Acta Physiologica最新文献

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Deuterated water (2H2O) can be used to quantify hemoglobin synthesis and red blood cell lifespan in humans 氘化水(2H2O)可用于定量人体血红蛋白合成和红细胞寿命。
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-12-20 DOI: 10.1111/apha.14259
Hilkka Kontro, Chris McGlory, Martin J. MacInnis
{"title":"Deuterated water (2H2O) can be used to quantify hemoglobin synthesis and red blood cell lifespan in humans","authors":"Hilkka Kontro, Chris McGlory, Martin J. MacInnis","doi":"10.1111/apha.14259","DOIUrl":"10.1111/apha.14259","url":null,"abstract":"","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 2","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Of manuscripts and memories: Passing the pen to Tobias 手稿和记忆:把笔交给托拜厄斯。
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-12-19 DOI: 10.1111/apha.14255
Pontus B. Persson
{"title":"Of manuscripts and memories: Passing the pen to Tobias","authors":"Pontus B. Persson","doi":"10.1111/apha.14255","DOIUrl":"10.1111/apha.14255","url":null,"abstract":"<p>Imagine awarding authors one of the highest honors in scientific publishing, only to receive an insult in response. Picture writing to inform them they've won the US$100000 <i>Acta Physiologica</i> Award, and instead of gratitude, getting a fiery phone call. Initially, the author thought it was a prank. But once the truth sunk in, it was amusing to witness the quick change in tone—scrambling to recover and secure the award.</p><p>Reflecting on 12 years as Editor-in-Chief of <i>Acta Physiologica</i>, the predominant feeling is one of the deep gratitude and pride. The long journey transformed the journal, elevating its readership, recognition, and scientific impact to new heights. This success is due to the unwavering dedication of our authors, the expertise of our reviewers, the editorial team's rigor, and the enthusiasm of our readers. Together, we've shaped <i>Acta Physiologica</i> into an internationally respected journal that pushes the field of physiology ever forward.</p><p>Today, the editorial team and I are thrilled to welcome Professor Tobias Wang as the new Editor-in-Chief. A distinguished physiologist from Aarhus University, Tobias brings a remarkable depth of experience and an impressive record of achievements. His pioneering work in respiratory and comparative physiology, along with his insights into thermoregulation, make the foremost voice in the field, as seen in his acclaimed articles and television appearances. Having collaborated with Tobias on our editorial team, it's clear he has the vision and drive to lead <i>Acta Physiologica</i> to even greater success. He is the force behind our journal's latest developments.</p><p>We are all in great debt to our previous expert editors Joakim Ek, Lena Eliasson, Karl-Heinz Herzig, Tadashi Isa, Sari Lauri, Bridgit Lumb, Mikko Nikinmaa, Mia Phillipson, and Ursula Seidler. Naturally, the true Chief Editor of this term, Carola Neubert is unforgotten. Inside the eye of the tornado, she makes order out of chaos and will stay onboard with Tobias.</p><p>Excuse me Peter (Peter Bie) for being such a pain in the neck at times. You are the one I thank most for providing me with the honor of becoming <i>Acta Physiologica</i>'s Chief Editor and you are the one that bailed me out when my visions got out of hand. Besides that, you were the first expert editor for kidney physiology during my term and a great friend.</p><p>Together, these extraordinary scientists have shaped <i>Acta Physiologica</i> to reflect the very best of physiology across its diverse fields. Alongside our broader community of readers and contributors, they have transformed the journal over the past decade.</p><p>As the official journal of the Scandinavian Physiological Society (SPS), <i>Acta Physiologica</i> fulfills a unique mission. The SPS, a charitable nonprofit, reinvests journal profits back into the physiological community—funding travel grants, symposia, awards, and more. Being part of a journal that prioritizes the growth","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 1","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.14255","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142851617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Targeting histone deacetylase 6 (HDAC6) in Duchenne muscular dystrophy: New insights into therapeutic potential 靶向组蛋白去乙酰化酶6 (HDAC6)治疗杜氏肌营养不良:治疗潜力的新见解
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-12-16 DOI: 10.1111/apha.14256
Alexis Osseni, Laurent Schaeffer
