Crop Journal最新文献

{"title":"Development of plant cytosine base editors with the Cas12a system","authors":"Huanhuan Wang ,&nbsp;Jing Liang ,&nbsp;Like Chen ,&nbsp;Bufang Deng ,&nbsp;Dongfang Gu ,&nbsp;Xiaoshuang Liu ,&nbsp;Shan Jin ,&nbsp;Rongfang Xu ,&nbsp;Ruiying Qin ,&nbsp;Yitong Zhu ,&nbsp;Liangxia Zhao ,&nbsp;Dourong Kou ,&nbsp;Yanjun Chen ,&nbsp;Yingli Jiang ,&nbsp;Juan Li ,&nbsp;Pengcheng Wei","doi":"10.1016/j.cj.2023.03.002","DOIUrl":"https://doi.org/10.1016/j.cj.2023.03.002","url":null,"abstract":"<div><p>Base editors of the Cas9 system have been widely used for precise nucleotide substitution in crops. In this study, Cas12a was applied to construct plant cytosine base editors (CBEs). The main elements of Cas12a-CBEs were engineered and their efficiency was evaluated in stably transformed rice cells. An optimized ttCas12a-hyA3Bctd editor, consisting of a LbCas12a variant carrying catalytic inactive D832A and temperature-tolerance D156R double mutations, a truncated human APOBEC3B deaminase, a human RAD51 single-stranded DNA-binding domain, and double copies of UGI, outperformed other Cas12a-CBEs in base editing efficiency. In T₀ transgenic rice plants, ttCas12a-hyA3Bctd edited an average of 42.01% and a maximum of 68.75% of lines at six genomic targets. A-to-G conversions were generated in rice by an adenine base editor with a similar architecture to the optimized CBE. Our results provide preliminary evidence for the feasibility of robust and efficient plant Cas12a base editing systems, which could be useful for precise crop breeding.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1451-1457"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49845241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple omics datasets reveal significant physical and physiological dormancy in alfalfa hard seeds identified by multispectral imaging analysis","authors":"Xuemeng Wang ,&nbsp;Han Zhang ,&nbsp;Rui Song,&nbsp;Ming Sun,&nbsp;Ping Liu,&nbsp;Peixin Tian,&nbsp;Peisheng Mao,&nbsp;Shangang Jia","doi":"10.1016/j.cj.2023.03.003","DOIUrl":"https://doi.org/10.1016/j.cj.2023.03.003","url":null,"abstract":"<div><p>Physical dormancy (PY) commonly present in the seeds of higher plants is believed to be responsible for the germination failure by impermeable seed coat in hard seeds of legume species, instead of physiological dormancy (PD). In this study, a non-destructive approach involving multispectral imaging was used to successfully identify hard seeds from non-hard seeds in <em>Medicago sativa</em>, with accuracy as high as 96.8%–99.0%. We further adopted multiple-omics strategies to investigate the differences of physiology, metabolomics, methylomics, and transcriptomics in alfalfa hard seeds, with non-hard seeds as control. The hard seeds showed dramatically increased antioxidants and 125 metabolites of significant differences in non-targeted metabolomics analysis, which are enriched in the biosynthesis pathways of flavonoids, lipids and hormones, especially with significantly higher ABA, a hormone known to induce dormancy. In our transcriptomics results, the enrichment pathway of “response to abscisic acid” of differential expressed genes (DEG) supported the key role of ABA in metabolomics results. The methylome analysis identified 54,899, 46,216 and 54,452 differential methylation regions for contexts of CpG, CHG and CHH, and 344 DEGs might be regulated by hypermethylation and hypomethylation of promoter and exon regions, including four ABA- and JA-responsive genes. Among 8% hard seeds in seed lots, 24.5% still did not germinate after scarifying seed coat, and were named as non-PY hard seeds. Compared to hard seeds, significantly higher contents of ABA/IAA and ABA/JA were identified in non-PY hard seeds, which indicated the potential presence of PD. In summary, the significantly changed metabolites, gene expressions, and methylations all suggested involvement of ABA responses in hard seeds, and germination failure of alfalfa hard seeds was caused by combinational dormancy (PY + PD), rather than PY alone.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1458-1468"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49845242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reversible protein phosphorylation, a central signaling hub to regulate carbohydrate metabolic networks","authors":"Fengyi Gao ,&nbsp;Liang Zhang ,&nbsp;James R. Lloyd ,&nbsp;Wenbin Zhou ,&nbsp;Yanmei Chen","doi":"10.1016/j.cj.2023.05.014","DOIUrl":"https://doi.org/10.1016/j.cj.2023.05.014","url":null,"abstract":"<div><p>Plants produce a range of carbohydrates to meet their growth and developmental needs. Protein reversible phosphorylation plays key roles in coordinating multiple metabolic pathways and integrating diverse internal and external cues. Understanding such regulatory metabolism will provide novel resources for breeding and crop management by modulating metabolic pathways for control of growth and stress response. In this review, we summarize the complex, multifaceted functions of protein phosphorylation and their connections to plant metabolism. We focus particularly on carbohydrate metabolic pathways that are controlled by key kinases and discuss how they are linked to downstream changes in physiology, important agronomic traits and crop quality.