Current Research in Food Science最新文献

{"title":"Genome MLST scheme for tracing genetic diversity and multidrug resistance of food animal-derived Clostridium perfringens","authors":"Liting Wu ,&nbsp;Zugong Yu ,&nbsp;Penghui Li ,&nbsp;Zhenyu Wang ,&nbsp;Qiuchun Li ,&nbsp;Hongduo Bao ,&nbsp;Pilar García ,&nbsp;Andrey Letarov ,&nbsp;Yan Zhou ,&nbsp;Hui Zhang","doi":"10.1016/j.crfs.2025.101149","DOIUrl":"10.1016/j.crfs.2025.101149","url":null,"abstract":"<div><div><em>Clostridium perfringens</em> is an important food-borne anaerobic bacterium that can cause chronic disease in humans and animals. The complex toxins that it produces can cause diarrhea, necrotizing enteritis, and even death. This study aimed to evaluate the presence of antibiotic resistance genes and determinants of toxin production in various strains of <em>C. perfringens</em>, derived from different geographical locations and animal sources, through whole-genome sequencing (WGS) and bioinformatics analysis. Antimicrobial resistance testing revealed a notable prevalence of resistance among isolates, with 39.5 % being resistant to clindamycin and 32.6 % resistant to tetracycline. Additionally, 29.1 % of the isolates were found to be resistant to at least two classes of commonly used antibiotics. T<em>etA</em>(<em>P</em>) and <em>tetB</em>(<em>P</em>), associated with tetracycline resistance, were the most frequently identified resistance genes, present in 93.0 % and 79.0 % of the isolates, respectively. Virulence genes encoding extracellular collagenases (<em>colA</em> and <em>colSI</em>), alpha-toxin (<em>plc</em>), and sialidase (<em>nanH</em>) were detected in 85 isolates, representing 99 % of all sequenced strains. Notably, the gene encoding <em>C. perfringens</em> enterotoxin (<em>cpe</em>) was identified in only one isolate, which originated from chicken. Multi-locus sequence typing revealed that the 86 representative isolates belonged to 49 sequence types (STs), including 33 unique, previously uncharacterized STs. Furthermore, 30.23 % of these STs were grouped into six clonal complexes (CCs). CgSNP analysis of globally circulating isolates demonstrated that the prevalence of the virulence gene <em>cpe</em> was higher in these isolates than in clinical strains. The identification of multi-drug resistance and toxin-encoding genes among the isolates underscores the concerning spread of antimicrobial resistance among <em>C. perfringens</em> affecting both animals and humans. The diversity of CRISPR/Cas system provided addition insight into complex genetic correlations and evolutionary dynamics of pathogenic <em>C. perfringens</em>. Notably, the phage SD72 exhibited a broad inhibitory effect against <em>C. perfringens</em> isolates, irrespective of STs types and antimicrobial resistance (74/86, 80.1 %). These findings highlight the urgent need for enhanced epidemic surveillance of <em>C. perfringens</em> in livestock settings to mitigate the risks of human transmission via environmental or food sources. Additionally, bacteriophages present a promising avenue as bio-antimicrobial agents for controlling the transmission of <em>C. perfringens</em> from animal sources to humans.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"11 ","pages":"Article 101149"},"PeriodicalIF":6.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144687500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combining rapid evaporative ionization mass spectrometry and chemometrics for the differentiation of cocoa bean quality","authors":"Julie Lestang ,&nbsp;Susanne Miescher Schwenninger ,&nbsp;Laura Nyström","doi":"10.1016/j.crfs.2025.101161","DOIUrl":"10.1016/j.crfs.2025.101161","url":null,"abstract":"<div><div>Ensuring the high and consistent quality of cocoa beans presents a significant challenge, driven by concerns related to food safety, economic profitability, and overall quality. Recently, functional microbial cultures have been developed by selecting specific microbial strains that enhance the cocoa bean fermentation process and improve the quality of the fermented and dried beans. These selection processes require extensive and time-consuming screening of numerous microbial strains. To address this, the present study explored a rapid, untargeted, metabolite-based approach to distinguish cocoa beans fermented with specific microbial cultures. This was achieved using rapid evaporative ionization mass spectrometry (REIMS) combined with chemometric analysis. Metabolite fingerprints of cocoa beans fermented with 21 