Cytometry最新文献_第9页

High-throughput pretreatment system in automated urinary sediment analyzer. 全自动尿沉渣分析仪的高通量预处理系统。

Cytometry Pub Date : 2000-01-01

R Miyake, I Yamazaki, Y Kojima, M Kurimura, H Horiuchi

引用次数: 0

Robust autofocusing in microscopy. 强大的自动聚焦显微镜。

Cytometry Pub Date : 2000-01-01

J M Geusebroek, F Cornelissen, A W Smeulders, H Geerts

引用次数: 0

Increased peripheral blood gamma delta T-cells in patients with lymphoid neoplasia: A diagnostic dilemma in flow cytometry. 淋巴样肿瘤患者外周血γ δ t细胞增加:流式细胞术的诊断困境。

Cytometry Pub Date : 1999-12-15

J McClanahan, P I Fukushima, M Stetler-Stevenson

引用次数: 0

Clinical cytometry society 14(th) annual meeting clinical applications of cytometry, september 26-29, 1999, Marriott's rancho las palmas resort, palm springs, CA: meeting program 临床细胞术学会第14届年度会议:细胞术临床应用，1999年9月26-29日，加州棕榈泉万豪酒店

Cytometry Pub Date : 1999-12-15

引用次数: 0

Forum: journal club 论坛:杂志社

Cytometry Pub Date : 1999-12-15

引用次数: 0

Single- versus dual-platform assays for human CD34+ cell enumeration. 人CD34+细胞计数的单平台与双平台测定。

Cytometry Pub Date : 1999-12-15

I L Barbosa, M E Sousa, M I Godinho, F Sousa, A Carvalhais

{"title":"Single- versus dual-platform assays for human CD34+ cell enumeration.","authors":"I L Barbosa, M E Sousa, M I Godinho, F Sousa, A Carvalhais","doi":"","DOIUrl":"","url":null,"abstract":"We comparatively assessed CD34+ cell quantification by two of the recently available single platform assays, the IMAGN 2000 STELLer (Immucor, Lisbon, Portugal) microvolume fluorimetry and the ProCOUNT (BD-ENZIfarma, Lisbon, Portugal) flow cytometry, with our \"in-house\" dual-platform flow cytometric assay. The performance of the methods was evaluated by linearity and reproducibility tests. The linearity study, over a range of 0-1,200 CD34+ cell/microl, gave a good linear relationship for the three methods, with R(2) > 0.99. Precision tested at three different concentrations gave coefficients of variation ranging from 3.6-26.4% for the STELLertrade mark, 2.4-13.8% for the ProCOUNT, and 3.2-6.4% for flow cytometry. CD34+ cells were quantified in umbilical cord blood (UCB), UCB enriched-leukocyte buffy-coat (BC), mobilized peripheral blood (PB) and mobilized peripheral blood progenitor cells (PBPC) collected by leucapheresis, from a total of 72 samples. Flow cytometric results showed good linear correlation to the absolute counts obtained by the STELLer and ProCOUNT for all samples (R > 0.90 for all methods), with no differences when compared by paired tests (P > 0.05). Linear correlations between methods were also found when individually looking at the different cell sources: UCB or PB, BC, and PBPC, with low, intermediate and high CD34+ cell concentrations, respectively. Furthermore, with the exception of a significant difference between the ProCOUNT and STELLer results for UCB (P < 0.05), no other difference between methods was found for each of the individual populations (P > 0.05). To our knowledge, this is the first report in which the results are presented and analyzed according to each source of CD34+ cells. Our results show that the STELLer and the ProCOUNT are equally efficient for the dual-platform flow cytometric assay in CD34+ cell quantification.","PeriodicalId":10947,"journal":{"name":"Cytometry","volume":"38 6","pages":"274-9"},"PeriodicalIF":0.0,"publicationDate":"1999-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21446931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Photothermal studies of modulating effect of photoactivated chlorin on interaction of blood cells with bacteria. 光活化氯对血细胞与细菌相互作用调节作用的光热研究。

Cytometry Pub Date : 1999-12-01

D Lapotko, T Romanovskaya, G Kutchinsky, V Zharov

引用次数: 0

Image analysis software for automatic DNA ploidy assessment of archival solid tumours. 档案实体瘤DNA倍性自动评估的图像分析软件。

Cytometry Pub Date : 1999-12-01

D Bloyet, P Herlin, E Masson, B Plancoulaine, F Duigou, F Angot, J P Signolle, D Deman, A M Mandard, P Belhomme, T Datry, O Rougereau

