Current Genomics最新文献

{"title":"Circular RNA Involvement in Aging and Longevity.","authors":"Ruize Niu,&nbsp;Jia Liu","doi":"10.2174/1389202923666220927110258","DOIUrl":"https://doi.org/10.2174/1389202923666220927110258","url":null,"abstract":"<p><strong>Background: </strong>Circular RNAs (circRNAs) are transcribed by RNA polymerase II and are mostly generated by the back-splicing of exons in the protein-coding gene. Massive circRNAs are reported to be differentially expressed in different species, implicating their prospects as aging biomarkers or regulators in the aging progression.</p><p><strong>Methods: </strong>The possible role of circRNAs in aging and longevity was reviewed by the query of circRNAs from literature reports related to tissue, organ or cellular senescence, and individual longevity.</p><p><strong>Results: </strong>A number of circRNAs have been found to positively and negatively modulate aging and longevity through canonical aging pathways in the invertebrates <i>Caenorhabditis elegans</i> and <i>Drosophila</i>. Recent studies have also shown that circRNAs regulate age-related processes and pathologies such various mammalian tissues, as the brain, serum, heart, and muscle. Besides, three identified representative circRNAs (circSfl, circGRIA1, and circNF1-419) were elucidated to correlate with aging and longevity.</p><p><strong>Conclusion: </strong>This review outlined the current studies of circRNAs in aging and longevity, highlighting the role of circRNAs as a biomarker of aging and as a regulator of longevity.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e3/38/CG-23-318.PMC9878857.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9484051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Update on Non-invasive Approaches for Genetic Testing of the Preimplantation Embryo.","authors":"Georgia Kakourou,&nbsp;Thalia Mamas,&nbsp;Christina Vrettou,&nbsp;Joanne Traeger-Synodinos","doi":"10.2174/1389202923666220927111158","DOIUrl":"https://doi.org/10.2174/1389202923666220927111158","url":null,"abstract":"<p><p>Preimplantation Genetic Testing (PGT) aims to reduce the chance of an affected pregnancy or improve success in an assisted reproduction cycle. Since the first established pregnancies in 1990, methodological approaches have greatly evolved, combined with significant advances in the embryological laboratory. The application of preimplantation testing has expanded, while the accuracy and reliability of monogenic and chromosomal analysis have improved. The procedure traditionally employs an invasive approach to assess the nucleic acid content of embryos. All biopsy procedures require high technical skill, and costly equipment, and may impact both the accuracy of genetic testing and embryo viability. To overcome these limitations, many researchers have focused on the analysis of cell-free DNA (cfDNA) at the preimplantation stage, sampled either from the blastocoel or embryo culture media, to determine the genetic status of the embryo non-invasively. Studies have assessed the origin of cfDNA and its application in non-invasive testing for monogenic disease and chromosomal aneuploidies. Herein, we discuss the state-of-the-art for modern non-invasive embryonic genetic material assessment in the context of PGT. The results are difficult to integrate due to numerous methodological differences between the studies, while further work is required to assess the suitability of cfDNA analysis for clinical application.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ef/67/CG-23-337.PMC9878856.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9484054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heuristic Analysis of Genomic Sequence Processing Models for High Efficiency Prediction: A Statistical Perspective.","authors":"Aditi R Durge,&nbsp;Deepti D Shrimankar,&nbsp;Ankush D Sawarkar","doi":"10.2174/1389202923666220927105311","DOIUrl":"https://doi.org/10.2174/1389202923666220927105311","url":null,"abstract":"<p><p>Genome sequences indicate a wide variety of characteristics, which include species and sub-species type, genotype, diseases, growth indicators, yield quality, <i>etc</i>. To analyze and study the characteristics of the genome sequences across different species, various deep learning models have been proposed by researchers, such as Convolutional Neural Networks (CNNs), Deep Belief Networks (DBNs), Multilayer Perceptrons (MLPs), <i>etc</i>., which vary in terms of evaluation performance, area of application and species that are processed. Due to a wide differentiation between the algorithmic implementations, it becomes difficult for research programmers to select the best possible genome processing model for their application. In order to facilitate this selection, the paper reviews a wide variety of