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1279-1286"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49883482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FGW1, a protein containing DUF630 and DUF632 domains, regulates grain size and filling in Oryza sativa L.","authors":"Yangyang Li ,&nbsp;Peilong He ,&nbsp;Xiaowen Wang ,&nbsp;Hongyan Chen ,&nbsp;Jile Ni ,&nbsp;Weijiang Tian ,&nbsp;Xiaobo Zhang ,&nbsp;Zhibo Cui ,&nbsp;Guanghua He ,&nbsp;Xianchun Sang","doi":"10.1016/j.cj.2023.03.015","DOIUrl":"https://doi.org/10.1016/j.cj.2023.03.015","url":null,"abstract":"<div><p>Grain filling influences grain size and quality in cereal crops. The molecular mechanisms that regulate grain endosperm development remain elusive. In this study, we characterized a <em>filling-defective</em> and <em>grain width</em> mutant, <em>fgw1</em>, whose mutation increased rice seed width mainly via cell division and expansion in grains. Sucrose contents were higher but starch contents lower in the <em>fgw1</em> mutant during the grain-filling stage, resulting in inferior endosperm of opaque, white appearance with loosely packed starch granules. Map-based cloning revealed that <em>FGW1</em> encoded a protein containing DUF630/DUF632 domains, localized in the plasma membrane with preferential expression in the panicle. RNA interference in <em>FGW1</em> resulted in increased grain width and weight, whereas overexpression of <em>FGW1</em> led to slightly narrower kernels and better grain filling. In a yeast two-hybrid assay, FGW1 interacted directly with the 14–3–3 protein GF14f, bimolecular fluorescence complementation verified that the site of interaction was the membrane, and the mutated FGW1 protein failed to interact with GF14f. The expression of GF14f was down-regulated in <em>fgw1</em>, and the activities of AGPase, StSase, and SuSase in the endosperm of <em>fgw1</em> increased similarly to those of a reported GF14f-RNAi. Transcriptome analysis indicated that <em>FGW1</em> also regulates cellular processes and carbohydrate metabolism. Thus, FGW1 regulated grain formation via the GF14f pathway.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1390-1400"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49883486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The R2R3-MYB transcription factor GaPC controls petal coloration in cotton","authors":"Caiping Cai ,&nbsp;Fan Zhou ,&nbsp;Weixi Li ,&nbsp;Yujia Yu ,&nbsp;Zhihan Guan ,&nbsp;Baohong Zhang ,&nbsp;Wangzhen Guo","doi":"10.1016/j.cj.2023.03.013","DOIUrl":"https://doi.org/10.1016/j.cj.2023.03.013","url":null,"abstract":"<div><p>Although a few cases of genetic epistasis in plants have been reported, the combined analysis of genetically phenotypic segregation and the related molecular mechanism remains rarely studied. Here, we have identified a gene (named <em>GaPC</em>) controlling petal coloration in <em>Gossypium arboreum</em> and following a heritable recessive epistatic genetic model. Petal coloration is controlled by a single dominant gene, <em>GaPC</em>. A loss-of-function mutation of <em>GaPC</em> leads to a recessive gene <em>Gapc</em> that masks the phenotype of other color genes and shows recessive epistatic interactions. Map-based cloning showed that <em>GaPC</em> encodes an R2R3-MYB transcription factor. A 4814-bp long terminal repeat retrotransposon insertion at the second exon led to <em>GaPC</em> loss of function and disabled petal coloration. <em>GaPC</em> controlled petal coloration by regulating the anthocyanin and flavone biosynthesis pathways. Expression of core genes in the phenylpropanoid and anthocyanin pathways was higher in colored than in white petals. Petal color was conferred by flavonoids and anthocyanins, with red and yellow petals rich in anthocyanin and flavonol glycosides, respectively. This study provides new insight on molecular mechanism of recessive epistasis, also has potential breeding value by engineering <em>GaPC</em> to develop colored petals or fibers for multi-functional utilization of cotton.