antifungal (AF) and/or pectinolytic (P) microbial cultures were analyzed using REIMS. The fermented beans were differentiated based on their metabolite profiles using LiveID software, which is integrated with the REIMS system. Subsequently, six classification models were compared in detail to evaluate their performance, and tentatively extended to classify metabolite fingerprints from independently fermented beans. Initially, LiveID combined with PCA-LDA successfully distinguished metabolite fingerprints based on single-strain microbial cultures and the expected AF or AF&amp;P functionalities of co-cultures, achieving an accuracy of 80 %. Further analysis of the six classification models demonstrated the strong performance of gradient boosting machines, random forests, and neural networks in differentiating metabolite fingerprints based on the functionality of microbial co-cultures, with accuracy estimates of 85 %, 84 %, and 81 %, respectively. Finally, optimized random forest models were tested on an independent dataset, achieving 70–85 % accuracy for the two-class models. The performance of these models on independent data highlights their potential for broader applications, such as differentiating cocoa beans at the lab scale or in on-farm settings to support the development of functional microbial cultures for the production of cocoa beans with consistently high quality.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"11 ","pages":"Article 101161"},"PeriodicalIF":7.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144810595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vintage authentication technology for aged Baijiu: Acid-ester equilibrium","authors":"Shu Jiang ,&nbsp;Wei Dai ,&nbsp;Xuepeng Chen ,&nbsp;Wenjun Tan ,&nbsp;Jilei Wang ,&nbsp;Yougui Yu ,&nbsp;Qing Zheng","doi":"10.1016/j.crfs.2025.101168","DOIUrl":"10.1016/j.crfs.2025.101168","url":null,"abstract":"<div><div>This paper investigates a Baijiu vintage authentication technology based on the acid-ester equilibrium principle, providing a comprehensive analysis of its technical foundation, application outcomes, advantages. Research confirms that the aging process of Baijiu constitutes a dynamic acid-ester equilibrium system. As aging progresses, the liquor gradually approaches a state of thermodynamic equilibrium, with the equilibrium constant (K) stabilizing. By measuring the reaction quotient (Q) of ester compounds in the liquor and comparing it with the equilibrium constant (K), the aging status of Baijiu can be objectively and accurately evaluated, thereby enabling vintage authentication. Findings demonstrate the technology's strong universality across various aroma types and alcohol concentrations of Baijiu. Regardless of whether it is strong-aroma (<em>Nong</em>), sauce-aroma (<em>Jiang</em>), or other aroma types, or whether it is high-alcohol or low-alcohol Baijiu, the evolution patterns of acid-ester equilibrium remain consistent and effectively reflect the liquor's aging extent. The vintage prediction tool developed based on this technology has demonstrated outstanding performance in test cases, accurately categorizing Baijiu of different aging periods into corresponding vintage ranges, validating its practical application value.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"11 ","pages":"Article 101168"},"PeriodicalIF":7.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144830145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of L-arginine improving the macroscopic and microscopic qualities of Acipenser schrenckii surimi gel under low-salt conditions and microwave irradiation: Significance of moisture migration and distribution","authors":"Tong Shi ,&nbsp;Yu Xie ,&nbsp;Xiyue Cao ,&nbsp;Hao Zhang ,&nbsp;Chenya Lu ,&nbsp;Mengzhe Li ,&nbsp;Xin Wang ,&nbsp;Li Yuan ,&nbsp;Ruichang Gao","doi":"10.1016/j.crfs.2025.101018","DOIUrl":"10.1016/j.crfs.2025.101018","url":null,"abstract":"<div><div>This study explored the mechanism by which L-arginine (L-Arg) enhanced the characteristics of low-salt sturgeon (<em>Acipenser schrenckii</em>) surimi gels under microwave (MW) irradiation, focusing on the changes in the distribution and migration of water molecules. The findings indicated that L-Arg shortened the gel formation time from MW 3 min to MW 2 min. Moreover, the addition of 2% L-Arg optimized the gel properties, achieving the highest water holding capacity (83.24%), resilience (48.34 cm), and cohesion (0.80) at MW 3 min (<em>P</em> &lt; 0.05). Additionally, cryo-scanning electron microscopy showed that the fractal dimension and porosity of the gel network enhanced and decreased, respectively, with the increasing content of L-Arg. These improvements in macroscopic and microscopic qualities were related to the transformation of free water into immobilized water and the more uniform distribution of hydrogen protons under the MW irradiation promoted by L-Arg, as demonstrated by the magnetic resonance imaging and Pearson correlation analysis. This study provided novel insights into overcoming the challenges associated with MW processing in surimi products.