Current stem cell research & therapy最新文献_第7页

Advances in iPSC Technology in Neural Disease Modeling, Drug Screening, and Therapy. iPSC 技术在神经疾病建模、药物筛选和治疗方面的进展。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X18666230608105703

Sihan Dai, Linhui Qiu, Vishnu Priya Veeraraghavan, Chia-Lin Sheu, Ullas Mony

引用次数: 0

Mesenchymal Stem Cell Therapy for Treating the Underlying Causes of Diabetes Mellitus and Its Consequences. 用于治疗糖尿病根本原因及其后果的间充质干细胞疗法。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X18666230411111320

Diana Esquivel, Rangnath Mishra, Anand Srivastava

{"title":"Mesenchymal Stem Cell Therapy for Treating the Underlying Causes of Diabetes Mellitus and Its Consequences.","authors":"Diana Esquivel, Rangnath Mishra, Anand Srivastava","doi":"10.2174/1574888X18666230411111320","DOIUrl":"10.2174/1574888X18666230411111320","url":null,"abstract":"Diabetes mellitus (DM) is a multifaceted pathological condition, which at present is being considered an epidemic disease keeping the rampant rate of its increase in almost all population groups of the world in consideration. Out of the two types of DM described, T1D is characterized as an autoimmune condition that leads to the destruction of pancreatic β-cells by macrophages and T-cells, thereby, adversely affecting the production of insulin. On the other hand, T2D, often caused by insulin resistance, is commonly related to unhealthy habits, and therefore, it can be prevented in most cases. In both of the conditions, high levels of proinflammatory cytokines like IL-6, TNF-α, and INF-ƴ, lead to chronic inflammation, and elevated oxidative stress resulting in apoptosis and destruction of tissues. Although several treatments are available to treat the symptoms, the underlying causes are not well addressed. One of the most promising approaches to tackle the ill effects and the primary causes of DM is mesenchymal stem cell (MSC) therapy. The use of MSC therapy, because of the immunomodulatory and regenerative properties recorded in this type of cells in a number of experiments carried out in animal models and clinical trials of the disease, has reported positive outcomes. This review covers the principal mechanisms of action induced during MSC therapy in reference to the described pathophysiological pathways of both T1D and T2D. In addition, how this therapeutic intervention can counteract the ill effects of this condition leading to the promotion of tissue regeneration has been covered.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"662-668"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9648478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Progress on Cardiac Tissue Construction of Mesenchymal Stem Cells for Myocardial Infarction. 间充质干细胞用于心肌梗死的心脏组织构建研究进展。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X18666230823091017

Guo-Dong Yang, Da-Shi Ma, Chun-Ye Ma, Yang Bai

引用次数: 0

FBLN5 was Regulated by PRDM9, and Promoted Senescence and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells. FBLN5受PRDM9调控，促进人牙周韧带干细胞的衰老和成骨分化

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X18666230822100054

Mengyao Zhao, Rong Rong, Chen Zhang, Haoqing Yang, Xiao Han, Zhipeng Fan, Ying Zheng, Jianpeng Zhang