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1319-1330"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49883483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruption of LEAF LESION MIMIC 4 affects ABA synthesis and ROS accumulation in rice","authors":"Hao Wu ,&nbsp;Gaoxing Dai ,&nbsp;Rao Yuchun ,&nbsp;Kaixiong Wu ,&nbsp;Junge Wang ,&nbsp;Peng Hu ,&nbsp;Yi Wen ,&nbsp;Yueying Wang ,&nbsp;Lixin Zhu ,&nbsp;Bingze Chai ,&nbsp;Jialong Liu ,&nbsp;Guofu Deng ,&nbsp;Qian Qian ,&nbsp;Jiang Hu","doi":"10.1016/j.cj.2023.04.001","DOIUrl":"https://doi.org/10.1016/j.cj.2023.04.001","url":null,"abstract":"<div><p>Lesion mimic mutants (LMMs) are advantageous materials for studying programmed cell death (PCD). Although some rice LMM genes have been cloned, the diversity of functions of these genes indicates that the mechanism of cell death regulation in LMMs needs further study. In this study, we identified a rice light-dependent <em>leaf lesion mimic mutant 4</em> (<em>llm4</em>) that showed abnormal chloroplast structure, photoinhibition, reduced photosynthetic protein levels, massive accumulation of reactive oxygen species (ROS), and PCD. Map-based cloning and complementation testing revealed that <em>LLM4</em> encodes zeaxanthin epoxidase (ZEP), an enzyme involved in the xanthophyll cycle, which functions in plant photoprotection, ROS scavenging, and carotenoid and abscisic acid (ABA) biosynthesis. The ABA content was decreased, and the contents of 24 carotenoids differed between the <em>llm4</em> mutant and the wild type (WT). The <em>llm4</em> mutant showed reduced dormancy and greater sensitive to ABA than the WT. We concluded that the mutation of <em>LLM4</em> resulted in the failure of xanthophyll cycle, in turn causing ROS accumulation. The excessive ROS accumulation damaged chloroplast structure and induced PCD, leading eventually to the formation of lesion mimics.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1341-1352"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49883485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A homeodomain-leucine zipper I transcription factor, MeHDZ14, regulates internode elongation and leaf rolling in cassava (Manihot esculenta Crantz)","authors":"Xiaoling Yu ,&nbsp;Xin Guo ,&nbsp;Pingjuan Zhao ,&nbsp;Shuxia Li ,&nbsp;Liangping Zou ,&nbsp;Wenbin Li ,&nbsp;Ziyin Xu ,&nbsp;Ming Peng ,&nbsp;Mengbin Ruan","doi":"10.1016/j.cj.2023.03.001","DOIUrl":"https://doi.org/10.1016/j.cj.2023.03.001","url":null,"abstract":"<div><p>Drought stress impairs plant growth and other physiological functions. MeHDZ14, a homeodomain-leucine zipper I transcription factor, is strongly induced by drought stress in various cassava cultivars. However, the role of <em>MeHDZ14</em> in cassava growth regulation has remained unclear. Here we report that <em>MeHDZ14</em> affected plant height, such that a dwarf phenotype and altered internode elongation were observed in transgenic cassava lines. <em>MeHDZ14</em> was found to negatively regulate the biosynthesis of lignin. Its overexpression resulted in abaxially rolled leaves. The morphogenesis of leaf epidermal cells was inhibited by overexpression of <em>MeHDZ14</em>, with decreased auxin and gibberellin and increased cytokinin contents. <em>MeHDZ14</em> was found to regulate many drought-responsive genes, including genes involved in cell wall synthesis and expansion. MeHDZ14 bound to the promoter of <em>caffeic acid 3-O-methyltransferase 1</em> (<em>MeCOMT1</em>), acting as a transcriptional repressor of genes involved in cell wall development. <em>MeHDZ14</em> appears to act as a negative regulator of internode elongation and epidermal cell morphogenesis during cassava leaf development.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1419-1430"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49883487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of sorghum mutants with improved in vitro protein digestibility by CRISPR/Cas9 editing of kafirin genes","authors":"Lev A. Elkonin ,&nbsp;Grigoriy A. Gerashchenkov ,&nbsp;Natalie V. Borisenko ,&nbsp;Odyssey A. Kenzhegulov ,&nbsp;Saule Kh. Sarsenova ,&nbsp;Natalya A. Rozhnova ,&nbsp;Valery M. Panin","doi":"10.1016/j.cj.2023.02.005","DOIUrl":"https://doi.org/10.1016/j.cj.2023.02.005","url":null,"abstract":"<div><p>Sorghum (<em>Sorghum bicolor</em> (L.) Moench) is a major world crop that is a reliable source of fodder and food grain in arid regions. However, unlike other cereals, sorghum grain has low nutritional value, owing mainly to the resistance of its storage proteins (kafirins) to protease digestion. Changing the composition of kafirins or their primary structure may address this problem. To induce mutations in kafirin-encoding genes that were expected to disturb their accumulation in endosperm cells, we used a genome-editing approach. By <em>Agrobacterium</em>-mediated genetic transformation of immature embryos of cv. Avans, we obtained 14 transgenic plants with genetic constructs for site-directed mutagenesis of the <em>k1C5</em> and <em>gKAF1</em> genes encoding 22 kDa α- and 28 kDa γ-kafirins, respectively. Sequencing of 5 regenerants obtained by using <em>k1C5</em>-addressing vector revealed two plants with mutations. T₁ progeny of these mutants had higher <em>in vitro</em> digestibility of endosperm proteins (86%–92%), in comparison with the donor Avans (63%–67%). The kernels of these plants had a thick vitreous endosperm. A mutant with increased <em>in vitro</em> protein digestibility and vitreous endosperm, carrying a mutation in the target sequence, was also obtained by use of the <em>gKAF1</em>-addressing vector. Thus, using genome editing technology, we have obtained mutants with improved kafirin digestibility that can be used in sorghum breeding.