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"10 ","pages":"Article 101018"},"PeriodicalIF":6.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143576890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant activity and mechanism of action of phycocyanin in bulk sunflower oil and respective oil-in-water emulsion","authors":"Elham Ehsandoost ,&nbsp;Mohammad Hadi Eskandari ,&nbsp;Malihe Keramat ,&nbsp;Mohammad-Taghi Golmakani","doi":"10.1016/j.crfs.2025.100981","DOIUrl":"10.1016/j.crfs.2025.100981","url":null,"abstract":"<div><div>This study compared the inhibitory mechanism of phycocyanin in sunflower oil with its activity in a sunflower oil-in-water emulsion. Additionally, the impact of lecithin on the inhibitory mechanism of phycocyanin in sunflower oil was evaluated. A sigmoidal model effectively described the oxidation kinetics. In both sunflower oil and sunflower oil-in-water emulsion, phycocyanin pro-oxidatively attacked lipid hydroperoxides besides inhibiting lipid hydroperoxides. The antioxidant activity of sunflower oil containing phycocyanin and lecithin was 2.2-fold greater than that of sunflower oil containing lecithin alone. The addition of lecithin enhanced the interfacial activity of phycocyanin and altered its hydrogen donating and electron transfer mechanisms. Also, by comparing the reverse micelles size samples of sunflower oil samples containing lecithin, we discovered that lecithin can enhance the potency of phycocyanin by boosting the ability of reverse micelles to incorporate lipid hydroperoxides within their structure.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"10 ","pages":"Article 100981"},"PeriodicalIF":6.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143096298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and characterization of a chicory extract fermented by Akkermansia muciniphila: An in vitro study on its potential to modulate obesity-related inflammation","authors":"A. Chervet ,&nbsp;R. Nehme ,&nbsp;C. Defois-Fraysse ,&nbsp;C. Decombat ,&nbsp;C. Blavignac ,&nbsp;C. Auxenfans ,&nbsp;B. Evrard ,&nbsp;S. Michel ,&nbsp;E. Filaire ,&nbsp;J.-Y. Berthon ,&nbsp;A. Dreux-Zigha ,&nbsp;L. Delort ,&nbsp;F. Caldefie-Chézet","doi":"10.1016/j.crfs.2025.100974","DOIUrl":"10.1016/j.crfs.2025.100974","url":null,"abstract":"<div><div>Obesity, the fifth leading cause of death globally and linked to chronic low-grade inflammation and development of numerous severe pathologies, is a major public health problem. Fermented foods, probiotics, and postbiotics emerge as promising avenues for combating obesity and inflammation. The aim of our study was to develop and characterize phyto-postbiotics corresponding to prebiotic compounds fermented by gut bacteria, which could act on obesity and related-inflammation. Chicory extract fermented by <em>Akkermansia muciniphila</em> (C-Akm) was selected as the most antioxidant of 20 fermented extracts. The identification of metabolites derived from C-Akm extract has enabled us to detect mostly amino acids, acids, and some polyphenols (daidzein and genistein). The anti-inflammatory and anti-obesity activities of C-Akm extract were studied by testing the extract (50 μg/mL) on the polarization of THP-1 into macrophages, the secretion of pro-inflammatory cytokines in LPS-stimulated PBMCs, and the secretion of leptin and adiponectin in adipospheroids derived from human adipose stem cells. Finally, the extract was examined in 3D co-culture model mimicking inflamed obese adipose tissue. We found that C-Akm extract decreased ROS generation, <em>TNF-α</em> and <em>Il-6</em> gene expression in polarized macrophages, INFγ and IL-17A secretion in LPS-stimulated PBMCs stimulated with LPS. It also decreased <em>leptin</em> expression while increasing <em>adiponectin</em> and <em>HSL</em> expression levels in both adipocytes and co-cultures. In addition, C-Akm extract stimulated adiponectin secretion in the co-culture model. Finally, our <em>in vitro</em> investigations demonstrated the potential benefits of C-Akm extract in the prevention and treatment of obesity-related inflammation.