{"title":"LGALS3 promotes the proliferation of Liaoning Cashmere goat skin fibroblasts by regulating the expression of PIEZO1","authors":"Mei Jin, Weiyu Fan, Jinxu Xu","doi":"10.17221/72/2024-cjas","DOIUrl":"https://doi.org/10.17221/72/2024-cjas","url":null,"abstract":"<br />Long non-coding RNA (lncRNA) plays a role in the reconstruction of hair follicles and in the formation and growth of cashmere fibres. One cashmere growth-associated lncRNA was successfully screened out at the early stage in the laboratory, which was named LncRNA MTC. In this study, the binding of galectin 3 (LGALS3) to LncRNA MTC was examined through RNA immunoprecipitation (RIP) in skin fibroblasts of Liaoning Cashmere goats. The pLenti-LGALS3-His-IRES-EGFP lentiviral overexpression vector was built, and the result of CCK8 indicated that the overexpression of LGALS3 significantly enhanced the viability of skin fibroblasts (<i>P</i><i> </i>< 0.05). A total of 15 proteins interacting with LGALS3 were identified through co-immunoprecipitation (CO-IP) combined mass spectrometry, which were primarily rich in ECM-receptor interaction, focal adhesion, PI3K-AKT, and other signalling pathways. Moreover, PIEZO1 refers to a mechanically sensitive ion channel protein that is capable of regulating the cell proliferation. As revealed by qPCR results, overexpression of <i>LGALS3</i> significantly upregulated the expression of <i>PIEZO1</i>, which was effectively interfered by siRNA. The result of CCK8 indicated that siPIEZO1 significantly inhibited cell proliferation, whereas overexpression of LGALS3 protected cells from the suppression of siPIEZO1 (<i>P</i><i> </i>< 0.01). This study revealed that LGALS3 is capable of stimulating the proliferation of skin fibroblasts by regulating<i> PIEZO1</i>. The effect of LGALS3 on the proliferation of skin fibroblasts from the protein interaction was explored, and this study is expected to lay a certain scientific basis for the research on the hair follicle development mechanism of Liaoning Cashmere goats.","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":"70 3","pages":"102-112"},"PeriodicalIF":0.0,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://cjas.agriculturejournals.cz/doi/10.17221/72/2024-CJAS.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147887647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Savvulidi, M. Ptáček, Szabolcs Nagy, L. Stádník
{"title":"Meta-analysis of fluorochrome concentrations to assess bull sperm quality by flow cytometry in 2021-2023","authors":"F. Savvulidi, M. Ptáček, Szabolcs Nagy, L. Stádník","doi":"10.17221/152/2023-cjas","DOIUrl":"https://doi.org/10.17221/152/2023-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":"1 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139168961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of catalase on the growth performance, antioxidation, and microbial metabolism of weaned rabbits","authors":"Ning Liu, Yien Lin, Shuaibao Wang, Jianping Wang","doi":"10.17221/133/2023-cjas","DOIUrl":"https://doi.org/10.17221/133/2023-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":"47 11","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138954627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Momčilo Šaran, Ljuba Štrbac, D. Janković, M. Djan, S. Trivunović, M. Zorc
{"title":"Genomic characterization of Serbian Holstein-Friesian cattle population","authors":"Momčilo Šaran, Ljuba Štrbac, D. Janković, M. Djan, S. Trivunović, M. Zorc","doi":"10.17221/89/2023-cjas","DOIUrl":"https://doi.org/10.17221/89/2023-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":"32 5","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138996488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The occurrence and risk assessment of bisphenol A and its analogues - bisphenol B, F, S, Z and AF in the urine of lactating sows","authors":"I. Bahelka, R. Stupka, J. Čítek, M. Šprysl","doi":"10.17221/90/2023-cjas","DOIUrl":"https://doi.org/10.17221/90/2023-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":"3 2","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138625374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Kašná, L. Zavadilová, Z. Krupová, S. Šlosárková, P. Fleischer
{"title":"The most common reproductive disorders of cows in Holstein cattle breeding","authors":"E. Kašná, L. Zavadilová, Z. Krupová, S. Šlosárková, P. Fleischer","doi":"10.17221/86/2023-cjas","DOIUrl":"https://doi.org/10.17221/86/2023-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138612608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Lipid-rich bovine serum albumin is beneficial for the early development of bovine embryos, but induces lipid droplet formation in the blastocyst","authors":"Dae-Jin Kwon, Kyung-Do Park, Lee HakKyo, J. Oh","doi":"10.17221/107/2023-cjas","DOIUrl":"https://doi.org/10.17221/107/2023-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":" 83","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138612049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Ptáček, M. Milerski, Tereza Michlová, J. Ducháček, V. Tančin, M. Uhrinčať, J. Schmidová, F. Savvulidi, L. Stádník
{"title":"Monitoring of milk performance of Wallachian sheep grazed under traditional Carpathian management in Western Beskids location","authors":"M. Ptáček, M. Milerski, Tereza Michlová, J. Ducháček, V. Tančin, M. Uhrinčať, J. Schmidová, F. Savvulidi, L. Stádník","doi":"10.17221/85/2023-cjas","DOIUrl":"https://doi.org/10.17221/85/2023-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":"54 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tomáš Janoš, Jan Kuchtík, Eliška Dračková, Martin Hošek, Tomáš Kopec, Radek Filipčík
{"title":"Growth, carcass and meat quality in Zwartbles lambs slaughtered at different live weights","authors":"Tomáš Janoš, Jan Kuchtík, Eliška Dračková, Martin Hošek, Tomáš Kopec, Radek Filipčík","doi":"10.17221/99/2023-cjas","DOIUrl":"https://doi.org/10.17221/99/2023-cjas","url":null,"abstract":"","PeriodicalId":10972,"journal":{"name":"Czech Journal of Animal Science","volume":"32 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135218195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}