such models and compares their performance in terms of accuracy, area of application, computational complexity, processing delay, precision and recall. Thus, in the present review, various deep learning and machine learning models have been presented that possess different accuracies for different applications. For multiple genomic data, Repeated Incremental Pruning to Produce Error Reduction with Support Vector Machine (Ripper SVM) outputs 99.7% of accuracy, and for cancer genomic data, it exhibits 99.27% of accuracy using the CNN Bayesian method. Whereas for Covid genome analysis, Bidirectional Long Short-Term Memory with CNN (BiLSTM CNN) exhibits the highest accuracy of 99.95%. A similar analysis of precision and recall of different models has been reviewed. Finally, this paper concludes with some interesting observations related to the genomic processing models and recommends applications for their efficient use.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f3/6a/CG-23-299.PMC9878859.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9484055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Non-invasive Prenatal Testing in Pregnancies Following Assisted Reproduction.","authors":"Vandana Kamath, Mary Purna Chacko, Mohan S Kamath","doi":"10.2174/1389202923666220518095758","DOIUrl":"10.2174/1389202923666220518095758","url":null,"abstract":"<p><p>In the decade since non-invasive prenatal testing (NIPT) was first implemented as a prenatal screening tool, it has gained recognition for its sensitivity and specificity in the detection of common aneuploidies. This review mainly focuses on the emerging role of NIPT in pregnancies following assisted reproductive technology (ART) in the light of current evidence and recommendations. It also deals with the challenges, shortcomings and interpretational difficulties related to NIPT in ART pregnancies, with particular emphasis on twin and vanishing twin pregnancies, which are widely regarded as the Achilles' heel of most pre-natal screening platforms. Future directions for exploration towards improving the performance and extending the scope of NIPT are also addressed.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3c/85/CG-23-326.PMC9878858.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9487152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of PARPi Resistance-related Competing Endogenous RNA Network.","authors":"Lili Kong,&nbsp;Jiaqi Xu,&nbsp;Lijun Yu,&nbsp;Shuo Liu,&nbsp;Zongjian Liu,&nbsp;Juanjuan Xiang","doi":"10.2174/1389202923666220527114108","DOIUrl":"https://doi.org/10.2174/1389202923666220527114108","url":null,"abstract":"<p><p><b><i>Objective</i>:</b> Ovarian cancer is a kind of common gynecological malignancy in women. PARP inhibitors (PARPi) have been approved for ovarian cancer treatment. However, the primary and acquired resistance have limited the application of PARPi. The mechanisms remain to be elucidated. <b><i>Methods</i>:</b> In this study, we characterized the expression profiles of mRNA and nonconding RNAs (ncRNAs) and constructed the regulatory networks based on RNA sequencing in PARPi Olaparib-induced ovarian cancer cells. <b><i>Results</i>:</b> We found that the functions of the differentially expressed genes were enriched in \"PI3K/AKT signaling pathway,\" \"MAPK signaling pathway\" and \"metabolic process\". The functions of DELs (cis) were enriched in \"Human papillomavirus infection\"\"tight junction\" \"MAPK signaling pathway\". As the central regulator of ceRNAs, the differentially expressed miRNAs were enriched in \"Human papillomavirus infection\" \"MAPK signaling pathway\" \"Ras signaling pathway\". According to the degree of interaction, we identified 3 lncRNAs, 2 circRNAs, 7 miRNAs, and 12 mRNA as the key regulatory ceRNA axis, in which miR-320b was the important mediator. <b><i>Conclusion</i>:</b> Here, we revealed the key regulatory lncRNA (circRNA)-miRNA-mRNA axis and their involved pathways in the PARPi resistant ovarian cancer cells. These findings provide new insights into exploring the ceRNA regulatory networks and developing new targets for PARPi resistance.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/20/a8/CG-23-262.PMC9875538.