{"title":"FBLN5 was Regulated by PRDM9, and Promoted Senescence and Osteogenic Differentiation of Human Periodontal Ligament Stem Cells.","authors":"Mengyao Zhao, Rong Rong, Chen Zhang, Haoqing Yang, Xiao Han, Zhipeng Fan, Ying Zheng, Jianpeng Zhang","doi":"10.2174/1574888X18666230822100054","DOIUrl":"10.2174/1574888X18666230822100054","url":null,"abstract":"Objectives: Periodontal ligament stem cells (PDLSCs) are ideal seed cells for periodontal tissue regeneration. Our previous studies have indicated that the histone methyltransferase PRDM9 plays an important role in human periodontal ligament stem cells (hPDLSCs). Whether FBLN5, which is a downstream gene of PRDM9, also has a potential impact on hPDLSCs is still unclear.Methods: Senescence was assessed using β-galactosidase and Enzyme-linked immunosorbent assay (ELISA). Osteogenic differentiation potential of hPDLSCs was measured through Alkaline phosphatase (ALP) activity assay and Alizarin red detection, while gene expression levels were evaluated using western blot and RT-qPCR analysis.Results: FBLN5 overexpression promoted the osteogenic differentiation and senescence of hPDLSCs. FBLN5 knockdown inhibited the osteogenic differentiation and senescence of hPDLSCs. Knockdown of PRDM9 decreased the expression of FBLN5 in hPDLSCs and inhibited senescence of hPDLSCs. Additionally, both FBLN5 and PRDM9 promoted the expression of phosphorylated p38 MAPK, Erk1/2 and JNK. The p38 MAPK pathway inhibitor SB203580 and the Erk1/2 pathway inhibitor PD98059 have the same effects on inhibiting the osteogenic differentiation and senescence of hPDLSCs. The JNK pathway inhibitor SP600125 reduced the senescence of hPDLSCs.Conclusion: FBLN5 promoted senescence and osteogenic differentiation of hPDLSCs via activation of the MAPK signaling pathway. FBLN5 was positively targeted by PRDM9, which also activated the MAPK signaling pathway.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"417-425"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10788921/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10407784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exosome-mediated Repair of Intervertebral Disc Degeneration: The Potential Role of miRNAs. 外泌体介导的椎间盘退化修复：miRNAs 的潜在作用

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X18666230504094233

Han-Shi Wang, Shu Lin, Hai-Ming Yu

{"title":"Exosome-mediated Repair of Intervertebral Disc Degeneration: The Potential Role of miRNAs.","authors":"Han-Shi Wang, Shu Lin, Hai-Ming Yu","doi":"10.2174/1574888X18666230504094233","DOIUrl":"10.2174/1574888X18666230504094233","url":null,"abstract":"Intervertebral disc degeneration (IVDD) is a serious condition that manifests as low back pain, intervertebral disc protrusion, and spinal canal stenosis. At present, the main treatment methods for IVDD are surgical interventions such as discectomy, total disc replacement, and spinal fusion. However, these interventions have shown limitations, such as recurrent lumbar disc herniation after discectomy, lesions in adjacent segments, and failure of fixation. To overcome these shortcomings, researchers have been exploring stem cell transplantation therapy, such as mesenchymal stem cell (MSC) transplantation, but the treatment results are still controversial. Therefore, researchers are in search of new methods that are more efficient and have better outcomes. The exosomes from stem cells contain a variety of bioactive molecules that mediate cell interactions, and these components have been investigated for their potential therapeutic role in the repair of various tissue injuries. Recent studies have shown that MSC-derived miRNAs in exosomes and vesicles have therapeutic effects on nucleus pulposus cells, annulus fibrosus, and cartilage endplate. miRNAs play a role in many cell activities, such as cell proliferation, apoptosis, and cytokine release, by acting on mRNA translation, and they may have immense therapeutic potential, especially when combined with stem cell therapy. This article reviews the current status of research on intervertebral disc repair, especially with regard to the latest research findings on the molecular biological mechanisms of miRNAs in MSC-derived exosomes in intervertebral disc repair.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"798-808"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9425084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conditioned Media Therapy in Alzheimer's Disease: Current Findings and Future Challenges. 阿尔茨海默病的条件介质疗法：当前发现与未来挑战

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X18666230523155659

Amin Firoozi, Mehri Shadi, Zohre Aghaei, Mohammad Reza Namavar

{"title":"Conditioned Media Therapy in Alzheimer's Disease: Current Findings and Future Challenges.","authors":"Amin Firoozi, Mehri Shadi, Zohre Aghaei, Mohammad Reza Namavar","doi":"10.2174/1574888X18666230523155659","DOIUrl":"10.2174/1574888X18666230523155659","url":null,"abstract":"Alzheimer's disease (AD) is a neurodegenerative disorder accompanied by a reduction in cognition and memory. Till now, there is no definite cure for AD, although, there are treatments available that may improve some symptoms. Currently, in regenerative medicine stem cells are widely used, mainly for treating neurodegenerative diseases. There are numerous forms of stem cells to treat AD aiming at the expansion of the treatment methods for this particular disease. Since 10 years ago, science has gained abundant knowledge to treat AD by understanding the sorts of stem cells, methods, and phasing of injection. Besides, due to the side effects of stem cell therapy like the potentiation for cancer, and as it is hard to follow the cells through the matrix of the brain, researchers have presented a new therapy for AD. They prefer to use conditioned media (CM) that are full of different growth factors, cytokines, chemokines, enzymes, etc. without tumorigenicity or immunogenicity such as stem cells. Another benefit of CM is that CM could be kept in the freezer, easily packaged, and transported, and doesn't need to fit with the donor. Due to the beneficial effects of CM, in this paper, we intend to evaluate the effects of various types of CM of stem cells on AD.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"700-711"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9570489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Potential of Stem Cells in Treating Breast Cancer. 干细胞治疗乳腺癌的潜力。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X18666230428094056