{"title":"Targeting histone deacetylase 6 (HDAC6) in Duchenne muscular dystrophy: New insights into therapeutic potential","authors":"Alexis Osseni, Laurent Schaeffer","doi":"10.1111/apha.14256","DOIUrl":"10.1111/apha.14256","url":null,"abstract":"<p>Rodney and colleagues provide compelling evidence for the therapeutic potential of selective histone deacetylase 6 (HDAC6) inhibition in <i>mdx</i> mice, a widely used model of Duchenne muscular dystrophy (DMD).<span><sup>1</sup></span> Their study reveals that HDAC6 inhibition promotes enhanced autophagy through increased tubulin acetylation, offering new hope for treatment strategies targeting this critical enzyme. This research sheds light on the potential of HDAC6 inhibitors to address some of the key pathological features of DMD.</p><p>Duchenne muscular dystrophy is a severe, progressive neuromuscular disorder caused by mutations in the dystrophin gene on the X chromosome.<span><sup>2</sup></span> Affecting approximately 1 in 3500 male births, DMD leads to the absence of dystrophin, a structural protein that connects muscle fibers to the extracellular matrix. Without dystrophin, muscle cells are vulnerable to damage and progressive degeneration. DMD typically presents in early childhood, with delayed motor milestones, muscle weakness, and difficulty standing. As the disease progresses, children develop a characteristic waddling gait, difficulty climbing stairs, and progressive muscle loss, ultimately leading to wheelchair dependence by age 12. Complications such as skeletal deformities, breathing difficulties, and cardiomyopathy arise, and most patients do not survive beyond their 30s due to respiratory and cardiac failure.</p><p>Despite two decades of research, no cure for DMD exists, and current treatments remain limited to glucocorticoid therapy. Although innovative genetic approaches, such as exon skipping, gene editing with CRISPR/Cas9, and viral vector-mediated dystrophin delivery, show promise, challenges like inconsistent efficacy, off-target effects, and incomplete dystrophin restoration in muscle tissues—especially in the heart—have slowed progress. As a result, a more comprehensive treatment strategy, combining genetic and pharmacological approaches, is likely necessary to address the multifaceted nature of DMD.</p><p>Over the past 20 years, HDAC inhibitors have shown promise in pre-clinical DMD models. Givinostat, a pan-HDAC inhibitor, was recently FDA-approved for its ability to slow disease progression in ambulatory boys with DMD.<span><sup>3</sup></span> However, pan-HDAC inhibitors can have undesirable side effects, including genotoxicity and impaired DNA repair. To mitigate these risks, more selective HDAC inhibitors have been developed, with HDAC6 emerging as a particularly attractive target. HDAC6-specific inhibitors have been shown to have several advantages over pan-HDAC inhibitors, including a lack of severe side effects. For instance, <i>HDAC6 knockout</i> mice do not exhibit significant pathological features, suggesting that selective inhibition of HDAC6 may be safe and beneficial.<span><sup>4</sup></span></p><p>In animal models, HDAC6 inhibition has demonstrated therapeutic effects in a range of disorders, includi","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 1","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.14256","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142826726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A potential link between enteric glia and the pathophysiology of diet-induced obesity and related metabolic diseases 肠胶质细胞与饮食引起的肥胖和相关代谢疾病的病理生理之间的潜在联系。
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-12-06 DOI: 10.1111/apha.14258
Onesmo B. Balemba, Brian D. Gulbransen
{"title":"A potential link between enteric glia and the pathophysiology of diet-induced obesity and related metabolic diseases","authors":"Onesmo B. Balemba, Brian D. Gulbransen","doi":"10.1111/apha.14258","DOIUrl":"10.1111/apha.14258","url":null,"abstract":"<p>Enteric glia are a large population of peripheral neuroglia that accompany neurons in the enteric nervous system. These cells have diverse functions and engage in bidirectional communication with various cell types, including enteric neurons, immune cells, and possibly the gut microbiota.<span><sup>1, 2</sup></span> Enteric glia play important roles in maintaining gastrointestinal (GI) homeostasis, and it is thought that alterations in their functions could be pivotal in the development of GI disorders. For instance, gains or losses in glial functions contribute to abnormal gut barrier function, inflammation, immune activation, and motor control. Understanding mechanisms by which enteric glia serve as “guardians” of the mucosal barrier has been an area of considerable interest; however, their involvement in mucosal barrier dysfunction is still debated.<span><sup>3</sup></span></p><p>Inflammation caused by altered diet–gut microbiome–host interactions is considered an important driver of increased epithelial permeability in the development of obesity; yet the underlying mechanisms remain poorly understood.