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10704604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of New Genetic Markers and Methods for Sex Determination of Mouse and Human Cells using Polymerase Chain Reactions and Crude DNA Samples.","authors":"Keyin Zhang,&nbsp;Jianglin Yang,&nbsp;Zhenwei Qin,&nbsp;Tianzu Lu,&nbsp;Didong Lou,&nbsp;Qianchuan Ran,&nbsp;Hai Huang,&nbsp;Shuqiang Cheng,&nbsp;Lucas Zellmer,&nbsp;Hong Ma,&nbsp;Dezhong J Liao","doi":"10.2174/1389202923666220610121344","DOIUrl":"https://doi.org/10.2174/1389202923666220610121344","url":null,"abstract":"<p><p><b><i>Background</i>:</b> The currently available methods for sexing human or mouse cells have weaknesses. Therefore, it is necessary to establish new methods. <b><i>Methods</i>:</b> We used bioinformatics approach to identify genes that have alleles on both the X and Y chromosomes of mouse and human genomes and have a region showing a significant difference between the X and Y alleles. We then used polymerase chain reactions (PCR) followed by visualization of the PCR amplicons in agarose gels to establish these genomic regions as genetic sex markers. <b><i>Results</i>:</b> Our bioinformatics analyses identified eight mouse sex markers and 56 human sex markers that are new, <i>i.e</i>. are previously unreported. Six of the eight mouse markers and 14 of the 56 human markers were verified using PCR and ensuing visualization of the PCR amplicons in agarose gels. Most of the tested and untested sex markers possess significant differences in the molecular weight between the X- and Y-derived PCR amplicons and are thus much better than most, if not all, previously-reported genetic sex markers. We also established several simple and essentially cost-free methods for extraction of crude genomic DNA from cultured cells, blood samples, and tissues that could be used as template for PCR amplification. <b><i>Conclusion</i>:</b> We have established new sex genetic markers and methods for extracting genomic DNA and for sexing human and mouse cells. Our work may also lend some methodological strategies to the identification of new genetic sex markers for other organismal species.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/91/04/CG-23-275.PMC9875541.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10704598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic and Bioinformatic Resources for Perennial Fruit Species.","authors":"Jérôme Grimplet","doi":"10.2174/1389202923666220428102632","DOIUrl":"10.2174/1389202923666220428102632","url":null,"abstract":"<p><p>In the post-genomic era, data management and development of bioinformatic tools are critical for the adequate exploitation of genomics data. In this review, we address the actual situation for the subset of crops represented by the perennial fruit species. The agronomical singularity of these species compared to plant and crop model species provides significant challenges on the implementation of good practices generally not addressed in other species. Studies are usually performed over several years in non-controlled environments, usage of rootstock is common, and breeders heavily rely on vegetative propagation. A reference genome is now available for all the major species as well as many members of the economically important genera for breeding purposes. Development of pangenome for these species is beginning to gain momentum which will require a substantial effort in term of bioinformatic tool development. The available tools for genome annotation and functional analysis will also be presented.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":1.8,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5b/a8/CG-23-217.PMC9875543.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10697512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential Role of Plastome Copy Number as a Quality Biomarker for Plant Products using Real-time Quantitative Polymerase Chain Reaction.","authors":"Amita Pandey,&nbsp;Shifa Chaudhary,&nbsp;Binu Bhat","doi":"10.2174/1389202923666220513111643","DOIUrl":"https://doi.org/10.2174/1389202923666220513111643","url":null,"abstract":"<p><p><b><i>Background</i>:</b> Plastids are plant-specific semi-autonomous self-replicating organelles, containing circular DNA molecules called plastomes. Plastids perform crucial functions, including photosynthesis, stress perception and response, synthesis of metabolites, and storage. The plastome and plastid numbers have been shown to be modulated by developmental stage and environmental stimuli and have been used as a biomarker (identification of plant species) and biosensor (an indicator of abiotic and biotic stresses). However, the determination of plastome sequence and plastid number is a laborious process requiring sophisticated equipment. <b><i>Methods</i>:</b> This study proposes using plastome copy number (PCN), which can be determined rapidly by real-time quantitative polymerase chain reaction (RT-qPCR) as a plant product quality biomarker. This study shows that the PCN log₁₀ and range PCN log₁₀ values calculated from RT-qPCR data, which was obtained for two years from leaves and lint samples of cotton and seed samples of cotton, rice, soybean, maize, and sesame can be used for assessing the quality of the samples. <b><i>Results</i>:</b> Observation of lower range PCN log₁₀ values for CS (0.31) and CR (0.58) indicated