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1411-1418"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49803787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resequencing-based QTL mapping for yield and resistance traits reveals great potential of Oryza longistaminata in rice breeding","authors":"Weixiong Long ,&nbsp;Nengwu Li ,&nbsp;Jie Jin ,&nbsp;Jie Wang ,&nbsp;Dong Dan ,&nbsp;Fengfeng Fan ,&nbsp;Zhiyong Gao ,&nbsp;Shaoqing Li","doi":"10.1016/j.cj.2023.03.017","DOIUrl":"https://doi.org/10.1016/j.cj.2023.03.017","url":null,"abstract":"<div><p>As a natural genetic reservoir, wild rice contains many favorable alleles and mutations conferring high yield and resistance to biotic and abiotic stresses. However, there are few reports describing favorable genes or QTL from the AA genome wild rice <em>O. longistaminata</em>, which is characterized by tall and robust habit and long tassels and anthers and shows high potential for use in cultivated rice improvement. We constructed a stable BC₂F₂₀ backcross inbred line (BIL) population of 152 lines from the cross of 9311 × <em>O. longistaminat.</em> Some BILs showed large panicles, large seeds, and strong resistance to rice false smut, bacterial leaf blight, rice blast spot, and brown planthopper. Genomic resequencing showed that the 152 BILs covered about 99.6% of the <em>O. longistaminata</em> genome. QTL mapping with 2432 bin markers revealed 13 QTL associated with seven yield traits and eight with resistance to brown planthopper and to four diseases. Of these QTL, 12 for grain yield and 11 for pest and disease resistance are novel in <em>Oryza</em> species. A large-panicle NIL1880 line containing QTL <em>qPB8.1</em> showed a nearly 50% increase in spikelet number and 27.5% in grain yield compared to the recurrent parent 9311. These findings support the potential value of <em>O. longistaminata</em> for cultivated rice improvement.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1541-1549"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49803757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GmNLP7a inhibits soybean nodulation by interacting with GmNIN1a","authors":"Xuesong Wu ,&nbsp;Yuping Xiong ,&nbsp;Jingjing Lu,&nbsp;Mi Yang,&nbsp;Hongtao Ji,&nbsp;Xia Li,&nbsp;Zhijuan Wang","doi":"10.1016/j.cj.2023.03.016","DOIUrl":"https://doi.org/10.1016/j.cj.2023.03.016","url":null,"abstract":"<div><p>Nitrogen (N) is an essential macronutrient for plant growth and productivity. Leguminous plants establish symbiotic relationships with nitrogen-fixing rhizobial bacteria to use atmospheric dinitrogen gas to meet high N demand under low-N conditions. Nodule formation and N fixation are energy-consuming processes and are inhibited by nitrate present in the environment. Previous studies in model leguminous plants characterized NIN-LIKE PROTEIN (NLP) proteins that mediate nitrate control of root nodule symbiosis, but the mechanism by which nitrate regulates soybean root nodules via NLP remains unclear. In the soybean genome we found four homologs of AtNLP7, named GmNLP7a–GmNLP7d. We showed that the expression of <em>GmNLP7s</em> is responsive to nitrate but not to rhizobial infection and localized GmNLP7a to the nucleus. Downregulation of <em>GmNLP7s</em> increased nodule number, and overexpression of <em>GmNLP7a</em> (<em>GmNLP7aOE</em>) reduced nodule number regardless of nitrate availability, suggesting a negative role for GmNLP7s in nodulation. Nitrogenase activity in the <em>GmNLP7aOE</em> line was comparable to that of the wild type, indicating that <em>GmNLP7a</em> does not affect mature nodule activity. Overexpression of <em>GmNLP7a</em> downregulated the expression of <em>GmNIN1a</em> and <em>GmENOD40-1</em>. GmNLP7a interacted with GmNIN1a via the PB1 domain. Our results reveal a new regulator of GmNLP7 in nodulation and a molecular mechanism by which nitrate affects nodule number in soybean.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 5","pages":"Pages 1401-1410"},"PeriodicalIF":6.6,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49845240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}