<span><sup>4, 5</sup></span> A recent study by D'Antongiovanni et al.<span><sup>6</sup></span> in Acta Physiologica Volume 240 addressed this issue by exploring potential contributions of enteric glia in gut barrier dysfunction driven by ingesting a Western (high-fat) diet. This study specifically focused on potential roles of inflammasome activation in glia as a potential contributor to diet-induced inflammation. The investigators approached this question using wild-type C57BL/6J and NLRP3-KO<sup>−/−</sup> mice fed a 60-kcal high-fat diet (HFD) or standard diet for 8 weeks and studied mucosal integrity by histology, immunolabeling, and western blot. Potential reactive gliosis processes and inflammasome activation were assessed by immunolabeling for glial fibrillary acidic protein (GFAP) and co-labeling for inflammasome components.</p><p>The data show that mice consuming a HFD for 8 weeks increased body weight, altered colon mucus composition by decreasing acidic mucins, disrupted epithelial barrier integrity, increased GFAP-positive glial cells (gliosis), and triggered NLRP3 inflammasome activation. Surprisingly, HFD-NLRP3<sup>−/−</sup> mice failed to gain weight on the HFD and did not exhibit signs of enteric gliosis or altered mucus composition and epithelial barrier integrity. Based on these results, the authors suggested that inflammasome activation is involved in causing obesity, impairing the mucosal barrier, and activating gliosis. To test this concept more directly, the investigators turned to in vitro coculture experiments with a rat-transformed cell line used to model enteric glia (CRL-2690) and a rat intestinal epithelial cell (IEC) line. Challenging cultures with a combination of lipopolysaccharide (LPS) and palmitate was then used to broadly test whether dietary saturated fatty acids and endotoxins disrupt the epithelial barrier ","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 1","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.14258","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142783358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Did you know: Kangaroos are resistant to ventricular arrhythmia 你知道吗?袋鼠对室性心律失常有抵抗力。
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-12-05 DOI: 10.1111/apha.14257
Kirstine Calloe, Stefan M Sattler, Julie Norup Hertel, Carsten Grøndahl, Stamatios Alan Tahas, Morten B. Thomsen
{"title":"Did you know: Kangaroos are resistant to ventricular arrhythmia","authors":"Kirstine Calloe, Stefan M Sattler, Julie Norup Hertel, Carsten Grøndahl, Stamatios Alan Tahas, Morten B. Thomsen","doi":"10.1111/apha.14257","DOIUrl":"10.1111/apha.14257","url":null,"abstract":"","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 1","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142779025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chloride fluxes and GABA release sustain inhibition in the CNS: The role for Bestrophin 1 anion channels 氯离子通量和 GABA 释放维持中枢神经系统的抑制作用:Bestrophin 1 阴离子通道的作用。
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-11-26 DOI: 10.1111/apha.14254
Alexei Verkhratsky, Verena Untiet, Vladimir V. Matchkov
{"title":"Chloride fluxes and GABA release sustain inhibition in the CNS: The role for Bestrophin 1 anion channels","authors":"Alexei Verkhratsky, Verena Untiet, Vladimir V. Matchkov","doi":"10.1111/apha.14254","DOIUrl":"10.1111/apha.14254","url":null,"abstract":"<p>In the current issue of <i>Acta Physiologica</i>, Di Papma et al.<span><sup>1</sup></span> revealed a widespread brain expression of Ca<sup>2+</sup>-dependent anion (chloride) channel Bestrophin 1 (Best1) in both neurones and neuroglia. Chloride ions (Cl<sup>−</sup>) are indispensable for ionotropic inhibition of neurons in the central nervous system (CNS). This inhibition is mainly mediated by GABA<sub>A</sub> and glycine pentameric receptors, the ligand-gated anion channels. Thus, controlling Cl<sup>−</sup> homeostasis is paramount for balancing inhibition and excitation in the nervous circuits, which is critical for CNS function. An aberrant inhibition in the nervous circuits leads to many neurological and neuropsychiatric diseases, including epilepsy and mood disorders.<span><sup>2, 3</sup></span></p><p>Homeostasis of Cl<sup>−</sup> in the CNS is functionally segregated between neurones and astrocytes. In the mature brain, neurones keep cytoplasmic Cl<sup>−</sup> concentration ([Cl<sup>−</sup>]<sub><i>i</i></sub>) low at around ~5–10 mM, while astrocytes maintain high [Cl<sup>−</sup>]<sub><i>i</i></sub> in the range of 30–60 mM.