{"title":"Image analysis software for automatic DNA ploidy assessment of archival solid tumours.","authors":"D Bloyet, P Herlin, E Masson, B Plancoulaine, F Duigou, F Angot, J P Signolle, D Deman, A M Mandard, P Belhomme, T Datry, O Rougereau","doi":"","DOIUrl":"","url":null,"abstract":"Background: Image cytometry has proved to provide a good alternative to flow cytometry for DNA ploidy measurement of archival tumors. However, when interactively done this technique is unable to give statistically valuable results within an acceptable time for clinical oncology.Methods: An image cytometer was developed for fully automatic DNA ploidy quantitation, focusing efforts on speed and accuracy. Software functionalities include systematic acquisition of fields on a microscopic slide, detection, localization and sorting of nuclei, computation of the DNA content together with post-processing tools, for a deeper analysis of the DNA ploidy diagram.Results: DNA ploidy analysis of archival breast carcinoma samples illustrates the accuracy of DNA ploidy measurements and the sensitivity in the detection of DNA ploidy abnormalities as a result of cell sorting.Conclusions: Fully automatic image cytometry is able to combine qualities of flow cytometry (automatic analysis of a statistically significant collection of cell nuclei) with additional advantages: sorting of unwanted events (debris, stromal and inflammatory cell nuclei) and facilities for an a posteriori control of the quality of cell selection. This method is well suited to DNA ploidy analysis of archival cancer samples.","PeriodicalId":10947,"journal":{"name":"Cytometry","volume":"37 4","pages":"267-74"},"PeriodicalIF":0.0,"publicationDate":"1999-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21409158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safe sorting of GFP-transduced live cells for subsequent culture using a modified FACS vantage. 使用改进的FACS优势对gfp转导的活细胞进行后续培养的安全分选。

Cytometry Pub Date : 1999-12-01

T U Sørensen, G J Gram, S D Nielsen, J E Hansen

{"title":"Safe sorting of GFP-transduced live cells for subsequent culture using a modified FACS vantage.","authors":"T U Sørensen, G J Gram, S D Nielsen, J E Hansen","doi":"","DOIUrl":"","url":null,"abstract":"Background: A stream-in-air cell sorter enables rapid sorting to a high purity, but it is not well suited for sorting of infectious material due to the risk of airborne spread to the surroundings.Methods: A FACS Vantage cell sorter was modified for safe use with potentially HIV infected cells. Safety tests with bacteriophages were performed to evaluate the potential spread of biologically active material during cell sorting. Cells transduced with a retroviral vector carrying the gene for GFP were sorted on the basis of their GFP fluorescence, and GFP expression was followed during subsequent culture.Results: The bacteriophage sorting showed that the biologically active material was confined to the sorting chamber. A failure mode simulating a nozzle blockage resulted in detectable droplets inside the sorting chamber, but no droplets could be detected when an additional air suction from the sorting chamber had been put on. The GFP transduced cells were sorted to 99% purity. Cells not expressing GFP at the time of sorting did not turn on the gene during subsequent culture. Un-sorted cells and cells sorted to be positive for GFP showed a decrease in the fraction of GFP positive cells during culture.Conclusions: Sorting of live infected cells can be performed safely and with no deleterious effects on vector expression using the modified FACS Vantage instrument.","PeriodicalId":10947,"journal":{"name":"Cytometry","volume":"37 4","pages":"284-90"},"PeriodicalIF":0.0,"publicationDate":"1999-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21409160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of endogenous alkaline phosphatase activity in intact cells by flow cytometry using the fluorogenic ELF-97 phosphatase substrate. 用荧光性ELF-97磷酸酶底物流式细胞术检测完整细胞内源性碱性磷酸酶活性。

Cytometry Pub Date : 1999-12-01

W G Telford, W G Cox, D Stiner, V L Singer, S B Doty

{"title":"Detection of endogenous alkaline phosphatase activity in intact cells by flow cytometry using the fluorogenic ELF-97 phosphatase substrate.","authors":"W G Telford, W G Cox, D Stiner, V L Singer, S B Doty","doi":"","DOIUrl":"","url":null,"abstract":"Background: The alkaline phosphatase (AP) substrate 2-(5'-chloro-2'-phosphoryloxyphenyl)-6-chloro-4-(3H)-quinazolinone (ELF((R))-97 for enzyme-labeled fluorescence) has been found useful for the histochemical detection of endogenous AP activity and AP-tagged proteins and oligonucleotide probes. In this study, we evaluated its effectiveness at detecting endogenous AP activity by flow cytometry.Methods: The ELF-97 phosphatase substrate was used to detect endogenous AP activity in UMR-106 rat osteosarcoma cells and primary cultures of chick chondrocytes. Cells were labeled with the ELF-97 reagent and analyzed by flow cytometry using an argon ultraviolet (UV) laser. For comparison purposes, cells were also assayed for AP using a Fast Red Violet LB azo dye assay previously described for use in detecting AP activity by flow cytometry.Results: The ELF-97 phosphatase substrate effectively detected endogenous AP activity in UMR-106 cells, with over 95% of the resulting fluorescent signal resulting from AP-specific activity (as determined by levamisole inhibition of AP activity). In contrast, less than 70% of the fluorescent signal from the Fast Red Violet LB (FRV) assay was AP-dependent, reflecting the high intrinsic fluorescence of the unreacted components. The ELF-97 phosphatase assay was also able to detect very low AP activity in chick chondrocytes that was undetectable by the azo dye method.Conclusions: The ELF-97 phosphatase assay was able to detect endogenous AP activity in fixed mammalian and avian cells by flow cytometry with superior sensitivity to previously described assays. This work also shows the applicability of ELF-97 to flow cytometry, supplementing its previously demonstrated histochemical applications.","PeriodicalId":10947,"journal":{"name":"Cytometry","volume":"37 4","pages":"314-9"},"PeriodicalIF":0.0,"publicationDate":"1999-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21409164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0