that the PCN showed little variance from the mean PCN log₁₀ values for CS (3.81) and CR (3.85), suggesting that these samples might have encountered ambient environmental conditions during growth and/ or post-harvest storage and processing. This conclusion was further supported by observation of higher range PCN log₁₀ values for RS (3.09) <i>versus</i> RP (0.05), where rice seeds in the RP group had protective hull covering compared to broken hull-less seeds in the RS group. To further support that PCN is affected by external factors, rice seeds treated with high temperatures and pathogens exhibited lower PCN values when compared to untreated seeds. Furthermore, the range PCN log₁₀ values were found to be high for cotton leaf (CL) and lint (Clt) sample groups, 4.11 and 3.63, respectively, where leaf and lint samples were of different sizes, indicating that leaf samples might be of different developmental stage and lint samples might have been processed differently, supporting that the PCN is affected by both internal and external factors, respectively. Moreover, PCN log₁₀ values were found to be plant specific, with oil containing seeds such as SeS (6.49) and MS (5.05) exhibiting high PCN log₁₀ values compared to non-oil seeds such as SS (1.96). <b><i>Conclusion</i>:</b> In conclusion, it was observed that PCN log₁₀ values calculated from RT-qPCR assays were specific to plant species and the range of PCN log₁₀ values can be directly correlated to the internal and external factors and, therefore might be used as a potential biomarker for assessing the quality of plant products.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/39/51/CG-23-289.PMC9875542.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10697510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long Non-coding RNAs: Pivotal Epigenetic Regulators in Diabetic Retinopathy.","authors":"Zhaoxia Song,&nbsp;Chang He,&nbsp;Jianping Wen,&nbsp;Jianli Yang,&nbsp;Peng Chen","doi":"10.2174/1389202923666220531105035","DOIUrl":"https://doi.org/10.2174/1389202923666220531105035","url":null,"abstract":"<p><p>Diabetic retinopathy (DR) is a severe complication of diabetes; however, its mechanism is not fully understood. Evidence has recently revealed that long non-coding RNAs (lncRNAs) are abnormally expressed in DR, and lncRNAs may function as pivotal regulators. LncRNAs are able to modulate gene expression at the epigenetic level by acting as scaffolds of histone modification complexes and sponges of binding with microRNAs (miRNAs). LncRNAs are believed to be important epigenetic regulators, which may become beneficial in the diagnosis and therapy of DR. However, the mechanisms of lncRNAs in DR are still unclear. In this review, we summarize the possible functions and mechanisms of lncRNAs in epigenetic regulation to target genes in the progression of DR.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c3/31/CG-23-246.PMC9875540.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10697511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancement in Deep Learning Methods for Diagnosis and Prognosis of Cervical Cancer.","authors":"Akshat Gupta,&nbsp;Alisha Parveen,&nbsp;Abhishek Kumar,&nbsp;Pankaj Yadav","doi":"10.2174/1389202923666220511155939","DOIUrl":"https://doi.org/10.2174/1389202923666220511155939","url":null,"abstract":"<p><p>Cervical cancer is the leading cause of death in women, mainly in developing countries, including India. Recent advancements in technologies could allow for more rapid, cost-effective, and sensitive screening and treatment measures for cervical cancer. To this end, deep learning-based methods have received importance for classifying cervical cancer patients into different risk groups. Furthermore, deep learning models are now available to study the progression and treatment of cancerous cervical conditions. Undoubtedly, deep learning methods can enhance our knowledge toward a better understanding of cervical cancer progression. However, it is essential to thoroughly validate the deep learning-based models before they can be implicated in everyday clinical practice. This work reviews recent development in deep learning approaches employed in cervical cancer diagnosis and prognosis. Further, we provide an overview of recent methods and databases leveraging these new approaches for cervical cancer risk prediction and patient outcomes. Finally, we conclude the state-of-the-art approaches for future research opportunities in this domain.</p>","PeriodicalId":10803,"journal":{"name":"Current Genomics","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2022-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f8/f3/CG-23-234.PMC9875539.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10704600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}