<span><sup>4</sup></span> This disparity defines the functional outcome of the opening of anion channels: in neurones an opening of anion channels mediates Cl<sup>−</sup> influx (which results in hyperpolarization which inhibits neuronal activity), whereas in astrocytes these channels mediate depolarising Cl<sup>−</sup> efflux. Such an opposite arrangement of the [Cl<sup>−</sup>]<sub><i>i</i></sub> homeostasis is critical for maintaining synaptic and extrasynaptic neuronal inhibition. That is, Cl<sup>−</sup> influx into neurones may deplete Cl<sup>−</sup> from the extracellular space but Cl<sup>-</sup> is replenished by a continuous supply of Cl<sup>−</sup> ions from astrocytes.<span><sup>5</sup></span> This coordinated Cl<sup>−</sup> movement between cells and extracellular space is greatly facilitated by a close synaptic association of neuronal and astrocytic compartments, which form a multipartite synapse and a synaptic cradle.<span><sup>6</sup></span> At the inhibitory synapses, the postsynaptic neuronal specialization, as well as astrocytic perisynaptic leaflets, possess GABA<sub>A</sub> receptors.<span><sup>5</sup></span> Hence, presynaptic GABA release opens anion channels in both neuronal and astrocytic membranes. Considering that extracellular Cl<sup>−</sup> concentration can be less than the presumed 120 mM,<span><sup>7</sup></span> astrocytic Cl<sup>−</sup> supply is critical for sustaining inhibitory synaptic transmission. Indeed, optogenetic manipulations with astrocytic [Cl<sup>−</sup>]<sub><i>i</i></sub> substantially affect neuronal inhibition.<span><sup>4</sup></span></p><p>Another key player in Cl<sup>−</sup> homeostasis in the brain tissue is represented by Ca<sup>2+</sup>-activated Cl<sup>−</sup> channels that link together cells excitation, expressed as an intracellular Ca<sup>2+</sup> raise, an","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 1","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.14254","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correction to “Beneficial effects of MGL-3196 and BAM15 combination in a mouse model of fatty liver disease” 更正 "MGL-3196 和 BAM15 联合疗法在脂肪肝小鼠模型中的益处"。
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-11-12 DOI: 10.1111/apha.14250
{"title":"Correction to “Beneficial effects of MGL-3196 and BAM15 combination in a mouse model of fatty liver disease”","authors":"","doi":"10.1111/apha.14250","DOIUrl":"10.1111/apha.14250","url":null,"abstract":"<p>Zhou, M., Li, C., Byrne, F. L., Vancuylenburg, C. S., Olzomer, E. M., Hargreaves, A., Wu, L. E., Shackel, N. A., Santos, W. L., &amp; Hoehn, K. L. Beneficial effects of MGL-3196 and BAM15 combination in a mouse model of fatty liver disease. <i>Acta Physiologica</i>. 2014; 240(10): e14217. https://doi.org/10.1111/apha.14217</p><p>In Figure 1A, the compound structure of MGL-3196 is incorrect due to an extra bond between the Cl and N. The corrected structure (below) has the bond between the Cl and N removed.</p>","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 1","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.14250","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142612905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impaired suppression of fatty acid release by insulin is a strong predictor of reduced whole-body insulin-mediated glucose uptake and skeletal muscle insulin receptor activation 胰岛素对脂肪酸释放的抑制作用受损是全身胰岛素介导的葡萄糖摄取和骨骼肌胰岛素受体活化减少的一个有力预测因素。
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-11-01 DOI: 10.1111/apha.14249
Michael W. Schleh, Benjamin J. Ryan, Cheehoon Ahn, Alison C. Ludzki, Douglas W. Van Pelt, Lisa M. Pitchford, Olivia K. Chugh, Austin T. Luker, Kathryn E. Luker, Dmitri Samovski, Nada A. Abumrad, Charles F. Burant, Jeffrey F. Horowitz
{"title":"Impaired suppression of fatty acid release by insulin is a strong predictor of reduced whole-body insulin-mediated glucose uptake and skeletal muscle insulin receptor activation","authors":"Michael W. Schleh,&nbsp;Benjamin J. Ryan,&nbsp;Cheehoon Ahn,&nbsp;Alison C. Ludzki,&nbsp;Douglas W. Van Pelt,&nbsp;Lisa M. Pitchford,&nbsp;Olivia K. Chugh,&nbsp;Austin T. Luker,&nbsp;Kathryn E. Luker,&nbsp;Dmitri Samovski,&nbsp;Nada A. Abumrad,&nbsp;Charles F. Burant,&nbsp;Jeffrey F. Horowitz","doi":"10.1111/apha.14249","DOIUrl":"10.1111/apha.14249","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To examine factors underlying why most, but not all, adults with obesity exhibit impaired insulin-mediated glucose uptake, we compared: (1) adipose tissue fatty acid (FA) release, (2) skeletal muscle lipid droplet (LD) characteristics, and (3) insulin signalling events, in skeletal muscle of adults with obesity with relatively high versus low insulin-mediated glucose uptake.