle regulators. We have recently identified a family of human Fbps. As a first step aimed at determining if FBP genes could be involved in human neoplasia, we have mapped the chromosome positions of 5 FBP genes by fluorescence in situ hybridization (FISH) to 10q24 (BTRC alias beta-TRCP/FBW1a), 9q34 (FBXW2 alias FBW2), 13q22 (FBXL3A alias FBL3a), 5p12 (FBXO4 alias FBX4) and 6q25-->q26 (FBXO5 alias FBX5). Since most of these are chromosomal loci frequently altered in tumors, we have screened 42 human tumor cell lines and 48 human tumor samples by Southern hybridization and FISH. While no gross alterations of the genes encoding beta-Trcp/Fbw1a, Fbw2, Fbx4 and Fbx5 were found, heterozygous deletion of the FBXL3A gene was found in four of 13 small cell carcinoma cell lines. This is the first evaluation of genes encoding Fbps in human tumors.","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015532","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21673212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Assignment of the human metabotropic glutamate receptor gene GRM4 to chromosome 6 band p21.3 by radiation hybrid mapping. 人类代谢性谷氨酸受体基因GRM4在6号染色体p21.3带的定位

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015551

A Barbon, S Ferraboli, S Barlati

引用次数: 7

Assignment of the kinin receptor B1 and B2 genes (Bdkrb1 and Bdkrb2) to rat chromosome 6q3.2 by FISH and radiation hybrid mapping. 利用FISH和辐射杂交定位将激肽受体B1和B2基因(Bdkrb1和Bdkrb2)定位到大鼠染色体6q3.2上。

Cytogenetics and cell genetics Pub Date : 2000-01-01 DOI: 10.1159/000015561

C Gösele, F Grützner, J B Pesquero, J A Silva, T Junge, D Ganten, M Bader, M Knoblauch

{"title":"Assignment of the kinin receptor B1 and B2 genes (Bdkrb1 and Bdkrb2) to rat chromosome 6q3.2 by FISH and radiation hybrid mapping.","authors":"C Gösele, F Grützner, J B Pesquero, J A Silva, T Junge, D Ganten, M Bader, M Knoblauch","doi":"10.1159/000015561","DOIUrl":"https://doi.org/10.1159/000015561","url":null,"abstract":"Kinins are a family of peptide hormones involved in physiological and pathophysiological processes such as smooth muscle contraction, nociception and inflammation (Bhoola et al., 1992). Based on their pharmacological properties two mammalian kinin receptor subtypes, B1 and B2, have been identified and the genes for both subtypes have been cloned from human, rat and mice (for review see Pesquero and Bader, 1998). While the B2 receptor is constitutively expressed in a variety of tissues, the B1 receptor is exceptional in being upregulated following tissue injury and inflammation by the action of cytokines (Marceau et al., 1998). Mice lacking B2 receptors show hypertension, salt sensitivity, and altered nociceptive responses (Borkowski et al., 1995; for review see Pesquero and Bader, 1998). B1-receptor deficient animals exhibit hypoalgesia and altered inflammatory responses (Bader et al., manuscript submitted). The B1 and B2-receptor genes have been assigned to human chromosome 14q32 (Ma et al., 1994; Chai et al., 1996). The B2-receptor gene has been mapped to the homologous region on mouse chromosome 12 (Taketo et al., 1995). Materials and methods","PeriodicalId":10982,"journal":{"name":"Cytogenetics and cell genetics","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1159/000015561","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21737196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4