Deepika Yadav, Pramod Kumar Sharma, Prem Shankar Mishra, Rishabha Malviya

引用次数: 0

Bone Marrow Mesenchymal Stem Cell Extracellular Vesicle-derived miR-27b- 3p activates the Wnt/Β-catenin Pathway by Targeting SMAD4 and Aggravates Hepatic Ischemia-reperfusion Injury. 骨髓间充质干细胞细胞外载体衍生的 miR-27b- 3p 通过靶向 SMAD4 激活 Wnt/Β-catenin 通路并加重肝缺血再灌注损伤

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X19666230901140628

Hongnan Li, Weidong Lin, Yunlei Li, Jiayang Zhang, Runsheng Liu, Minghai Qu, Ruihua Wang, Xiaomin Kang, Xuekun Xing

{"title":"Bone Marrow Mesenchymal Stem Cell Extracellular Vesicle-derived miR-27b- 3p activates the Wnt/Β-catenin Pathway by Targeting SMAD4 and Aggravates Hepatic Ischemia-reperfusion Injury.","authors":"Hongnan Li, Weidong Lin, Yunlei Li, Jiayang Zhang, Runsheng Liu, Minghai Qu, Ruihua Wang, Xiaomin Kang, Xuekun Xing","doi":"10.2174/1574888X19666230901140628","DOIUrl":"10.2174/1574888X19666230901140628","url":null,"abstract":"Background: To investigate the roles of extracellular vesicles (EVs) secreted from bone marrow mesenchymal stem cells (BMSCs) and miR-27 (highly expressed in BMSC EVs) in hepatic ischemia‒ reperfusion injury (HIRI).Approaches and results: We constructed a HIRI mouse model and pretreated it with an injection of agomir-miR-27-3p, agomir-NC, BMSC-EVs or control normal PBS into the abdominal cavity. Compared with the HIRI group, HIRI mice preinjected with BMSC-EVs had significantly decreased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels and alleviated liver necrosis (P<0.05). However, compared with HIRI+NC mice, HIRI+miR-27b mice had significantly increased ALT and AST levels, aggravated liver necrosis, and increased apoptosis-related protein expression (P<0.05). The proliferation and apoptosis of AML-12 cells transfected with miR-27 were significantly higher than the proliferation and apoptosis of AML-12 cells in the mimic NC group (P<0.01) after hypoxia induction. SMAD4 was proven to be a miR-27 target gene. Furthermore, compared to HIRI+NC mice, HIRI+miR-27 mice displayed extremely reduced SMAD4 expression and increased levels of wnt1, β-catenin, c-Myc, and Cyclin D1.Conclusion: Our findings reveal the role and mechanism of miR-27 in HIRI and provide novel insights for the prevention and treatment of HIRI; for example, EVs derived from BMSCs transfected with antimiR- 27 might demonstrate better protection against HIRI.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"755-766"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10553356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Mesenchymal Stem Cells in Experimental Ovarian Damage. 在实验性卵巢损伤中使用间充质干细胞。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X18666230713121530

Hanan Fouad, Ibrahim A Albahlol, Hazim A Wahab, Eman H Nadwa, Heba M Galal, Mohamed Abouelkheir, Ahmed E Taha, Abdelkarim G Kamel, Hassan A Abdelmawlla

