R Miyake, I Yamazaki, Y Kojima, M Kurimura, H Horiuchi
{"title":"High-throughput pretreatment system in automated urinary sediment analyzer.","authors":"R Miyake, I Yamazaki, Y Kojima, M Kurimura, H Horiuchi","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Urine contains microscopically observable particles that can indicate certain types of disease in the urinary tract system. Determining these various types of sediments by manual operation is a cumbersome and time-consuming task. To eliminate this labor, we developed an automated urinary sediment analyzer with high-throughput pretreatment system.</p><p><strong>Methods: </strong>The pretreatment system mainly consists of four reaction vessels for dying samples (urine), a sheath flow chamber, and an unique sample carrier mechanism from the reaction vessel to the flow chamber, which enables overlapped processing, and rapid transfer of samples with small dispersion and a short buildup time.</p><p><strong>Results: </strong>The buildup time was experimentally found to be 1.8 s, and the extra-sample volume beside that for measurement was only 4.9 microl (1/20 of the total sample volume).</p><p><strong>Conclusions: </strong>Short buildup time results in high throughput of 120 samples per hour, and relatively small extra-volume contributes to reduce carryover.</p>","PeriodicalId":10947,"journal":{"name":"Cytometry","volume":"39 1","pages":"67-71"},"PeriodicalIF":0.0000,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cytometry","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Background: Urine contains microscopically observable particles that can indicate certain types of disease in the urinary tract system. Determining these various types of sediments by manual operation is a cumbersome and time-consuming task. To eliminate this labor, we developed an automated urinary sediment analyzer with high-throughput pretreatment system.
Methods: The pretreatment system mainly consists of four reaction vessels for dying samples (urine), a sheath flow chamber, and an unique sample carrier mechanism from the reaction vessel to the flow chamber, which enables overlapped processing, and rapid transfer of samples with small dispersion and a short buildup time.
Results: The buildup time was experimentally found to be 1.8 s, and the extra-sample volume beside that for measurement was only 4.9 microl (1/20 of the total sample volume).
Conclusions: Short buildup time results in high throughput of 120 samples per hour, and relatively small extra-volume contributes to reduce carryover.
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