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"10 ","pages":"Article 100974"},"PeriodicalIF":6.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143095860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studying the impact of zein microfibers on the physicochemical and microstructural properties of bi-gels based on ι-carrageenan hydrogels and beeswax oleogels","authors":"Mojtaba Rezaei ,&nbsp;Sara Naji-Tabasi ,&nbsp;Behrouz Ghorani ,&nbsp;Bahareh Emadzadeh","doi":"10.1016/j.crfs.2025.100985","DOIUrl":"10.1016/j.crfs.2025.100985","url":null,"abstract":"<div><div>This research presents a novel bi-gel system formed by combining zein microfiber -reinforced carrageenan hydrogels and beeswax oleogels. The main objective is investigating the impact of the interplay between zein microfibers, ι-carrageenan hydrogels, beeswax oleogels on the properties of bi-gels. The study focused on bi-gel formulations combining beeswax oleogel and carrageenan, both plain and with zein microfibers. Different ratios of oleogel to ɩ-carrageenan hydrogel and oleogel to reinforced ɩ-carrageenan hydrogel were established: 5:95, 10:90, 15:85. The designed bi-gels exhibited semi-solid gel properties in rheological analysis, with increased oleogel content enhancing firmness, storage modulus, and loss modulus (<em>G'</em> &lt; <em>G″,</em> p &lt; 0.05). The incorporation of oleogel in the bi-gel substantially increased its consistency from 131 (g.s) to 668 (g.s) in the bi-gel containing 0.5% zein microfiber, 10% oleogel, and 90% hydrogel. FTIR results suggested that the bi-gels were formed through physical interactions without covalent cross-linking. Microfibers had a positive effect on the textural characteristics of bi-gels. The hardness of bi-gels increased from 13.26 to 35.12 g to 31-93-64.14 g after addition of microfibers. The BGZ10 formulation, consisting of 10% oleogel and 90% zein-reinforced hydrogel, showed the highest consistency among samples, with measurements of 668.48 ± 3.53 (g.s) and a <em>G′</em> value of 291000 ± 91.27 (Pa) (P &lt; 0.05). Additionally, the BGZ10 formulation displayed the highest complex viscosity, measuring at 47300 ± 20.73 (P &lt; 0.05). The thermal stability of bigel considerably increased by cooperation fibers in hydrogel. The developed bi-gels demonstrate significant potential for substituting conventional solid fats and introducing distinctive visual characteristics in various food products.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"10 ","pages":"Article 100985"},"PeriodicalIF":6.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143095863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperspectral imaging for detection of macronutrients retained in glutinous rice under different drying conditions","authors":"Kabiru Ayobami Jimoh ,&nbsp;Norhashila Hashim ,&nbsp;Rosnah Shamsudin ,&nbsp;Hasfalina Che Man ,&nbsp;Mahirah Jahari ,&nbsp;Puteri Nurain Megat Ahmad Azman ,&nbsp;Daniel I. Onwude","doi":"10.1016/j.crfs.2024.100963","DOIUrl":"10.1016/j.crfs.2024.100963","url":null,"abstract":"<div><div>This study detected the macronutrients retained in glutinous rice (GR) under different drying conditions by innovatively applying visible-near infrared hyperspectral imaging coupled with different spectra preprocessing and effective wavelength selection techniques (EWs). Subsequently, predictive models were developed based on processed spectra for the detection of the macronutrients, which include protein content (PC), moisture content (MC), fat content (FC), and ash content (AC). The result shows the raw spectra-based model had a prediction accuracy (<span><math><mrow><msubsup><mi>R</mi><mi>p</mi><mn>2</mn></msubsup></mrow></math></span>) of 0.6493, 0.9521, 0.4594, and 0.9773 for PC, MC, FC, and AC, respectively. Applying Savitzky Golay first derivatives (SG1D) method increases the <span><math><mrow><msubsup><mi>R</mi><mi>p</mi><mn>2</mn></msubsup></mrow></math></span> value to 0.9972, 0.9970, 0.9857 and 0.9972 for PC, MC, FC, and AC, respectively. Using the variable iterative space shrinkage algorithm (VISSA) as EWs reduces the spectral bands by over 60%, and this increases the accuracy of the model (SG1D-VISSA-PLSR) to 100%. Therefore, the developed SGID-VISSA-PLSR can be used to build a smart and reliable spectral system for detecting the macronutrients in GR grains.