{"title":"Use of Mesenchymal Stem Cells in Experimental Ovarian Damage.","authors":"Hanan Fouad, Ibrahim A Albahlol, Hazim A Wahab, Eman H Nadwa, Heba M Galal, Mohamed Abouelkheir, Ahmed E Taha, Abdelkarim G Kamel, Hassan A Abdelmawlla","doi":"10.2174/1574888X18666230713121530","DOIUrl":"10.2174/1574888X18666230713121530","url":null,"abstract":"Background: Bisphenol-A (BPA) has a well-proven deleterious effect on the hypothalamicpituitary- gonadal axis.Objectives: The current study investigated the therapeutic potentials of mesenchymal stem cells (MSCs) in a murine model of BPA-induced ovarian damage.Methods: Fifty adult female rats were divided into: Group 1; control group, Group IIa, IIb: rats were given oral gavage of BPA (25 and 50 mg/Kg body weight respectively) on a daily basis for 15 days, and Group IIIa, IIIb; rats were intravenously treated with of MSCs (106 cells) after receiving the last dose of BPA as in group II. Plasma and ovarian tissue levels of Malondialdehyde (MDA) and gonadal axis hormones were assessed. Apoptosis was evaluated by TUNNEL assay and by apoptosis markers (FAS, FASL, Caspase 3, SLTM). A histological examination of ovarian tissue was also conducted.Results: BPA resulted in a significant elevation in plasma levels of LH, FSH, and ovarian tissue levels of MDA and a significant decrease in estradiol and progesterone. All genetic and protein markers of apoptosis were elevated in BPA treated group with decreased oestrogen receptor expression in the ovarian tissue. Increased apoptotic cells were confirmed by TUNEL assay. A high dose of BPA was able to increase the number of atretic follicles in the ovarian tissue whereas the numbers of primordial, primary, secondary and Graafian follicles were decreased. All the laboratory and histological abnormalities were ameliorated by treatment with MSCs.Conclusion: The antioxidant and anti-apoptotic effects of MSCs could possibly explain the ability of this therapeutic modality to ameliorate BPA-induced-ovarian damage.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"725-734"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9832285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation of Pancreatic Beta Cells: Dual Acting of Inflammatory Factors. 胰腺 Beta 细胞的分化：炎症因子的双重作用。

IF 2.7 4区医学

Current stem cell research & therapy Pub Date : 2024-01-01 DOI: 10.2174/1574888X18666230504093649

Faeze Shahedi, Arron Munggela Foma, Azam Mahmoudi-Aznaveh, Mohammad Ali Mazlomi, Zahra Azizi, Mohammad Reza Khorramizadeh

{"title":"Differentiation of Pancreatic Beta Cells: Dual Acting of Inflammatory Factors.","authors":"Faeze Shahedi, Arron Munggela Foma, Azam Mahmoudi-Aznaveh, Mohammad Ali Mazlomi, Zahra Azizi, Mohammad Reza Khorramizadeh","doi":"10.2174/1574888X18666230504093649","DOIUrl":"10.2174/1574888X18666230504093649","url":null,"abstract":"In the past decades, scientists have made outstanding efforts to treat diabetes. However, diabetes treatment is still far from satisfactory due to the complex nature of the disease and the challenges encountered in resolving it. Inflammatory factors are key regulators of the immune system's response to pathological insults, organ neogenesis, rejuvenation of novel cells to replace injured cells and overwhelming disease conditions. Currently, the available treatments for type 1 diabetes include daily insulin injection, pancreatic beta cell or tissue transplantation, and gene therapy. Cell therapy, exploiting differentiation, and reprogramming various types of cells to generate pancreatic insulin-producing cells are novel approaches for the treatment of type 1 diabetes. A better understanding of the inflammatory pathways offers valuable and improved therapeutic options to provide more advanced and better treatments for diabetes. In this review, we investigated different types of inflammatory factors that participate in the pathogenesis of type 1 diabetes, their possible dual impacts on the differentiation, reprogramming, and fusion of other stem cell lines into pancreatic insulin-producing beta cells, and the possibility of applying these factors to improve the treatment of this disease.","PeriodicalId":10979,"journal":{"name":"Current stem cell research & therapy","volume":" ","pages":"832-839"},"PeriodicalIF":2.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9418175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0