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Seventeen adults with obesity (BMI: 36 ± 3 kg/m<sup>2</sup>) completed a 2 h hyperinsulinemic–euglycemic clamp with stable isotope tracer infusions to measure glucose rate of disappearance (glucose Rd) and FA rate of appearance (FA Ra). Skeletal muscle biopsies were collected at baseline and 30 min into the insulin infusion. Participants were stratified into HIGH (<i>n</i> = 7) and LOW (<i>n</i> = 10) insulin sensitivity cohorts by their glucose Rd during the hyperinsulinemic clamp (LOW&lt; 400; HIGH &gt;550 nmol/kgFFM/min/[μU/mL]).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Insulin-mediated suppression of FA Ra was lower in LOW compared with HIGH (<i>p</i> &lt; 0.01). In skeletal muscle, total intramyocellular lipid content did not differ between cohorts. However, the size of LDs in the subsarcolemmal region (SS) of type II muscle fibres was larger in LOW compared with HIGH (<i>p</i> = 0.01). Additionally, insulin receptor-β (IRβ) interactions with regulatory proteins CD36 and Fyn were lower in LOW versus HIGH (<i>p</i> &lt; 0.01), which aligned with attenuated insulin-mediated Tyr phosphorylation of IRβ and downstream insulin-signalling proteins in LOW.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Collectively, reduced ability for insulin to suppress FA mobilization, with accompanying modifications in intramyocellular LD size and distribution, and diminished IRβ interaction with key regulatory proteins may be key contributors to impaired insulin-mediated glucose uptake commonly found in adults with obesity.</p>\u0000 </section>\u0000 </div>","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"241 1","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Differential production of mitochondrial reactive oxygen species between mouse (Mus musculus) and crucian carp (Carassius carassius) 小鼠(Mus musculus)和鲫鱼(Carassius carassius)线粒体活性氧产生的差异。
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-10-28 DOI: 10.1111/apha.14244
Lucie Gerber, May-Kristin Torp, Göran E. Nilsson, Sjannie Lefevre, Kåre-Olav Stensløkken
{"title":"Differential production of mitochondrial reactive oxygen species between mouse (Mus musculus) and crucian carp (Carassius carassius)","authors":"Lucie Gerber,&nbsp;May-Kristin Torp,&nbsp;Göran E. Nilsson,&nbsp;Sjannie Lefevre,&nbsp;Kåre-Olav Stensløkken","doi":"10.1111/apha.14244","DOIUrl":"10.1111/apha.14244","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>In most vertebrates, oxygen deprivation and subsequent re-oxygenation are associated with mitochondrial impairment and excess production of reactive oxygen species (ROS) like hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>). This in turn triggers a cascade of cell-damaging events in a temperature-dependent manner. The crucian carp (<i>Carassius carassius</i>) is one of few vertebrates that survives months without oxygen at cold temperatures and overcomes oxidative damage during re-oxygenation periods. Mitochondria of this anoxia-tolerant species therefore serve as an excellent model in translational research to study adaptation and resilience to low oxygen conditions and thermal variability.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Here, we used high-resolution respirometry on isolated mitochondria from hearts of crucian carp and the anoxia-intolerant mouse (<i>Mus musculus</i>), at 37 and 8°C; two temperatures relevant for transplantation medicine (i.e., graft preservation and subsequent rewarming).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>We find: (1) a striking difference in H<sub>2</sub>O<sub>2</sub> release between the two species at 37°C despite comparable mitochondrial efficiency and capacity, (2) a massive H<sub>2</sub>O<sub>2</sub> release after inhibition of complex V in mouse at 37°C that is absent in crucian carp, and prevented in mouse by incubation at 8°C or uncoupling with a protonophore at 37°C, and (3) indications that differences in mitochondrial complex I and II capacity and thermal sensitivity influence the release of mitochondrial H<sub>2</sub>O<sub>2</sub> relative to respiration.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Our findings provide comparative insights into a spectrum of mitochondrial adaptations in vertebrates and the importance of thermal variability. Furthermore, the species- and temperature-related changes associated with mitochondria highlighted in this study may help identify mitochondria-based targets for translational medicine.</p>\u0000 </section>\u0000 </div>","PeriodicalId":107,"journal":{"name":"Acta Physiologica","volume":"240 12","pages":""},"PeriodicalIF":5.6,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/apha.14244","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A quantitative analysis of bestrophin 1 cellular localization in mouse cerebral cortex 小鼠大脑皮层中 bestrophin 1 细胞定位的定量分析
IF 5.6 2区 医学
Acta Physiologica Pub Date : 2024-10-28 DOI: 10.1111/apha.14245
Michael Di Palma, Wuhyun Koh, C. Justin Lee, Fiorenzo Conti
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