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"10 ","pages":"Article 100963"},"PeriodicalIF":6.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11732696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143001818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pan-genome perspective on the evolutionary dynamics of polyphyly, virulence, and antibiotic resistance in Salmonella enterica serovar Mbandaka highlights emerging threats to public health and food safety posed by cloud gene families","authors":"Nai-peng Kan ,&nbsp;Zhiqiu Yin ,&nbsp;Yu-feng Qiu ,&nbsp;Enhui Zheng ,&nbsp;Jianhui Chen ,&nbsp;Jianzhong Huang ,&nbsp;Yuhui Du","doi":"10.1016/j.crfs.2024.100957","DOIUrl":"10.1016/j.crfs.2024.100957","url":null,"abstract":"<div><div><em>Salmonella enterica</em> serovar Mbandaka, a prevalent foodborne pathogen, poses a threat to public health but remains poorly understood. We have determined the phylogenomic tree, genetic diversity, virulence, and antimicrobial resistance (AMR) profiles on a large genomic scale to elucidate the evolutionary dynamics within the Mbandaka pan-genome. The polyphyletic nature of this serovar is characterized by two distinct phylogenetic groups and inter-serovar recombination boundaries, that potentially arising from recombination events at the H2-antigen loci. The open pan-genome exhibited a flexible gene repertoire, with numerous cloud gene families involved in virulence and AMR. Extensive gene gain and loss observed at the terminal nodes of the phylogenetic tree indicate that Mbandaka individuals have undergone frequent gene turnover. The resulting changes in virulence and AMR genes potentially pose emerging threats to public health. We explored serovar conversion due to recombination of H-antigen loci, inter-serovar divergences in gene gain and loss, prophage-mediated acquisition of virulence factors, and the role of incompatibility group plasmids in acquiring resistance determinants as key molecular mechanisms driving the pathogenicity and antibiotic resistance of Mbandaka. Our work contributes to a comprehensive understanding of the complex mechanisms of pathogenesis and the ongoing evolutionary arms race with current therapeutic approaches in serovar Mbandaka.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"10 ","pages":"Article 100957"},"PeriodicalIF":6.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin alleviated chilling injury of cold-stored passion fruit by modulating cell membrane structure via acting on antioxidant ability and membrane lipid metabolism","authors":"Yuzhao Lin,&nbsp;Hongbin Chen,&nbsp;Yazhen Chen,&nbsp;Bowen Tan,&nbsp;Xuanjing Jiang","doi":"10.1016/j.crfs.2024.100951","DOIUrl":"10.1016/j.crfs.2024.100951","url":null,"abstract":"<div><div>Fresh passion fruit is sensitive to chilling injury (CI) during storage at improper low temperature of 5 °C, which lowers the fruit quality and limits its shelf life. The present study aimed to determine the impacts of melatonin on CI development of passion fruit in relation to antioxidant ability and membrane lipid metabolism during refrigeration. In present study, passion fruit was treated with 0.50 mmol L⁻¹ melatonin and distilled water (control) for 20 min, hereafter stockpiled at 5 °C. The results indicated that, in storage, melatonin-treated passion fruit showed the lower CI index and cell membrane permeability, lower superoxide anion production rate and malondialdehyde level, greater activities of catalase, superoxide dismutase and ascorbate peroxidase, higher levels of ascorbic acid and glutathione, and higher 1, 1-diphenyl-2-picrylhydrazyl radical scavenging capacity than control passion fruit. Besides, lower membrane lipid-degrading enzyme activities, lower contents of phosphatidic acid and saturated fatty acids (SFAs), higher levels of phosphatidylcholine, phosphatidylinositol and unsaturated fatty acids (USFAs), and greater ratio of USFAs to SFAs and index of USFAs were revealed in melatonin-treated passions than control passions. Thus, these results indicated that melatonin retained cell membrane structure via boosting antioxidant capacity and restricting membrane lipid degradation, accordingly increased the chilling resistance and delayed the CI development in fresh passion fruit.</div></div>","PeriodicalId":10939,"journal":{"name":"Current Research in Food Science","volume":"10 ","pages":"Article 100951"},"PeriodicalIF":6.2,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11721212/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}